################################## # NO CUT ENZYMES ( 121 enzymes) # ################################## Acc65I AccIII AclI AcsI AflII AflIII AgeI AhdI AocI Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AvaI AviII AvrII BamHI BbrPI BbvCI BcgI BciVI BfaI BfrI BlnI BlpI Bpu1102I BsaAI BseAI BsiWI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HincII HindII HindIII HpaI KpnI KspI MaeI MfeI MluI MroI MseI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tru9I Tsp509I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 30 enzymes) # ################################### AccI 1285 GTCTAC GT^[AC][GT]AC Alw44I 715 GTGCAC G^TGCAC AlwNI 274 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 715 GTGCAC G^TGCAC BbeI 960 GGCGCC GGCGC^C BbsI 246 GAAGAC GAAGAC(2/6) BglII 602 AGATCT A^GATCT BpuAI 246 GAAGAC GAAGAC(2/6) BsaBI 576 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 1118 TCCGGT [AT]^CCGG[AT] BsiEI 207 CGGTCG CG[GA][CT]^CG BsrI 90 ACTGG ACTGG(1/-1) BssSI 733 CACGAG CACGAG(-5/-1) BstAPI 1115 GCATCCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC DraIII 718 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG EcoNI 543 CCTGACCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG KasI 960 GGCGCC G^GCGCC MamI 576 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NaeI 310 GCCGGC GCC^GGC NarI 960 GGCGCC GG^CGCC NgoMIV 310 GCCGGC G^CCGGC NspI 905 ACATGC [GA]CATG^[CT] PflMI 903 CCACATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SacI 691 GAGCTC GAGCT^C SapI 777 GCTCTTC GCTCTTC(1/4) SfcI 1083 CTACAG C^T[GA][CT]AG SfoI 960 GGCGCC GGC^GCC SmlI 487 CTCAAG C^T[CT][GA]AG StuI 362 AGGCCT AGG^CCT Van91I 903 CCACATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AatII 742 GACGTC GACGT^C 1188 GACGTC ApaI 282 GGGCCC GGGCC^C 941 GGGCCC AspHI 691 GAGCTC G[AT]GC[AT]^C 715 GTGCAC AspI 553 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 895 GACGAGGTC BalI 228 TGGCCA TGG^CCA 977 TGGCCA BbvI 1059 GCTGC GCAGC(8/12) 1222 GCTGC BclI 575 TGATCA T^GATCA 971 TGATCA BsaI 505 GAGACC GGTCTC(1/5) 1372 GAGACC BseRI 397 GAGGAG GAGGAG(10/8) 853 GAGGAG BsiHKAI 691 GAGCTC G[AT]GC[AT]^C 715 GTGCAC BspMI 1046 ACCTGC ACCTGC(4/8) 1263 GCAGGT BsrBI 618 CCGCTC CCGCTC(-3/-3) 1311 CCGCTC DdeI 783 CTCAG C^T[ACGT]AG 842 CTGAG FbaI 575 TGATCA T^GATCA 971 TGATCA HphI 787 GGTGA GGTGA(8/7) 578 TCACC MluNI 228 TGGCCA TGG^CCA 977 TGGCCA MscI 228 TGGCCA TGG^CCA 977 TGGCCA MspA1I 145 CAGCTG C[AC]G^C[GT]G 1220 CAGCTG NcoI 260 CCATGG C^CATGG 1376 CCATGG PflFI 553 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 895 GACGAGGTC PleI 538 GAGTC GAGTC(4/5) 1306 GAGTC PvuII 145 CAGCTG CAG^CTG 1220 CAGCTG TfiI 62 GAATC G^A[AT]TC 1001 GAATC TseI 1059 GCTGC G^C[AT]GC 1222 GCTGC Tth111I 553 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 895 GACGAGGTC XcmI 509 CCATCTACGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1040 CCATAGACCTGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 81 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AvaII BanI BanII BcnI BglI BmyI BpmI BsaHI BsaJI BsgI BsiYI BslI BsmAI BsmBI BsmFI Bsp1286I BsrFI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII ItaI Ksp632I MaeII MaeIII MboI MboII MflI MnlI MslI MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI StyI TaqI Tsp45I TspRI TthHB8I XhoII