(Circular) (Six-base) MAPSORT of: pFN21AB8363.seq Check: 9395 from: 1 to: 6214 pFN21AB8363 6214 bp With 182 enzymes: SgfI * February 27, 2009 14:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4024 278 Size: 53 83 186 936 2488 2468 Fragments arranged by size: 2488 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3557 1937 Size: 1620 4594 Acc65I G'GTAC_C Cuts at: 3099 3535 3099 Size: 436 5778 AclI AA'CG_TT Cuts at: 1563 4341 4714 1563 Size: 2778 373 3063 Fragments arranged by size: 3063 2778 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2079 4277 5661 5929 1632 Size: 447 2198 1384 268 1917 Fragments arranged by size: 2198 1917 1384 447 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6017 AflIII A'CryG_T Cuts at: 1203 2562 3511 5113 6090 1203 Size: 1359 949 1602 977 1327 Fragments arranged by size: 1602 1359 1327 977 949 AgeI A'CCGG_T Cuts at: 5045 5045 Size: 6214 AhdI GACnn_n'nnGTC Cuts at: 2935 4943 2935 Size: 2008 4206 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6214 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2399 1184 Size: 1215 4999 AlwNI CAG_nnn'CTG Cuts at: 2153 2223 2927 3957 5529 2153 Size: 70 704 1030 1572 2838 Fragments arranged by size: 2838 1572 1030 704 70 ApaI G_GGCC'C Cuts at: 3306 3306 Size: 6214 ApaLI G'TGCA_C Cuts at: 4271 5427 4271 Size: 1156 5058 ApoI r'AATT_y Cuts at: 1493 2252 3239 3253 3523 3749 3901 6010 1493 Size: 759 987 14 270 226 152 2109 1697 Fragments arranged by size: 2109 1697 987 759 270 226 152 14 AseI AT'TA_AT Cuts at: 160 1032 4768 160 Size: 872 3736 1606 Fragments arranged by size: 3736 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6214 AvaI C'yCGr_G Cuts at: 1870 1943 3215 3539 1870 Size: 73 1272 324 4545 Fragments arranged by size: 4545 1272 324 73 BamHI G'GATC_C Cuts at: 3544 3911 3544 Size: 367 5847 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3099 3535 4990 618 Size: 325 217 627 1312 436 1455 1842 Fragments arranged by size: 1842 1455 1312 627 436 325 217 BanII G_rGCy'C Cuts at: 1391 2424 3306 3533 1391 Size: 1033 882 227 4072 Fragments arranged by size: 4072 1033 882 227 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3392 928 Size: 971 1493 3750 Fragments arranged by size: 3750 1493 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4405 4439 1903 Size: 34 2468 34 3678 Fragments arranged by size: 3678 2468 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4106 5316 4106 Size: 1210 5004 BclI T'GATC_A Cuts at: 1548 1548 Size: 6214 BfrBI ATG'CAT Cuts at: 2386 2672 2386 Size: 286 5928 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4825 136 Size: 107 122 71 631 605 3153 1525 Fragments arranged by size: 3153 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6209 6209 Size: 6214 BlpI GC'TnA_GC Cuts at: 3631 3631 Size: 6214 Bme1580I G_kGCm'C Cuts at: 1790 2135 3306 4275 5431 1790 Size: 345 1171 969 1156 2573 Fragments arranged by size: 2573 1171 1156 969 345 BmgBI CAC'GTC Cuts at: 1318 2563 1318 Size: 1245 4969 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3158 3945 4903 Size: 416 53 83 399 24 1741 787 958 Cuts at: 4903 442 Size: 1753 Fragments arranged by size: 1753 1741 958 787 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6214 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2295 4874 1239 Size: 1056 2579 2579 Fragments arranged by size: 2579 2579 1056 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2290 2635 4339 5204 5502 2290 Size: 345 1704 865 298 3002 Fragments arranged by size: 3002 1704 865 345 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4877 882 Size: 617 3378 2219 Fragments arranged by size: 3378 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3382 4005 493 Size: 711 2178 623 2702 Fragments arranged by size: 2702 2178 711 623 BsaBI GATnn'nnATC Cuts at: 2210 6208 2210 Size: 3998 2216 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4021 4403 5038 275 Size: 53 83 186 936 2488 382 635 1451 Fragments arranged by size: 2488 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4647 5045 5319 5466 5877 4647 Size: 398 274 147 411 4984 Fragments arranged by size: 4984 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5989 Fragments arranged by size: 5989 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2084 2546 2675 1186 Size: 898 462 129 4725 Fragments arranged by size: 4725 898 462 129 BseYI C'CCAG_C Cuts at: 5417 5913 5417 Size: 496 5718 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3336 1163 Size: 573 1600 4041 Fragments arranged by size: 4041 1600 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3664 4425 5453 1540 Size: 275 186 1663 761 1028 2301 Fragments arranged by size: 2301 1663 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2424 4275 4360 5431 2424 Size: 1851 85 1071 3207 Fragments arranged by size: 3207 1851 1071 85 BsmI GAATG_Cn' Cuts at: 3725 3818 3725 Size: 93 6121 BsmBI CGTCTCn'nnnn_ Cuts at: 3364 3364 Size: 6214 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2135 2424 3306 3533 4275 4360 5431 Size: 399 345 289 882 227 742 85 1071 Cuts at: 5431 1391 Size: 2174 Fragments arranged by size: 2174 1071 882 742 399 345 289 227 85 BspEI T'CCGG_A Cuts at: 5877 5877 Size: 6214 BspHI T'CATG_A Cuts at: 4103 5833 4103 Size: 1730 4484 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3569 844 Size: 2725 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4101 1394 Size: 429 2278 3507 Fragments arranged by size: 3507 2278 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2967 3389 4709 4883 65 Size: 1183 1719 422 1320 174 1396 Fragments arranged by size: 1719 1396 1320 1183 422 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4858 5045 1700 Size: 110 3048 187 2869 Fragments arranged by size: 3048 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6214 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6214 BssSI C'ACGA_G Cuts at: 4274 5286 4274 Size: 1012 5202 BstAPI GCAn_nnn'nTGC Cuts at: 794 2223 794 Size: 1429 4785 BstBI TT'CG_AA Cuts at: 5018 5018 Size: 6214 BstEII G'GTnAC_C Cuts at: 1184 2536 2870 1184 Size: 1352 334 4528 Fragments arranged by size: 4528 1352 334 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2424 2011 Size: 413 5801 BstYI r'GATC_y Cuts at: 1972 2268 2619 3544 3911 4296 4313 5765 5851 Size: 296 351 925 367 385 17 1452 86 Cuts at: 5851 5863 6209 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 925 385 367 351 346 296 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2188 3307 3333 513 Size: 552 939 184 1119 26 3394 Fragments arranged by size: 3394 1119 939 552 184 26 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3313 487 Size: 683 56 2087 3388 Fragments arranged by size: 3388 2087 683 56 BtsI GCAGTG_nn' Cuts at: 2224 2378 3730 4524 4544 2224 Size: 154 1352 794 20 3894 Fragments arranged by size: 3894 1352 794 154 20 ClaI AT'CG_AT Cuts at: 1554 3153 1554 Size: 1599 4615 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6179 DraI TTT'AAA Cuts at: 3518 3518 Size: 6214 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5221 809 Size: 507 3905 1802 Fragments arranged by size: 3905 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3661 4550 6179 Size: 54 1425 50 275 18 1831 889 1629 Cuts at: 6179 8 Size: 43 Fragments arranged by size: 1831 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3661 1537 Size: 275 1849 4090 Fragments arranged by size: 4090 1849 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2615 2805 2985 4144 1353 Size: 1262 190 180 1159 3423 Fragments arranged by size: 3423 1262 1159 190 180 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4801 5175 5321 387 Size: 1064 138 3212 374 146 1280 Fragments arranged by size: 3212 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2422 2422 Size: 6214 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2079 2295 4277 4874 5661 5929 1239 Size: 393 447 216 1982 597 787 268 1524 Fragments arranged by size: 1982 1524 787 597 447 393 268 216 EcoO109I rG'GnC_Cy Cuts at: 2150 2465 3303 2150 Size: 315 838 5061 Fragments arranged by size: 5061 838 315 EcoRI G'AATT_C Cuts at: 2252 3523 3901 2252 Size: 1271 378 4565 Fragments arranged by size: 4565 1271 378 FspI TGC'GCA Cuts at: 3128 4720 3128 Size: 1592 4622 HaeII r_GCGC'y Cuts at: 1678 5361 1678 Size: 3683 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2120 2152 2166 2198 2253 Size: 32 135 32 548 32 14 32 55 Cuts at: 2253 2285 2908 2921 2940 2953 4855 4887 4929 Size: 32 623 13 19 13 1902 32 42 Cuts at: 4929 4961 1373 Size: 32 2626 Fragments arranged by size: 2626 1902 623 548 135 55 42 32 32 32 32 32 32 32 19 14 13 13 HincII GTy'rAC Cuts at: 669 1657 1938 2568 3558 3804 669 Size: 988 281 630 990 246 3079 Fragments arranged by size: 3079 990 988 630 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6214 HpaI GTT'AAC Cuts at: 3804 3804 Size: 6214 KpnI G_GTAC'C Cuts at: 3103 3539 3103 Size: 436 5778 MfeI C'AATT_G Cuts at: 3813 3813 Size: 6214 MluI A'CGCG_T Cuts at: 6090 6090 Size: 6214 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2508 5328 5512 1305 Size: 1203 2820 184 2007 Fragments arranged by size: 2820 2007 1203 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4392 Fragments arranged by size: 4392 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2381 2982 3332 3338 3447 4172 4531 Size: 648 1215 601 350 6 109 725 359 Cuts at: 4531 4690 518 Size: 159 2042 Fragments arranged by size: 2042 1215 725 648 601 359 350 159 109 6 MspA1I CmG'CkG Cuts at: 1606 3630 4307 5455 5700 1606 Size: 2024 677 1148 245 2120 Fragments arranged by size: 2120 2024 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3333 513 Size: 552 939 1329 3394 Fragments arranged by size: 3394 1329 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6214 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6214 NotI GC'GGCC_GC Cuts at: 3661 3661 Size: 6214 NsiI A_TGCA'T Cuts at: 2388 2674 2388 Size: 286 5928 NspI r_CATG'y Cuts at: 1132 1229 3097 3466 3572 5117 1132 Size: 97 1868 369 106 1545 2229 Fragments arranged by size: 2229 1868 1545 369 106 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6214 PciI A'CATG_T Cuts at: 5113 5113 Size: 6214 PflMI CCAn_nnn'nTGG Cuts at: 1108 2153 1108 Size: 1045 5169 PmeI GTTT'AAAC Cuts at: 3518 3518 Size: 6214 PmlI CAC'GTG Cuts at: 3382 3382 Size: 6214 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4283 4315 5796 5828 4283 Size: 32 1481 32 4669 Fragments arranged by size: 4669 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2150 2150 Size: 6214 PshAI GACnn'nnGTC Cuts at: 1613 3501 1613 Size: 1888 4326 PsiI TTA'TAA Cuts at: 2037 3784 2037 Size: 1747 4467 PspOMI G'GGCC_C Cuts at: 3302 3302 Size: 6214 PspXI vC'TCGA_Gb Cuts at: 3215 3215 Size: 6214 PstI C_TGCA'G Cuts at: 830 2373 3566 830 Size: 1543 1193 3478 Fragments arranged by size: 3478 1543 1193 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6214 SacI G_AGCT'C Cuts at: 2424 2424 Size: 6214 SalI G'TCGA_C Cuts at: 1936 3556 1936 Size: 1620 4594 SapI GCTCTTCn'nnn_ Cuts at: 2615 2615 Size: 6214 SbfI CC_TGCA'GG Cuts at: 3566 3566 Size: 6214 ScaI AGT'ACT Cuts at: 4462 6174 4462 Size: 1712 4502 SexAI A'CCwGG_T Cuts at: 2146 2146 Size: 6214 SfcI C'TryA_G Cuts at: 826 1045 2369 3562 4697 5378 5569 826 Size: 219 1324 1193 1135 681 191 1471 Fragments arranged by size: 1471 1324 1193 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6214 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6214 SmaI CCC'GGG Cuts at: 1872 3541 1872 Size: 1669 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2305 2614 3215 4318 5219 5481 Size: 197 926 362 309 601 1103 901 262 Cuts at: 5481 820 Size: 1553 Fragments arranged by size: 1553 1103 926 901 601 362 309 262 197 SnaBI TAC'GTA Cuts at: 493 4005 493 Size: 3512 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6214 SphI G_CATG'C Cuts at: 1132 3097 3572 1132 Size: 1965 475 3774 Fragments arranged by size: 3774 1965 475 SspI AAT'ATT Cuts at: 5 52 3196 4138 5 Size: 47 3144 942 2081 Fragments arranged by size: 3144 2081 942 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6214 StyI C'CwwG_G Cuts at: 513 1065 2004 2656 3333 3653 513 Size: 552 939 652 677 320 3074 Fragments arranged by size: 3074 939 677 652 552 320 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3459 4241 4258 4411 4596 5041 325 Size: 1255 1879 782 17 153 185 445 1498 Fragments arranged by size: 1879 1498 1255 782 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3324 4460 6172 Size: 274 80 33 51 1443 1347 1136 1712 Cuts at: 6172 96 Size: 138 Fragments arranged by size: 1712 1443 1347 1136 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2203 2235 3419 3451 6174 6206 2203 Size: 32 1184 32 2723 32 2211 Fragments arranged by size: 2723 2211 1184 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3119 1316 Size: 1803 4411 XbaI T'CTAG_A Cuts at: 3550 3550 Size: 6214 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3020 3020 Size: 6214 XhoI C'TCGA_G Cuts at: 1943 3215 1943 Size: 1272 4942 XmaI C'CCGG_G Cuts at: 1870 3539 1870 Size: 1669 4545 XmnI GAAnn'nnTTC Cuts at: 4343 4343 Size: 6214 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4022 276 Size: 53 83 186 936 2488 2468 Fragments arranged by size: 2488 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PsrI PvuII RsrII SacII SanDI SfiI SfoI SrfI SwaI