################################## # NO CUT ENZYMES ( 148 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AgeI AocI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BcnI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsiEI BsiWI BsmI BspDI BspEI BsrBI BsrDI BsrFI BssHII Bst1107I BstAPI BstBI BstUI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraIII DrdI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV EcoT22I FauI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HpaII KasI KpnI KspI MaeI MamI MflI MluI MluNI MroI MscI MspI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TaqI Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AfaI 279 GTAC GT^AC Alw44I 927 GTGCAC G^TGCAC AlwNI 1321 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 927 GTGCAC G^TGCAC AvaI 546 CCCGAG C^[CT]CG[GA]G BciVI 347 GTATCC GTATCC(6/5) BclI 48 TGATCA T^GATCA BglI 832 GCCTGCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 1327 CTGGAG CTGGAG(16/14) BsaI 670 GAGACC GGTCTC(1/5) BsgI 1179 CTGCAC GTGCAG(16/14) BsmBI 1330 GAGACG CGTCTC(1/5) BsoBI 546 CCCGAG C^[CT]CG[GA]G Bsp1407I 278 TGTACA T^GTACA BspHI 1134 TCATGA T^CATGA BspMI 1248 GCAGGT ACCTGC(4/8) BsrGI 278 TGTACA T^GTACA BssSI 1219 CACGAG CACGAG(-5/-1) BstXI 837 CCAGGCGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraI 181 TTTAAA TTT^AAA DsaI 665 CCATGG C^C[GA][CT]GG EaeI 648 CGGCCA [CT]^GGCC[GA] EarI 259 GAAGAG CTCTTC(1/4) FbaI 48 TGATCA T^GATCA HincII 313 GTTAAC GT[CT]^[GA]AC HindII 313 GTTAAC GT[CT]^[GA]AC HpaI 313 GTTAAC GTT^AAC Ksp632I 259 GAAGAG CTCTTC(1/4) MaeII 853 ACGT A^CGT MfeI 639 CAATTG C^AATTG MspA1I 1324 CAGCTG C[AC]G^C[GT]G MunI 639 CAATTG C^AATTG NcoI 665 CCATGG C^CATGG PleI 549 GAGTC GAGTC(4/5) PstI 168 CTGCAG CTGCA^G PvuII 1324 CAGCTG CAG^CTG RcaI 1134 TCATGA T^CATGA RsaI 279 GTAC GT^AC SspBI 278 TGTACA T^GTACA ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AcsI 100 GAATTT [GA]^AATT[CT] 136 AAATTC AflIII 275 ACATGT A^C[GA][CT]GT 1091 ACATGT AhdI 559 GACGGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 688 GACTTGGGGTC ApoI 100 GAATTT [GA]^AATT[CT] 136 AAATTC AspEI 559 GACGGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 688 GACTTGGGGTC AspHI 143 GAGCAC G[AT]GC[AT]^C 927 GTGCAC AvaII 264 GGTCC G^G[AT]CC 695 GGTCC BbsI 849 GAAGAC GAAGAC(2/6) 1142 GAAGAC BpuAI 849 GAAGAC GAAGAC(2/6) 1142 GAAGAC BsiHKAI 143 GAGCAC G[AT]GC[AT]^C 927 GTGCAC BsmFI 395 GTCCC GGGAC(10/14) 696 GTCCC BspLU11I 275 ACATGT A^CATGT 1091 ACATGT BstEII 811 GGTCACC G^GT[ACGT]ACC 1251 GGTTACC BstPI 811 GGTCACC G^GT[ACGT]ACC 1251 GGTTACC DdeI 374 CTCAG C^T[ACGT]AG 583 CTCAG DraII 263 AGGTCCT [GA]G^G[ACGT]CC[CT] 694 GGGTCCC Eam1105I 559 GACGGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 688 GACTTGGGGTC EcoO109I 263 AGGTCCT [GA]G^G[ACGT]CC[CT] 694 GGGTCCC EcoO65I 811 GGTCACC G^GT[ACGT]ACC 1251 GGTTACC HindIII 187 AAGCTT A^AGCTT 199 AAGCTT NspI 275 ACATGT [GA]CATG^[CT] 1091 ACATGT PpuMI 263 AGGTCCT [GA]G^G[AT]CC[CT] 694 GGGTCCC SfaNI 460 GATGC GCATC(5/9) 1201 GCATC ######################################### # 3 OR MORE CUT ENZYMES ( 48 enzymes) # ######################################### AciI AluI AlwI BbvI BmyI BsaJI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DpnII EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI MaeIII MboI MboII MnlI MseI MslI MvaI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfcI SmlI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI