(Linear) (Six-base) MAPSORT of: KIBB8303.seq Check: 429 from: 1 to: 1800 KIBB8303.orf With 182 enzymes: SgfI * March 3, 2008 11:04 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1116 1137 1543 1556 1800 Size: 1116 21 406 13 244 Fragments arranged by size: 1116 406 244 21 13 AccI GT'mk_AC Cuts at: 0 335 521 1589 1800 Size: 335 186 1068 211 Fragments arranged by size: 1068 335 211 186 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 222 685 802 1288 1402 1633 1792 1800 Size: 222 463 117 486 114 231 159 8 Fragments arranged by size: 486 463 231 222 159 117 114 8 AfeI AGC'GCT Cuts at: 0 643 1800 Size: 643 1157 AflIII A'CryG_T Cuts at: 0 893 1157 1800 Size: 893 264 643 Fragments arranged by size: 893 643 264 AleI CACnn'nnGTG Cuts at: 0 1708 1800 Size: 1708 92 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1726 1800 Size: 1726 74 AlwNI CAG_nnn'CTG Cuts at: 0 514 999 1131 1404 1800 Size: 514 485 132 273 396 Fragments arranged by size: 514 485 396 273 132 ApaLI G'TGCA_C Cuts at: 0 1543 1800 Size: 1543 257 AvaI C'yCGr_G Cuts at: 0 1108 1800 Size: 1108 692 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 700 733 1800 Size: 700 33 1067 Fragments arranged by size: 1067 700 33 BanI G'GyrC_C Cuts at: 0 786 1069 1441 1702 1800 Size: 786 283 372 261 98 Fragments arranged by size: 786 372 283 261 98 BanII G_rGCy'C Cuts at: 0 508 743 1292 1800 Size: 508 235 549 508 Fragments arranged by size: 549 508 508 235 BbvCI CC'TCA_GC Cuts at: 0 1090 1800 Size: 1090 710 BclI T'GATC_A Cuts at: 0 191 374 1448 1800 Size: 191 183 1074 352 Fragments arranged by size: 1074 352 191 183 BglII A'GATC_T Cuts at: 0 1004 1800 Size: 1004 796 BlpI GC'TnA_GC Cuts at: 0 1514 1800 Size: 1514 286 Bme1580I G_kGCm'C Cuts at: 0 228 791 1072 1547 1800 Size: 228 563 281 475 253 Fragments arranged by size: 563 475 281 253 228 BmgBI CAC'GTC Cuts at: 0 42 1800 Size: 42 1758 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 331 463 775 1153 1657 1800 Size: 331 132 312 378 504 143 Fragments arranged by size: 504 378 331 312 143 132 Bpu10I CC'TnA_GC Cuts at: 0 1090 1800 Size: 1090 710 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1585 1800 Size: 1585 215 BsaI GGTCTCn'nnnn_ Cuts at: 0 69 673 751 1725 1800 Size: 69 604 78 974 75 Fragments arranged by size: 974 604 78 75 69 BsaAI yAC'GTr Cuts at: 0 471 614 1800 Size: 471 143 1186 Fragments arranged by size: 1186 471 143 BsaHI Gr'CG_yC Cuts at: 0 1181 1800 Size: 1181 619 BsaWI w'CCGG_w Cuts at: 0 1010 1800 Size: 1010 790 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1144 1174 1800 Size: 1144 30 626 Fragments arranged by size: 1144 626 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1660 1756 1800 Size: 1660 96 44 Fragments arranged by size: 1660 96 44 BseYI C'CCAG_C Cuts at: 0 474 723 988 1348 1371 1503 1746 1795 Size: 474 249 265 360 23 132 243 49 Cuts at: 1795 1800 Size: 5 Fragments arranged by size: 474 360 265 249 243 132 49 23 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 904 967 1084 1420 1648 1800 Size: 904 63 117 336 228 152 Fragments arranged by size: 904 336 228 152 117 63 BsiEI CG_ry'CG Cuts at: 0 126 165 927 1800 Size: 126 39 762 873 Fragments arranged by size: 873 762 126 39 BsiHKAI G_wGCw'C Cuts at: 0 743 1292 1520 1547 1800 Size: 743 549 228 27 253 Fragments arranged by size: 743 549 253 228 27 BsiWI C'GTAC_G Cuts at: 0 614 1800 Size: 614 1186 BsmI GAATG_Cn' Cuts at: 0 107 983 1800 Size: 107 876 817 Fragments arranged by size: 876 817 107 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1318 1567 1800 Size: 1318 249 233 Fragments arranged by size: 1318 249 233 Bsp1286I G_dGCh'C Cuts at: 0 228 508 743 791 1072 1292 1520 1547 Size: 228 280 235 48 281 220 228 27 Cuts at: 1547 1800 Size: 253 Fragments arranged by size: 281 280 253 235 228 228 220 48 27 BspEI T'CCGG_A Cuts at: 0 1010 1800 Size: 1010 790 BspHI T'CATG_A Cuts at: 0 1451 1800 Size: 1451 349 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 602 1116 1137 1543 1556 1800 Size: 602 514 21 406 13 244 Fragments arranged by size: 602 514 406 244 21 13 BsrDI GCAATG_nn' Cuts at: 0 68 1579 1800 Size: 68 1511 221 Fragments arranged by size: 1511 221 68 BsrFI r'CCGG_y Cuts at: 0 31 1311 1800 Size: 31 1280 489 Fragments arranged by size: 1280 489 31 BssSI C'ACGA_G Cuts at: 0 676 1800 Size: 676 1124 BstAPI GCAn_nnn'nTGC Cuts at: 0 999 1404 1524 1800 Size: 999 405 120 276 Fragments arranged by size: 999 405 276 120 BstXI CCAn_nnnn'nTGG Cuts at: 0 309 1257 1800 Size: 309 948 543 Fragments arranged by size: 948 543 309 BstYI r'GATC_y Cuts at: 0 866 1004 1085 1800 Size: 866 138 81 715 Fragments arranged by size: 866 715 138 81 Bsu36I CC'TnA_GG Cuts at: 0 1412 1800 Size: 1412 388 BtgI C'CryG_G Cuts at: 0 683 803 1800 Size: 683 120 997 Fragments arranged by size: 997 683 120 BtsI GCAGTG_nn' Cuts at: 0 216 1800 Size: 216 1584 DraIII CAC_nnn'GTG Cuts at: 0 1626 1800 Size: 1626 174 DrdI GACnn_nn'nnGTC Cuts at: 0 1659 1800 Size: 1659 141 EaeI y'GGCC_r Cuts at: 0 33 162 1077 1313 1800 Size: 33 129 915 236 487 Fragments arranged by size: 915 487 236 129 33 EagI C'GGCC_G Cuts at: 0 162 1800 Size: 162 1638 EcoICRI GAG'CTC Cuts at: 0 741 1290 1800 Size: 741 549 510 Fragments arranged by size: 741 549 510 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 222 331 463 685 775 802 1153 1288 Size: 222 109 132 222 90 27 351 135 Cuts at: 1288 1402 1633 1657 1792 1800 Size: 114 231 24 135 8 Fragments arranged by size: 351 231 222 222 135 135 132 114 109 90 27 24 8 EcoNI CCTnn'n_nnAGG Cuts at: 0 1672 1786 1800 Size: 1672 114 14 Fragments arranged by size: 1672 114 14 EcoO109I rG'GnC_Cy Cuts at: 0 1695 1779 1791 1800 Size: 1695 84 12 9 Fragments arranged by size: 1695 84 12 9 FseI GG_CCGG'CC Cuts at: 0 35 1800 Size: 35 1765 HaeII r_GCGC'y Cuts at: 0 645 1800 Size: 645 1155 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1144 1176 1800 Size: 1144 32 624 Fragments arranged by size: 1144 624 32 HincII GTy'rAC Cuts at: 0 1299 1800 Size: 1299 501 MslI CAynn'nnrTG Cuts at: 0 271 1550 1708 1800 Size: 271 1279 158 92 Fragments arranged by size: 1279 271 158 92 MspA1I CmG'CkG Cuts at: 0 1094 1101 1200 1524 1800 Size: 1094 7 99 324 276 Fragments arranged by size: 1094 324 276 99 7 NaeI GCC'GGC Cuts at: 0 33 1313 1800 Size: 33 1280 487 Fragments arranged by size: 1280 487 33 NgoMIV G'CCGG_C Cuts at: 0 31 1311 1800 Size: 31 1280 489 Fragments arranged by size: 1280 489 31 NspI r_CATG'y Cuts at: 0 897 1161 1311 1800 Size: 897 264 150 489 Fragments arranged by size: 897 489 264 150 PciI A'CATG_T Cuts at: 0 893 1157 1800 Size: 893 264 643 Fragments arranged by size: 893 643 264 PflMI CCAn_nnn'nTGG Cuts at: 0 42 474 1800 Size: 42 432 1326 Fragments arranged by size: 1326 432 42 PfoI T'CCnGG_A Cuts at: 0 179 752 1637 1723 1800 Size: 179 573 885 86 77 Fragments arranged by size: 885 573 179 86 77 PmlI CAC'GTG Cuts at: 0 471 1800 Size: 471 1329 PpuMI rG'GwC_Cy Cuts at: 0 1695 1800 Size: 1695 105 PsiI TTA'TAA Cuts at: 0 536 1800 Size: 536 1264 PstI C_TGCA'G Cuts at: 0 1004 1800 Size: 1004 796 PvuII CAG'CTG Cuts at: 0 1200 1524 1800 Size: 1200 324 276 Fragments arranged by size: 1200 324 276 SacI G_AGCT'C Cuts at: 0 743 1292 1800 Size: 743 549 508 Fragments arranged by size: 743 549 508 SfcI C'TryA_G Cuts at: 0 1000 1590 1800 Size: 1000 590 210 Fragments arranged by size: 1000 590 210 SmlI C'TyrA_G Cuts at: 0 1108 1600 1800 Size: 1108 492 200 Fragments arranged by size: 1108 492 200 StuI AGG'CCT Cuts at: 0 454 919 1800 Size: 454 465 881 Fragments arranged by size: 881 465 454 StyI C'CwwG_G Cuts at: 0 23 1184 1706 1800 Size: 23 1161 522 94 Fragments arranged by size: 1161 522 94 23 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 140 169 301 566 829 966 1800 Size: 140 29 132 265 263 137 834 Fragments arranged by size: 834 265 263 140 137 132 29 TatI w'GTAC_w Cuts at: 0 1154 1800 Size: 1154 646 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1713 1800 Size: 1713 87 XhoI C'TCGA_G Cuts at: 0 1108 1800 Size: 1108 692 Enzymes that do cut: AarI AccI AcuI AfeI AflIII AleI AlfI AlwNI ApaLI AvaI BaeI BanI BanII BbvCI BclI BglII BlpI Bme1580I BmgBI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrDI BsrFI BssSI BstAPI BstXI BstYI Bsu36I BtgI BtsI DraIII DrdI EaeI EagI EcoICRI Eco57MI EcoNI EcoO109I FseI HaeII Hin4I HincII MslI MspA1I NaeI NgoMIV NspI PciI PflMI PfoI PmlI PpuMI PsiI PstI PvuII SacI SfcI SmlI StuI StyI TaqII TatI XcmI XhoI Enzymes that do not cut: AatII Acc65I AclI AflII AgeI AhdI AloI ApaI ApoI AscI AseI AsiSI AvrII BamHI BbeI BbsI BcgI BciVI BfrBI BglI BmrI BmtI BplI BsaBI BsrBI BsrGI BssHII BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI EarI EciI EcoRI EcoRV FalI FspI FspAI HindIII HpaI KasI KpnI MfeI MluI MmeI MscI NarI NcoI NdeI NheI NotI NruI NsiI PacI PasI PmeI PpiI PshAI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI TstI Tth111I XbaI XmaI XmnI ZraI