Restriction map for
pFN21AB8315
##################################
# NO CUT ENZYMES ( 151 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BalI
BamHI BanI BbeI BbrPI BbvCI
BclI BfrI BglI BlnI BlpI
Bpu1102I BsaAI BsaHI BsaWI BseAI
BsgI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DdeI DraI DrdI DsaI
Eam1105I Eco47III Eco81I EcoO65I EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HaeII Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MaeII
MfeI MluI MluNI MroI MscI
MseI MslI MunI MvnI NarI
NcoI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TfiI Tru9I
Tth111I Van91I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AcsI 192 GAATTC [GA]^AATT[CT]
ApoI 192 GAATTC [GA]^AATT[CT]
Asp700I 108 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 322 CCCGAG C^[CT]CG[GA]G
BbsI 420 GTCTTC GAAGAC(2/6)
BcgI 13 GCAGATCTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 24 GGATAC GTATCC(6/5)
BcnI 468 CCGGG CC^[GC]GG
BfaI 200 CTAG C^TAG
BpmI 516 CTCCAG CTGGAG(16/14)
BpuAI 420 GTCTTC GAAGAC(2/6)
BsaBI 406 GATGACGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 124 GAGACC GGTCTC(1/5)
BsiEI 465 CGGCCG CG[GA][CT]^CG
BsmAI 124 GAGAC GTCTC(1/5)
BsoBI 322 CCCGAG C^[CT]CG[GA]G
BsrBI 670 CCGCTC CCGCTC(-3/-3)
BsrI 354 CCAGT ACTGG(1/-1)
CfoI 48 GCGC GCG^C
DraII 665 AGGTCCC [GA]G^G[ACGT]CC[CT]
DraIII 394 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 465 CGGCCG [CT]^GGCC[GA]
EagI 465 CGGCCG C^GGCCG
EclXI 465 CGGCCG C^GGCCG
Eco52I 465 CGGCCG C^GGCCG
EcoNI 73 CCTGTAGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 665 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRI 192 GAATTC G^AATTC
HhaI 48 GCGC GCG^C
HinP1I 48 GCGC G^CGC
HinfI 325 GAGTC G^A[ACGT]TC
MaeI 200 CTAG C^TAG
MaeIII 361 GTGAC ^GT[ACGT]AC
MamI 406 GATGACGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 682 CAGCTG C[AC]G^C[GT]G
NaeI 331 GCCGGC GCC^GGC
NciI 468 CCGGG CC^[GC]GG
NgoMIV 331 GCCGGC G^CCGGC
PleI 325 GAGTC GAGTC(4/5)
PpuMI 665 AGGTCCC [GA]G^G[AT]CC[CT]
PvuII 682 CAGCTG CAG^CTG
SacI 522 GAGCTC GAGCT^C
SexAI 395 ACCAGGT A^CC[AT]GGT
SfcI 74 CTGTAG C^T[GA][CT]AG
Tsp45I 361 GTGAC ^GT[GC]AC
Tsp509I 193 AATT ^AATT
XcmI 518 CCAGGAGCTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 108 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AlwI 82 GGATC GGATC(4/5)
657 GGATC
AlwNI 374 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
682 CAGCTGCTG
AspHI 522 GAGCTC G[AT]GC[AT]^C
632 GTGCTC
AvaII 531 GGACC G^G[AT]CC
666 GGTCC
BanII 319 GAGCCC G[GA]GC[CT]^C
522 GAGCTC
BglII 15 AGATCT A^GATCT
110 AGATCT
BsiHKAI 522 GAGCTC G[AT]GC[AT]^C
632 GTGCTC
BsmFI 470 GGGAC GGGAC(10/14)
667 GTCCC
BsrFI 50 GCCGGT [GA]^CCGG[CT]
331 GCCGGC
BstYI 15 AGATCT [GA]^GATC[CT]
110 AGATCT
Cfr10I 50 GCCGGT [GA]^CCGG[CT]
331 GCCGGC
HgaI 241 GACGC GACGC(5/10)
382 GACGC
MflI 15 AGATCT [GA]^GATC[CT]
110 AGATCT
TaqI 116 TCGA T^CGA
196 TCGA
TspRI 359 CAGTG CA[GC]TG[ACGT][ACGT]^
474 CAGTG
TthHB8I 116 TCGA T^CGA
196 TCGA
XhoII 15 AGATCT [GA]^GATC[CT]
110 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AciI AfaI AluI BbvI BmyI
BsaJI BseRI BsiYI BslI Bsp1286I
BssKI BstF5I BstNI Cac8I Cfr13I
DpnII EarI EcoRII FauI Fnu4HI
FokI HaeIII HapII HpaII HphI
ItaI Ksp632I MboI MboII MnlI
MspI MvaI MwoI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfaNI TseI