(Circular) (Six-base) MAPSORT of: pFN21AB8342.seq Check: 895 from: 1 to: 5749 pFN21AB8342 5749 bp With 182 enzymes: SgfI * October 20, 2009 10:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3559 278 Size: 53 83 186 936 2023 2468 Fragments arranged by size: 2468 2023 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3092 1937 Size: 1155 4594 Acc65I G'GTAC_C Cuts at: 3070 3070 Size: 5749 AclI AA'CG_TT Cuts at: 1563 3876 4249 1563 Size: 2313 373 3063 Fragments arranged by size: 3063 2313 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3812 5196 5464 1632 Size: 2180 1384 268 1917 Fragments arranged by size: 2180 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5552 AflIII A'CryG_T Cuts at: 1203 2133 2622 4648 5625 1203 Size: 930 489 2026 977 1327 Fragments arranged by size: 2026 1327 977 930 489 AgeI A'CCGG_T Cuts at: 4580 4580 Size: 5749 AhdI GACnn_n'nnGTC Cuts at: 4478 4478 Size: 5749 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5749 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5749 AlwNI CAG_nnn'CTG Cuts at: 3492 5064 3492 Size: 1572 4177 ApaI G_GGCC'C Cuts at: 2325 2325 Size: 5749 ApaLI G'TGCA_C Cuts at: 3806 4962 3806 Size: 1156 4593 ApoI r'AATT_y Cuts at: 1493 3058 3284 3436 5545 1493 Size: 1565 226 152 2109 1697 Fragments arranged by size: 2109 1697 1565 226 152 AseI AT'TA_AT Cuts at: 160 1032 4303 160 Size: 872 3271 1606 Fragments arranged by size: 3271 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5749 AvaI C'yCGr_G Cuts at: 1870 1943 2543 3074 1870 Size: 73 600 531 4545 Fragments arranged by size: 4545 600 531 73 AvrII C'CTAG_G Cuts at: 2266 2266 Size: 5749 BamHI G'GATC_C Cuts at: 3079 3446 3079 Size: 367 5382 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2800 3070 4525 618 Size: 325 217 627 1013 270 1455 1842 Fragments arranged by size: 1842 1455 1013 627 325 270 217 BanII G_rGCy'C Cuts at: 1391 2325 2846 3068 1391 Size: 934 521 222 4072 Fragments arranged by size: 4072 934 521 222 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2308 2677 928 Size: 971 409 369 4000 Fragments arranged by size: 4000 971 409 369 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2056 2090 3940 3974 1903 Size: 34 119 34 1850 34 3678 Fragments arranged by size: 3678 1850 119 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2300 3641 4851 2300 Size: 1341 1210 3198 Fragments arranged by size: 3198 1341 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5749 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2816 4360 136 Size: 107 122 71 631 605 1144 1544 1525 Fragments arranged by size: 1544 1525 1144 631 605 122 107 71 BglII A'GATC_T Cuts at: 5744 5744 Size: 5749 BlpI GC'TnA_GC Cuts at: 2100 3166 2100 Size: 1066 4683 Bme1580I G_kGCm'C Cuts at: 1790 2040 2325 2805 3810 4966 1790 Size: 250 285 480 1005 1156 2573 Fragments arranged by size: 2573 1156 1005 480 285 250 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5749 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2996 3480 4438 Size: 416 53 83 399 24 1579 484 958 Cuts at: 4438 442 Size: 1753 Fragments arranged by size: 1753 1579 958 484 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5749 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4409 1239 Size: 3170 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2570 3874 4739 5037 2570 Size: 1304 865 298 3282 Fragments arranged by size: 3282 1304 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4412 882 Size: 617 2913 2219 Fragments arranged by size: 2913 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2035 2413 3540 493 Size: 711 831 378 1127 2702 Fragments arranged by size: 2702 1127 831 711 378 BsaBI GATnn'nnATC Cuts at: 2657 5743 2657 Size: 3086 2663 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3556 3938 4573 275 Size: 53 83 186 936 2023 382 635 1451 Fragments arranged by size: 2023 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2943 4182 4580 4854 5001 5412 2943 Size: 1239 398 274 147 411 3280 Fragments arranged by size: 3280 1239 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2828 2858 1178 Size: 30 165 30 1425 30 4069 Fragments arranged by size: 4069 1425 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5749 BseYI C'CCAG_C Cuts at: 2152 2709 2796 2875 4952 5448 2152 Size: 557 87 79 2077 496 2453 Fragments arranged by size: 2453 2077 557 496 87 79 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5176 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3048 3199 3960 4988 1540 Size: 275 186 1047 151 761 1028 2301 Fragments arranged by size: 2301 1047 1028 761 275 186 151 BsiHKAI G_wGCw'C Cuts at: 2561 3810 3895 4966 2561 Size: 1249 85 1071 3344 Fragments arranged by size: 3344 1249 1071 85 BsmI GAATG_Cn' Cuts at: 2819 3260 3353 2819 Size: 441 93 5215 Fragments arranged by size: 5215 441 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3020 3020 Size: 5749 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2040 2325 2561 2805 2846 3068 3810 Size: 399 250 285 236 244 41 222 742 Cuts at: 3810 3895 4966 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 285 250 244 236 222 85 41 BspEI T'CCGG_A Cuts at: 2943 5412 2943 Size: 2469 3280 BspHI T'CATG_A Cuts at: 2865 2925 3638 5368 2865 Size: 60 713 1730 3246 Fragments arranged by size: 3246 1730 713 60 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3104 844 Size: 2260 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2407 3636 1394 Size: 429 584 1229 3507 Fragments arranged by size: 3507 1229 584 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4244 4418 65 Size: 1183 2996 174 1396 Fragments arranged by size: 2996 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2522 4393 4580 1700 Size: 110 712 1871 187 2869 Fragments arranged by size: 2869 1871 712 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5749 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5749 BssSI C'ACGA_G Cuts at: 2560 3809 4821 2560 Size: 1249 1012 3488 Fragments arranged by size: 3488 1249 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5749 BstBI TT'CG_AA Cuts at: 4553 4553 Size: 5749 BstEII G'GTnAC_C Cuts at: 1184 2752 1184 Size: 1568 4181 BstYI r'GATC_y Cuts at: 1972 3079 3446 3831 3848 5300 5386 5398 5744 Size: 1107 367 385 17 1452 86 12 346 Cuts at: 5744 1972 Size: 1977 Fragments arranged by size: 1977 1452 1107 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2105 2244 513 Size: 552 1040 139 4018 Fragments arranged by size: 4018 1040 552 139 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2764 487 Size: 683 56 1538 3472 Fragments arranged by size: 3472 1538 683 56 BtsI GCAGTG_nn' Cuts at: 2561 3265 4059 4079 2561 Size: 704 794 20 4231 Fragments arranged by size: 4231 794 704 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5749 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5714 DraI TTT'AAA Cuts at: 3053 3053 Size: 5749 DraIII CAC_nnn'GTG Cuts at: 2990 2990 Size: 5749 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4756 809 Size: 507 3440 1802 Fragments arranged by size: 3440 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2124 2397 3045 Size: 54 1425 50 275 18 294 273 648 Cuts at: 3045 3196 4085 5714 8 Size: 151 889 1629 43 Fragments arranged by size: 1629 1425 889 648 294 275 273 151 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3045 3196 1537 Size: 275 1233 151 4090 Fragments arranged by size: 4090 1233 275 151 EarI CTCTTCn'nnn_ Cuts at: 1353 2261 3679 1353 Size: 908 1418 3423 Fragments arranged by size: 3423 1418 908 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4336 4710 4856 387 Size: 1064 138 2747 374 146 1280 Fragments arranged by size: 2747 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3812 4409 5196 5464 1239 Size: 393 2180 597 787 268 1524 Fragments arranged by size: 2180 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2021 2548 2021 Size: 527 5222 EcoRI G'AATT_C Cuts at: 3058 3436 3058 Size: 378 5371 FspI TGC'GCA Cuts at: 2210 4255 2210 Size: 2045 3704 HaeII r_GCGC'y Cuts at: 1678 2498 2898 4896 1678 Size: 820 400 1998 2531 Fragments arranged by size: 2531 1998 820 400 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2298 2330 2987 3019 4390 Size: 32 135 32 726 32 657 32 1371 Cuts at: 4390 4422 4464 4496 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1371 726 657 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2599 3093 3339 669 Size: 988 281 661 494 246 3079 Fragments arranged by size: 3079 988 661 494 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5749 HpaI GTT'AAC Cuts at: 3339 3339 Size: 5749 KpnI G_GTAC'C Cuts at: 3074 3074 Size: 5749 MfeI C'AATT_G Cuts at: 3348 3348 Size: 5749 MluI A'CGCG_T Cuts at: 5625 5625 Size: 5749 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2871 4863 5047 1305 Size: 1566 1992 184 2007 Fragments arranged by size: 2007 1992 1566 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2399 10 Size: 54 1425 343 567 3360 Fragments arranged by size: 3360 1425 567 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2621 2627 2648 2759 2771 2864 3707 Size: 648 1455 6 21 111 12 93 843 Cuts at: 3707 4066 4225 518 Size: 359 159 2042 Fragments arranged by size: 2042 1455 843 648 359 159 111 93 21 12 6 MspA1I CmG'CkG Cuts at: 1606 2705 3165 3842 4990 5235 1606 Size: 1099 460 677 1148 245 2120 Fragments arranged by size: 2120 1148 1099 677 460 245 NaeI GCC'GGC Cuts at: 2524 2524 Size: 5749 NcoI C'CATG_G Cuts at: 513 1065 2105 513 Size: 552 1040 4157 Fragments arranged by size: 4157 1040 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5749 NgoMIV G'CCGG_C Cuts at: 2522 2522 Size: 5749 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5749 NotI GC'GGCC_GC Cuts at: 3196 3196 Size: 5749 NspI r_CATG'y Cuts at: 1132 1229 2137 2626 2956 3107 4652 1132 Size: 97 908 489 330 151 1545 2229 Fragments arranged by size: 2229 1545 908 489 330 151 97 PasI CC'CwG_GG Cuts at: 1348 2359 1348 Size: 1011 4738 PciI A'CATG_T Cuts at: 2133 2622 4648 2133 Size: 489 2026 3234 Fragments arranged by size: 3234 2026 489 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5749 PmeI GTTT'AAAC Cuts at: 3053 3053 Size: 5749 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3818 3850 5331 5363 3818 Size: 32 1481 32 4204 Fragments arranged by size: 4204 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5749 PsiI TTA'TAA Cuts at: 3319 3319 Size: 5749 PspOMI G'GGCC_C Cuts at: 2321 2321 Size: 5749 PstI C_TGCA'G Cuts at: 830 2556 3101 830 Size: 1726 545 3478 Fragments arranged by size: 3478 1726 545 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5749 SalI G'TCGA_C Cuts at: 1936 3091 1936 Size: 1155 4594 SbfI CC_TGCA'GG Cuts at: 3101 3101 Size: 5749 ScaI AGT'ACT Cuts at: 2090 3997 5709 2090 Size: 1907 1712 2130 Fragments arranged by size: 2130 1907 1712 SfcI C'TryA_G Cuts at: 826 1045 2552 3097 4232 4913 5104 826 Size: 219 1507 545 1135 681 191 1471 Fragments arranged by size: 1507 1471 1135 681 545 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5749 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5749 SmaI CCC'GGG Cuts at: 1872 3076 1872 Size: 1204 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2585 3853 4754 5016 820 Size: 197 926 642 1268 901 262 1553 Fragments arranged by size: 1553 1268 926 901 642 262 197 SnaBI TAC'GTA Cuts at: 493 3540 493 Size: 3047 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5749 SphI G_CATG'C Cuts at: 1132 2956 3107 1132 Size: 1824 151 3774 Fragments arranged by size: 3774 1824 151 SspI AAT'ATT Cuts at: 5 52 3673 5 Size: 47 3621 2081 Fragments arranged by size: 3621 2081 47 StuI AGG'CCT Cuts at: 1517 3002 1517 Size: 1485 4264 StyI C'CwwG_G Cuts at: 513 1065 2105 2266 2385 3188 513 Size: 552 1040 161 119 803 3074 Fragments arranged by size: 3074 1040 803 552 161 119 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2788 2843 2849 2978 3776 3793 3946 Size: 1255 1208 55 6 129 798 17 153 Cuts at: 3946 4131 4576 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1208 798 445 185 153 129 55 17 6 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2088 3995 5707 Size: 274 80 33 51 1443 111 1907 1712 Cuts at: 5707 96 Size: 138 Fragments arranged by size: 1907 1712 1443 274 138 111 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2828 2860 5709 5741 2828 Size: 32 2849 32 2836 Fragments arranged by size: 2849 2836 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5749 XbaI T'CTAG_A Cuts at: 3085 3085 Size: 5749 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2523 2523 Size: 5749 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5749 XmaI C'CCGG_G Cuts at: 1870 3074 1870 Size: 1204 4545 XmnI GAAnn'nnTTC Cuts at: 3878 3878 Size: 5749 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3557 276 Size: 53 83 186 936 2023 2468 Fragments arranged by size: 2468 2023 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI