################################## # NO CUT ENZYMES ( 152 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BanI BbeI BbrPI BbvCI BcgI BciVI BclI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I CelII CfoI ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I KasI KpnI Ksp632I KspI MaeII MfeI MluI MroI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tsp509I Tth111I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccII 121 CGCG CG^CG AfaI 379 GTAC GT^AC AflII 481 CTTAAG C^TTAAG AocI 370 CCTGAGG CC^T[ACGT]AGG AspHI 759 GAGCTC G[AT]GC[AT]^C BalI 24 TGGCCA TGG^CCA BcnI 212 CCCGG CC^[GC]GG BfaI 473 CTAG C^TAG BfrI 481 CTTAAG C^TTAAG BsaBI 877 GATAGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiHKAI 759 GAGCTC G[AT]GC[AT]^C BsmAI 259 GAGAC GTCTC(1/5) BsmFI 163 GTCCC GGGAC(10/14) BspHI 405 TCATGA T^CATGA BsrFI 106 ACCGGC [GA]^CCGG[CT] BstAPI 144 GCAGCTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 121 CGCG CG^CG BstXI 566 CCAACGATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 370 CCTGAGG CC^T[ACGT]AGG Cfr10I 106 ACCGGC [GA]^CCGG[CT] EaeI 24 TGGCCA [CT]^GGCC[GA] Eco81I 370 CCTGAGG CC^T[ACGT]AGG HincII 102 GTTAAC GT[CT]^[GA]AC HindII 102 GTTAAC GT[CT]^[GA]AC HpaI 102 GTTAAC GTT^AAC HphI 494 TCACC GGTGA(8/7) MaeI 473 CTAG C^TAG MaeIII 413 GTGAC ^GT[ACGT]AC MamI 877 GATAGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 24 TGGCCA TGG^CCA MscI 24 TGGCCA TGG^CCA MslI 406 CATGAAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 121 CGCG CG^CG NciI 212 CCCGG CC^[GC]GG PstI 349 CTGCAG CTGCA^G RcaI 405 TCATGA T^CATGA RsaI 379 GTAC GT^AC SacI 759 GAGCTC GAGCT^C SmlI 481 CTTAAG C^T[CT][GA]AG StuI 692 AGGCCT AGG^CCT TaqI 390 TCGA T^CGA Tsp45I 413 GTGAC ^GT[GC]AC TthHB8I 390 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AvaII 531 GGACC G^G[AT]CC 554 GGACC BamHI 215 GGATCC G^GATCC 618 GGATCC BanII 358 GAGCCC G[GA]GC[CT]^C 759 GAGCTC BbsI 288 GAAGAC GAAGAC(2/6) 504 GTCTTC BmyI 358 GAGCCC G[AGT]GC[ACT]^C 759 GAGCTC BpuAI 288 GAAGAC GAAGAC(2/6) 504 GTCTTC Bsp1286I 358 GAGCCC G[AGT]GC[ACT]^C 759 GAGCTC BspMI 419 ACCTGC ACCTGC(4/8) 522 ACCTGC BsrDI 110 GCAATG GCAATG(2/0) 153 GCAATG EcoT14I 399 CCTTGG C^C[AT][AT]GG 831 CCTTGG FauI 354 GCGGG CCCGC(4/6) 657 GCGGG HapII 107 CCGG C^CGG 213 CCGG HindIII 91 AAGCTT A^AGCTT 484 AAGCTT HinfI 318 GACTC G^A[ACGT]TC 601 GACTC HpaII 107 CCGG C^CGG 213 CCGG MspI 107 CCGG C^CGG 213 CCGG NlaIII 406 CATG CATG^ 588 CATG PflMI 27 CCAGTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 545 CCAAGCTGTGG PleI 318 GACTC GAGTC(4/5) 601 GACTC SfcI 266 CTGTAG C^T[GA][CT]AG 349 CTGCAG StyI 399 CCTTGG C^C[AT][AT]GG 831 CCTTGG Van91I 27 CCAGTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 545 CCAAGCTGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 41 enzymes) # ######################################### AciI AluI AlwI BbvI BsaJI BsgI BsiYI BslI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeIII ItaI MboI MboII MflI MnlI MseI MspA1I MvaI MwoI NdeII NlaIV PvuII Sau3AI Sau96I ScrFI SfaNI Tru9I TseI TspRI XhoII