(Circular) (Six-base) MAPSORT of: pFN21AB8267.seq Check: 4529 from: 1 to: 5398 pFN21AB8267 5398 bp With 182 enzymes: SgfI * February 27, 2009 14:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3208 278 Size: 53 83 186 936 1672 2468 Fragments arranged by size: 2468 1672 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2741 1937 Size: 804 4594 Acc65I G'GTAC_C Cuts at: 2719 2719 Size: 5398 AclI AA'CG_TT Cuts at: 1563 3525 3898 1563 Size: 1962 373 3063 Fragments arranged by size: 3063 1962 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3461 4845 5113 1632 Size: 1829 1384 268 1917 Fragments arranged by size: 1917 1829 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5201 AflIII A'CryG_T Cuts at: 1203 4297 5274 1203 Size: 3094 977 1327 Fragments arranged by size: 3094 1327 977 AgeI A'CCGG_T Cuts at: 4229 4229 Size: 5398 AhdI GACnn_n'nnGTC Cuts at: 4127 4127 Size: 5398 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5398 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2045 2048 1184 Size: 861 3 4534 Fragments arranged by size: 4534 861 3 AlwNI CAG_nnn'CTG Cuts at: 2462 2471 3141 4713 2462 Size: 9 670 1572 3147 Fragments arranged by size: 3147 1572 670 9 ApaI G_GGCC'C Cuts at: 2315 2576 2315 Size: 261 5137 ApaLI G'TGCA_C Cuts at: 3455 4611 3455 Size: 1156 4242 ApoI r'AATT_y Cuts at: 1493 2119 2707 2933 3085 5194 1493 Size: 626 588 226 152 2109 1697 Fragments arranged by size: 2109 1697 626 588 226 152 AseI AT'TA_AT Cuts at: 160 1032 3952 160 Size: 872 2920 1606 Fragments arranged by size: 2920 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5398 AvaI C'yCGr_G Cuts at: 1870 1943 2283 2723 1870 Size: 73 340 440 4545 Fragments arranged by size: 4545 440 340 73 BamHI G'GATC_C Cuts at: 2616 2728 3095 2616 Size: 112 367 4919 Fragments arranged by size: 4919 367 112 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2090 2719 4174 618 Size: 325 217 627 303 629 1455 1842 Fragments arranged by size: 1842 1455 629 627 325 303 217 BanII G_rGCy'C Cuts at: 1391 2315 2497 2576 2717 1391 Size: 924 182 79 141 4072 Fragments arranged by size: 4072 924 182 141 79 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4427 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3589 3623 1903 Size: 34 1652 34 3678 Fragments arranged by size: 3678 1652 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3290 4500 3290 Size: 1210 4188 BclI T'GATC_A Cuts at: 1548 1548 Size: 5398 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4009 136 Size: 107 122 71 631 605 2337 1525 Fragments arranged by size: 2337 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5393 5393 Size: 5398 BlpI GC'TnA_GC Cuts at: 2815 2815 Size: 5398 Bme1580I G_kGCm'C Cuts at: 1790 2315 2576 3459 4615 1790 Size: 525 261 883 1156 2573 Fragments arranged by size: 2573 1156 883 525 261 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5398 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2497 3129 4087 Size: 416 53 83 399 24 1080 632 958 Cuts at: 4087 442 Size: 1753 Fragments arranged by size: 1753 1080 958 632 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5398 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2319 2544 4058 1239 Size: 1080 225 1514 2579 Fragments arranged by size: 2579 1514 1080 225 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3523 4388 4686 3523 Size: 865 298 4235 Fragments arranged by size: 4235 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2242 2584 4061 882 Size: 617 743 342 1477 2219 Fragments arranged by size: 2219 1477 743 617 342 BsaAI yAC'GTr Cuts at: 493 1204 3189 493 Size: 711 1985 2702 Fragments arranged by size: 2702 1985 711 BsaBI GATnn'nnATC Cuts at: 5392 5392 Size: 5398 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3205 3587 4222 275 Size: 53 83 186 936 1672 382 635 1451 Fragments arranged by size: 1672 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3831 4229 4503 4650 5061 3831 Size: 398 274 147 411 4168 Fragments arranged by size: 4168 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5173 Fragments arranged by size: 5173 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2250 2562 2661 1186 Size: 1064 312 99 3923 Fragments arranged by size: 3923 1064 312 99 BseYI C'CCAG_C Cuts at: 2347 2351 2387 4601 5097 2347 Size: 4 36 2214 496 2648 Fragments arranged by size: 2648 2214 496 36 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4825 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2077 2848 3609 4637 1540 Size: 275 186 76 771 761 1028 2301 Fragments arranged by size: 2301 1028 771 761 275 186 76 BsiHKAI G_wGCw'C Cuts at: 2554 3459 3544 4615 2554 Size: 905 85 1071 3337 Fragments arranged by size: 3337 1071 905 85 BsmI GAATG_Cn' Cuts at: 2667 2909 3002 2667 Size: 242 93 5063 Fragments arranged by size: 5063 242 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2315 2497 2554 2576 2717 3459 3544 Size: 399 525 182 57 22 141 742 85 Cuts at: 3544 4615 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 525 399 182 141 85 57 22 BspEI T'CCGG_A Cuts at: 5061 5061 Size: 5398 BspHI T'CATG_A Cuts at: 3287 5017 3287 Size: 1730 3668 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2753 844 Size: 1909 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3285 1394 Size: 429 1462 3507 Fragments arranged by size: 3507 1462 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3893 4067 65 Size: 1183 2645 174 1396 Fragments arranged by size: 2645 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4042 4229 1700 Size: 110 2232 187 2869 Fragments arranged by size: 2869 2232 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5398 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5398 BssSI C'ACGA_G Cuts at: 3458 4470 3458 Size: 1012 4386 BstAPI GCAn_nnn'nTGC Cuts at: 794 2384 2462 2471 794 Size: 1590 78 9 3721 Fragments arranged by size: 3721 1590 78 9 BstBI TT'CG_AA Cuts at: 4202 4202 Size: 5398 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5398 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 5398 BstYI r'GATC_y Cuts at: 1972 2079 2616 2728 3095 3480 3497 4949 5035 Size: 107 537 112 367 385 17 1452 86 Cuts at: 5035 5047 5393 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 537 385 367 346 112 107 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2398 2518 513 Size: 552 1333 120 3393 Fragments arranged by size: 3393 1333 552 120 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4659 Fragments arranged by size: 4659 683 56 BtsI GCAGTG_nn' Cuts at: 2914 3708 3728 2914 Size: 794 20 4584 Fragments arranged by size: 4584 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5398 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5363 DraI TTT'AAA Cuts at: 2702 2702 Size: 5398 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4405 809 Size: 507 3089 1802 Fragments arranged by size: 3089 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2074 2515 2845 Size: 54 1425 50 275 18 244 441 330 Cuts at: 2845 3734 5363 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 441 330 275 244 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2074 2845 1537 Size: 275 262 771 4090 Fragments arranged by size: 4090 771 275 262 EarI CTCTTCn'nnn_ Cuts at: 1353 2184 2437 2623 3328 1353 Size: 831 253 186 705 3423 Fragments arranged by size: 3423 831 705 253 186 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3985 4359 4505 387 Size: 1064 138 2396 374 146 1280 Fragments arranged by size: 2396 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2319 2544 3461 4058 4845 5113 1239 Size: 393 687 225 917 597 787 268 1524 Fragments arranged by size: 1524 917 787 687 597 393 268 225 EcoNI CCTnn'n_nnAGG Cuts at: 2628 2628 Size: 5398 EcoO109I rG'GnC_Cy Cuts at: 2255 2311 2525 2603 2255 Size: 56 214 78 5050 Fragments arranged by size: 5050 214 78 56 EcoRI G'AATT_C Cuts at: 2707 3085 2707 Size: 378 5020 FspI TGC'GCA Cuts at: 3904 3904 Size: 5398 HaeII r_GCGC'y Cuts at: 1678 4545 1678 Size: 2867 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4039 4071 4113 4145 1373 Size: 32 135 32 2467 32 42 32 2626 Fragments arranged by size: 2626 2467 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2742 2988 669 Size: 988 281 804 246 3079 Fragments arranged by size: 3079 988 804 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5398 HpaI GTT'AAC Cuts at: 2988 2988 Size: 5398 KpnI G_GTAC'C Cuts at: 2723 2723 Size: 5398 MfeI C'AATT_G Cuts at: 2997 2997 Size: 5398 MluI A'CGCG_T Cuts at: 5274 5274 Size: 5398 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2522 4512 4696 1305 Size: 1217 1990 184 2007 Fragments arranged by size: 2007 1990 1217 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3576 Fragments arranged by size: 3576 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 3356 3715 3874 518 Size: 648 843 1347 359 159 2042 Fragments arranged by size: 2042 1347 843 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2351 2462 2468 2814 3491 4639 4884 1606 Size: 745 111 6 346 677 1148 245 2120 Fragments arranged by size: 2120 1148 745 677 346 245 111 6 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4846 NdeI CA'TA_TG Cuts at: 387 387 Size: 5398 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5398 NotI GC'GGCC_GC Cuts at: 2845 2845 Size: 5398 NspI r_CATG'y Cuts at: 1132 1229 2650 2756 4301 1132 Size: 97 1421 106 1545 2229 Fragments arranged by size: 2229 1545 1421 106 97 PasI CC'CwG_GG Cuts at: 1348 2315 2678 1348 Size: 967 363 4068 Fragments arranged by size: 4068 967 363 PciI A'CATG_T Cuts at: 4297 4297 Size: 5398 PflMI CCAn_nnn'nTGG Cuts at: 1108 2297 2399 2582 1108 Size: 1189 102 183 3924 Fragments arranged by size: 3924 1189 183 102 PfoI T'CCnGG_A Cuts at: 2656 2656 Size: 5398 PmeI GTTT'AAAC Cuts at: 2702 2702 Size: 5398 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3467 3499 4980 5012 3467 Size: 32 1481 32 3853 Fragments arranged by size: 3853 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2255 2525 2603 2255 Size: 270 78 5050 Fragments arranged by size: 5050 270 78 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5398 PsiI TTA'TAA Cuts at: 2968 2968 Size: 5398 PspOMI G'GGCC_C Cuts at: 2311 2572 2311 Size: 261 5137 PspXI vC'TCGA_Gb Cuts at: 2283 2283 Size: 5398 PstI C_TGCA'G Cuts at: 830 2037 2070 2380 2467 2750 830 Size: 1207 33 310 87 283 3478 Fragments arranged by size: 3478 1207 310 283 87 33 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5398 PvuII CAG'CTG Cuts at: 2351 2468 2351 Size: 117 5281 SalI G'TCGA_C Cuts at: 1936 2740 1936 Size: 804 4594 SanDI GG'GwC_CC Cuts at: 2603 2603 Size: 5398 SbfI CC_TGCA'GG Cuts at: 2070 2380 2750 2070 Size: 310 370 4718 Fragments arranged by size: 4718 370 310 ScaI AGT'ACT Cuts at: 3646 5358 3646 Size: 1712 3686 SfcI C'TryA_G Cuts at: 826 1045 2033 2066 2376 2463 2746 3881 4562 Size: 219 988 33 310 87 283 1135 681 Cuts at: 4562 4753 826 Size: 191 1471 Fragments arranged by size: 1471 1135 988 681 310 283 219 191 87 33 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5398 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5398 SmaI CCC'GGG Cuts at: 1872 2725 1872 Size: 853 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2283 3502 4403 4665 820 Size: 197 926 340 1219 901 262 1553 Fragments arranged by size: 1553 1219 926 901 340 262 197 SnaBI TAC'GTA Cuts at: 493 3189 493 Size: 2696 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5398 SphI G_CATG'C Cuts at: 1132 2650 2756 1132 Size: 1518 106 3774 Fragments arranged by size: 3774 1518 106 SspI AAT'ATT Cuts at: 5 52 3322 5 Size: 47 3270 2081 Fragments arranged by size: 3270 2081 47 StuI AGG'CCT Cuts at: 1517 2691 1517 Size: 1174 4224 StyI C'CwwG_G Cuts at: 513 1065 2837 513 Size: 552 1772 3074 Fragments arranged by size: 3074 1772 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2195 3425 3442 3595 3780 4225 325 Size: 1255 615 1230 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1230 615 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3644 5356 96 Size: 274 80 33 51 1443 1667 1712 138 Fragments arranged by size: 1712 1667 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5358 5390 5358 Size: 32 5366 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5398 XbaI T'CTAG_A Cuts at: 2734 2734 Size: 5398 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2510 2510 Size: 5398 XhoI C'TCGA_G Cuts at: 1943 2283 1943 Size: 340 5058 XmaI C'CCGG_G Cuts at: 1870 2723 1870 Size: 853 4545 XmnI GAAnn'nnTTC Cuts at: 3527 3527 Size: 5398 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3206 276 Size: 53 83 186 936 1672 2468 Fragments arranged by size: 2468 1672 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI