(Circular) (Six-base) MAPSORT of: pFN21AB8279.seq Check: 1187 from: 1 to: 5923 pFN21AB8279 5923 bp With 182 enzymes: SgfI * February 27, 2009 14:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3733 278 Size: 53 83 186 936 2197 2468 Fragments arranged by size: 2468 2197 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2548 3266 1937 Size: 611 718 4594 Fragments arranged by size: 4594 718 611 Acc65I G'GTAC_C Cuts at: 3244 3244 Size: 5923 AclI AA'CG_TT Cuts at: 1563 4050 4423 1563 Size: 2487 373 3063 Fragments arranged by size: 3063 2487 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2433 2459 2462 2815 2899 3986 5370 5638 Size: 801 26 3 353 84 1087 1384 268 Cuts at: 5638 1632 Size: 1917 Fragments arranged by size: 1917 1384 1087 801 353 268 84 26 3 AfeI AGC'GCT Cuts at: 3053 3053 Size: 5923 AflII C'TTAA_G Cuts at: 820 1017 2033 3185 820 Size: 197 1016 1152 3558 Fragments arranged by size: 3558 1152 1016 197 AflIII A'CryG_T Cuts at: 1203 4822 5799 1203 Size: 3619 977 1327 Fragments arranged by size: 3619 1327 977 AgeI A'CCGG_T Cuts at: 4754 4754 Size: 5923 AhdI GACnn_n'nnGTC Cuts at: 2157 4652 2157 Size: 2495 3428 AleI CACnn'nnGTG Cuts at: 1166 3126 1166 Size: 1960 3963 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5923 AlwNI CAG_nnn'CTG Cuts at: 3666 5238 3666 Size: 1572 4351 ApaLI G'TGCA_C Cuts at: 3980 5136 3980 Size: 1156 4767 ApoI r'AATT_y Cuts at: 1493 3232 3458 3610 5719 1493 Size: 1739 226 152 2109 1697 Fragments arranged by size: 2109 1739 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4477 160 Size: 872 3445 1606 Fragments arranged by size: 3445 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5923 AvaI C'yCGr_G Cuts at: 1870 1943 2163 3248 1870 Size: 73 220 1085 4545 Fragments arranged by size: 4545 1085 220 73 BamHI G'GATC_C Cuts at: 3253 3620 3253 Size: 367 5556 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3244 4699 618 Size: 325 217 627 1457 1455 1842 Fragments arranged by size: 1842 1457 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2289 3242 1391 Size: 898 953 4072 Fragments arranged by size: 4072 953 898 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2061 928 Size: 971 162 4790 Fragments arranged by size: 4790 971 162 BbvCI CC'TCA_GC Cuts at: 3005 3005 Size: 5923 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4114 4148 1903 Size: 34 2177 34 3678 Fragments arranged by size: 3678 2177 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3027 3815 5025 3027 Size: 788 1210 3925 Fragments arranged by size: 3925 1210 788 BclI T'GATC_A Cuts at: 1548 1548 Size: 5923 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4534 136 Size: 107 122 71 631 605 2862 1525 Fragments arranged by size: 2862 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2350 5918 2350 Size: 3568 2355 BlpI GC'TnA_GC Cuts at: 3340 3340 Size: 5923 Bme1580I G_kGCm'C Cuts at: 1790 3984 5140 1790 Size: 2194 1156 2573 Fragments arranged by size: 2573 2194 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5923 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2799 3654 4612 Size: 416 53 83 399 24 1382 855 958 Cuts at: 4612 442 Size: 1753 Fragments arranged by size: 1753 1382 958 855 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5923 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2273 2276 2373 2727 2895 3063 3231 4583 Size: 1034 3 97 354 168 168 168 1352 Cuts at: 4583 1239 Size: 2579 Fragments arranged by size: 2579 1352 1034 354 168 168 168 97 3 Bpu10I CC'TnA_GC Cuts at: 2207 3005 2207 Size: 798 5125 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4048 4913 5211 4048 Size: 865 298 4760 Fragments arranged by size: 4760 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3211 4586 882 Size: 617 1712 1375 2219 Fragments arranged by size: 2219 1712 1375 617 BsaAI yAC'GTr Cuts at: 493 1204 3714 493 Size: 711 2510 2702 Fragments arranged by size: 2702 2510 711 BsaBI GATnn'nnATC Cuts at: 2503 5917 2503 Size: 3414 2509 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2716 3730 4112 4747 Size: 53 83 186 936 1183 1014 382 635 Cuts at: 4747 275 Size: 1451 Fragments arranged by size: 1451 1183 1014 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2314 4356 4754 5028 5175 5586 2314 Size: 2042 398 274 147 411 2651 Fragments arranged by size: 2651 2042 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5698 Fragments arranged by size: 5698 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2297 2342 2993 1186 Size: 1111 45 651 4116 Fragments arranged by size: 4116 1111 651 45 BseYI C'CCAG_C Cuts at: 5126 5622 5126 Size: 496 5427 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3166 3175 1163 Size: 573 1430 9 3911 Fragments arranged by size: 3911 1430 573 9 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3373 4134 5162 1540 Size: 275 186 1372 761 1028 2301 Fragments arranged by size: 2301 1372 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2289 2310 2421 2775 3984 4069 5140 2289 Size: 21 111 354 1209 85 1071 3072 Fragments arranged by size: 3072 1209 1071 354 111 85 21 BsmI GAATG_Cn' Cuts at: 2981 3097 3434 3527 2981 Size: 116 337 93 5377 Fragments arranged by size: 5377 337 116 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2707 2707 Size: 5923 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2289 2310 2421 2775 3242 3984 4069 Size: 399 499 21 111 354 467 742 85 Cuts at: 4069 5140 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 499 467 399 354 111 85 21 BspEI T'CCGG_A Cuts at: 2314 5586 2314 Size: 3272 2651 BspHI T'CATG_A Cuts at: 3812 5542 3812 Size: 1730 4193 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3278 844 Size: 2434 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3810 1394 Size: 429 1987 3507 Fragments arranged by size: 3507 1987 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2734 2932 4418 4592 65 Size: 1183 1486 198 1486 174 1396 Fragments arranged by size: 1486 1486 1396 1183 198 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2178 4567 4754 1700 Size: 110 368 2389 187 2869 Fragments arranged by size: 2869 2389 368 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5923 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5923 BssSI C'ACGA_G Cuts at: 3983 4995 3983 Size: 1012 4911 BstAPI GCAn_nnn'nTGC Cuts at: 794 3153 794 Size: 2359 3564 BstBI TT'CG_AA Cuts at: 4727 4727 Size: 5923 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5923 BstXI CCAn_nnnn'nTGG Cuts at: 2402 2402 Size: 5923 BstYI r'GATC_y Cuts at: 1972 2350 3253 3620 4005 4022 5474 5560 5572 Size: 378 903 367 385 17 1452 86 12 Cuts at: 5572 5918 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 903 385 378 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2549 2549 Size: 5923 Bsu36I CC'TnA_GG Cuts at: 2280 2280 Size: 5923 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4432 Fragments arranged by size: 4432 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5184 Fragments arranged by size: 5184 683 56 BtsI GCAGTG_nn' Cuts at: 3439 4233 4253 3439 Size: 794 20 5109 Fragments arranged by size: 5109 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5923 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2716 2751 2968 3003 529 Size: 35 2152 35 217 35 3449 Fragments arranged by size: 3449 2152 217 35 35 35 DraI TTT'AAA Cuts at: 3227 3227 Size: 5923 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4930 809 Size: 507 3614 1802 Fragments arranged by size: 3614 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2104 3370 4259 Size: 54 1425 50 275 18 274 1266 889 Cuts at: 4259 5888 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1266 889 275 274 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3370 1537 Size: 275 1558 4090 Fragments arranged by size: 4090 1558 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3853 1353 Size: 2500 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2878 4510 4884 5030 387 Size: 1064 138 1289 1632 374 146 1280 Fragments arranged by size: 1632 1289 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2287 2287 Size: 5923 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2273 2276 2373 2433 2459 2462 2727 Size: 393 641 3 97 60 26 3 265 Cuts at: 2727 2815 2895 2899 3063 3231 3986 4583 5370 Size: 88 80 4 164 168 755 597 787 Cuts at: 5370 5638 1239 Size: 268 1524 Fragments arranged by size: 1524 787 755 641 597 393 268 265 168 164 97 88 80 60 26 4 3 3 EcoNI CCTnn'n_nnAGG Cuts at: 2279 2279 Size: 5923 EcoRI G'AATT_C Cuts at: 3232 3610 3232 Size: 378 5545 FspI TGC'GCA Cuts at: 4429 4429 Size: 5923 HaeII r_GCGC'y Cuts at: 1678 3055 5070 1678 Size: 1377 2015 2531 Fragments arranged by size: 2531 2015 1377 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2143 2175 2555 2587 4564 Size: 32 135 32 571 32 380 32 1977 Cuts at: 4564 4596 4638 4670 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1977 571 380 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3267 3513 669 Size: 988 281 1329 246 3079 Fragments arranged by size: 3079 1329 988 281 246 HindIII A'AGCT_T Cuts at: 748 2743 748 Size: 1995 3928 HpaI GTT'AAC Cuts at: 3513 3513 Size: 5923 KpnI G_GTAC'C Cuts at: 3248 3248 Size: 5923 MfeI C'AATT_G Cuts at: 3522 3522 Size: 5923 MluI A'CGCG_T Cuts at: 5799 5799 Size: 5923 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5037 5221 1305 Size: 3732 184 2007 Fragments arranged by size: 3732 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2106 10 Size: 54 1425 343 274 3827 Fragments arranged by size: 3827 1425 343 274 54 MslI CAynn'nnrTG Cuts at: 518 1166 2400 2806 2974 3126 3881 4240 4399 Size: 648 1234 406 168 152 755 359 159 Cuts at: 4399 518 Size: 2042 Fragments arranged by size: 2042 1234 755 648 406 359 168 159 152 MspA1I CmG'CkG Cuts at: 1606 3339 4016 5164 5409 1606 Size: 1733 677 1148 245 2120 Fragments arranged by size: 2120 1733 1148 677 245 NaeI GCC'GGC Cuts at: 2180 2180 Size: 5923 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4432 Fragments arranged by size: 4432 939 552 NdeI CA'TA_TG Cuts at: 387 2803 2971 387 Size: 2416 168 3339 Fragments arranged by size: 3339 2416 168 NgoMIV G'CCGG_C Cuts at: 2178 2178 Size: 5923 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5923 NotI GC'GGCC_GC Cuts at: 3370 3370 Size: 5923 NspI r_CATG'y Cuts at: 1132 1229 3281 4826 1132 Size: 97 2052 1545 2229 Fragments arranged by size: 2229 2052 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5923 PciI A'CATG_T Cuts at: 4822 4822 Size: 5923 PflMI CCAn_nnn'nTGG Cuts at: 1108 2566 1108 Size: 1458 4465 PmeI GTTT'AAAC Cuts at: 3227 3227 Size: 5923 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3992 4024 5505 5537 3992 Size: 32 1481 32 4378 Fragments arranged by size: 4378 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5923 PsiI TTA'TAA Cuts at: 3493 3493 Size: 5923 PstI C_TGCA'G Cuts at: 830 3275 830 Size: 2445 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5923 SacI G_AGCT'C Cuts at: 2289 2289 Size: 5923 SalI G'TCGA_C Cuts at: 1936 3265 1936 Size: 1329 4594 SbfI CC_TGCA'GG Cuts at: 3275 3275 Size: 5923 ScaI AGT'ACT Cuts at: 2763 4171 5883 2763 Size: 1408 1712 2803 Fragments arranged by size: 2803 1712 1408 SfcI C'TryA_G Cuts at: 826 1045 2340 3271 4406 5087 5278 826 Size: 219 1295 931 1135 681 191 1471 Fragments arranged by size: 1471 1295 1135 931 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5923 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5923 SmaI CCC'GGG Cuts at: 1872 3250 1872 Size: 1378 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2033 3185 4027 4928 5190 820 Size: 197 926 90 1152 842 901 262 1553 Fragments arranged by size: 1553 1152 926 901 842 262 197 90 SnaBI TAC'GTA Cuts at: 493 3714 493 Size: 3221 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5923 SphI G_CATG'C Cuts at: 1132 3281 1132 Size: 2149 3774 SspI AAT'ATT Cuts at: 5 52 3847 5 Size: 47 3795 2081 Fragments arranged by size: 3795 2081 47 StuI AGG'CCT Cuts at: 1517 2294 2828 1517 Size: 777 534 4612 Fragments arranged by size: 4612 777 534 StyI C'CwwG_G Cuts at: 513 1065 2004 3362 513 Size: 552 939 1358 3074 Fragments arranged by size: 3074 1358 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2287 3950 3967 4120 4305 4750 325 Size: 1255 707 1663 17 153 185 445 1498 Fragments arranged by size: 1663 1498 1255 707 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2649 2761 4169 Size: 274 80 33 51 1443 672 112 1408 Cuts at: 4169 5881 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1408 672 274 138 112 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5883 5915 5883 Size: 32 5891 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5923 XbaI T'CTAG_A Cuts at: 3259 3259 Size: 5923 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2153 2318 2153 Size: 165 5758 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5923 XmaI C'CCGG_G Cuts at: 1870 3248 1870 Size: 1378 4545 XmnI GAAnn'nnTTC Cuts at: 2744 2828 2912 2996 4052 2744 Size: 84 84 84 1056 4615 Fragments arranged by size: 4615 1056 84 84 84 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3731 276 Size: 53 83 186 936 2197 2468 Fragments arranged by size: 2468 2197 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI DraIII EcoO109I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI