(Circular) (Six-base) MAPSORT of: pFN21AB8365.seq Check: 2444 from: 1 to: 6121 pFN21AB8365 6121 bp With 182 enzymes: SgfI * February 27, 2009 14:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3931 278 Size: 53 83 186 936 2395 2468 Fragments arranged by size: 2468 2395 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3464 1937 Size: 1527 4594 Acc65I G'GTAC_C Cuts at: 3442 3442 Size: 6121 AclI AA'CG_TT Cuts at: 1563 4248 4621 1563 Size: 2685 373 3063 Fragments arranged by size: 3063 2685 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4184 5568 5836 1632 Size: 2552 1384 268 1917 Fragments arranged by size: 2552 1917 1384 268 AfeI AGC'GCT Cuts at: 2642 2798 2642 Size: 156 5965 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5924 AflIII A'CryG_T Cuts at: 1203 2199 2760 5020 5997 1203 Size: 996 561 2260 977 1327 Fragments arranged by size: 2260 1327 996 977 561 AgeI A'CCGG_T Cuts at: 4952 4952 Size: 6121 AhdI GACnn_n'nnGTC Cuts at: 4850 4850 Size: 6121 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6121 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6121 AlwNI CAG_nnn'CTG Cuts at: 2046 3007 3274 3864 5436 2046 Size: 961 267 590 1572 2731 Fragments arranged by size: 2731 1572 961 590 267 ApaI G_GGCC'C Cuts at: 2390 2988 3096 2390 Size: 598 108 5415 Fragments arranged by size: 5415 598 108 ApaLI G'TGCA_C Cuts at: 2942 4178 5334 2942 Size: 1236 1156 3729 Fragments arranged by size: 3729 1236 1156 ApoI r'AATT_y Cuts at: 1493 3430 3656 3808 5917 1493 Size: 1937 226 152 2109 1697 Fragments arranged by size: 2109 1937 1697 226 152 AscI GG'CGCG_CC Cuts at: 2970 2970 Size: 6121 AseI AT'TA_AT Cuts at: 160 1032 4675 160 Size: 872 3643 1606 Fragments arranged by size: 3643 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6121 AvaI C'yCGr_G Cuts at: 1870 1943 2215 2332 2981 3095 3446 1870 Size: 73 272 117 649 114 351 4545 Fragments arranged by size: 4545 649 351 272 117 114 73 AvrII C'CTAG_G Cuts at: 2221 2221 Size: 6121 BamHI G'GATC_C Cuts at: 3451 3818 3451 Size: 367 5754 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2276 2345 2560 2861 2900 Size: 325 217 627 489 69 215 301 39 Cuts at: 2900 3038 3142 3442 4897 618 Size: 138 104 300 1455 1842 Fragments arranged by size: 1842 1455 627 489 325 301 300 217 215 138 104 69 39 BanII G_rGCy'C Cuts at: 1391 2123 2270 2333 2390 2423 2729 2988 3096 Size: 732 147 63 57 33 306 259 108 Cuts at: 3096 3350 3360 3440 1391 Size: 254 10 80 4072 Fragments arranged by size: 4072 732 306 259 254 147 108 80 63 57 33 10 BbeI G_GCGC'C Cuts at: 2280 2349 2564 2865 2904 3042 2280 Size: 69 215 301 39 138 5359 Fragments arranged by size: 5359 301 215 138 69 39 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2957 3059 928 Size: 971 1058 102 3990 Fragments arranged by size: 3990 1058 971 102 BbvCI CC'TCA_GC Cuts at: 3361 3361 Size: 6121 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2746 2780 4312 4346 1903 Size: 34 809 34 1532 34 3678 Fragments arranged by size: 3678 1532 809 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4013 5223 4013 Size: 1210 4911 BclI T'GATC_A Cuts at: 1548 1548 Size: 6121 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2337 2463 4732 Size: 107 122 71 631 605 665 126 2269 Cuts at: 4732 136 Size: 1525 Fragments arranged by size: 2269 1525 665 631 605 126 122 107 71 BglII A'GATC_T Cuts at: 2589 6116 2589 Size: 3527 2594 BlpI GC'TnA_GC Cuts at: 2327 2582 3538 2327 Size: 255 956 4910 Fragments arranged by size: 4910 956 255 Bme1580I G_kGCm'C Cuts at: 1790 2390 2946 2988 3096 3117 4182 5338 1790 Size: 600 556 42 108 21 1065 1156 2573 Fragments arranged by size: 2573 1156 1065 600 556 108 42 21 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6121 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3852 4810 442 Size: 416 53 83 399 24 2435 958 1753 Fragments arranged by size: 2435 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6121 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2098 2098 Size: 6121 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3042 3389 4781 1239 Size: 1803 347 1392 2579 Fragments arranged by size: 2579 1803 1392 347 Bpu10I CC'TnA_GC Cuts at: 3361 3361 Size: 6121 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4246 5111 5409 4246 Size: 865 298 4958 Fragments arranged by size: 4958 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4784 882 Size: 617 3285 2219 Fragments arranged by size: 3285 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3912 493 Size: 711 2708 2702 Fragments arranged by size: 2708 2702 711 BsaBI GATnn'nnATC Cuts at: 6115 6115 Size: 6121 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2189 2277 2346 2478 Size: 53 83 186 936 656 88 69 132 Cuts at: 2478 2561 2862 2901 3039 3928 4310 4945 275 Size: 83 301 39 138 889 382 635 1451 Fragments arranged by size: 1451 936 889 656 635 382 301 186 138 132 88 83 83 69 53 39 BsaWI w'CCGG_w Cuts at: 4554 4952 5226 5373 5784 4554 Size: 398 274 147 411 4891 Fragments arranged by size: 4891 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2735 2765 3188 3218 3362 Size: 30 165 30 1332 30 423 30 144 Cuts at: 3362 3392 1178 Size: 30 3907 Fragments arranged by size: 3907 1332 423 165 144 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2074 3055 3217 1186 Size: 888 981 162 4090 Fragments arranged by size: 4090 981 888 162 BseYI C'CCAG_C Cuts at: 2564 2912 3156 5324 5820 2564 Size: 348 244 2168 496 2865 Fragments arranged by size: 2865 2168 496 348 244 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2726 3146 1163 Size: 573 990 420 4138 Fragments arranged by size: 4138 990 573 420 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2301 2400 3571 4332 5360 1540 Size: 275 186 300 99 1171 761 1028 2301 Fragments arranged by size: 2301 1171 1028 761 300 275 186 99 BsiHKAI G_wGCw'C Cuts at: 2946 4182 4267 5338 2946 Size: 1236 85 1071 3729 Fragments arranged by size: 3729 1236 1071 85 BsiWI C'GTAC_G Cuts at: 2430 2430 Size: 6121 BsmI GAATG_Cn' Cuts at: 3632 3725 3632 Size: 93 6028 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2123 2270 2333 2390 2423 2729 2946 Size: 399 333 147 63 57 33 306 217 Cuts at: 2946 2988 3096 3117 3350 3360 3440 4182 4267 Size: 42 108 21 233 10 80 742 85 Cuts at: 4267 5338 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 333 306 233 217 147 108 85 80 63 57 42 33 21 10 BspEI T'CCGG_A Cuts at: 5784 5784 Size: 6121 BspHI T'CATG_A Cuts at: 4010 5740 4010 Size: 1730 4391 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3476 844 Size: 2632 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2172 2337 2844 4008 1394 Size: 429 349 165 507 1164 3507 Fragments arranged by size: 3507 1164 507 429 349 165 BsrDI GCAATG_nn' Cuts at: 65 1248 4616 4790 65 Size: 1183 3368 174 1396 Fragments arranged by size: 3368 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2228 2252 2300 2625 2825 2954 2974 Size: 110 418 24 48 325 200 129 20 Cuts at: 2974 3373 4765 4952 1700 Size: 399 1392 187 2869 Fragments arranged by size: 2869 1392 418 399 325 200 187 129 110 48 24 20 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6121 BssHII G'CGCG_C Cuts at: 1925 2970 1925 Size: 1045 5076 BssSI C'ACGA_G Cuts at: 3149 4181 5193 3149 Size: 1032 1012 4077 Fragments arranged by size: 4077 1032 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6121 BstBI TT'CG_AA Cuts at: 4925 4925 Size: 6121 BstEII G'GTnAC_C Cuts at: 1184 3334 1184 Size: 2150 3971 BstYI r'GATC_y Cuts at: 1972 2589 3451 3818 4203 4220 5672 5758 5770 Size: 617 862 367 385 17 1452 86 12 Cuts at: 5770 6116 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 862 617 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2313 2349 2424 2544 513 Size: 552 1248 36 75 120 4090 Fragments arranged by size: 4090 1248 552 120 75 36 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5382 Fragments arranged by size: 5382 683 56 BtsI GCAGTG_nn' Cuts at: 3272 3637 4431 4451 3272 Size: 365 794 20 4942 Fragments arranged by size: 4942 794 365 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6121 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6086 DraI TTT'AAA Cuts at: 3425 3425 Size: 6121 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2188 2687 5128 809 Size: 507 872 499 2441 1802 Fragments arranged by size: 2441 1802 872 507 499 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2298 2310 2397 Size: 54 1425 50 275 18 468 12 87 Cuts at: 2397 2547 2956 3568 4457 6086 8 Size: 150 409 612 889 1629 43 Fragments arranged by size: 1629 1425 889 612 468 409 275 150 87 54 50 43 18 12 EagI C'GGCC_G Cuts at: 1537 1812 2298 2397 3568 1537 Size: 275 486 99 1171 4090 Fragments arranged by size: 4090 1171 486 275 99 EarI CTCTTCn'nnn_ Cuts at: 1353 2618 4051 1353 Size: 1265 1433 3423 Fragments arranged by size: 3423 1433 1265 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2648 4708 5082 5228 387 Size: 1064 138 1059 2060 374 146 1280 Fragments arranged by size: 2060 1280 1064 1059 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3042 3389 4184 4781 5568 5836 1239 Size: 393 1410 347 795 597 787 268 1524 Fragments arranged by size: 1524 1410 795 787 597 393 347 268 EcoO109I rG'GnC_Cy Cuts at: 2387 2890 3175 2387 Size: 503 285 5333 Fragments arranged by size: 5333 503 285 EcoRI G'AATT_C Cuts at: 3430 3808 3430 Size: 378 5743 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3268 3268 Size: 6121 FseI GG_CCGG'CC Cuts at: 2232 2232 Size: 6121 FspI TGC'GCA Cuts at: 2358 4627 2358 Size: 2269 3852 HaeII r_GCGC'y Cuts at: 1678 2280 2327 2349 2564 2644 2800 2865 2904 Size: 602 47 22 215 80 156 65 39 Cuts at: 2904 2919 3042 3104 3330 5268 1678 Size: 15 123 62 226 1938 2531 Fragments arranged by size: 2531 1938 602 226 215 156 123 80 65 62 47 39 22 15 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4762 4794 4836 4868 1373 Size: 32 135 32 3190 32 42 32 2626 Fragments arranged by size: 3190 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3465 3711 669 Size: 988 281 1527 246 3079 Fragments arranged by size: 3079 1527 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6121 HpaI GTT'AAC Cuts at: 3711 3711 Size: 6121 KasI G'GCGC_C Cuts at: 2276 2345 2560 2861 2900 3038 2276 Size: 69 215 301 39 138 5359 Fragments arranged by size: 5359 301 215 138 69 39 KpnI G_GTAC'C Cuts at: 3446 3446 Size: 6121 MfeI C'AATT_G Cuts at: 3720 3720 Size: 6121 MluI A'CGCG_T Cuts at: 5997 5997 Size: 6121 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2466 5235 5419 1305 Size: 1161 2769 184 2007 Fragments arranged by size: 2769 2007 1161 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2549 10 Size: 54 1425 343 717 3582 Fragments arranged by size: 3582 1425 717 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2144 2150 2543 2937 4079 4438 4597 Size: 648 978 6 393 394 1142 359 159 Cuts at: 4597 518 Size: 2042 Fragments arranged by size: 2042 1142 978 648 394 393 359 159 6 MspA1I CmG'CkG Cuts at: 1606 2859 3160 3537 4214 5362 5607 1606 Size: 1253 301 377 677 1148 245 2120 Fragments arranged by size: 2120 1253 1148 677 377 301 245 NaeI GCC'GGC Cuts at: 2230 2254 2302 2827 2976 3375 2230 Size: 24 48 525 149 399 4976 Fragments arranged by size: 4976 525 399 149 48 24 NarI GG'CG_CC Cuts at: 2277 2346 2561 2862 2901 3039 2277 Size: 69 215 301 39 138 5359 Fragments arranged by size: 5359 301 215 138 69 39 NcoI C'CATG_G Cuts at: 513 1065 2313 2349 2424 2544 513 Size: 552 1248 36 75 120 4090 Fragments arranged by size: 4090 1248 552 120 75 36 NdeI CA'TA_TG Cuts at: 387 387 Size: 6121 NgoMIV G'CCGG_C Cuts at: 2228 2252 2300 2825 2974 3373 2228 Size: 24 48 525 149 399 4976 Fragments arranged by size: 4976 525 399 149 48 24 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6121 NotI GC'GGCC_GC Cuts at: 2397 3568 2397 Size: 1171 4950 NspI r_CATG'y Cuts at: 1132 1229 2203 2260 2764 3479 5024 1132 Size: 97 974 57 504 715 1545 2229 Fragments arranged by size: 2229 1545 974 715 504 97 57 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6121 PciI A'CATG_T Cuts at: 2199 2760 5020 2199 Size: 561 2260 3300 Fragments arranged by size: 3300 2260 561 PflMI CCAn_nnn'nTGG Cuts at: 1108 2046 3007 1108 Size: 938 961 4222 Fragments arranged by size: 4222 961 938 PmeI GTTT'AAAC Cuts at: 3425 3425 Size: 6121 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4190 4222 5703 5735 4190 Size: 32 1481 32 4576 Fragments arranged by size: 4576 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2890 3175 2890 Size: 285 5836 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6121 PsiI TTA'TAA Cuts at: 3691 3691 Size: 6121 PspOMI G'GGCC_C Cuts at: 2386 2984 3092 2386 Size: 598 108 5415 Fragments arranged by size: 5415 598 108 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2042 2074 2762 2794 2042 Size: 32 688 32 5369 Fragments arranged by size: 5369 688 32 32 PstI C_TGCA'G Cuts at: 830 3473 830 Size: 2643 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6121 PvuII CAG'CTG Cuts at: 3160 3160 Size: 6121 SalI G'TCGA_C Cuts at: 1936 3463 1936 Size: 1527 4594 SanDI GG'GwC_CC Cuts at: 3175 3175 Size: 6121 SbfI CC_TGCA'GG Cuts at: 3473 3473 Size: 6121 ScaI AGT'ACT Cuts at: 4369 6081 4369 Size: 1712 4409 SfcI C'TryA_G Cuts at: 826 1045 3469 4604 5285 5476 826 Size: 219 2424 1135 681 191 1471 Fragments arranged by size: 2424 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2278 2347 2562 2863 2902 3040 2278 Size: 69 215 301 39 138 5359 Fragments arranged by size: 5359 301 215 138 69 39 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6121 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6121 SmaI CCC'GGG Cuts at: 1872 2217 2983 3097 3448 1872 Size: 345 766 114 351 4545 Fragments arranged by size: 4545 766 351 345 114 SmlI C'TyrA_G Cuts at: 820 1017 1943 4225 5126 5388 820 Size: 197 926 2282 901 262 1553 Fragments arranged by size: 2282 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3912 493 Size: 3419 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6121 SphI G_CATG'C Cuts at: 1132 2260 3479 1132 Size: 1128 1219 3774 Fragments arranged by size: 3774 1219 1128 SspI AAT'ATT Cuts at: 5 52 4045 5 Size: 47 3993 2081 Fragments arranged by size: 3993 2081 47 StuI AGG'CCT Cuts at: 1517 2078 2115 1517 Size: 561 37 5523 Fragments arranged by size: 5523 561 37 StyI C'CwwG_G Cuts at: 513 1065 2221 2313 2349 2424 2544 2865 3385 Size: 552 1156 92 36 75 120 321 520 Cuts at: 3385 3560 513 Size: 175 3074 Fragments arranged by size: 3074 1156 552 520 321 175 120 92 75 36 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2342 4148 4165 4318 4503 4948 325 Size: 1255 762 1806 17 153 185 445 1498 Fragments arranged by size: 1806 1498 1255 762 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4367 6079 96 Size: 274 80 33 51 1443 2390 1712 138 Fragments arranged by size: 2390 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2654 2686 2735 2767 3188 3220 6081 6113 2654 Size: 32 49 32 421 32 2861 32 2662 Fragments arranged by size: 2861 2662 421 49 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6121 XbaI T'CTAG_A Cuts at: 3457 3457 Size: 6121 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2380 2380 Size: 6121 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6121 XmaI C'CCGG_G Cuts at: 1870 2215 2981 3095 3446 1870 Size: 345 766 114 351 4545 Fragments arranged by size: 4545 766 351 345 114 XmnI GAAnn'nnTTC Cuts at: 2798 4250 2798 Size: 1452 4669 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3929 276 Size: 53 83 186 936 2395 2468 Fragments arranged by size: 2468 2395 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI BaeI BfrBI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FspAI NruI NsiI PacI PfoI PmlI PspXI RsrII SacI SacII SapI SexAI SfiI SrfI SwaI