(Circular) (Six-base) MAPSORT of: pFN21AB8271.seq Check: 3074 from: 1 to: 5656 pFN21AB8271 5656 bp With 182 enzymes: SgfI * February 27, 2009 14:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2185 2487 2752 2185 Size: 302 265 5089 Fragments arranged by size: 5089 302 265 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3466 278 Size: 53 83 186 936 1930 2468 Fragments arranged by size: 2468 1930 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2999 1937 Size: 1062 4594 Acc65I G'GTAC_C Cuts at: 2977 2977 Size: 5656 AclI AA'CG_TT Cuts at: 1563 3783 4156 1563 Size: 2220 373 3063 Fragments arranged by size: 3063 2220 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3719 5103 5371 1632 Size: 2087 1384 268 1917 Fragments arranged by size: 2087 1917 1384 268 AfeI AGC'GCT Cuts at: 2132 2132 Size: 5656 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5459 AflIII A'CryG_T Cuts at: 1203 2388 4555 5532 1203 Size: 1185 2167 977 1327 Fragments arranged by size: 2167 1327 1185 977 AgeI A'CCGG_T Cuts at: 4487 4487 Size: 5656 AhdI GACnn_n'nnGTC Cuts at: 4385 4385 Size: 5656 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5656 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5656 AlwNI CAG_nnn'CTG Cuts at: 2185 2236 3399 4971 2185 Size: 51 1163 1572 2870 Fragments arranged by size: 2870 1572 1163 51 ApaI G_GGCC'C Cuts at: 2557 2607 2680 2557 Size: 50 73 5533 Fragments arranged by size: 5533 73 50 ApaLI G'TGCA_C Cuts at: 3713 4869 3713 Size: 1156 4500 ApoI r'AATT_y Cuts at: 1493 2965 3191 3343 5452 1493 Size: 1472 226 152 2109 1697 Fragments arranged by size: 2109 1697 1472 226 152 AseI AT'TA_AT Cuts at: 160 1032 4210 160 Size: 872 3178 1606 Fragments arranged by size: 3178 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5656 AvaI C'yCGr_G Cuts at: 1870 1943 2093 2981 1870 Size: 73 150 888 4545 Fragments arranged by size: 4545 888 150 73 BamHI G'GATC_C Cuts at: 2986 3353 2986 Size: 367 5289 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2564 2921 2977 4432 618 Size: 325 217 627 777 357 56 1455 1842 Fragments arranged by size: 1842 1455 777 627 357 325 217 56 BanII G_rGCy'C Cuts at: 1391 2094 2557 2607 2648 2680 2859 2975 1391 Size: 703 463 50 41 32 179 116 4072 Fragments arranged by size: 4072 703 463 179 116 50 41 32 BbeI G_GCGC'C Cuts at: 2568 2568 Size: 5656 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2874 928 Size: 971 975 3710 Fragments arranged by size: 3710 975 971 BbvCI CC'TCA_GC Cuts at: 2579 2579 Size: 5656 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3847 3881 1903 Size: 34 1910 34 3678 Fragments arranged by size: 3678 1910 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3548 4758 3548 Size: 1210 4446 BclI T'GATC_A Cuts at: 1548 1548 Size: 5656 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2831 4267 136 Size: 107 122 71 631 605 1159 1436 1525 Fragments arranged by size: 1525 1436 1159 631 605 122 107 71 BglII A'GATC_T Cuts at: 5651 5651 Size: 5656 BlpI GC'TnA_GC Cuts at: 2213 3073 2213 Size: 860 4796 Bme1580I G_kGCm'C Cuts at: 1790 2332 2557 2607 2680 2924 2943 3717 4873 Size: 542 225 50 73 244 19 774 1156 Cuts at: 4873 1790 Size: 2573 Fragments arranged by size: 2573 1156 774 542 244 225 73 50 19 BmgBI CAC'GTC Cuts at: 1318 2389 1318 Size: 1071 4585 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2156 3387 4345 Size: 416 53 83 399 24 739 1231 958 Cuts at: 4345 442 Size: 1753 Fragments arranged by size: 1753 1231 958 739 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5656 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2312 2807 4316 1239 Size: 1073 495 1509 2579 Fragments arranged by size: 2579 1509 1073 495 Bpu10I CC'TnA_GC Cuts at: 2579 2579 Size: 5656 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2825 3781 4646 4944 2825 Size: 956 865 298 3537 Fragments arranged by size: 3537 956 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2249 4319 882 Size: 617 750 2070 2219 Fragments arranged by size: 2219 2070 750 617 BsaAI yAC'GTr Cuts at: 493 1204 3447 493 Size: 711 2243 2702 Fragments arranged by size: 2702 2243 711 BsaBI GATnn'nnATC Cuts at: 5650 5650 Size: 5656 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2565 3463 3845 4480 Size: 53 83 186 936 1032 898 382 635 Cuts at: 4480 275 Size: 1451 Fragments arranged by size: 1451 1032 936 898 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4089 4487 4761 4908 5319 4089 Size: 398 274 147 411 4426 Fragments arranged by size: 4426 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2375 2405 1178 Size: 30 165 30 972 30 4429 Fragments arranged by size: 4429 972 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2219 2333 2465 2492 2729 2804 1186 Size: 1033 114 132 27 237 75 4038 Fragments arranged by size: 4038 1033 237 132 114 75 27 BseYI C'CCAG_C Cuts at: 2133 2172 2368 2799 2942 4859 5355 2133 Size: 39 196 431 143 1917 496 2434 Fragments arranged by size: 2434 1917 496 431 196 143 39 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2198 2219 2426 2444 2927 1163 Size: 573 462 21 207 18 483 3892 Fragments arranged by size: 3892 573 483 462 207 21 18 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2398 2740 3106 3867 4895 1540 Size: 275 186 397 342 366 761 1028 2301 Fragments arranged by size: 2301 1028 761 397 366 342 275 186 BsiHKAI G_wGCw'C Cuts at: 2447 3717 3802 4873 2447 Size: 1270 85 1071 3230 Fragments arranged by size: 3230 1270 1071 85 BsmI GAATG_Cn' Cuts at: 3167 3260 3167 Size: 93 5563 BsmBI CGTCTCn'nnnn_ Cuts at: 2315 2315 Size: 5656 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2094 2332 2447 2557 2607 2648 2680 Size: 399 304 238 115 110 50 41 32 Cuts at: 2680 2859 2924 2943 2975 3717 3802 4873 1391 Size: 179 65 19 32 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 304 238 179 115 110 85 65 50 41 32 32 19 BspEI T'CCGG_A Cuts at: 5319 5319 Size: 5656 BspHI T'CATG_A Cuts at: 3545 5275 3545 Size: 1730 3926 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2175 2185 2487 2529 2752 3011 844 Size: 1331 10 302 42 223 259 3489 Fragments arranged by size: 3489 1331 302 259 223 42 10 BsrBI CCG'CTC Cuts at: 1394 1823 2124 2674 2695 3543 1394 Size: 429 301 550 21 848 3507 Fragments arranged by size: 3507 848 550 429 301 21 BsrDI GCAATG_nn' Cuts at: 65 1248 4151 4325 65 Size: 1183 2903 174 1396 Fragments arranged by size: 2903 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2654 4300 4487 1700 Size: 110 844 1646 187 2869 Fragments arranged by size: 2869 1646 844 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5656 BssHII G'CGCG_C Cuts at: 1925 2701 2703 2776 1925 Size: 776 2 73 4805 Fragments arranged by size: 4805 776 73 2 BssSI C'ACGA_G Cuts at: 3716 4728 3716 Size: 1012 4644 BstAPI GCAn_nnn'nTGC Cuts at: 794 2431 794 Size: 1637 4019 BstBI TT'CG_AA Cuts at: 4460 4460 Size: 5656 BstEII G'GTnAC_C Cuts at: 1184 2224 1184 Size: 1040 4616 BstYI r'GATC_y Cuts at: 1972 2986 3353 3738 3755 5207 5293 5305 5651 Size: 1014 367 385 17 1452 86 12 346 Cuts at: 5651 1972 Size: 1977 Fragments arranged by size: 1977 1452 1014 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2413 2916 513 Size: 552 1348 503 3253 Fragments arranged by size: 3253 1348 552 503 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2523 487 Size: 683 56 1297 3620 Fragments arranged by size: 3620 1297 683 56 BtsI GCAGTG_nn' Cuts at: 2544 3172 3966 3986 2544 Size: 628 794 20 4214 Fragments arranged by size: 4214 794 628 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5656 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5621 DraI TTT'AAA Cuts at: 2960 2960 Size: 5656 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4663 809 Size: 507 3347 1802 Fragments arranged by size: 3347 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2125 2395 2437 Size: 54 1425 50 275 18 295 270 42 Cuts at: 2437 2652 2656 3103 3992 5621 8 Size: 215 4 447 889 1629 43 Fragments arranged by size: 1629 1425 889 447 295 275 270 215 54 50 43 42 18 4 EagI C'GGCC_G Cuts at: 1537 1812 2395 3103 1537 Size: 275 583 708 4090 Fragments arranged by size: 4090 708 583 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3586 1353 Size: 2233 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2330 2783 4243 4617 4763 387 Size: 1064 138 741 453 1460 374 146 1280 Fragments arranged by size: 1460 1280 1064 741 453 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2312 2807 3719 4316 5103 5371 1239 Size: 393 680 495 912 597 787 268 1524 Fragments arranged by size: 1524 912 787 680 597 495 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2747 2747 Size: 5656 EcoO109I rG'GnC_Cy Cuts at: 2553 2603 2604 2553 Size: 50 1 5605 Fragments arranged by size: 5605 50 1 EcoRI G'AATT_C Cuts at: 2965 3343 2965 Size: 378 5278 FseI GG_CCGG'CC Cuts at: 2658 2658 Size: 5656 FspI TGC'GCA Cuts at: 2483 4162 2483 Size: 1679 3977 HaeII r_GCGC'y Cuts at: 1678 2134 2246 2533 2568 2586 2599 2881 2931 Size: 456 112 287 35 18 13 282 50 Cuts at: 2931 4803 1678 Size: 1872 2531 Fragments arranged by size: 2531 1872 456 287 282 112 50 35 18 13 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4297 4329 4371 4403 1373 Size: 32 135 32 2725 32 42 32 2626 Fragments arranged by size: 2725 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3000 3246 669 Size: 988 281 1062 246 3079 Fragments arranged by size: 3079 1062 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5656 HpaI GTT'AAC Cuts at: 3246 3246 Size: 5656 KasI G'GCGC_C Cuts at: 2564 2564 Size: 5656 KpnI G_GTAC'C Cuts at: 2981 2981 Size: 5656 MfeI C'AATT_G Cuts at: 3255 3255 Size: 5656 MluI A'CGCG_T Cuts at: 5532 5532 Size: 5656 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4770 4954 1305 Size: 3465 184 2007 Fragments arranged by size: 3465 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3834 Fragments arranged by size: 3834 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3614 3973 4132 518 Size: 648 2448 359 159 2042 Fragments arranged by size: 2448 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2415 3072 3749 4897 5142 1606 Size: 809 657 677 1148 245 2120 Fragments arranged by size: 2120 1148 809 677 657 245 NaeI GCC'GGC Cuts at: 2656 2656 Size: 5656 NarI GG'CG_CC Cuts at: 2565 2565 Size: 5656 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5104 NdeI CA'TA_TG Cuts at: 387 387 Size: 5656 NgoMIV G'CCGG_C Cuts at: 2654 2654 Size: 5656 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5656 NotI GC'GGCC_GC Cuts at: 2395 3103 2395 Size: 708 4948 NspI r_CATG'y Cuts at: 1132 1229 2875 3014 4559 1132 Size: 97 1646 139 1545 2229 Fragments arranged by size: 2229 1646 1545 139 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5656 PciI A'CATG_T Cuts at: 4555 4555 Size: 5656 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5656 PmeI GTTT'AAAC Cuts at: 2960 2960 Size: 5656 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3725 3757 5238 5270 3725 Size: 32 1481 32 4111 Fragments arranged by size: 4111 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5656 PsiI TTA'TAA Cuts at: 3226 3226 Size: 5656 PspOMI G'GGCC_C Cuts at: 2553 2603 2676 2553 Size: 50 73 5533 Fragments arranged by size: 5533 73 50 PstI C_TGCA'G Cuts at: 830 2352 2526 2817 3008 830 Size: 1522 174 291 191 3478 Fragments arranged by size: 3478 1522 291 191 174 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5656 SacII CC_GC'GG Cuts at: 2416 2416 Size: 5656 SalI G'TCGA_C Cuts at: 1936 2998 1936 Size: 1062 4594 SbfI CC_TGCA'GG Cuts at: 2817 3008 2817 Size: 191 5465 ScaI AGT'ACT Cuts at: 3904 5616 3904 Size: 1712 3944 SfcI C'TryA_G Cuts at: 826 1045 2348 2522 2666 2813 3004 4139 4820 Size: 219 1303 174 144 147 191 1135 681 Cuts at: 4820 5011 826 Size: 191 1471 Fragments arranged by size: 1471 1303 1135 681 219 191 191 174 147 144 SfiI GGCCn_nnn'nGGCC Cuts at: 2831 2831 Size: 5656 SfoI GGC'GCC Cuts at: 2566 2566 Size: 5656 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5656 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5656 SmaI CCC'GGG Cuts at: 1872 2983 1872 Size: 1111 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2840 3760 4661 4923 820 Size: 197 926 897 920 901 262 1553 Fragments arranged by size: 1553 926 920 901 897 262 197 SnaBI TAC'GTA Cuts at: 493 3447 493 Size: 2954 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5656 SphI G_CATG'C Cuts at: 1132 3014 1132 Size: 1882 3774 SspI AAT'ATT Cuts at: 5 52 3580 5 Size: 47 3528 2081 Fragments arranged by size: 3528 2081 47 StuI AGG'CCT Cuts at: 1517 2276 2684 2825 1517 Size: 759 408 141 4348 Fragments arranged by size: 4348 759 408 141 StyI C'CwwG_G Cuts at: 513 1065 2058 2340 3095 513 Size: 552 993 282 755 3074 Fragments arranged by size: 3074 993 755 552 282 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2126 2164 2243 3683 3700 3853 4038 Size: 1255 546 38 79 1440 17 153 185 Cuts at: 4038 4483 325 Size: 445 1498 Fragments arranged by size: 1498 1440 1255 546 445 185 153 79 38 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3902 5614 96 Size: 274 80 33 51 1443 1925 1712 138 Fragments arranged by size: 1925 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2375 2407 5616 5648 2375 Size: 32 3209 32 2383 Fragments arranged by size: 3209 2383 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2185 1316 Size: 869 4787 XbaI T'CTAG_A Cuts at: 2992 2992 Size: 5656 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5656 XmaI C'CCGG_G Cuts at: 1870 2981 1870 Size: 1111 4545 XmnI GAAnn'nnTTC Cuts at: 3785 3785 Size: 5656 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3464 276 Size: 53 83 186 936 1930 2468 Fragments arranged by size: 2468 1930 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI SrfI SwaI XcmI