(Circular) (Six-base) MAPSORT of: pFN21AB8317.seq Check: 9613 from: 1 to: 6163 pFN21AB8317 6163 bp With 182 enzymes: SgfI * February 27, 2009 14:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2617 2617 Size: 6163 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3973 278 Size: 53 83 186 936 2437 2468 Fragments arranged by size: 2468 2437 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3506 1937 Size: 1569 4594 Acc65I G'GTAC_C Cuts at: 3484 3484 Size: 6163 AclI AA'CG_TT Cuts at: 1563 4290 4663 1563 Size: 2727 373 3063 Fragments arranged by size: 3063 2727 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2328 3032 4226 5610 5878 1632 Size: 696 704 1194 1384 268 1917 Fragments arranged by size: 1917 1384 1194 704 696 268 AflII C'TTAA_G Cuts at: 820 1017 2327 820 Size: 197 1310 4656 Fragments arranged by size: 4656 1310 197 AflIII A'CryG_T Cuts at: 1203 5062 6039 1203 Size: 3859 977 1327 Fragments arranged by size: 3859 1327 977 AgeI A'CCGG_T Cuts at: 4994 4994 Size: 6163 AhdI GACnn_n'nnGTC Cuts at: 4892 4892 Size: 6163 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6163 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6163 AlwNI CAG_nnn'CTG Cuts at: 2555 2840 3240 3906 5478 2555 Size: 285 400 666 1572 3240 Fragments arranged by size: 3240 1572 666 400 285 ApaLI G'TGCA_C Cuts at: 4220 5376 4220 Size: 1156 5007 ApoI r'AATT_y Cuts at: 1493 3472 3698 3850 5959 1493 Size: 1979 226 152 2109 1697 Fragments arranged by size: 2109 1979 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4717 160 Size: 872 3685 1606 Fragments arranged by size: 3685 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6163 AvaI C'yCGr_G Cuts at: 1870 1943 2928 3182 3488 1870 Size: 73 985 254 306 4545 Fragments arranged by size: 4545 985 306 254 73 BamHI G'GATC_C Cuts at: 3493 3860 3493 Size: 367 5796 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2588 3484 4939 618 Size: 325 217 627 801 896 1455 1842 Fragments arranged by size: 1842 1455 896 801 627 325 217 BanII G_rGCy'C Cuts at: 1391 2028 2139 2172 2498 3029 3183 3191 3219 Size: 637 111 33 326 531 154 8 28 Cuts at: 3219 3482 1391 Size: 263 4072 Fragments arranged by size: 4072 637 531 326 263 154 111 33 28 8 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2052 2868 3020 928 Size: 971 153 816 152 4071 Fragments arranged by size: 4071 971 816 153 152 BbvCI CC'TCA_GC Cuts at: 2706 2775 2706 Size: 69 6094 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4354 4388 1903 Size: 34 2417 34 3678 Fragments arranged by size: 3678 2417 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4055 5265 4055 Size: 1210 4953 BclI T'GATC_A Cuts at: 1548 2376 2745 1548 Size: 828 369 4966 Fragments arranged by size: 4966 828 369 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2849 3338 4774 Size: 107 122 71 631 605 1177 489 1436 Cuts at: 4774 136 Size: 1525 Fragments arranged by size: 1525 1436 1177 631 605 489 122 107 71 BglII A'GATC_T Cuts at: 6158 6158 Size: 6163 BlpI GC'TnA_GC Cuts at: 2166 3580 2166 Size: 1414 4749 Bme1580I G_kGCm'C Cuts at: 1790 4224 5380 1790 Size: 2434 1156 2573 Fragments arranged by size: 2573 2434 1156 BmgBI CAC'GTC Cuts at: 1318 2613 1318 Size: 1295 4868 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2493 3894 4852 Size: 416 53 83 399 24 1076 1401 958 Cuts at: 4852 442 Size: 1753 Fragments arranged by size: 1753 1401 1076 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6163 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2042 2532 2663 3296 4823 1239 Size: 803 490 131 633 1527 2579 Fragments arranged by size: 2579 1527 803 633 490 131 Bpu10I CC'TnA_GC Cuts at: 2027 2706 2775 3177 3213 2027 Size: 679 69 402 36 4977 Fragments arranged by size: 4977 679 402 69 36 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2051 2066 3247 4288 5153 5451 2051 Size: 15 1181 1041 865 298 2763 Fragments arranged by size: 2763 1181 1041 865 298 15 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2590 2891 4826 882 Size: 617 1091 301 1935 2219 Fragments arranged by size: 2219 1935 1091 617 301 BsaAI yAC'GTr Cuts at: 493 1204 3954 493 Size: 711 2750 2702 Fragments arranged by size: 2750 2702 711 BsaBI GATnn'nnATC Cuts at: 6157 6157 Size: 6163 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2572 3440 3970 4352 Size: 53 83 186 936 1039 868 530 382 Cuts at: 4352 4987 275 Size: 635 1451 Fragments arranged by size: 1451 1039 936 868 635 530 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4596 4994 5268 5415 5826 4596 Size: 398 274 147 411 4933 Fragments arranged by size: 4933 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5938 Fragments arranged by size: 5938 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3070 3263 3279 3434 1186 Size: 1884 193 16 155 3915 Fragments arranged by size: 3915 1884 193 155 16 BseYI C'CCAG_C Cuts at: 2012 2354 5366 5862 2012 Size: 342 3012 496 2313 Fragments arranged by size: 3012 2313 496 342 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2162 2645 2912 1163 Size: 573 426 483 267 4414 Fragments arranged by size: 4414 573 483 426 267 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3342 3613 4374 5402 1540 Size: 275 186 1341 271 761 1028 2301 Fragments arranged by size: 2301 1341 1028 761 275 271 186 BsiHKAI G_wGCw'C Cuts at: 3102 3191 4224 4309 5380 3102 Size: 89 1033 85 1071 3885 Fragments arranged by size: 3885 1071 1033 89 85 BsmI GAATG_Cn' Cuts at: 3674 3767 3674 Size: 93 6070 BsmBI CGTCTCn'nnnn_ Cuts at: 2927 2927 Size: 6163 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2028 2139 2172 2498 3029 3102 3183 Size: 399 238 111 33 326 531 73 81 Cuts at: 3183 3191 3219 3482 4224 4309 5380 1391 Size: 8 28 263 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 531 399 326 263 238 111 85 81 73 33 28 8 BspEI T'CCGG_A Cuts at: 5826 5826 Size: 6163 BspHI T'CATG_A Cuts at: 4052 5782 4052 Size: 1730 4433 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2617 3518 844 Size: 1773 901 3489 Fragments arranged by size: 3489 1773 901 BsrBI CCG'CTC Cuts at: 1394 1823 2349 2586 3000 4050 1394 Size: 429 526 237 414 1050 3507 Fragments arranged by size: 3507 1050 526 429 414 237 BsrDI GCAATG_nn' Cuts at: 65 1248 4658 4832 65 Size: 1183 3410 174 1396 Fragments arranged by size: 3410 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4807 4994 1700 Size: 110 2997 187 2869 Fragments arranged by size: 2997 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6163 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6163 BssSI C'ACGA_G Cuts at: 2593 4223 5235 2593 Size: 1630 1012 3521 Fragments arranged by size: 3521 1630 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2840 3240 794 Size: 2046 400 3717 Fragments arranged by size: 3717 2046 400 BstBI TT'CG_AA Cuts at: 2430 4967 2430 Size: 2537 3626 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6163 BstYI r'GATC_y Cuts at: 1972 3493 3860 4245 4262 5714 5800 5812 6158 Size: 1521 367 385 17 1452 86 12 346 Cuts at: 6158 1972 Size: 1977 Fragments arranged by size: 1977 1521 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2787 2787 Size: 6163 BtgI C'CryG_G Cuts at: 513 1065 2004 3318 3336 513 Size: 552 939 1314 18 3340 Fragments arranged by size: 3340 1314 939 552 18 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5424 Fragments arranged by size: 5424 683 56 BtsI GCAGTG_nn' Cuts at: 3146 3679 4473 4493 3146 Size: 533 794 20 4816 Fragments arranged by size: 4816 794 533 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6163 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6128 DraI TTT'AAA Cuts at: 2089 3467 2089 Size: 1378 4785 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5170 809 Size: 507 3854 1802 Fragments arranged by size: 3854 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2344 2607 3339 Size: 54 1425 50 275 18 514 263 732 Cuts at: 3339 3610 4499 6128 8 Size: 271 889 1629 43 Fragments arranged by size: 1629 1425 889 732 514 275 271 263 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3339 3610 1537 Size: 275 1527 271 4090 Fragments arranged by size: 4090 1527 275 271 EarI CTCTTCn'nnn_ Cuts at: 1353 2109 2990 4093 1353 Size: 756 881 1103 3423 Fragments arranged by size: 3423 1103 881 756 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2203 3049 4750 5124 5270 387 Size: 1064 138 614 846 1701 374 146 1280 Fragments arranged by size: 1701 1280 1064 846 614 374 146 138 EcoICRI GAG'CTC Cuts at: 3189 3189 Size: 6163 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2042 2328 2532 2663 3032 3296 4226 Size: 393 410 286 204 131 369 264 930 Cuts at: 4226 4823 5610 5878 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 930 787 597 410 393 369 286 268 264 204 131 EcoO109I rG'GnC_Cy Cuts at: 2053 2062 2279 2638 3136 3196 2053 Size: 9 217 359 498 60 5020 Fragments arranged by size: 5020 498 359 217 60 9 EcoRI G'AATT_C Cuts at: 3472 3850 3472 Size: 378 5785 FspI TGC'GCA Cuts at: 4669 4669 Size: 6163 HaeII r_GCGC'y Cuts at: 1678 2260 3078 5310 1678 Size: 582 818 2232 2531 Fragments arranged by size: 2531 2232 818 582 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4804 4836 4878 4910 1373 Size: 32 135 32 3232 32 42 32 2626 Fragments arranged by size: 3232 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3507 3753 669 Size: 988 281 1569 246 3079 Fragments arranged by size: 3079 1569 988 281 246 HindIII A'AGCT_T Cuts at: 748 2232 2324 748 Size: 1484 92 4587 Fragments arranged by size: 4587 1484 92 HpaI GTT'AAC Cuts at: 3753 3753 Size: 6163 KpnI G_GTAC'C Cuts at: 3488 3488 Size: 6163 MfeI C'AATT_G Cuts at: 3762 3762 Size: 6163 MluI A'CGCG_T Cuts at: 6039 6039 Size: 6163 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5277 5461 1305 Size: 3972 184 2007 Fragments arranged by size: 3972 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4341 Fragments arranged by size: 4341 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4121 4480 4639 518 Size: 648 2955 359 159 2042 Fragments arranged by size: 2955 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2254 2840 3320 3579 4256 5404 5649 1606 Size: 648 586 480 259 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 648 586 480 259 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4672 Fragments arranged by size: 4672 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6163 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6163 NotI GC'GGCC_GC Cuts at: 3610 3610 Size: 6163 NspI r_CATG'y Cuts at: 1132 1229 3521 5066 1132 Size: 97 2292 1545 2229 Fragments arranged by size: 2292 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2386 3029 1348 Size: 1038 643 4482 Fragments arranged by size: 4482 1038 643 PciI A'CATG_T Cuts at: 5062 5062 Size: 6163 PflMI CCAn_nnn'nTGG Cuts at: 1108 2555 2740 1108 Size: 1447 185 4531 Fragments arranged by size: 4531 1447 185 PfoI T'CCnGG_A Cuts at: 2548 2640 2548 Size: 92 6071 PmeI GTTT'AAAC Cuts at: 3467 3467 Size: 6163 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4232 4264 5745 5777 4232 Size: 32 1481 32 4618 Fragments arranged by size: 4618 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2053 2279 2638 3196 2053 Size: 226 359 558 5020 Fragments arranged by size: 5020 558 359 226 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6163 PsiI TTA'TAA Cuts at: 3733 3733 Size: 6163 PstI C_TGCA'G Cuts at: 830 3515 830 Size: 2685 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6163 SacI G_AGCT'C Cuts at: 3191 3191 Size: 6163 SacII CC_GC'GG Cuts at: 3321 3321 Size: 6163 SalI G'TCGA_C Cuts at: 1936 3505 1936 Size: 1569 4594 SanDI GG'GwC_CC Cuts at: 2279 2279 Size: 6163 SapI GCTCTTCn'nnn_ Cuts at: 2990 2990 Size: 6163 SbfI CC_TGCA'GG Cuts at: 3515 3515 Size: 6163 ScaI AGT'ACT Cuts at: 4411 6123 4411 Size: 1712 4451 SfcI C'TryA_G Cuts at: 826 1045 3511 4646 5327 5518 826 Size: 219 2466 1135 681 191 1471 Fragments arranged by size: 2466 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6163 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6163 SmaI CCC'GGG Cuts at: 1872 2930 3490 1872 Size: 1058 560 4545 Fragments arranged by size: 4545 1058 560 SmlI C'TyrA_G Cuts at: 820 1017 1943 2066 2081 2327 3226 4267 5168 Size: 197 926 123 15 246 899 1041 901 Cuts at: 5168 5430 820 Size: 262 1553 Fragments arranged by size: 1553 1041 926 901 899 262 246 197 123 15 SnaBI TAC'GTA Cuts at: 493 3954 493 Size: 3461 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6163 SphI G_CATG'C Cuts at: 1132 3521 1132 Size: 2389 3774 SspI AAT'ATT Cuts at: 5 52 4087 5 Size: 47 4035 2081 Fragments arranged by size: 4035 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6163 StyI C'CwwG_G Cuts at: 513 1065 2004 2601 3057 3294 3404 3602 513 Size: 552 939 597 456 237 110 198 3074 Fragments arranged by size: 3074 939 597 552 456 237 198 110 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2747 3464 4190 4207 4360 4545 4990 Size: 1255 1167 717 726 17 153 185 445 Cuts at: 4990 325 Size: 1498 Fragments arranged by size: 1498 1255 1167 726 717 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3351 4409 6121 Size: 274 80 33 51 1443 1374 1058 1712 Cuts at: 6121 96 Size: 138 Fragments arranged by size: 1712 1443 1374 1058 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3110 3142 6123 6155 3110 Size: 32 2981 32 3118 Fragments arranged by size: 3118 2981 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6163 XbaI T'CTAG_A Cuts at: 3499 3499 Size: 6163 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2737 2737 Size: 6163 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6163 XmaI C'CCGG_G Cuts at: 1870 2928 3488 1870 Size: 1058 560 4545 Fragments arranged by size: 4545 1058 560 XmnI GAAnn'nnTTC Cuts at: 4292 4292 Size: 6163 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3971 276 Size: 53 83 186 936 2437 2468 Fragments arranged by size: 2468 2437 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SacII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SexAI SfiI SfoI SrfI SwaI