################################## # NO CUT ENZYMES ( 117 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AflIII AgeI AhdI ApaI AscI AseI AsnI Asp718I AspEI AvrII BalI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BspDI BspEI BspHI BspLU11I BsrDI Bst1107I BstBI BstZ17I ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco52I EcoNI EcoRI EcoRV EcoT22I FbaI FseI HgaI Hin1I HincII HindII HpaI KasI KpnI Ksp632I KspI MaeII MamI MluI MluNI MroI MscI MseI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI SwaI TfiI Tru9I Van91I XbaI XhoI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccI 412 GTAGAC GT^[AC][GT]AC AcsI 538 GAATTT [GA]^AATT[CT] Alw44I 319 GTGCAC G^TGCAC Aor51HI 1208 AGCGCT AGC^GCT ApaLI 319 GTGCAC G^TGCAC ApoI 538 GAATTT [GA]^AATT[CT] AspHI 319 GTGCAC G[AT]GC[AT]^C AspI 394 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 25 CCCGGG C^[CT]CG[GA]G AviII 56 TGCGCA TGC^GCA BamHI 484 GGATCC G^GATCC BbsI 391 GAAGAC GAAGAC(2/6) BfaI 1150 CTAG C^TAG BpuAI 391 GAAGAC GAAGAC(2/6) BseRI 217 GAGGAG GAGGAG(10/8) BsgI 1193 GTGCAG GTGCAG(16/14) BsiHKAI 319 GTGCAC G[AT]GC[AT]^C BsoBI 25 CCCGGG C^[CT]CG[GA]G Bsp1407I 797 TGTACA T^GTACA BsrFI 686 GCCGGC [GA]^CCGG[CT] BsrGI 797 TGTACA T^GTACA BssHII 829 GCGCGC G^CGCGC BssSI 325 CACGAG CACGAG(-5/-1) BstEII 186 GGTCACC G^GT[ACGT]ACC BstPI 186 GGTCACC G^GT[ACGT]ACC Cfr10I 686 GCCGGC [GA]^CCGG[CT] DsaI 192 CCATGG C^C[GA][CT]GG Eco47III 1208 AGCGCT AGC^GCT EcoO65I 186 GGTCACC G^GT[ACGT]ACC FspI 56 TGCGCA TGC^GCA HaeII 1208 AGCGCT [GA]GCGC^[CT] HindIII 871 AAGCTT A^AGCTT HinfI 382 GAGTC G^A[ACGT]TC MaeI 1150 CTAG C^TAG MfeI 1052 CAATTG C^AATTG MunI 1052 CAATTG C^AATTG NaeI 686 GCCGGC GCC^GGC NcoI 192 CCATGG C^CATGG NgoMIV 686 GCCGGC G^CCGGC PflFI 394 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 382 GAGTC GAGTC(4/5) PshAI 1284 GACTTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 907 CAGCTG CAG^CTG ScaI 567 AGTACT AGT^ACT SexAI 983 ACCTGGT A^CC[AT]GGT SmaI 25 CCCGGG CCC^GGG SspBI 797 TGTACA T^GTACA StuI 329 AGGCCT AGG^CCT Tth111I 394 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 25 CCCGGG C^CCGGG XmaI 25 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AfaI 568 GTAC GT^AC 798 GTAC AocI 472 CCTGAGG CC^T[ACGT]AGG 817 CCTGAGG Asp700I 249 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 294 GAACACCTTC BanII 81 GGGCTC G[GA]GC[CT]^C 166 GAGCCC BsaI 5 GAGACC GGTCTC(1/5) 1129 GAGACC BsrBI 1015 GAGCGG CCGCTC(-3/-3) 1168 GAGCGG BsrI 210 ACTGG ACTGG(1/-1) 1265 ACTGG BstF5I 138 CATCC GGATG(2/0) 1143 GGATG BstXI 305 CCATCTACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1003 CCAGAAATCTGG BstYI 484 GGATCC [GA]^GATC[CT] 998 AGATCC Bsu36I 472 CCTGAGG CC^T[ACGT]AGG 817 CCTGAGG Eco81I 472 CCTGAGG CC^T[ACGT]AGG 817 CCTGAGG EcoT14I 192 CCATGG C^C[AT][AT]GG 854 CCAAGG FokI 138 CATCC GGATG(9/13) 1143 GGATG HphI 1306 GGTGA GGTGA(8/7) 188 TCACC MaeIII 187 GTCAC ^GT[ACGT]AC 1307 GTGAC MboII 391 GAAGA GAAGA(8/7) 362 TCTTC MflI 484 GGATCC [GA]^GATC[CT] 998 AGATCC MslI 306 CATCTACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 312 CATGGTGGTG NspI 128 ACATGC [GA]CATG^[CT] 1074 GCATGT RsaI 568 GTAC GT^AC 798 GTAC SfaNI 145 GATGC GCATC(5/9) 1220 GATGC SmlI 615 CTTGAG C^T[CT][GA]AG 868 CTCAAG StyI 192 CCATGG C^C[AT][AT]GG 854 CCAAGG TaqI 143 TCGA T^CGA 716 TCGA Tsp45I 187 GTCAC ^GT[GC]AC 1307 GTGAC Tsp509I 539 AATT ^AATT 1053 AATT TthHB8I 143 TCGA T^CGA 716 TCGA XcmI 305 CCATCTACATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 450 CCAGAGTGGCTGTGG XhoII 484 GGATCC [GA]^GATC[CT] 998 AGATCC XmnI 249 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 294 GAACACCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 59 enzymes) # ######################################### AccII AciI AluI AlwI AlwNI AvaII BbvI BcnI BlpI BmyI BpmI Bpu1102I BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BssKI BstAPI BstNI BstUI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII EcoO109I EcoRII FauI Fnu4HI HaeIII HapII HhaI HinP1I HpaII ItaI MboI MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PpuMI PstI Sau3AI Sau96I ScrFI SfcI TseI TspRI