################################## # NO CUT ENZYMES ( 106 enzymes) # ################################## AatII Acc65I AclI AcsI AflII AgeI AhdI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BbeI BbsI BcgI BciVI BfaI BfrI BglI BlnI BpuAI BsaBI Bsp1407I BspDI BsrBI BsrGI BsrI BssHII Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraI Eam1105I EarI EcoO65I EcoRI EcoRV EcoT22I FspI HindIII HpaI KasI KpnI Ksp632I KspI MaeI MamI MfeI MluI MluNI MscI MunI NarI NcoI NdeI NheI NotI NruI NsiI NspV PacI PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tsp509I Tth111I XbaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccIII 1010 TCCGGA T^CCGGA AcyI 1180 GACGCC G[GA]^CG[CT]C Alw44I 1543 GTGCAC G^TGCAC AocI 1411 CCTGAGG CC^T[ACGT]AGG Aor51HI 641 AGCGCT AGC^GCT ApaLI 1543 GTGCAC G^TGCAC AvaI 1108 CTCGAG C^[CT]CG[GA]G BbrPI 469 CACGTG CAC^GTG BbvCI 1089 CCTCAGC CCTCAGC(-5/-2) BglII 1004 AGATCT A^GATCT BlpI 1513 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1513 GCTGAGC GC^T[ACGT]AGC BsaHI 1180 GACGCC G[GA]^CG[CT]C BsaWI 1010 TCCGGA [AT]^CCGG[AT] BseAI 1010 TCCGGA T^CCGGA BsiWI 614 CGTACG C^GTACG BsmFI 996 GTCCC GGGAC(10/14) BsoBI 1108 CTCGAG C^[CT]CG[GA]G BspEI 1010 TCCGGA T^CCGGA BspHI 1451 TCATGA T^CATGA BssSI 676 CACGAG CACGAG(-5/-1) Bsu36I 1411 CCTGAGG CC^T[ACGT]AGG CelII 1513 GCTGAGC GC^T[ACGT]AGC DraIII 1621 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1653 GACTGTGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 162 CGGCCG C^GGCCG EclXI 162 CGGCCG C^GGCCG Eco47III 641 AGCGCT AGC^GCT Eco52I 162 CGGCCG C^GGCCG Eco81I 1411 CCTGAGG CC^T[ACGT]AGG FseI 30 GGCCGGCC GGCCGG^CC HaeII 641 AGCGCT [GA]GCGC^[CT] Hin1I 1180 GACGCC G[GA]^CG[CT]C HincII 1297 GTCAAC GT[CT]^[GA]AC HindII 1297 GTCAAC GT[CT]^[GA]AC MroI 1010 TCCGGA T^CCGGA MseI 497 TTAA T^TAA PaeR7I 1108 CTCGAG C^TCGAG PmaCI 469 CACGTG CAC^GTG PmlI 469 CACGTG CAC^GTG PpuMI 1694 AGGACCC [GA]G^G[AT]CC[CT] PstI 1000 CTGCAG CTGCA^G RcaI 1451 TCATGA T^CATGA Tru9I 497 TTAA T^TAA XcmI 1706 CCAAGGTGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 1108 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AfaI 615 GTAC GT^AC 1155 GTAC AflIII 893 ACATGT A^C[GA][CT]GT 1157 ACATGT AlwI 867 GATCC GGATC(4/5) 1086 GATCC BsaAI 469 CACGTG [CT]AC^GT[GA] 612 CACGTA BseRI 1669 CTCCTC GAGGAG(10/8) 1741 GAGGAG BsmBI 1324 GAGACG CGTCTC(1/5) 1573 GAGACG BsmI 101 GAATGC GAATGC(1/-1) 977 GAATGC BspLU11I 893 ACATGT A^CATGT 1157 ACATGT BsrDI 69 CATTGC GCAATG(2/0) 1580 CATTGC BsrFI 31 GCCGGC [GA]^CCGG[CT] 1311 GCCGGC BstXI 302 CCATCTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1250 CCAAGAAGATGG Cfr10I 31 GCCGGC [GA]^CCGG[CT] 1311 GCCGGC DsaI 683 CCGTGG C^C[GA][CT]GG 803 CCACGG EcoNI 1668 CCTCCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1782 CCTCCCCCAGG NaeI 31 GCCGGC GCC^GGC 1311 GCCGGC NgoMIV 31 GCCGGC G^CCGGC 1311 GCCGGC PflMI 36 CCAGGACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 468 CCACGTGCTGG PvuII 1198 CAGCTG CAG^CTG 1522 CAGCTG RsaI 615 GTAC GT^AC 1155 GTAC SacI 739 GAGCTC GAGCT^C 1288 GAGCTC SfcI 1000 CTGCAG C^T[GA][CT]AG 1590 CTACAG SmlI 1108 CTCGAG C^T[CT][GA]AG 1600 CTCAAG StuI 452 AGGCCT AGG^CCT 917 AGGCCT TspRI 217 CACTG CA[GC]TG[ACGT][ACGT]^ 1621 CACTG Van91I 36 CCAGGACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 468 CCACGTGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 81 enzymes) # ######################################### AccI AccII AciI AluI AlwNI AspHI AvaII BanI BanII BbvI BclI BcnI BmyI BpmI BsaI BsaJI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI Bsp1286I BspMI BssKI BstAPI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MflI MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI Sau3AI Sau96I ScrFI SfaNI StyI TaqI TseI Tsp45I TthHB8I XhoII