################################## # NO CUT ENZYMES ( 183 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AflII AflIII AgeI AhdI AlwNI AocI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BclI BcnI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsiYI BslI BsmAI BsmBI BsmFI BsoBI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I Cfr13I CpoI DdeI DraI DraII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HindIII HpaI HpaII KasI KpnI KspI MfeI MluI MluNI MroI MscI MslI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI SwaI TfiI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AcsI 412 AAATTT [GA]^AATT[CT] AluI 226 AGCT AG^CT Alw44I 239 GTGCAC G^TGCAC ApaLI 239 GTGCAC G^TGCAC ApoI 412 AAATTT [GA]^AATT[CT] Asp700I 422 GAAAGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 239 GTGCAC G[AT]GC[AT]^C BbvI 112 GCAGC GCAGC(8/12) BciVI 186 GGATAC GTATCC(6/5) BfaI 355 CTAG C^TAG BmyI 239 GTGCAC G[AGT]GC[ACT]^C BsaBI 30 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiHKAI 239 GTGCAC G[AT]GC[AT]^C BsmI 426 GCATTC GAATGC(1/-1) Bsp1286I 239 GTGCAC G[AGT]GC[ACT]^C BspDI 370 ATCGAT AT^CGAT BsrI 294 ACTGG ACTGG(1/-1) BssKI 517 CCTGG ^CC[ACGT]GG BstF5I 156 GGATG GGATG(2/0) BstNI 517 CCTGG CC^[AT]GG BstYI 29 AGATCC [GA]^GATC[CT] Cac8I 68 GCCTGC GC[ACGT]^[ACGT]GC ClaI 370 ATCGAT AT^CGAT DraIII 242 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG EarI 275 GAAGAG CTCTTC(1/4) EcoRII 517 CCTGG ^CC[AT]GG EcoT14I 579 CCAAGG C^C[AT][AT]GG Fnu4HI 112 GCAGC GC^[ACGT]GC FokI 156 GGATG GGATG(9/13) HincII 127 GTTGAC GT[CT]^[GA]AC HindII 127 GTTGAC GT[CT]^[GA]AC HinfI 551 GAGTC G^A[ACGT]TC HphI 138 GGTGA GGTGA(8/7) ItaI 112 GCAGC GC^[ACGT]GC Ksp632I 275 GAAGAG CTCTTC(1/4) MaeI 355 CTAG C^TAG MamI 30 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 29 AGATCC [GA]^GATC[CT] MvaI 517 CCTGG CC^[AT]GG NlaIV 589 GGAGCC GG[ACGT]^[ACGT]CC NspI 350 ACATGC [GA]CATG^[CT] PleI 551 GAGTC GAGTC(4/5) ScrFI 517 CCTGG CC^[ACGT]GG SmlI 75 CTTGAG C^T[CT][GA]AG StuI 405 AGGCCT AGG^CCT StyI 579 CCAAGG C^C[AT][AT]GG TseI 112 GCAGC G^C[AT]GC TspRI 152 CAGTG CA[GC]TG[ACGT][ACGT]^ XhoII 29 AGATCC [GA]^GATC[CT] XmnI 422 GAAAGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AfaI 329 GTAC GT^AC 472 GTAC AlwI 30 GATCC GGATC(4/5) 100 GATCC BsaJI 516 CCCTGG C^C[ACGT][ACGT]GG 579 CCAAGG BseRI 584 GAGGAG GAGGAG(10/8) 587 GAGGAG Bsp1407I 328 TGTACA T^GTACA 471 TGTACA BsrGI 328 TGTACA T^GTACA 471 TGTACA HaeIII 406 GGCC GG^CC 577 GGCC MaeII 11 ACGT A^CGT 94 ACGT MaeIII 268 GTCAC ^GT[ACGT]AC 561 GTCAC MwoI 59 GCAAGTCCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 169 GCTAAACTTGC NlaIII 351 CATG CATG^ 432 CATG RsaI 329 GTAC GT^AC 472 GTAC SspBI 328 TGTACA T^GTACA 471 TGTACA TaqI 371 TCGA T^CGA 392 TCGA Tsp45I 268 GTCAC ^GT[GC]AC 561 GTCAC TthHB8I 371 TCGA T^CGA 392 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 9 enzymes) # ######################################### DpnII MboI MboII MnlI MseI NdeII Sau3AI Tru9I Tsp509I