Restriction map for pFN21AB8275
 (Circular) (Six-base) MAPSORT of: pFN21AB8275.seq  Check: 9912  from: 1  to: 5623



pFN21AB8275  5623 bp

 With 182 enzymes: SgfI * 

                        February 27, 2009 14:41  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3433     278 
   Size:         53      83     186     936    1897    2468

  Fragments arranged by size: 

                2468    1897     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2376    2966    1937 
   Size:        439     590    4594

  Fragments arranged by size: 

                4594     590     439
 
Acc65I G'GTAC_C

Cuts at:   2944    2944 
   Size:       5623
 
AclI AA'CG_TT

Cuts at:   1563    3750    4123    1563 
   Size:       2187     373    3063

  Fragments arranged by size: 

                3063    2187     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2286    2363    2408    2607    3686    5070    5338    1632 
   Size:        654      77      45     199    1079    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1079     654     268     199      77      45
            
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5426
 
AflIII A'CryG_T

Cuts at:   1203    4522    5499    1203 
   Size:       3319     977    1327

  Fragments arranged by size: 

                3319    1327     977
 
AgeI A'CCGG_T

Cuts at:   4454    4454 
   Size:       5623
 
AhdI GACnn_n'nnGTC

Cuts at:   4352    4352 
   Size:       5623
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5623
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5623
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2003    2035    2003 
   Size:         32    5591
 
AlwNI CAG_nnn'CTG

Cuts at:   3366    4938    3366 
   Size:       1572    4051
 
ApaLI G'TGCA_C

Cuts at:   3680    4836    3680 
   Size:       1156    4467
 
ApoI r'AATT_y

Cuts at:   1493    2360    2553    2932    3158    3310    5419    1493 
   Size:        867     193     379     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     867     379     226     193     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4177     160 
   Size:        872    3145    1606

  Fragments arranged by size: 

                3145    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5623
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2948    1870 
   Size:         73    1005    4545

  Fragments arranged by size: 

                4545    1005      73
 
BamHI G'GATC_C

Cuts at:   2953    3320    2953 
   Size:        367    5256
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2168    2944    4399     618 
   Size:        325     217     627     381     776    1455    1842

  Fragments arranged by size: 

                1842    1455     776     627     381     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2643    2842    2942    1391 
   Size:       1252     199     100    4072

  Fragments arranged by size: 

                4072    1252     199     100
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4652
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3814    3848    1903 
   Size:         34    1877      34    3678

  Fragments arranged by size: 

                3678    1877      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   2893    3515    4725    2893 
   Size:        622    1210    3791

  Fragments arranged by size: 

                3791    1210     622
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5623
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2096    4234     136 
   Size:        107     122      71     631     605     424    2138    1525

  Fragments arranged by size: 

                2138    1525     631     605     424     122     107      71
            
 
BglII A'GATC_T

Cuts at:   2338    5618    2338 
   Size:       3280    2343
 
BlpI GC'TnA_GC

Cuts at:   3040    3040 
   Size:       5623
 
Bme1580I G_kGCm'C

Cuts at:   1790    3684    4840    1790 
   Size:       1894    1156    2573

  Fragments arranged by size: 

                2573    1894    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5623
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3354    4312     442 
   Size:        416      53      83     399      24    1937     958    1753

  Fragments arranged by size: 

                1937    1753     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5623
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2184    2559    4283    1239 
   Size:        945     375    1724    2579

  Fragments arranged by size: 

                2579    1724     945     375
 
Bpu10I CC'TnA_GC

Cuts at:   2315    2315 
   Size:       5623
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3748    4613    4911    3748 
   Size:        865     298    4460

  Fragments arranged by size: 

                4460     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4286     882 
   Size:        617    2787    2219

  Fragments arranged by size: 

                2787    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3414     493 
   Size:        711    2210    2702

  Fragments arranged by size: 

                2702    2210     711
 
BsaBI GATnn'nnATC

Cuts at:   5617    5617 
   Size:       5623
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2211    3430    3812    4447 
   Size:         53      83     186     936     678    1219     382     635

Cuts at:   4447     275 
   Size:       1451

  Fragments arranged by size: 

                1451    1219     936     678     635     382     186      83
                  53
 
BsaWI w'CCGG_w

Cuts at:   4056    4454    4728    4875    5286    4056 
   Size:        398     274     147     411    4393

  Fragments arranged by size: 

                4393     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2003    2033    2079    2109    1178 
   Size:         30     165      30     600      30      46      30    4692

  Fragments arranged by size: 

                4692     600     165      46      30      30      30      30
            
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2027    2239    2240    2835    1186 
   Size:        841     212       1     595    3974

  Fragments arranged by size: 

                3974     841     595     212       1
 
BseYI C'CCAG_C

Cuts at:   2319    4826    5322    2319 
   Size:       2507     496    2620

  Fragments arranged by size: 

                2620    2507     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2365    1163 
   Size:        573     629    4421

  Fragments arranged by size: 

                4421     629     573
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3073    3834    4862    1540 
   Size:        275     186    1072     761    1028    2301

  Fragments arranged by size: 

                2301    1072    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2643    2842    3684    3769    4840    2643 
   Size:        199     842      85    1071    3426

  Fragments arranged by size: 

                3426    1071     842     199      85
 
BsmI GAATG_Cn'

Cuts at:   3134    3227    3134 
   Size:         93    5530
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2643    2842    2942    3684    3769    4840    1391 
   Size:        399     853     199     100     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     853     742     399     199     100      85
            
 
BspEI T'CCGG_A

Cuts at:   5286    5286 
   Size:       5623
 
BspHI T'CATG_A

Cuts at:   3512    5242    3512 
   Size:       1730    3893
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2978     844 
   Size:       2134    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3510    1394 
   Size:        429    1687    3507

  Fragments arranged by size: 

                3507    1687     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4118    4292      65 
   Size:       1183    2870     174    1396

  Fragments arranged by size: 

                2870    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    2542    4267    4454    1700 
   Size:        110     732    1725     187    2869

  Fragments arranged by size: 

                2869    1725     732     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5623
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5623
 
BssSI C'ACGA_G

Cuts at:   2841    3683    4695    2841 
   Size:        842    1012    3769

  Fragments arranged by size: 

                3769    1012     842
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5623
 
BstBI TT'CG_AA

Cuts at:   4427    4427 
   Size:       5623
 
BstEII G'GTnAC_C

Cuts at:   1184    2855    1184 
   Size:       1671    3952
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2744    2867    2744 
   Size:        123    5500
 
BstYI r'GATC_y

Cuts at:   1972    2338    2395    2813    2953    3320    3705    3722    5174 
   Size:        366      57     418     140     367     385      17    1452

Cuts at:   5174    5260    5272    5618    1972 
   Size:         86      12     346    1977

  Fragments arranged by size: 

                1977    1452     418     385     367     366     346     140
                  86      57      17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2091    2646     513 
   Size:        552    1026     555    3490

  Fragments arranged by size: 

                3490    1026     555     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4884

  Fragments arranged by size: 

                4884     683      56
 
BtsI GCAGTG_nn'

Cuts at:   3139    3933    3953    3139 
   Size:        794      20    4809

  Fragments arranged by size: 

                4809     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5623
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5588
 
DraI TTT'AAA

Cuts at:   2440    2927    2440 
   Size:        487    5136
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4630     809 
   Size:        507    3314    1802

  Fragments arranged by size: 

                3314    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    3070    3959    5588 
   Size:         54    1425      50     275      18    1240     889    1629

Cuts at:   5588       8 
   Size:         43

  Fragments arranged by size: 

                1629    1425    1240     889     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3070    1537 
   Size:        275    1258    4090

  Fragments arranged by size: 

                4090    1258     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2583    2830    2842    3553    1353 
   Size:       1230     247      12     711    3423

  Fragments arranged by size: 

                3423    1230     711     247      12
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    2166    4210    4584    4730     387 
   Size:       1064     138     577    2044     374     146    1280

  Fragments arranged by size: 

                2044    1280    1064     577     374     146     138
 
EcoICRI GAG'CTC

Cuts at:   2641    2840    2641 
   Size:        199    5424
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2184    2286    2363    2408    2559    2607    3686 
   Size:        393     552     102      77      45     151      48    1079

Cuts at:   3686    4283    5070    5338    1239 
   Size:        597     787     268    1524

  Fragments arranged by size: 

                1524    1079     787     597     552     393     268     151
                 102      77      48      45
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2403    2403 
   Size:       5623
 
EcoRI G'AATT_C

Cuts at:   2932    3310    2932 
   Size:        378    5245
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2368    2368 
   Size:       5623
 
FspI TGC'GCA

Cuts at:   4129    4129 
   Size:       5623
 
HaeII r_GCGC'y

Cuts at:   1678    2187    4770    1678 
   Size:        509    2583    2531

  Fragments arranged by size: 

                2583    2531     509
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2615    2647    4264    4296    4338 
   Size:         32     135      32    1043      32    1617      32      42

Cuts at:   4338    4370    1373 
   Size:         32    2626

  Fragments arranged by size: 

                2626    1617    1043     135      42      32      32      32
                  32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2967    3213     669 
   Size:        988     281    1029     246    3079

  Fragments arranged by size: 

                3079    1029     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5623
 
HpaI GTT'AAC

Cuts at:   3213    3213 
   Size:       5623
 
KpnI G_GTAC'C

Cuts at:   2948    2948 
   Size:       5623
 
MfeI C'AATT_G

Cuts at:   3222    3222 
   Size:       5623
 
MluI A'CGCG_T

Cuts at:   5499    5499 
   Size:       5623
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    1989    2172    4737    4921    1305 
   Size:        684     183    2565     184    2007

  Fragments arranged by size: 

                2565    2007     684     184     183
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3801

  Fragments arranged by size: 

                3801    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3581    3940    4099     518 
   Size:        648    2415     359     159    2042

  Fragments arranged by size: 

                2415    2042     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2045    2117    2284    3039    3716    4864    5109    1606 
   Size:        439      72     167     755     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     755     677     439     245     167      72
            
 
NcoI C'CATG_G

Cuts at:    513    1065    2091    2646     513 
   Size:        552    1026     555    3490

  Fragments arranged by size: 

                3490    1026     555     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5623
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5623
 
NotI GC'GGCC_GC

Cuts at:   3070    3070 
   Size:       5623
 
NspI r_CATG'y

Cuts at:   1132    1229    2981    4526    1132 
   Size:         97    1752    1545    2229

  Fragments arranged by size: 

                2229    1752    1545      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5623
 
PciI A'CATG_T

Cuts at:   4522    4522 
   Size:       5623
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5623
 
PmeI GTTT'AAAC

Cuts at:   2927    2927 
   Size:       5623
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2003    2035    3692    3724    5205    5237    2003 
   Size:         32    1657      32    1481      32    2389

  Fragments arranged by size: 

                2389    1657    1481      32      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5623
 
PsiI TTA'TAA

Cuts at:   3193    3193 
   Size:       5623
 
PstI C_TGCA'G

Cuts at:    830    2975     830 
   Size:       2145    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5623
 
SacI G_AGCT'C

Cuts at:   2643    2842    2643 
   Size:        199    5424
 
SalI G'TCGA_C

Cuts at:   1936    2965    1936 
   Size:       1029    4594
 
SapI GCTCTTCn'nnn_

Cuts at:   2830    2830 
   Size:       5623
 
SbfI CC_TGCA'GG

Cuts at:   2975    2975 
   Size:       5623
 
ScaI AGT'ACT

Cuts at:   2606    3871    5583    2606 
   Size:       1265    1712    2646

  Fragments arranged by size: 

                2646    1712    1265
 
SfcI C'TryA_G

Cuts at:    826    1045    2971    4106    4787    4978     826 
   Size:        219    1926    1135     681     191    1471

  Fragments arranged by size: 

                1926    1471    1135     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5623
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5623
 
SmaI CCC'GGG

Cuts at:   1872    2950    1872 
   Size:       1078    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3727    4628    4890     820 
   Size:        197     926    1784     901     262    1553

  Fragments arranged by size: 

                1784    1553     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3414     493 
   Size:       2921    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5623
 
SphI G_CATG'C

Cuts at:   1132    2981    1132 
   Size:       1849    3774
 
SspI AAT'ATT

Cuts at:      5      52    3547       5 
   Size:         47    3495    2081

  Fragments arranged by size: 

                3495    2081      47
 
StuI AGG'CCT

Cuts at:   1517    2313    1517 
   Size:        796    4827
 
StyI C'CwwG_G

Cuts at:    513    1065    2091    2646    3062     513 
   Size:        552    1026     555     416    3074

  Fragments arranged by size: 

                3074    1026     555     552     416
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3650    3667    3820    4005    4450     325 
   Size:       1255    2070      17     153     185     445    1498

  Fragments arranged by size: 

                2070    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2604    3869    5581 
   Size:        274      80      33      51    1443     627    1265    1712

Cuts at:   5581      96 
   Size:        138

  Fragments arranged by size: 

                1712    1443    1265     627     274     138      80      51
                  33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2079    2111    5583    5615    2079 
   Size:         32    3472      32    2087

  Fragments arranged by size: 

                3472    2087      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5623
 
XbaI T'CTAG_A

Cuts at:   2816    2959    2816 
   Size:        143    5480
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5623
 
XmaI C'CCGG_G

Cuts at:   1870    2948    1870 
   Size:       1078    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3752    3752 
   Size:       5623
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3431     276 
   Size:         53      83     186     936    1897    2468

  Fragments arranged by size: 

                2468    1897     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI    EcoRI     FalI     FspI    HaeII
    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI
     MscI     MslI   MspA1I     NcoI     NdeI     NheI     NotI     NspI
     PasI     PciI    PflMI     PmeI     PpiI    PshAI     PsiI     PstI
     PvuI     SacI     SalI     SapI     SbfI     ScaI     SfcI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     ApaI     AscI    AvrII     BaeI     BbeI    BbvCI
    BfrBI     BplI    BsiWI    BsmBI  BstZ17I   Bsu36I   DraIII EcoO109I
    EcoRV     FseI    FspAI     KasI     NaeI     NarI   NgoMIV     NruI
     NsiI     PacI     PfoI     PmlI    PpuMI   PspOMI    PspXI     PsrI
    PvuII    RsrII    SacII    SanDI    SexAI     SfiI     SfoI     SrfI
     SwaI     XcmI
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