(Circular) (Six-base) MAPSORT of: pFN21AB8347.seq Check: 9606 from: 1 to: 5839 pFN21AB8347 5839 bp With 182 enzymes: SgfI * February 27, 2009 14:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2617 2617 Size: 5839 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3649 278 Size: 53 83 186 936 2113 2468 Fragments arranged by size: 2468 2113 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2895 3182 1937 Size: 958 287 4594 Fragments arranged by size: 4594 958 287 Acc65I G'GTAC_C Cuts at: 3160 3160 Size: 5839 AclI AA'CG_TT Cuts at: 1563 3966 4339 1563 Size: 2403 373 3063 Fragments arranged by size: 3063 2403 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2228 2359 3902 5286 5554 1632 Size: 596 131 1543 1384 268 1917 Fragments arranged by size: 1917 1543 1384 596 268 131 AfeI AGC'GCT Cuts at: 3008 3008 Size: 5839 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5642 AflIII A'CryG_T Cuts at: 1203 4738 5715 1203 Size: 3535 977 1327 Fragments arranged by size: 3535 1327 977 AgeI A'CCGG_T Cuts at: 4670 4670 Size: 5839 AhdI GACnn_n'nnGTC Cuts at: 4568 4568 Size: 5839 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5839 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5839 AlwNI CAG_nnn'CTG Cuts at: 2163 2275 2308 2953 3080 3582 5154 2163 Size: 112 33 645 127 502 1572 2848 Fragments arranged by size: 2848 1572 645 502 127 112 33 ApaLI G'TGCA_C Cuts at: 2613 3896 5052 2613 Size: 1283 1156 3400 Fragments arranged by size: 3400 1283 1156 ApoI r'AATT_y Cuts at: 1493 3148 3374 3526 5635 1493 Size: 1655 226 152 2109 1697 Fragments arranged by size: 2109 1697 1655 226 152 AseI AT'TA_AT Cuts at: 160 1032 4393 160 Size: 872 3361 1606 Fragments arranged by size: 3361 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5839 AvaI C'yCGr_G Cuts at: 1870 1943 2109 2402 2694 3164 1870 Size: 73 166 293 292 470 4545 Fragments arranged by size: 4545 470 293 292 166 73 BamHI G'GATC_C Cuts at: 3169 3536 3169 Size: 367 5472 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3160 4615 618 Size: 325 217 627 1373 1455 1842 Fragments arranged by size: 1842 1455 1373 627 325 217 BanII G_rGCy'C Cuts at: 1391 3158 1391 Size: 1767 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2058 2414 928 Size: 971 159 356 4353 Fragments arranged by size: 4353 971 356 159 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4030 4064 1903 Size: 34 2093 34 3678 Fragments arranged by size: 3678 2093 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3731 4941 3731 Size: 1210 4629 BclI T'GATC_A Cuts at: 1548 1548 Size: 5839 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2874 3053 4450 Size: 107 122 71 631 605 1202 179 1397 Cuts at: 4450 136 Size: 1525 Fragments arranged by size: 1525 1397 1202 631 605 179 122 107 71 BglII A'GATC_T Cuts at: 5834 5834 Size: 5839 BlpI GC'TnA_GC Cuts at: 2360 3256 2360 Size: 896 4943 Bme1580I G_kGCm'C Cuts at: 1790 2617 3900 5056 1790 Size: 827 1283 1156 2573 Fragments arranged by size: 2573 1283 1156 827 BmgBI CAC'GTC Cuts at: 1318 2944 1318 Size: 1626 4213 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3570 4528 442 Size: 416 53 83 399 24 2153 958 1753 Fragments arranged by size: 2153 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5839 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2437 2437 Size: 5839 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2237 2267 2330 2684 2903 4499 1239 Size: 998 30 63 354 219 1596 2579 Fragments arranged by size: 2579 1596 998 354 219 63 30 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3964 4829 5127 3964 Size: 865 298 4676 Fragments arranged by size: 4676 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4502 882 Size: 617 3003 2219 Fragments arranged by size: 3003 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3630 493 Size: 711 2426 2702 Fragments arranged by size: 2702 2426 711 BsaBI GATnn'nnATC Cuts at: 5833 5833 Size: 5839 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2961 3646 4028 4663 Size: 53 83 186 936 1428 685 382 635 Cuts at: 4663 275 Size: 1451 Fragments arranged by size: 1451 1428 936 685 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4272 4670 4944 5091 5502 4272 Size: 398 274 147 411 4609 Fragments arranged by size: 4609 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5614 Fragments arranged by size: 5614 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2300 2327 2429 2441 2570 2618 2855 2858 Size: 1114 27 102 12 129 48 237 3 Cuts at: 2858 2861 3083 1186 Size: 3 222 3942 Fragments arranged by size: 3942 1114 237 222 129 102 48 27 12 3 3 BseYI C'CCAG_C Cuts at: 2295 2505 5042 5538 2295 Size: 210 2537 496 2596 Fragments arranged by size: 2596 2537 496 210 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2966 1163 Size: 573 1230 4036 Fragments arranged by size: 4036 1230 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2740 3289 4050 5078 1540 Size: 275 186 739 549 761 1028 2301 Fragments arranged by size: 2301 1028 761 739 549 275 186 BsiHKAI G_wGCw'C Cuts at: 2617 3900 3985 5056 2617 Size: 1283 85 1071 3400 Fragments arranged by size: 3400 1283 1071 85 BsmI GAATG_Cn' Cuts at: 3350 3443 3350 Size: 93 5746 BsmBI CGTCTCn'nnnn_ Cuts at: 2051 2051 Size: 5839 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2617 3158 3900 3985 5056 1391 Size: 399 827 541 742 85 1071 2174 Fragments arranged by size: 2174 1071 827 742 541 399 85 BspEI T'CCGG_A Cuts at: 5502 5502 Size: 5839 BspHI T'CATG_A Cuts at: 3728 5458 3728 Size: 1730 4109 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2617 3065 3194 844 Size: 1773 448 129 3489 Fragments arranged by size: 3489 1773 448 129 BsrBI CCG'CTC Cuts at: 1394 1823 2290 3726 1394 Size: 429 467 1436 3507 Fragments arranged by size: 3507 1436 467 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4334 4508 65 Size: 1183 3086 174 1396 Fragments arranged by size: 3086 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4483 4670 1700 Size: 110 2673 187 2869 Fragments arranged by size: 2869 2673 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5839 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5839 BssSI C'ACGA_G Cuts at: 3899 4911 3899 Size: 1012 4827 BstAPI GCAn_nnn'nTGC Cuts at: 794 2953 794 Size: 2159 3680 BstBI TT'CG_AA Cuts at: 4643 4643 Size: 5839 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5839 BstXI CCAn_nnnn'nTGG Cuts at: 2572 2572 Size: 5839 BstYI r'GATC_y Cuts at: 1972 3169 3536 3921 3938 5390 5476 5488 5834 Size: 1197 367 385 17 1452 86 12 346 Cuts at: 5834 1972 Size: 1977 Fragments arranged by size: 1977 1452 1197 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2910 2910 Size: 5839 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4348 Fragments arranged by size: 4348 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3132 487 Size: 683 56 1906 3194 Fragments arranged by size: 3194 1906 683 56 BtsI GCAGTG_nn' Cuts at: 3355 4149 4169 3355 Size: 794 20 5025 Fragments arranged by size: 5025 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5839 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5804 DraI TTT'AAA Cuts at: 3143 3143 Size: 5839 DraIII CAC_nnn'GTG Cuts at: 2548 2548 Size: 5839 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4846 809 Size: 507 3530 1802 Fragments arranged by size: 3530 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2079 2187 2223 Size: 54 1425 50 275 18 249 108 36 Cuts at: 2223 2670 3286 4175 5804 8 Size: 447 616 889 1629 43 Fragments arranged by size: 1629 1425 889 616 447 275 249 108 54 50 43 36 18 EagI C'GGCC_G Cuts at: 1537 1812 3286 1537 Size: 275 1474 4090 Fragments arranged by size: 4090 1474 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2093 2327 3769 1353 Size: 740 234 1442 3423 Fragments arranged by size: 3423 1442 740 234 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4426 4800 4946 387 Size: 1064 138 2837 374 146 1280 Fragments arranged by size: 2837 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2228 2237 2267 2330 2359 2684 2903 Size: 393 596 9 30 63 29 325 219 Cuts at: 2903 3902 4499 5286 5554 1239 Size: 999 597 787 268 1524 Fragments arranged by size: 1524 999 787 597 596 393 325 268 219 63 30 29 9 EcoNI CCTnn'n_nnAGG Cuts at: 2909 2909 Size: 5839 EcoO109I rG'GnC_Cy Cuts at: 3031 3077 3085 3031 Size: 46 8 5785 Fragments arranged by size: 5785 46 8 EcoRI G'AATT_C Cuts at: 3148 3526 3148 Size: 378 5461 FspI TGC'GCA Cuts at: 2279 2321 2813 4345 2279 Size: 42 492 1532 3773 Fragments arranged by size: 3773 1532 492 42 HaeII r_GCGC'y Cuts at: 1678 2254 3010 4986 1678 Size: 576 756 1976 2531 Fragments arranged by size: 2531 1976 756 576 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2722 2754 4480 4512 4554 Size: 32 135 32 1150 32 1726 32 42 Cuts at: 4554 4586 1373 Size: 32 2626 Fragments arranged by size: 2626 1726 1150 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3183 3429 669 Size: 988 281 1245 246 3079 Fragments arranged by size: 3079 1245 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5839 HpaI GTT'AAC Cuts at: 3429 3429 Size: 5839 KpnI G_GTAC'C Cuts at: 3164 3164 Size: 5839 MfeI C'AATT_G Cuts at: 3438 3438 Size: 5839 MluI A'CGCG_T Cuts at: 5715 5715 Size: 5839 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4953 5137 1305 Size: 3648 184 2007 Fragments arranged by size: 3648 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2672 10 Size: 54 1425 343 840 3177 Fragments arranged by size: 3177 1425 840 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2036 3797 4156 4315 518 Size: 648 870 1761 359 159 2042 Fragments arranged by size: 2042 1761 870 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2094 2275 2505 3255 3932 5080 5325 1606 Size: 488 181 230 750 677 1148 245 2120 Fragments arranged by size: 2120 1148 750 677 488 245 230 181 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4348 Fragments arranged by size: 4348 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5839 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5839 NotI GC'GGCC_GC Cuts at: 3286 3286 Size: 5839 NspI r_CATG'y Cuts at: 1132 1229 2341 2497 3004 3197 4742 1132 Size: 97 1112 156 507 193 1545 2229 Fragments arranged by size: 2229 1545 1112 507 193 156 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5839 PciI A'CATG_T Cuts at: 4738 4738 Size: 5839 PflMI CCAn_nnn'nTGG Cuts at: 1108 2749 1108 Size: 1641 4198 PfoI T'CCnGG_A Cuts at: 2661 2661 Size: 5839 PmeI GTTT'AAAC Cuts at: 3143 3143 Size: 5839 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3908 3940 5421 5453 3908 Size: 32 1481 32 4294 Fragments arranged by size: 4294 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3031 3077 3085 3031 Size: 46 8 5785 Fragments arranged by size: 5785 46 8 PshAI GACnn'nnGTC Cuts at: 1613 2645 1613 Size: 1032 4807 PsiI TTA'TAA Cuts at: 3409 3409 Size: 5839 PstI C_TGCA'G Cuts at: 830 2958 3191 830 Size: 2128 233 3478 Fragments arranged by size: 3478 2128 233 PvuI CG_AT'CG Cuts at: 2001 2740 2001 Size: 739 5100 PvuII CAG'CTG Cuts at: 2275 2275 Size: 5839 SalI G'TCGA_C Cuts at: 1936 3181 1936 Size: 1245 4594 SanDI GG'GwC_CC Cuts at: 3031 3085 3031 Size: 54 5785 SapI GCTCTTCn'nnn_ Cuts at: 2327 2327 Size: 5839 SbfI CC_TGCA'GG Cuts at: 3191 3191 Size: 5839 ScaI AGT'ACT Cuts at: 4087 5799 4087 Size: 1712 4127 SexAI A'CCwGG_T Cuts at: 2544 2571 2544 Size: 27 5812 SfcI C'TryA_G Cuts at: 826 1045 2014 2954 3187 4322 5003 5194 826 Size: 219 969 940 233 1135 681 191 1471 Fragments arranged by size: 1471 1135 969 940 681 233 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2874 3053 2874 Size: 179 5660 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5839 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5839 SmaI CCC'GGG Cuts at: 1872 2111 2696 3166 1872 Size: 239 585 470 4545 Fragments arranged by size: 4545 585 470 239 SmlI C'TyrA_G Cuts at: 820 1017 1943 3943 4844 5106 820 Size: 197 926 2000 901 262 1553 Fragments arranged by size: 2000 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3630 493 Size: 3137 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5839 SphI G_CATG'C Cuts at: 1132 2341 2497 3197 1132 Size: 1209 156 700 3774 Fragments arranged by size: 3774 1209 700 156 SspI AAT'ATT Cuts at: 5 52 3763 5 Size: 47 3711 2081 Fragments arranged by size: 3711 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5839 StyI C'CwwG_G Cuts at: 513 1065 2004 3057 3278 513 Size: 552 939 1053 221 3074 Fragments arranged by size: 3074 1053 939 552 221 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3866 3883 4036 4221 4666 325 Size: 1255 2286 17 153 185 445 1498 Fragments arranged by size: 2286 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2745 4085 5797 Size: 274 80 33 51 1443 768 1340 1712 Cuts at: 5797 96 Size: 138 Fragments arranged by size: 1712 1443 1340 768 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5799 5831 5799 Size: 32 5807 Tth111I GACn'n_nGTC Cuts at: 1316 2537 2885 1316 Size: 1221 348 4270 Fragments arranged by size: 4270 1221 348 XbaI T'CTAG_A Cuts at: 3175 3175 Size: 5839 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2971 2971 Size: 5839 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5839 XmaI C'CCGG_G Cuts at: 1870 2109 2694 3164 1870 Size: 239 585 470 4545 Fragments arranged by size: 4545 585 470 239 XmnI GAAnn'nnTTC Cuts at: 2927 3102 3968 2927 Size: 175 866 4798 Fragments arranged by size: 4798 866 175 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3647 276 Size: 53 83 186 936 2113 2468 Fragments arranged by size: 2468 2113 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstZ17I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SfoI SrfI SwaI