Restriction map for
pFN21AB8255
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# NO CUT ENZYMES ( 152 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI AocI Aor51HI ApaI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaII AviII AvrII BanII
BbeI BbrPI BbvCI BcgI BciVI
BclI BfrI BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaWI BseAI
BsgI BsiEI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrGI BssHII Bst1107I BstAPI
BstBI BstUI BstXI BstZ17I Bsu36I
CelII ClaI CpoI DraI DsaI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoRV EcoT22I
FbaI FseI FspI HaeII HgaI
Hin1I HincII HindII HindIII HpaI
KasI KpnI Ksp632I KspI MaeII
MamI MfeI MluI MroI MseI
MspA1I MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TfiI
Tru9I Tth111I XbaI XcmI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AcsI 158 GAATTC [GA]^AATT[CT]
AfaI 827 GTAC GT^AC
Alw44I 855 GTGCAC G^TGCAC
AlwNI 886 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 855 GTGCAC G^TGCAC
ApoI 158 GAATTC [GA]^AATT[CT]
BamHI 610 GGATCC G^GATCC
BbsI 552 GAAGAC GAAGAC(2/6)
BcnI 81 CCCGG CC^[GC]GG
BfaI 688 CTAG C^TAG
BglII 770 AGATCT A^GATCT
BpuAI 552 GAAGAC GAAGAC(2/6)
BsaI 145 GAGACC GGTCTC(1/5)
BsmBI 495 CGTCTC CGTCTC(1/5)
BspMI 930 GCAGGT ACCTGC(4/8)
BsrFI 350 ACCGGC [GA]^CCGG[CT]
BssSI 291 CACGAG CACGAG(-5/-1)
CfoI 261 GCGC GCG^C
Cfr10I 350 ACCGGC [GA]^CCGG[CT]
DraII 756 AGGCCCC [GA]G^G[ACGT]CC[CT]
DraIII 164 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 511 GACAAGCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 756 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRI 158 GAATTC G^AATTC
EcoT14I 314 CCAAGG C^C[AT][AT]GG
HhaI 261 GCGC GCG^C
HinP1I 261 GCGC G^CGC
HinfI 888 GACTC G^A[ACGT]TC
MaeI 688 CTAG C^TAG
MboII 552 GAAGA GAAGA(8/7)
NciI 81 CCCGG CC^[GC]GG
PflMI 647 CCATGTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 888 GACTC GAGTC(4/5)
RsaI 827 GTAC GT^AC
SexAI 914 ACCTGGT A^CC[AT]GGT
SfaNI 764 GCATC GCATC(5/9)
StyI 314 CCAAGG C^C[AT][AT]GG
Tsp509I 159 AATT ^AATT
Van91I 647 CCATGTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AspHI 170 GTGCTC G[AT]GC[AT]^C
855 GTGCAC
AvaI 104 CTCGAG C^[CT]CG[GA]G
371 CTCGAG
BanI 25 GGCACC G^G[CT][GA]CC
283 GGCACC
BglI 38 GCCACAACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
569 GCCACACAGGC
BpmI 602 CTCCAG CTGGAG(16/14)
626 CTGGAG
BseRI 213 CTCCTC GAGGAG(10/8)
339 CTCCTC
BsiHKAI 170 GTGCTC G[AT]GC[AT]^C
855 GTGCAC
BsmFI 509 GGGAC GGGAC(10/14)
698 GGGAC
BsoBI 104 CTCGAG C^[CT]CG[GA]G
371 CTCGAG
BstEII 51 GGTAACC G^GT[ACGT]ACC
933 GGTGACC
BstPI 51 GGTAACC G^GT[ACGT]ACC
933 GGTGACC
EcoO65I 51 GGTAACC G^GT[ACGT]ACC
933 GGTGACC
FauI 72 CCCGC CCCGC(4/6)
177 GCGGG
HapII 82 CCGG C^CGG
351 CCGG
HpaII 82 CCGG C^CGG
351 CCGG
HphI 591 GGTGA GGTGA(8/7)
933 GGTGA
MslI 117 CATCATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
848 CACCCCAGTG
MspI 82 CCGG C^CGG
351 CCGG
PaeR7I 104 CTCGAG C^TCGAG
371 CTCGAG
TaqI 105 TCGA T^CGA
372 TCGA
TspRI 420 CACTG CA[GC]TG[ACGT][ACGT]^
853 CAGTG
TthHB8I 105 TCGA T^CGA
372 TCGA
XhoI 104 CTCGAG C^TCGAG
371 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AciI AluI AlwI BalI BbvI
BmyI BsaJI BsiYI BslI BsmAI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
EaeI EcoRII Fnu4HI FokI HaeIII
ItaI MaeIII MboI MflI MluNI
MnlI MscI MvaI MwoI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
SmlI TseI Tsp45I XhoII