################################## # NO CUT ENZYMES ( 205 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BanI BanII BbeI BbrPI BbsI BbvCI BbvI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaJI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssKI BssSI Bst1107I BstAPI BstBI BstEII BstNI BstPI BstXI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoT14I EcoT22I FauI FbaI Fnu4HI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HindIII HpaI ItaI KasI KpnI Ksp632I KspI MaeI MaeII MaeIII MamI MfeI MluI MluNI MroI MscI MseI MslI MunI MvaI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScrFI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tru9I TseI Tsp45I Tsp509I Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AccII 54 CGCG CG^CG AfaI 144 GTAC GT^AC AvaII 21 GGACC G^G[AT]CC BamHI 200 GGATCC G^GATCC BsaI 105 GGTCTC GGTCTC(1/5) BsaWI 87 TCCGGT [AT]^CCGG[AT] BsmAI 106 GTCTC GTCTC(1/5) BstF5I 8 GGATG GGATG(2/0) BstUI 54 CGCG CG^CG BstYI 200 GGATCC [GA]^GATC[CT] Cfr13I 21 GGACC G^G[ACGT]CC EcoRV 160 GATATC GAT^ATC FokI 8 GGATG GGATG(9/13) HaeIII 321 GGCC GG^CC HapII 88 CCGG C^CGG HincII 124 GTTGAC GT[CT]^[GA]AC HindII 124 GTTGAC GT[CT]^[GA]AC HpaII 88 CCGG C^CGG HphI 90 GGTGA GGTGA(8/7) MflI 200 GGATCC [GA]^GATC[CT] MnlI 19 GAGG CCTC(7/6) MspA1I 327 CAGCGG C[AC]G^C[GT]G MspI 88 CCGG C^CGG MvnI 54 CGCG CG^CG NlaIII 180 CATG CATG^ PflMI 24 CCACTCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 49 GAGTC GAGTC(4/5) RsaI 144 GTAC GT^AC Sau96I 21 GGACC G^G[ACGT]CC ScaI 143 AGTACT AGT^ACT SfaNI 41 GATGC GCATC(5/9) SfcI 324 CTACAG C^T[GA][CT]AG TaqI 63 TCGA T^CGA TspRI 165 CACTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 63 TCGA T^CGA Van91I 24 CCACTCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 260 CCAGCCTAACTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 200 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 4 enzymes) # ################################### AciI 53 CCGC CCGC(-3/-1) 329 GCGG AluI 206 AGCT AG^CT 278 AGCT BsmFI 273 GGGAC GGGAC(10/14) 250 GTCCC TfiI 60 GATTC G^A[AT]TC 308 GATTC ######################################### # 3 OR MORE CUT ENZYMES ( 11 enzymes) # ######################################### AlwI BsiYI BslI DdeI DpnII HinfI MboI MboII NdeII NlaIV Sau3AI