(Circular) (Six-base) MAPSORT of: pFN21AB8311.seq Check: 8080 from: 1 to: 5056 pFN21AB8311 5056 bp With 182 enzymes: SgfI * February 27, 2009 14:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2866 278 Size: 53 83 186 936 1330 2468 Fragments arranged by size: 2468 1330 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2399 1937 Size: 462 4594 Acc65I G'GTAC_C Cuts at: 2377 2377 Size: 5056 AclI AA'CG_TT Cuts at: 1563 3183 3556 1563 Size: 1620 373 3063 Fragments arranged by size: 3063 1620 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3119 4503 4771 1632 Size: 1487 1384 268 1917 Fragments arranged by size: 1917 1487 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4859 AflIII A'CryG_T Cuts at: 1203 3955 4932 1203 Size: 2752 977 1327 Fragments arranged by size: 2752 1327 977 AgeI A'CCGG_T Cuts at: 3887 3887 Size: 5056 AhdI GACnn_n'nnGTC Cuts at: 3785 3785 Size: 5056 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5056 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5056 AlwNI CAG_nnn'CTG Cuts at: 2799 4371 2799 Size: 1572 3484 ApaLI G'TGCA_C Cuts at: 3113 4269 3113 Size: 1156 3900 ApoI r'AATT_y Cuts at: 1493 2365 2591 2743 4852 1493 Size: 872 226 152 2109 1697 Fragments arranged by size: 2109 1697 872 226 152 AseI AT'TA_AT Cuts at: 160 1032 3610 160 Size: 872 2578 1606 Fragments arranged by size: 2578 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5056 AvaI C'yCGr_G Cuts at: 1870 1943 2381 1870 Size: 73 438 4545 Fragments arranged by size: 4545 438 73 BamHI G'GATC_C Cuts at: 2205 2386 2753 2205 Size: 181 367 4508 Fragments arranged by size: 4508 367 181 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2377 3832 618 Size: 325 217 627 590 1455 1842 Fragments arranged by size: 1842 1455 627 590 325 217 BanII G_rGCy'C Cuts at: 1391 2375 1391 Size: 984 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4085 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3247 3281 1903 Size: 34 1310 34 3678 Fragments arranged by size: 3678 1310 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2948 4158 2948 Size: 1210 3846 BclI T'GATC_A Cuts at: 1548 1548 Size: 5056 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3667 136 Size: 107 122 71 631 605 1995 1525 Fragments arranged by size: 1995 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5051 5051 Size: 5056 BlpI GC'TnA_GC Cuts at: 2473 2473 Size: 5056 Bme1580I G_kGCm'C Cuts at: 1790 3117 4273 1790 Size: 1327 1156 2573 Fragments arranged by size: 2573 1327 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5056 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2787 3745 442 Size: 416 53 83 399 24 1370 958 1753 Fragments arranged by size: 1753 1370 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5056 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2047 2047 Size: 5056 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3716 1239 Size: 2477 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3181 4046 4344 3181 Size: 865 298 3893 Fragments arranged by size: 3893 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2116 3719 882 Size: 617 617 1603 2219 Fragments arranged by size: 2219 1603 617 617 BsaAI yAC'GTr Cuts at: 493 1204 2847 493 Size: 711 1643 2702 Fragments arranged by size: 2702 1643 711 BsaBI GATnn'nnATC Cuts at: 5050 5050 Size: 5056 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2863 3245 3880 275 Size: 53 83 186 936 1330 382 635 1451 Fragments arranged by size: 1451 1330 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2092 3489 3887 4161 4308 4719 2092 Size: 1397 398 274 147 411 2429 Fragments arranged by size: 2429 1397 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4831 Fragments arranged by size: 4831 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5056 BseYI C'CCAG_C Cuts at: 4259 4755 4259 Size: 496 4560 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4483 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2506 3267 4295 1540 Size: 275 186 505 761 1028 2301 Fragments arranged by size: 2301 1028 761 505 275 186 BsiHKAI G_wGCw'C Cuts at: 3117 3202 4273 3117 Size: 85 1071 3900 Fragments arranged by size: 3900 1071 85 BsmI GAATG_Cn' Cuts at: 2567 2660 2567 Size: 93 4963 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2375 3117 3202 4273 1391 Size: 399 585 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 585 399 85 BspEI T'CCGG_A Cuts at: 4719 4719 Size: 5056 BspHI T'CATG_A Cuts at: 2945 4675 2945 Size: 1730 3326 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2411 844 Size: 1567 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2943 1394 Size: 429 1120 3507 Fragments arranged by size: 3507 1120 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3551 3725 65 Size: 1183 2303 174 1396 Fragments arranged by size: 2303 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3700 3887 1700 Size: 110 1890 187 2869 Fragments arranged by size: 2869 1890 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5056 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5056 BssSI C'ACGA_G Cuts at: 3116 4128 3116 Size: 1012 4044 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5056 BstBI TT'CG_AA Cuts at: 3860 3860 Size: 5056 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5056 BstYI r'GATC_y Cuts at: 1972 2205 2386 2753 3138 3155 4607 4693 4705 Size: 233 181 367 385 17 1452 86 12 Cuts at: 4705 5051 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 385 367 346 233 181 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4504 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4317 Fragments arranged by size: 4317 683 56 BtsI GCAGTG_nn' Cuts at: 2572 3366 3386 2572 Size: 794 20 4242 Fragments arranged by size: 4242 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5056 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5021 DraI TTT'AAA Cuts at: 2360 2360 Size: 5056 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4063 809 Size: 507 2747 1802 Fragments arranged by size: 2747 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2503 3392 5021 Size: 54 1425 50 275 18 673 889 1629 Cuts at: 5021 8 Size: 43 Fragments arranged by size: 1629 1425 889 673 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2503 1537 Size: 275 691 4090 Fragments arranged by size: 4090 691 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2986 1353 Size: 1633 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3643 4017 4163 387 Size: 1064 138 2054 374 146 1280 Fragments arranged by size: 2054 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3119 3716 4503 4771 1239 Size: 393 1487 597 787 268 1524 Fragments arranged by size: 1524 1487 787 597 393 268 EcoRI G'AATT_C Cuts at: 2365 2743 2365 Size: 378 4678 EcoRV GAT'ATC Cuts at: 2167 2167 Size: 5056 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2248 2248 Size: 5056 FspI TGC'GCA Cuts at: 3562 3562 Size: 5056 HaeII r_GCGC'y Cuts at: 1678 4203 1678 Size: 2525 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3697 3729 3771 3803 1373 Size: 32 135 32 2125 32 42 32 2626 Fragments arranged by size: 2626 2125 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2131 2400 2646 669 Size: 988 281 193 269 246 3079 Fragments arranged by size: 3079 988 281 269 246 193 HindIII A'AGCT_T Cuts at: 748 748 Size: 5056 HpaI GTT'AAC Cuts at: 2646 2646 Size: 5056 KpnI G_GTAC'C Cuts at: 2381 2381 Size: 5056 MfeI C'AATT_G Cuts at: 2655 2655 Size: 5056 MluI A'CGCG_T Cuts at: 4932 4932 Size: 5056 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4170 4354 1305 Size: 2865 184 2007 Fragments arranged by size: 2865 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3234 Fragments arranged by size: 3234 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3014 3373 3532 518 Size: 648 1848 359 159 2042 Fragments arranged by size: 2042 1848 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2334 2472 3149 4297 4542 1606 Size: 728 138 677 1148 245 2120 Fragments arranged by size: 2120 1148 728 677 245 138 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4504 NdeI CA'TA_TG Cuts at: 387 387 Size: 5056 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5056 NotI GC'GGCC_GC Cuts at: 2503 2503 Size: 5056 NspI r_CATG'y Cuts at: 1132 1229 2414 3959 1132 Size: 97 1185 1545 2229 Fragments arranged by size: 2229 1545 1185 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5056 PciI A'CATG_T Cuts at: 3955 3955 Size: 5056 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 2035 1108 Size: 902 25 4129 Fragments arranged by size: 4129 902 25 PmeI GTTT'AAAC Cuts at: 2360 2360 Size: 5056 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3125 3157 4638 4670 3125 Size: 32 1481 32 3511 Fragments arranged by size: 3511 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5056 PsiI TTA'TAA Cuts at: 2626 2626 Size: 5056 PstI C_TGCA'G Cuts at: 830 2408 830 Size: 1578 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5056 SalI G'TCGA_C Cuts at: 1936 2398 1936 Size: 462 4594 SbfI CC_TGCA'GG Cuts at: 2408 2408 Size: 5056 ScaI AGT'ACT Cuts at: 2150 3304 5016 2150 Size: 1154 1712 2190 Fragments arranged by size: 2190 1712 1154 SfcI C'TryA_G Cuts at: 826 1045 2329 2404 3539 4220 4411 826 Size: 219 1284 75 1135 681 191 1471 Fragments arranged by size: 1471 1284 1135 681 219 191 75 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5056 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5056 SmaI CCC'GGG Cuts at: 1872 2383 1872 Size: 511 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3160 4061 4323 820 Size: 197 926 1217 901 262 1553 Fragments arranged by size: 1553 1217 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2847 493 Size: 2354 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5056 SphI G_CATG'C Cuts at: 1132 2414 1132 Size: 1282 3774 SspI AAT'ATT Cuts at: 5 52 2980 5 Size: 47 2928 2081 Fragments arranged by size: 2928 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5056 StyI C'CwwG_G Cuts at: 513 1065 2495 513 Size: 552 1430 3074 Fragments arranged by size: 3074 1430 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3083 3100 3253 3438 3883 325 Size: 1255 1503 17 153 185 445 1498 Fragments arranged by size: 1503 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2148 3302 5014 Size: 274 80 33 51 1443 171 1154 1712 Cuts at: 5014 96 Size: 138 Fragments arranged by size: 1712 1443 1154 274 171 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5016 5048 5016 Size: 32 5024 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5056 XbaI T'CTAG_A Cuts at: 2392 2392 Size: 5056 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2272 2272 Size: 5056 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5056 XmaI C'CCGG_G Cuts at: 1870 2381 1870 Size: 511 4545 XmnI GAAnn'nnTTC Cuts at: 3185 3185 Size: 5056 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2864 276 Size: 53 83 186 936 1330 2468 Fragments arranged by size: 2468 1330 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI