(Circular) (Six-base) MAPSORT of: pFN21AB8314.seq Check: 7021 from: 1 to: 5809 pFN21AB8314 5809 bp With 182 enzymes: SgfI * February 27, 2009 14:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2223 2223 Size: 5809 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3619 278 Size: 53 83 186 936 2083 2468 Fragments arranged by size: 2468 2083 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3152 1937 Size: 1215 4594 Acc65I G'GTAC_C Cuts at: 3130 3130 Size: 5809 AclI AA'CG_TT Cuts at: 1563 3936 4309 1563 Size: 2373 373 3063 Fragments arranged by size: 3063 2373 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2267 2389 2648 3872 5256 5524 1632 Size: 635 122 259 1224 1384 268 1917 Fragments arranged by size: 1917 1384 1224 635 268 259 122 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5612 AflIII A'CryG_T Cuts at: 1203 4708 5685 1203 Size: 3505 977 1327 Fragments arranged by size: 3505 1327 977 AgeI A'CCGG_T Cuts at: 4640 4640 Size: 5809 AhdI GACnn_n'nnGTC Cuts at: 4538 4538 Size: 5809 AleI CACnn'nnGTG Cuts at: 1166 2945 1166 Size: 1779 4030 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5809 AlwNI CAG_nnn'CTG Cuts at: 2053 3552 5124 2053 Size: 1499 1572 2738 Fragments arranged by size: 2738 1572 1499 ApaLI G'TGCA_C Cuts at: 3866 5022 3866 Size: 1156 4653 ApoI r'AATT_y Cuts at: 1493 3118 3344 3496 5605 1493 Size: 1625 226 152 2109 1697 Fragments arranged by size: 2109 1697 1625 226 152 AseI AT'TA_AT Cuts at: 160 1032 4363 160 Size: 872 3331 1606 Fragments arranged by size: 3331 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5809 AvaI C'yCGr_G Cuts at: 1870 1943 3134 1870 Size: 73 1191 4545 Fragments arranged by size: 4545 1191 73 AvrII C'CTAG_G Cuts at: 2782 2782 Size: 5809 BamHI G'GATC_C Cuts at: 3139 3506 3139 Size: 367 5442 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2019 2524 2878 3130 4585 Size: 325 217 627 232 505 354 252 1455 Cuts at: 4585 618 Size: 1842 Fragments arranged by size: 1842 1455 627 505 354 325 252 232 217 BanII G_rGCy'C Cuts at: 1391 2319 2496 2550 2850 2892 3128 1391 Size: 928 177 54 300 42 236 4072 Fragments arranged by size: 4072 928 300 236 177 54 42 BbeI G_GCGC'C Cuts at: 2023 2023 Size: 5809 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4838 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4000 4034 1903 Size: 34 2063 34 3678 Fragments arranged by size: 3678 2063 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3701 4911 3701 Size: 1210 4599 BclI T'GATC_A Cuts at: 1548 1548 Size: 5809 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2017 2887 4420 Size: 107 122 71 631 605 345 870 1533 Cuts at: 4420 136 Size: 1525 Fragments arranged by size: 1533 1525 870 631 605 345 122 107 71 BglII A'GATC_T Cuts at: 2265 2379 5804 2265 Size: 114 3425 2270 Fragments arranged by size: 3425 2270 114 BlpI GC'TnA_GC Cuts at: 3226 3226 Size: 5809 Bme1580I G_kGCm'C Cuts at: 1790 2259 2529 2883 3033 3870 5026 1790 Size: 469 270 354 150 837 1156 2573 Fragments arranged by size: 2573 1156 837 469 354 270 150 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5809 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3046 3540 4498 Size: 416 53 83 399 24 1629 494 958 Cuts at: 4498 442 Size: 1753 Fragments arranged by size: 1753 1629 958 494 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5809 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2327 2609 2631 2762 2894 4469 1239 Size: 1088 282 22 131 132 1575 2579 Fragments arranged by size: 2579 1575 1088 282 132 131 22 Bpu10I CC'TnA_GC Cuts at: 2042 2822 2042 Size: 780 5029 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2885 3934 4799 5097 2885 Size: 1049 865 298 3597 Fragments arranged by size: 3597 1049 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2345 2684 4472 882 Size: 617 846 339 1788 2219 Fragments arranged by size: 2219 1788 846 617 339 BsaAI yAC'GTr Cuts at: 493 1204 3600 493 Size: 711 2396 2702 Fragments arranged by size: 2702 2396 711 BsaBI GATnn'nnATC Cuts at: 5803 5803 Size: 5809 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2020 2148 3616 3998 Size: 53 83 186 936 487 128 1468 382 Cuts at: 3998 4633 275 Size: 635 1451 Fragments arranged by size: 1468 1451 936 635 487 382 186 128 83 53 BsaWI w'CCGG_w Cuts at: 2550 4242 4640 4914 5061 5472 2550 Size: 1692 398 274 147 411 2887 Fragments arranged by size: 2887 1692 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2294 2324 2366 2396 1178 Size: 30 165 30 891 30 42 30 4591 Fragments arranged by size: 4591 891 165 42 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2897 1186 Size: 1711 4098 BseYI C'CCAG_C Cuts at: 2477 2776 5012 5508 2477 Size: 299 2236 496 2778 Fragments arranged by size: 2778 2236 496 299 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2909 3065 1163 Size: 573 1173 156 3907 Fragments arranged by size: 3907 1173 573 156 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3259 4020 5048 1540 Size: 275 186 1258 761 1028 2301 Fragments arranged by size: 2301 1258 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2234 2319 2496 2850 3870 3955 5026 2234 Size: 85 177 354 1020 85 1071 3017 Fragments arranged by size: 3017 1071 1020 354 177 85 85 BsmI GAATG_Cn' Cuts at: 3320 3413 3320 Size: 93 5716 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2234 2259 2319 2496 2529 2550 2850 Size: 399 444 25 60 177 33 21 300 Cuts at: 2850 2883 2892 3033 3128 3870 3955 5026 1391 Size: 33 9 141 95 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 444 399 300 177 141 95 85 60 33 33 25 21 9 BspEI T'CCGG_A Cuts at: 2550 5472 2550 Size: 2922 2887 BspHI T'CATG_A Cuts at: 2106 3698 5428 2106 Size: 1592 1730 2487 Fragments arranged by size: 2487 1730 1592 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2223 2301 2649 2757 3164 844 Size: 1379 78 348 108 407 3489 Fragments arranged by size: 3489 1379 407 348 108 78 BsrBI CCG'CTC Cuts at: 1394 1823 3696 1394 Size: 429 1873 3507 Fragments arranged by size: 3507 1873 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4304 4478 65 Size: 1183 3056 174 1396 Fragments arranged by size: 3056 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3049 4453 4640 1700 Size: 110 1239 1404 187 2869 Fragments arranged by size: 2869 1404 1239 187 110 BsrGI T'GTAC_A Cuts at: 96 2769 96 Size: 2673 3136 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5809 BssSI C'ACGA_G Cuts at: 2024 3869 4881 2024 Size: 1845 1012 2952 Fragments arranged by size: 2952 1845 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5809 BstBI TT'CG_AA Cuts at: 4613 4613 Size: 5809 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5809 BstXI CCAn_nnnn'nTGG Cuts at: 2287 2485 2740 2287 Size: 198 255 5356 Fragments arranged by size: 5356 255 198 BstYI r'GATC_y Cuts at: 1972 2265 2379 2388 2460 3139 3506 3891 3908 Size: 293 114 9 72 679 367 385 17 Cuts at: 3908 5360 5446 5458 5804 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 679 385 367 346 293 114 86 72 17 12 9 Bsu36I CC'TnA_GG Cuts at: 2143 2884 2143 Size: 741 5068 BtgI C'CryG_G Cuts at: 513 1065 2970 513 Size: 552 1905 3352 Fragments arranged by size: 3352 1905 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5070 Fragments arranged by size: 5070 683 56 BtsI GCAGTG_nn' Cuts at: 2206 2411 3325 4119 4139 2206 Size: 205 914 794 20 3876 Fragments arranged by size: 3876 914 794 205 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5809 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5774 DraI TTT'AAA Cuts at: 3113 3113 Size: 5809 DraIII CAC_nnn'GTG Cuts at: 2455 2455 Size: 5809 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4816 809 Size: 507 3500 1802 Fragments arranged by size: 3500 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2871 3051 3081 Size: 54 1425 50 275 18 1041 180 30 Cuts at: 3081 3256 4145 5774 8 Size: 175 889 1629 43 Fragments arranged by size: 1629 1425 1041 889 275 180 175 54 50 43 30 18 EagI C'GGCC_G Cuts at: 1537 1812 3256 1537 Size: 275 1444 4090 Fragments arranged by size: 4090 1444 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3020 3739 1353 Size: 1667 719 3423 Fragments arranged by size: 3423 1667 719 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4396 4770 4916 387 Size: 1064 138 2807 374 146 1280 Fragments arranged by size: 2807 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2317 2494 2848 2317 Size: 177 354 5278 Fragments arranged by size: 5278 354 177 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2267 2327 2389 2609 2631 2648 2762 Size: 393 635 60 62 220 22 17 114 Cuts at: 2762 2894 3872 4469 5256 5524 1239 Size: 132 978 597 787 268 1524 Fragments arranged by size: 1524 978 787 635 597 393 268 220 132 114 62 60 22 17 EcoNI CCTnn'n_nnAGG Cuts at: 2045 2045 Size: 5809 EcoO109I rG'GnC_Cy Cuts at: 2050 2746 2050 Size: 696 5113 EcoRI G'AATT_C Cuts at: 3118 3496 3118 Size: 378 5431 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2347 2347 Size: 5809 FspI TGC'GCA Cuts at: 2645 4315 2645 Size: 1670 4139 HaeII r_GCGC'y Cuts at: 1678 2023 2136 2187 4956 1678 Size: 345 113 51 2769 2531 Fragments arranged by size: 2769 2531 345 113 51 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2147 2179 4450 4482 4524 Size: 32 135 32 575 32 2271 32 42 Cuts at: 4524 4556 1373 Size: 32 2626 Fragments arranged by size: 2626 2271 575 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2956 3153 3399 669 Size: 988 281 1018 197 246 3079 Fragments arranged by size: 3079 1018 988 281 246 197 HindIII A'AGCT_T Cuts at: 748 2124 748 Size: 1376 4433 HpaI GTT'AAC Cuts at: 3399 3399 Size: 5809 KasI G'GCGC_C Cuts at: 2019 2019 Size: 5809 KpnI G_GTAC'C Cuts at: 3134 3134 Size: 5809 MfeI C'AATT_G Cuts at: 3408 3408 Size: 5809 MluI A'CGCG_T Cuts at: 5685 5685 Size: 5809 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2259 2955 4923 5107 1305 Size: 954 696 1968 184 2007 Fragments arranged by size: 2007 1968 954 696 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2873 3083 10 Size: 54 1425 343 1041 210 2736 Fragments arranged by size: 2736 1425 1041 343 210 54 MslI CAynn'nnrTG Cuts at: 518 1166 2570 2945 3767 4126 4285 518 Size: 648 1404 375 822 359 159 2042 Fragments arranged by size: 2042 1404 822 648 375 359 159 MspA1I CmG'CkG Cuts at: 1606 2544 3063 3225 3902 5050 5295 1606 Size: 938 519 162 677 1148 245 2120 Fragments arranged by size: 2120 1148 938 677 519 245 162 NaeI GCC'GGC Cuts at: 3051 3051 Size: 5809 NarI GG'CG_CC Cuts at: 2020 2020 Size: 5809 NcoI C'CATG_G Cuts at: 513 1065 2970 513 Size: 552 1905 3352 Fragments arranged by size: 3352 1905 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5809 NgoMIV G'CCGG_C Cuts at: 3049 3049 Size: 5809 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5809 NotI GC'GGCC_GC Cuts at: 3256 3256 Size: 5809 NspI r_CATG'y Cuts at: 1132 1229 3167 4712 1132 Size: 97 1938 1545 2229 Fragments arranged by size: 2229 1938 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5809 PciI A'CATG_T Cuts at: 4708 4708 Size: 5809 PflMI CCAn_nnn'nTGG Cuts at: 1108 2455 1108 Size: 1347 4462 PfoI T'CCnGG_A Cuts at: 2151 2841 2151 Size: 690 5119 PmeI GTTT'AAAC Cuts at: 3113 3113 Size: 5809 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3878 3910 5391 5423 3878 Size: 32 1481 32 4264 Fragments arranged by size: 4264 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2746 2746 Size: 5809 PshAI GACnn'nnGTC Cuts at: 1613 2241 2964 1613 Size: 628 723 4458 Fragments arranged by size: 4458 723 628 PsiI TTA'TAA Cuts at: 3379 3379 Size: 5809 PstI C_TGCA'G Cuts at: 830 2298 2427 3161 830 Size: 1468 129 734 3478 Fragments arranged by size: 3478 1468 734 129 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5809 SacI G_AGCT'C Cuts at: 2319 2496 2850 2319 Size: 177 354 5278 Fragments arranged by size: 5278 354 177 SalI G'TCGA_C Cuts at: 1936 3151 1936 Size: 1215 4594 SbfI CC_TGCA'GG Cuts at: 3161 3161 Size: 5809 ScaI AGT'ACT Cuts at: 4057 5769 4057 Size: 1712 4097 SexAI A'CCwGG_T Cuts at: 2451 2451 Size: 5809 SfcI C'TryA_G Cuts at: 826 1045 2179 2294 2423 3157 4292 4973 5164 Size: 219 1134 115 129 734 1135 681 191 Cuts at: 5164 826 Size: 1471 Fragments arranged by size: 1471 1135 1134 734 681 219 191 129 115 SfoI GGC'GCC Cuts at: 2021 2021 Size: 5809 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5809 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5809 SmaI CCC'GGG Cuts at: 1872 3136 1872 Size: 1264 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2900 3913 4814 5076 820 Size: 197 926 957 1013 901 262 1553 Fragments arranged by size: 1553 1013 957 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3600 493 Size: 3107 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5809 SphI G_CATG'C Cuts at: 1132 3167 1132 Size: 2035 3774 SspI AAT'ATT Cuts at: 5 52 3733 5 Size: 47 3681 2081 Fragments arranged by size: 3681 2081 47 StuI AGG'CCT Cuts at: 1517 2402 1517 Size: 885 4924 StyI C'CwwG_G Cuts at: 513 1065 2529 2782 2970 3248 513 Size: 552 1464 253 188 278 3074 Fragments arranged by size: 3074 1464 552 278 253 188 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2188 2939 3836 3853 4006 4191 4636 Size: 1255 608 751 897 17 153 185 445 Cuts at: 4636 325 Size: 1498 Fragments arranged by size: 1498 1255 897 751 608 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2769 4055 5767 Size: 274 80 33 51 1443 792 1286 1712 Cuts at: 5767 96 Size: 138 Fragments arranged by size: 1712 1443 1286 792 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5769 5801 5769 Size: 32 5777 Tth111I GACn'n_nGTC Cuts at: 1316 2165 1316 Size: 849 4960 XbaI T'CTAG_A Cuts at: 3145 3145 Size: 5809 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2449 2485 2449 Size: 36 5773 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5809 XmaI C'CCGG_G Cuts at: 1870 3134 1870 Size: 1264 4545 XmnI GAAnn'nnTTC Cuts at: 2402 3938 2402 Size: 1536 4273 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3617 276 Size: 53 83 186 936 2083 2468 Fragments arranged by size: 2468 2083 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI BaeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I EcoRV FseI FspAI NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SfiI SrfI SwaI