(Circular) (Six-base) MAPSORT of: pFN21AB8275.seq Check: 9912 from: 1 to: 5623 pFN21AB8275 5623 bp With 182 enzymes: SgfI * February 27, 2009 14:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3433 278 Size: 53 83 186 936 1897 2468 Fragments arranged by size: 2468 1897 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2376 2966 1937 Size: 439 590 4594 Fragments arranged by size: 4594 590 439 Acc65I G'GTAC_C Cuts at: 2944 2944 Size: 5623 AclI AA'CG_TT Cuts at: 1563 3750 4123 1563 Size: 2187 373 3063 Fragments arranged by size: 3063 2187 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2286 2363 2408 2607 3686 5070 5338 1632 Size: 654 77 45 199 1079 1384 268 1917 Fragments arranged by size: 1917 1384 1079 654 268 199 77 45 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5426 AflIII A'CryG_T Cuts at: 1203 4522 5499 1203 Size: 3319 977 1327 Fragments arranged by size: 3319 1327 977 AgeI A'CCGG_T Cuts at: 4454 4454 Size: 5623 AhdI GACnn_n'nnGTC Cuts at: 4352 4352 Size: 5623 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5623 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5623 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2003 2035 2003 Size: 32 5591 AlwNI CAG_nnn'CTG Cuts at: 3366 4938 3366 Size: 1572 4051 ApaLI G'TGCA_C Cuts at: 3680 4836 3680 Size: 1156 4467 ApoI r'AATT_y Cuts at: 1493 2360 2553 2932 3158 3310 5419 1493 Size: 867 193 379 226 152 2109 1697 Fragments arranged by size: 2109 1697 867 379 226 193 152 AseI AT'TA_AT Cuts at: 160 1032 4177 160 Size: 872 3145 1606 Fragments arranged by size: 3145 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5623 AvaI C'yCGr_G Cuts at: 1870 1943 2948 1870 Size: 73 1005 4545 Fragments arranged by size: 4545 1005 73 BamHI G'GATC_C Cuts at: 2953 3320 2953 Size: 367 5256 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2168 2944 4399 618 Size: 325 217 627 381 776 1455 1842 Fragments arranged by size: 1842 1455 776 627 381 325 217 BanII G_rGCy'C Cuts at: 1391 2643 2842 2942 1391 Size: 1252 199 100 4072 Fragments arranged by size: 4072 1252 199 100 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4652 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3814 3848 1903 Size: 34 1877 34 3678 Fragments arranged by size: 3678 1877 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2893 3515 4725 2893 Size: 622 1210 3791 Fragments arranged by size: 3791 1210 622 BclI T'GATC_A Cuts at: 1548 1548 Size: 5623 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2096 4234 136 Size: 107 122 71 631 605 424 2138 1525 Fragments arranged by size: 2138 1525 631 605 424 122 107 71 BglII A'GATC_T Cuts at: 2338 5618 2338 Size: 3280 2343 BlpI GC'TnA_GC Cuts at: 3040 3040 Size: 5623 Bme1580I G_kGCm'C Cuts at: 1790 3684 4840 1790 Size: 1894 1156 2573 Fragments arranged by size: 2573 1894 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5623 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3354 4312 442 Size: 416 53 83 399 24 1937 958 1753 Fragments arranged by size: 1937 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5623 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2184 2559 4283 1239 Size: 945 375 1724 2579 Fragments arranged by size: 2579 1724 945 375 Bpu10I CC'TnA_GC Cuts at: 2315 2315 Size: 5623 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3748 4613 4911 3748 Size: 865 298 4460 Fragments arranged by size: 4460 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4286 882 Size: 617 2787 2219 Fragments arranged by size: 2787 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3414 493 Size: 711 2210 2702 Fragments arranged by size: 2702 2210 711 BsaBI GATnn'nnATC Cuts at: 5617 5617 Size: 5623 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2211 3430 3812 4447 Size: 53 83 186 936 678 1219 382 635 Cuts at: 4447 275 Size: 1451 Fragments arranged by size: 1451 1219 936 678 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4056 4454 4728 4875 5286 4056 Size: 398 274 147 411 4393 Fragments arranged by size: 4393 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2003 2033 2079 2109 1178 Size: 30 165 30 600 30 46 30 4692 Fragments arranged by size: 4692 600 165 46 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2027 2239 2240 2835 1186 Size: 841 212 1 595 3974 Fragments arranged by size: 3974 841 595 212 1 BseYI C'CCAG_C Cuts at: 2319 4826 5322 2319 Size: 2507 496 2620 Fragments arranged by size: 2620 2507 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2365 1163 Size: 573 629 4421 Fragments arranged by size: 4421 629 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3073 3834 4862 1540 Size: 275 186 1072 761 1028 2301 Fragments arranged by size: 2301 1072 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2643 2842 3684 3769 4840 2643 Size: 199 842 85 1071 3426 Fragments arranged by size: 3426 1071 842 199 85 BsmI GAATG_Cn' Cuts at: 3134 3227 3134 Size: 93 5530 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2643 2842 2942 3684 3769 4840 1391 Size: 399 853 199 100 742 85 1071 2174 Fragments arranged by size: 2174 1071 853 742 399 199 100 85 BspEI T'CCGG_A Cuts at: 5286 5286 Size: 5623 BspHI T'CATG_A Cuts at: 3512 5242 3512 Size: 1730 3893 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2978 844 Size: 2134 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3510 1394 Size: 429 1687 3507 Fragments arranged by size: 3507 1687 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4118 4292 65 Size: 1183 2870 174 1396 Fragments arranged by size: 2870 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2542 4267 4454 1700 Size: 110 732 1725 187 2869 Fragments arranged by size: 2869 1725 732 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5623 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5623 BssSI C'ACGA_G Cuts at: 2841 3683 4695 2841 Size: 842 1012 3769 Fragments arranged by size: 3769 1012 842 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5623 BstBI TT'CG_AA Cuts at: 4427 4427 Size: 5623 BstEII G'GTnAC_C Cuts at: 1184 2855 1184 Size: 1671 3952 BstXI CCAn_nnnn'nTGG Cuts at: 2744 2867 2744 Size: 123 5500 BstYI r'GATC_y Cuts at: 1972 2338 2395 2813 2953 3320 3705 3722 5174 Size: 366 57 418 140 367 385 17 1452 Cuts at: 5174 5260 5272 5618 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 418 385 367 366 346 140 86 57 17 12 BtgI C'CryG_G Cuts at: 513 1065 2091 2646 513 Size: 552 1026 555 3490 Fragments arranged by size: 3490 1026 555 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4884 Fragments arranged by size: 4884 683 56 BtsI GCAGTG_nn' Cuts at: 3139 3933 3953 3139 Size: 794 20 4809 Fragments arranged by size: 4809 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5623 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5588 DraI TTT'AAA Cuts at: 2440 2927 2440 Size: 487 5136 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4630 809 Size: 507 3314 1802 Fragments arranged by size: 3314 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3070 3959 5588 Size: 54 1425 50 275 18 1240 889 1629 Cuts at: 5588 8 Size: 43 Fragments arranged by size: 1629 1425 1240 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3070 1537 Size: 275 1258 4090 Fragments arranged by size: 4090 1258 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2583 2830 2842 3553 1353 Size: 1230 247 12 711 3423 Fragments arranged by size: 3423 1230 711 247 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2166 4210 4584 4730 387 Size: 1064 138 577 2044 374 146 1280 Fragments arranged by size: 2044 1280 1064 577 374 146 138 EcoICRI GAG'CTC Cuts at: 2641 2840 2641 Size: 199 5424 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2184 2286 2363 2408 2559 2607 3686 Size: 393 552 102 77 45 151 48 1079 Cuts at: 3686 4283 5070 5338 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1079 787 597 552 393 268 151 102 77 48 45 EcoNI CCTnn'n_nnAGG Cuts at: 2403 2403 Size: 5623 EcoRI G'AATT_C Cuts at: 2932 3310 2932 Size: 378 5245 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2368 2368 Size: 5623 FspI TGC'GCA Cuts at: 4129 4129 Size: 5623 HaeII r_GCGC'y Cuts at: 1678 2187 4770 1678 Size: 509 2583 2531 Fragments arranged by size: 2583 2531 509 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2615 2647 4264 4296 4338 Size: 32 135 32 1043 32 1617 32 42 Cuts at: 4338 4370 1373 Size: 32 2626 Fragments arranged by size: 2626 1617 1043 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2967 3213 669 Size: 988 281 1029 246 3079 Fragments arranged by size: 3079 1029 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5623 HpaI GTT'AAC Cuts at: 3213 3213 Size: 5623 KpnI G_GTAC'C Cuts at: 2948 2948 Size: 5623 MfeI C'AATT_G Cuts at: 3222 3222 Size: 5623 MluI A'CGCG_T Cuts at: 5499 5499 Size: 5623 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 1989 2172 4737 4921 1305 Size: 684 183 2565 184 2007 Fragments arranged by size: 2565 2007 684 184 183 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3801 Fragments arranged by size: 3801 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3581 3940 4099 518 Size: 648 2415 359 159 2042 Fragments arranged by size: 2415 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2045 2117 2284 3039 3716 4864 5109 1606 Size: 439 72 167 755 677 1148 245 2120 Fragments arranged by size: 2120 1148 755 677 439 245 167 72 NcoI C'CATG_G Cuts at: 513 1065 2091 2646 513 Size: 552 1026 555 3490 Fragments arranged by size: 3490 1026 555 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5623 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5623 NotI GC'GGCC_GC Cuts at: 3070 3070 Size: 5623 NspI r_CATG'y Cuts at: 1132 1229 2981 4526 1132 Size: 97 1752 1545 2229 Fragments arranged by size: 2229 1752 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5623 PciI A'CATG_T Cuts at: 4522 4522 Size: 5623 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5623 PmeI GTTT'AAAC Cuts at: 2927 2927 Size: 5623 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2003 2035 3692 3724 5205 5237 2003 Size: 32 1657 32 1481 32 2389 Fragments arranged by size: 2389 1657 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5623 PsiI TTA'TAA Cuts at: 3193 3193 Size: 5623 PstI C_TGCA'G Cuts at: 830 2975 830 Size: 2145 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5623 SacI G_AGCT'C Cuts at: 2643 2842 2643 Size: 199 5424 SalI G'TCGA_C Cuts at: 1936 2965 1936 Size: 1029 4594 SapI GCTCTTCn'nnn_ Cuts at: 2830 2830 Size: 5623 SbfI CC_TGCA'GG Cuts at: 2975 2975 Size: 5623 ScaI AGT'ACT Cuts at: 2606 3871 5583 2606 Size: 1265 1712 2646 Fragments arranged by size: 2646 1712 1265 SfcI C'TryA_G Cuts at: 826 1045 2971 4106 4787 4978 826 Size: 219 1926 1135 681 191 1471 Fragments arranged by size: 1926 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5623 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5623 SmaI CCC'GGG Cuts at: 1872 2950 1872 Size: 1078 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3727 4628 4890 820 Size: 197 926 1784 901 262 1553 Fragments arranged by size: 1784 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3414 493 Size: 2921 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5623 SphI G_CATG'C Cuts at: 1132 2981 1132 Size: 1849 3774 SspI AAT'ATT Cuts at: 5 52 3547 5 Size: 47 3495 2081 Fragments arranged by size: 3495 2081 47 StuI AGG'CCT Cuts at: 1517 2313 1517 Size: 796 4827 StyI C'CwwG_G Cuts at: 513 1065 2091 2646 3062 513 Size: 552 1026 555 416 3074 Fragments arranged by size: 3074 1026 555 552 416 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3650 3667 3820 4005 4450 325 Size: 1255 2070 17 153 185 445 1498 Fragments arranged by size: 2070 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2604 3869 5581 Size: 274 80 33 51 1443 627 1265 1712 Cuts at: 5581 96 Size: 138 Fragments arranged by size: 1712 1443 1265 627 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2079 2111 5583 5615 2079 Size: 32 3472 32 2087 Fragments arranged by size: 3472 2087 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5623 XbaI T'CTAG_A Cuts at: 2816 2959 2816 Size: 143 5480 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5623 XmaI C'CCGG_G Cuts at: 1870 2948 1870 Size: 1078 4545 XmnI GAAnn'nnTTC Cuts at: 3752 3752 Size: 5623 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3431 276 Size: 53 83 186 936 1897 2468 Fragments arranged by size: 2468 1897 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI