(Circular) (Six-base) MAPSORT of: pFN21AB8310.seq Check: 2689 from: 1 to: 5914 pFN21AB8310 5914 bp With 182 enzymes: SgfI * February 27, 2009 14:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3724 278 Size: 53 83 186 936 2188 2468 Fragments arranged by size: 2468 2188 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2622 3257 1937 Size: 685 635 4594 Fragments arranged by size: 4594 685 635 Acc65I G'GTAC_C Cuts at: 3235 3235 Size: 5914 AclI AA'CG_TT Cuts at: 1563 4041 4414 1563 Size: 2478 373 3063 Fragments arranged by size: 3063 2478 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2654 3977 5361 5629 1632 Size: 1022 1323 1384 268 1917 Fragments arranged by size: 1917 1384 1323 1022 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5717 AflIII A'CryG_T Cuts at: 1203 2074 4813 5790 1203 Size: 871 2739 977 1327 Fragments arranged by size: 2739 1327 977 871 AgeI A'CCGG_T Cuts at: 4745 4745 Size: 5914 AhdI GACnn_n'nnGTC Cuts at: 4643 4643 Size: 5914 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5914 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2718 1184 Size: 1534 4380 AlwNI CAG_nnn'CTG Cuts at: 2039 2308 3657 5229 2039 Size: 269 1349 1572 2724 Fragments arranged by size: 2724 1572 1349 269 ApaLI G'TGCA_C Cuts at: 3971 5127 3971 Size: 1156 4758 ApoI r'AATT_y Cuts at: 1493 2158 2966 3038 3160 3223 3449 3601 5710 Size: 665 808 72 122 63 226 152 2109 Cuts at: 5710 1493 Size: 1697 Fragments arranged by size: 2109 1697 808 665 226 152 122 72 63 AseI AT'TA_AT Cuts at: 160 1032 4468 160 Size: 872 3436 1606 Fragments arranged by size: 3436 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5914 AvaI C'yCGr_G Cuts at: 1870 1943 3239 1870 Size: 73 1296 4545 Fragments arranged by size: 4545 1296 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2238 2271 2238 Size: 33 5881 BamHI G'GATC_C Cuts at: 3244 3611 3244 Size: 367 5547 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2317 2876 3235 4690 618 Size: 325 217 627 530 559 359 1455 1842 Fragments arranged by size: 1842 1455 627 559 530 359 325 217 BanII G_rGCy'C Cuts at: 1391 3233 1391 Size: 1842 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2403 928 Size: 971 504 4439 Fragments arranged by size: 4439 971 504 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4105 4139 1903 Size: 34 2168 34 3678 Fragments arranged by size: 3678 2168 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3806 5016 3806 Size: 1210 4704 BclI T'GATC_A Cuts at: 1548 1548 Size: 5914 BfrBI ATG'CAT Cuts at: 2920 2920 Size: 5914 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4525 136 Size: 107 122 71 631 605 2853 1525 Fragments arranged by size: 2853 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5909 5909 Size: 5914 BlpI GC'TnA_GC Cuts at: 2136 2505 3331 2136 Size: 369 826 4719 Fragments arranged by size: 4719 826 369 Bme1580I G_kGCm'C Cuts at: 1790 3975 5131 1790 Size: 2185 1156 2573 Fragments arranged by size: 2573 2185 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5914 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3645 4603 442 Size: 416 53 83 399 24 2228 958 1753 Fragments arranged by size: 2228 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3204 1056 Size: 2148 3766 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2883 2883 Size: 5914 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2040 2185 2254 2531 2826 2962 4574 1239 Size: 801 145 69 277 295 136 1612 2579 Fragments arranged by size: 2579 1612 801 295 277 145 136 69 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4039 4904 5202 4039 Size: 865 298 4751 Fragments arranged by size: 4751 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3005 4577 882 Size: 617 1506 1572 2219 Fragments arranged by size: 2219 1572 1506 617 BsaAI yAC'GTr Cuts at: 493 1204 3705 493 Size: 711 2501 2702 Fragments arranged by size: 2702 2501 711 BsaBI GATnn'nnATC Cuts at: 5908 5908 Size: 5914 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3721 4103 4738 275 Size: 53 83 186 936 2188 382 635 1451 Fragments arranged by size: 2188 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4347 4745 5019 5166 5577 4347 Size: 398 274 147 411 4684 Fragments arranged by size: 4684 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5689 Fragments arranged by size: 5689 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2351 1186 Size: 1165 4749 BseYI C'CCAG_C Cuts at: 2166 3107 5117 5613 2166 Size: 941 2010 496 2467 Fragments arranged by size: 2467 2010 941 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5341 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3364 4125 5153 1540 Size: 275 186 1363 761 1028 2301 Fragments arranged by size: 2301 1363 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3975 4060 5131 3975 Size: 85 1071 4758 Fragments arranged by size: 4758 1071 85 BsmI GAATG_Cn' Cuts at: 3425 3518 3425 Size: 93 5821 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3233 3975 4060 5131 1391 Size: 399 1443 742 85 1071 2174 Fragments arranged by size: 2174 1443 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5577 5577 Size: 5914 BspHI T'CATG_A Cuts at: 3803 5533 3803 Size: 1730 4184 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2898 3269 844 Size: 2054 371 3489 Fragments arranged by size: 3489 2054 371 BsrBI CCG'CTC Cuts at: 1394 1823 3801 1394 Size: 429 1978 3507 Fragments arranged by size: 3507 1978 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4409 4583 65 Size: 1183 3161 174 1396 Fragments arranged by size: 3161 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2212 4558 4745 1700 Size: 110 402 2346 187 2869 Fragments arranged by size: 2869 2346 402 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5914 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5914 BssSI C'ACGA_G Cuts at: 2081 3974 4986 2081 Size: 1893 1012 3009 Fragments arranged by size: 3009 1893 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2308 794 Size: 1514 4400 BstBI TT'CG_AA Cuts at: 2750 4718 2750 Size: 1968 3946 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5914 BstYI r'GATC_y Cuts at: 1972 2935 3244 3611 3996 4013 5465 5551 5563 Size: 963 309 367 385 17 1452 86 12 Cuts at: 5563 5909 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 963 385 367 346 309 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2334 2334 Size: 5914 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4423 Fragments arranged by size: 4423 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5175 Fragments arranged by size: 5175 683 56 BtsI GCAGTG_nn' Cuts at: 2390 3430 4224 4244 2390 Size: 1040 794 20 4060 Fragments arranged by size: 4060 1040 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5914 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5879 DraI TTT'AAA Cuts at: 2500 3218 2500 Size: 718 5196 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4921 809 Size: 507 3605 1802 Fragments arranged by size: 3605 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2128 3361 4250 Size: 54 1425 50 275 18 298 1233 889 Cuts at: 4250 5879 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1233 889 298 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3361 1537 Size: 275 1549 4090 Fragments arranged by size: 4090 1549 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2536 2747 3844 1353 Size: 1183 211 1097 3423 Fragments arranged by size: 3423 1183 1097 211 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4501 4875 5021 387 Size: 1064 138 2912 374 146 1280 Fragments arranged by size: 2912 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2040 2185 2254 2531 2654 2826 2962 Size: 393 408 145 69 277 123 172 136 Cuts at: 2962 3977 4574 5361 5629 1239 Size: 1015 597 787 268 1524 Fragments arranged by size: 1524 1015 787 597 408 393 277 268 172 145 136 123 69 EcoO109I rG'GnC_Cy Cuts at: 2036 3076 2036 Size: 1040 4874 EcoRI G'AATT_C Cuts at: 3038 3223 3601 3038 Size: 185 378 5351 Fragments arranged by size: 5351 378 185 FspI TGC'GCA Cuts at: 4420 4420 Size: 5914 HaeII r_GCGC'y Cuts at: 1678 5061 1678 Size: 3383 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2896 2928 4555 4587 4629 Size: 32 135 32 1324 32 1627 32 42 Cuts at: 4629 4661 1373 Size: 32 2626 Fragments arranged by size: 2626 1627 1324 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3258 3504 669 Size: 988 281 1320 246 3079 Fragments arranged by size: 3079 1320 988 281 246 HindIII A'AGCT_T Cuts at: 748 2192 748 Size: 1444 4470 HpaI GTT'AAC Cuts at: 3504 3504 Size: 5914 KpnI G_GTAC'C Cuts at: 3239 3239 Size: 5914 MfeI C'AATT_G Cuts at: 2237 3513 2237 Size: 1276 4638 MluI A'CGCG_T Cuts at: 5790 5790 Size: 5914 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2041 2894 5028 5212 1305 Size: 736 853 2134 184 2007 Fragments arranged by size: 2134 2007 853 736 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2130 10 Size: 54 1425 343 298 3794 Fragments arranged by size: 3794 1425 343 298 54 MslI CAynn'nnrTG Cuts at: 518 1166 3872 4231 4390 518 Size: 648 2706 359 159 2042 Fragments arranged by size: 2706 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2140 2509 2804 3330 4007 5155 5400 1606 Size: 534 369 295 526 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 534 526 369 295 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4423 Fragments arranged by size: 4423 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5914 NheI G'CTAG_C Cuts at: 1052 3200 1052 Size: 2148 3766 NotI GC'GGCC_GC Cuts at: 3361 3361 Size: 5914 NsiI A_TGCA'T Cuts at: 2922 2922 Size: 5914 NspI r_CATG'y Cuts at: 1132 1229 2846 3272 4817 1132 Size: 97 1617 426 1545 2229 Fragments arranged by size: 2229 1617 1545 426 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5914 PciI A'CATG_T Cuts at: 4813 4813 Size: 5914 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5914 PfoI T'CCnGG_A Cuts at: 2855 2855 Size: 5914 PmeI GTTT'AAAC Cuts at: 3218 3218 Size: 5914 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3983 4015 5496 5528 3983 Size: 32 1481 32 4369 Fragments arranged by size: 4369 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2036 3076 2036 Size: 1040 4874 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5914 PsiI TTA'TAA Cuts at: 3484 3484 Size: 5914 PstI C_TGCA'G Cuts at: 830 3266 830 Size: 2436 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5914 PvuII CAG'CTG Cuts at: 2509 2804 2509 Size: 295 5619 SalI G'TCGA_C Cuts at: 1936 3256 1936 Size: 1320 4594 SbfI CC_TGCA'GG Cuts at: 3266 3266 Size: 5914 ScaI AGT'ACT Cuts at: 3135 4162 5874 3135 Size: 1027 1712 3175 Fragments arranged by size: 3175 1712 1027 SfcI C'TryA_G Cuts at: 826 1045 2049 3262 4397 5078 5269 826 Size: 219 1004 1213 1135 681 191 1471 Fragments arranged by size: 1471 1213 1135 1004 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5914 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5914 SmaI CCC'GGG Cuts at: 1872 3241 1872 Size: 1369 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4018 4919 5181 820 Size: 197 926 2075 901 262 1553 Fragments arranged by size: 2075 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3705 493 Size: 3212 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5914 SphI G_CATG'C Cuts at: 1132 3272 1132 Size: 2140 3774 SspI AAT'ATT Cuts at: 5 52 2581 3838 5 Size: 47 2529 1257 2081 Fragments arranged by size: 2529 2081 1257 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5914 StyI C'CwwG_G Cuts at: 513 1065 2004 2947 3353 513 Size: 552 939 943 406 3074 Fragments arranged by size: 3074 943 939 552 406 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2025 2303 2820 3941 3958 4111 4296 Size: 1255 445 278 517 1121 17 153 185 Cuts at: 4296 4741 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1121 517 445 445 278 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2839 3133 4160 Size: 274 80 33 51 1443 862 294 1027 Cuts at: 4160 5872 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1027 862 294 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5874 5906 5874 Size: 32 5882 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5914 XbaI T'CTAG_A Cuts at: 3250 3250 Size: 5914 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5914 XmaI C'CCGG_G Cuts at: 1870 3239 1870 Size: 1369 4545 XmnI GAAnn'nnTTC Cuts at: 4043 4043 Size: 5914 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3722 276 Size: 53 83 186 936 2188 2468 Fragments arranged by size: 2468 2188 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI