################################## # NO CUT ENZYMES ( 179 enzymes) # ################################## AatII AccI AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanII BbeI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT22I FauI FbaI FseI FspI HaeII HhaI Hin1I HinP1I HindIII HpaI KasI KspI MaeI MamI MfeI MflI MluI MluNI MroI MscI MspA1I MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI TfiI Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 31 enzymes) # ################################### AciI 12 GCGG CCGC(-3/-1) BbrPI 681 CACGTG CAC^GTG BbvCI 487 GCTGAGG CCTCAGC(-5/-2) BbvI 586 GCTGC GCAGC(8/12) BcnI 16 CCCGG CC^[GC]GG BsaAI 681 CACGTG [CT]AC^GT[GA] BsiYI 456 CCCACTTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 456 CCCACTTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BspHI 110 TCATGA T^CATGA Cac8I 8 GCCTGC GC[ACGT]^[ACGT]GC EarI 735 GAAGAG CTCTTC(1/4) EcoT14I 289 CCAAGG C^C[AT][AT]GG HapII 17 CCGG C^CGG HgaI 432 GCGTC GACGC(5/10) HincII 154 GTTGAC GT[CT]^[GA]AC HindII 154 GTTGAC GT[CT]^[GA]AC HpaII 17 CCGG C^CGG HphI 300 TCACC GGTGA(8/7) Ksp632I 735 GAAGAG CTCTTC(1/4) MaeII 682 ACGT A^CGT MslI 21 CACTCAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 17 CCGG C^CGG MwoI 718 GCAAATGTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NciI 16 CCCGG CC^[GC]GG PmaCI 681 CACGTG CAC^GTG PmlI 681 CACGTG CAC^GTG RcaI 110 TCATGA T^CATGA SmlI 23 CTCAAG C^T[CT][GA]AG StuI 6 AGGCCT AGG^CCT StyI 289 CCAAGG C^C[AT][AT]GG TseI 586 GCTGC G^C[AT]GC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### Acc65I 199 GGTACC G^GTACC 293 GGTACC AflIII 680 ACACGT A^C[GA][CT]GT 682 ACGTGT AlwI 114 GATCC GGATC(4/5) 475 GATCC Asp718I 199 GGTACC G^GTACC 293 GGTACC AvaII 30 GGACC G^G[AT]CC 528 GGACC BanI 199 GGTACC G^G[CT][GA]CC 293 GGTACC BbsI 509 GAAGAC GAAGAC(2/6) 691 GAAGAC BpuAI 509 GAAGAC GAAGAC(2/6) 691 GAAGAC BseRI 284 CTCCTC GAGGAG(10/8) 259 GAGGAG BsrI 540 ACTGG ACTGG(1/-1) 499 CCAGT DpnII 114 GATC ^GATC 475 GATC EcoRI 424 GAATTC G^AATTC 622 GAATTC Fnu4HI 12 GCGGC GC^[ACGT]GC 586 GCTGC HaeIII 7 GGCC GG^CC 14 GGCC ItaI 12 GCGGC GC^[ACGT]GC 586 GCTGC KpnI 199 GGTACC GGTAC^C 293 GGTACC MboI 114 GATC ^GATC 475 GATC NdeII 114 GATC ^GATC 475 GATC NlaIII 111 CATG CATG^ 159 CATG Sau3AI 114 GATC ^GATC 475 GATC Tsp45I 145 GTGAC ^GT[GC]AC 602 GTGAC TspRI 483 CAGTG CA[GC]TG[ACGT][ACGT]^ 539 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 26 enzymes) # ######################################### AcsI AfaI AluI ApoI BsaJI BsmFI BssKI BstF5I BstNI Cfr13I DdeI EcoRII FokI HinfI MaeIII MboII MnlI MseI MvaI NlaIV PleI RsaI Sau96I ScrFI Tru9I Tsp509I