(Circular) (Six-base) MAPSORT of: pFN21AB8263.seq Check: 3757 from: 1 to: 5668 pFN21AB8263 5668 bp With 182 enzymes: SgfI * February 27, 2009 14:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3478 278 Size: 53 83 186 936 1942 2468 Fragments arranged by size: 2468 1942 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3011 1937 Size: 1074 4594 Acc65I G'GTAC_C Cuts at: 2989 2989 Size: 5668 AclI AA'CG_TT Cuts at: 1563 2967 3795 4168 1563 Size: 1404 828 373 3063 Fragments arranged by size: 3063 1404 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3731 5115 5383 1632 Size: 2099 1384 268 1917 Fragments arranged by size: 2099 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5471 AflIII A'CryG_T Cuts at: 1203 4567 5544 1203 Size: 3364 977 1327 Fragments arranged by size: 3364 1327 977 AgeI A'CCGG_T Cuts at: 4499 4499 Size: 5668 AhdI GACnn_n'nnGTC Cuts at: 4397 4397 Size: 5668 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5668 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5668 AlwNI CAG_nnn'CTG Cuts at: 3411 4983 3411 Size: 1572 4096 ApaLI G'TGCA_C Cuts at: 3725 4881 3725 Size: 1156 4512 ApoI r'AATT_y Cuts at: 1493 2977 3203 3355 5464 1493 Size: 1484 226 152 2109 1697 Fragments arranged by size: 2109 1697 1484 226 152 AseI AT'TA_AT Cuts at: 160 1032 4222 160 Size: 872 3190 1606 Fragments arranged by size: 3190 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5668 AvaI C'yCGr_G Cuts at: 1870 1943 2501 2512 2993 1870 Size: 73 558 11 481 4545 Fragments arranged by size: 4545 558 481 73 11 BamHI G'GATC_C Cuts at: 2998 3365 2998 Size: 367 5301 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2516 2989 4444 618 Size: 325 217 627 729 473 1455 1842 Fragments arranged by size: 1842 1455 729 627 473 325 217 BanII G_rGCy'C Cuts at: 1391 2987 1391 Size: 1596 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2284 928 Size: 971 385 4312 Fragments arranged by size: 4312 971 385 BbvCI CC'TCA_GC Cuts at: 2824 2824 Size: 5668 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2665 2699 3859 3893 1903 Size: 34 728 34 1160 34 3678 Fragments arranged by size: 3678 1160 728 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3560 4770 3560 Size: 1210 4458 BclI T'GATC_A Cuts at: 1548 1548 Size: 5668 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2052 2088 2278 Size: 107 122 71 631 605 380 36 190 Cuts at: 2278 4279 136 Size: 2001 1525 Fragments arranged by size: 2001 1525 631 605 380 190 122 107 71 36 BglII A'GATC_T Cuts at: 5663 5663 Size: 5668 BlpI GC'TnA_GC Cuts at: 3085 3085 Size: 5668 Bme1580I G_kGCm'C Cuts at: 1790 2519 3729 4885 1790 Size: 729 1210 1156 2573 Fragments arranged by size: 2573 1210 1156 729 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5668 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3399 4357 442 Size: 416 53 83 399 24 1982 958 1753 Fragments arranged by size: 1982 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2438 1056 Size: 1382 4286 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4328 1239 Size: 3089 2579 Bpu10I CC'TnA_GC Cuts at: 2487 2824 2487 Size: 337 5331 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2921 3793 4658 4956 2921 Size: 872 865 298 3633 Fragments arranged by size: 3633 872 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2530 2910 4331 882 Size: 617 1031 380 1421 2219 Fragments arranged by size: 2219 1421 1031 617 380 BsaAI yAC'GTr Cuts at: 493 1204 3459 493 Size: 711 2255 2702 Fragments arranged by size: 2702 2255 711 BsaBI GATnn'nnATC Cuts at: 2471 5662 2471 Size: 3191 2477 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3475 3857 4492 275 Size: 53 83 186 936 1942 382 635 1451 Fragments arranged by size: 1942 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2551 4101 4499 4773 4920 5331 2551 Size: 1550 398 274 147 411 2888 Fragments arranged by size: 2888 1550 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5443 Fragments arranged by size: 5443 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2154 2157 2184 2187 1186 Size: 968 3 27 3 4667 Fragments arranged by size: 4667 968 27 3 3 BseYI C'CCAG_C Cuts at: 2718 4871 5367 2718 Size: 2153 496 3019 Fragments arranged by size: 3019 2153 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5095 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2047 3118 3879 4907 1540 Size: 275 186 46 1071 761 1028 2301 Fragments arranged by size: 2301 1071 1028 761 275 186 46 BsiHKAI G_wGCw'C Cuts at: 3729 3814 4885 3729 Size: 85 1071 4512 Fragments arranged by size: 4512 1071 85 BsmI GAATG_Cn' Cuts at: 2755 3179 3272 2755 Size: 424 93 5151 Fragments arranged by size: 5151 424 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2519 2987 3729 3814 4885 1391 Size: 399 729 468 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 729 468 399 85 BspEI T'CCGG_A Cuts at: 2551 5331 2551 Size: 2780 2888 BspHI T'CATG_A Cuts at: 3557 5287 3557 Size: 1730 3938 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3023 844 Size: 2179 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2427 2589 3555 1394 Size: 429 604 162 966 3507 Fragments arranged by size: 3507 966 604 429 162 BsrDI GCAATG_nn' Cuts at: 65 1248 2694 4163 4337 65 Size: 1183 1446 1469 174 1396 Fragments arranged by size: 1469 1446 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4312 4499 1700 Size: 110 2502 187 2869 Fragments arranged by size: 2869 2502 187 110 BsrGI T'GTAC_A Cuts at: 96 2940 96 Size: 2844 2824 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5668 BssSI C'ACGA_G Cuts at: 3728 4740 3728 Size: 1012 4656 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5668 BstBI TT'CG_AA Cuts at: 4472 4472 Size: 5668 BstEII G'GTnAC_C Cuts at: 1184 2060 2323 1184 Size: 876 263 4529 Fragments arranged by size: 4529 876 263 BstXI CCAn_nnnn'nTGG Cuts at: 2204 2335 2591 2204 Size: 131 256 5281 Fragments arranged by size: 5281 256 131 BstYI r'GATC_y Cuts at: 1972 2466 2998 3365 3750 3767 5219 5305 5317 Size: 494 532 367 385 17 1452 86 12 Cuts at: 5317 5663 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 532 494 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2026 2065 2520 2925 513 Size: 552 961 39 455 405 3256 Fragments arranged by size: 3256 961 552 455 405 39 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2668 487 Size: 683 56 1442 3487 Fragments arranged by size: 3487 1442 683 56 BtsI GCAGTG_nn' Cuts at: 3184 3978 3998 3184 Size: 794 20 4854 Fragments arranged by size: 4854 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5668 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5633 DraI TTT'AAA Cuts at: 2972 2972 Size: 5668 DraIII CAC_nnn'GTG Cuts at: 2785 2785 Size: 5668 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4675 809 Size: 507 3359 1802 Fragments arranged by size: 3359 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2044 2593 2613 Size: 54 1425 50 275 18 214 549 20 Cuts at: 2613 3115 4004 5633 8 Size: 502 889 1629 43 Fragments arranged by size: 1629 1425 889 549 502 275 214 54 50 43 20 18 EagI C'GGCC_G Cuts at: 1537 1812 2044 3115 1537 Size: 275 232 1071 4090 Fragments arranged by size: 4090 1071 275 232 EarI CTCTTCn'nnn_ Cuts at: 1353 2173 2360 2615 3598 1353 Size: 820 187 255 983 3423 Fragments arranged by size: 3423 983 820 255 187 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2130 4255 4629 4775 387 Size: 1064 138 541 2125 374 146 1280 Fragments arranged by size: 2125 1280 1064 541 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3731 4328 5115 5383 1239 Size: 393 2099 597 787 268 1524 Fragments arranged by size: 2099 1524 787 597 393 268 EcoRI G'AATT_C Cuts at: 2977 3355 2977 Size: 378 5290 FspI TGC'GCA Cuts at: 4174 4174 Size: 5668 HaeII r_GCGC'y Cuts at: 1678 4815 1678 Size: 3137 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4309 4341 4383 4415 1373 Size: 32 135 32 2737 32 42 32 2626 Fragments arranged by size: 2737 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2895 3012 3258 669 Size: 988 281 957 117 246 3079 Fragments arranged by size: 3079 988 957 281 246 117 HindIII A'AGCT_T Cuts at: 748 748 Size: 5668 HpaI GTT'AAC Cuts at: 3258 3258 Size: 5668 KpnI G_GTAC'C Cuts at: 2993 2993 Size: 5668 MfeI C'AATT_G Cuts at: 3267 3267 Size: 5668 MluI A'CGCG_T Cuts at: 5544 5544 Size: 5668 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4782 4966 1305 Size: 3477 184 2007 Fragments arranged by size: 3477 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2615 10 Size: 54 1425 343 783 3063 Fragments arranged by size: 3063 1425 783 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2202 2280 2286 2639 2687 3626 3985 Size: 648 1036 78 6 353 48 939 359 Cuts at: 3985 4144 518 Size: 159 2042 Fragments arranged by size: 2042 1036 939 648 359 353 159 78 48 6 MspA1I CmG'CkG Cuts at: 1606 3084 3761 4909 5154 1606 Size: 1478 677 1148 245 2120 Fragments arranged by size: 2120 1478 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5116 NdeI CA'TA_TG Cuts at: 387 2908 387 Size: 2521 3147 NheI G'CTAG_C Cuts at: 1052 2434 1052 Size: 1382 4286 NotI GC'GGCC_GC Cuts at: 3115 3115 Size: 5668 NspI r_CATG'y Cuts at: 1132 1229 2680 3026 4571 1132 Size: 97 1451 346 1545 2229 Fragments arranged by size: 2229 1545 1451 346 97 PasI CC'CwG_GG Cuts at: 1348 2342 1348 Size: 994 4674 PciI A'CATG_T Cuts at: 4567 4567 Size: 5668 PflMI CCAn_nnn'nTGG Cuts at: 1108 2926 1108 Size: 1818 3850 PmeI GTTT'AAAC Cuts at: 2972 2972 Size: 5668 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3737 3769 5250 5282 3737 Size: 32 1481 32 4123 Fragments arranged by size: 4123 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5668 PsiI TTA'TAA Cuts at: 3238 3238 Size: 5668 PstI C_TGCA'G Cuts at: 830 3020 830 Size: 2190 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5668 SalI G'TCGA_C Cuts at: 1936 3010 1936 Size: 1074 4594 SbfI CC_TGCA'GG Cuts at: 3020 3020 Size: 5668 ScaI AGT'ACT Cuts at: 3916 5628 3916 Size: 1712 3956 SexAI A'CCwGG_T Cuts at: 2334 2334 Size: 5668 SfcI C'TryA_G Cuts at: 826 1045 3016 4151 4832 5023 826 Size: 219 1971 1135 681 191 1471 Fragments arranged by size: 1971 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5668 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5668 SmaI CCC'GGG Cuts at: 1872 2995 1872 Size: 1123 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2900 3772 4673 4935 820 Size: 197 926 957 872 901 262 1553 Fragments arranged by size: 1553 957 926 901 872 262 197 SnaBI TAC'GTA Cuts at: 493 3459 493 Size: 2966 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5668 SphI G_CATG'C Cuts at: 1132 2680 3026 1132 Size: 1548 346 3774 Fragments arranged by size: 3774 1548 346 SspI AAT'ATT Cuts at: 5 52 3592 5 Size: 47 3540 2081 Fragments arranged by size: 3540 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5668 StyI C'CwwG_G Cuts at: 513 1065 2955 3107 513 Size: 552 1890 152 3074 Fragments arranged by size: 3074 1890 552 152 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2441 2676 3695 3712 3865 4050 4495 Size: 1255 861 235 1019 17 153 185 445 Cuts at: 4495 325 Size: 1498 Fragments arranged by size: 1498 1255 1019 861 445 235 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2940 3914 5626 Size: 274 80 33 51 1443 963 974 1712 Cuts at: 5626 96 Size: 138 Fragments arranged by size: 1712 1443 974 963 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2769 2801 5628 5660 2769 Size: 32 2827 32 2777 Fragments arranged by size: 2827 2777 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5668 XbaI T'CTAG_A Cuts at: 3004 3004 Size: 5668 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5668 XmaI C'CCGG_G Cuts at: 1870 2993 1870 Size: 1123 4545 XmnI GAAnn'nnTTC Cuts at: 2420 3797 2420 Size: 1377 4291 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3476 276 Size: 53 83 186 936 1942 2468 Fragments arranged by size: 2468 1942 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI