Restriction map for
pFN21AB8255
(Linear) (Six-base) MAPSORT of: KIBB8255.seq Check: 734 from: 1 to: 951
KIBB8255.orf
With 182 enzymes: SgfI *
March 3, 2008 11:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 921 951
Size: 921 30
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 571 705 829 951
Size: 571 134 124 122
Fragments arranged by size:
571 134 124 122
AleI CACnn'nnGTG
Cuts at: 0 852 951
Size: 852 99
AlwNI CAG_nnn'CTG
Cuts at: 0 891 951
Size: 891 60
ApaLI G'TGCA_C
Cuts at: 0 855 951
Size: 855 96
ApoI r'AATT_y
Cuts at: 0 158 951
Size: 158 793
AvaI C'yCGr_G
Cuts at: 0 104 371 951
Size: 104 267 580
Fragments arranged by size:
580 267 104
BamHI G'GATC_C
Cuts at: 0 610 951
Size: 610 341
BanI G'GyrC_C
Cuts at: 0 25 283 951
Size: 25 258 668
Fragments arranged by size:
668 258 25
BbsI GAAGACnn'nnnn_
Cuts at: 0 559 951
Size: 559 392
BglI GCCn_nnn'nGGC
Cuts at: 0 44 575 951
Size: 44 531 376
Fragments arranged by size:
531 376 44
BglII A'GATC_T
Cuts at: 0 770 951
Size: 770 181
Bme1580I G_kGCm'C
Cuts at: 0 286 859 951
Size: 286 573 92
Fragments arranged by size:
573 286 92
BmrI ACTGGGnnnn_n'
Cuts at: 0 846 951
Size: 846 105
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 456 951
Size: 456 495
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 587 647 951
Size: 587 60 304
Fragments arranged by size:
587 304 60
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 16 951
Size: 16 935
BsaI GGTCTCn'nnnn_
Cuts at: 0 139 951
Size: 139 812
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 585 615 951
Size: 585 30 336
Fragments arranged by size:
585 336 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 204 330 951
Size: 204 126 621
Fragments arranged by size:
621 204 126
BseYI C'CCAG_C
Cuts at: 0 47 506 760 951
Size: 47 459 254 191
Fragments arranged by size:
459 254 191 47
BsiHKAI G_wGCw'C
Cuts at: 0 174 859 951
Size: 174 685 92
Fragments arranged by size:
685 174 92
BsmBI CGTCTCn'nnnn_
Cuts at: 0 501 951
Size: 501 450
Bsp1286I G_dGCh'C
Cuts at: 0 174 286 859 951
Size: 174 112 573 92
Fragments arranged by size:
573 174 112 92
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 921 951
Size: 921 30
BsrFI r'CCGG_y
Cuts at: 0 350 951
Size: 350 601
BssSI C'ACGA_G
Cuts at: 0 291 951
Size: 291 660
BstEII G'GTnAC_C
Cuts at: 0 51 933 951
Size: 51 882 18
Fragments arranged by size:
882 51 18
BstYI r'GATC_y
Cuts at: 0 270 367 610 657 770 951
Size: 270 97 243 47 113 181
Fragments arranged by size:
270 243 181 113 97 47
DraIII CAC_nnn'GTG
Cuts at: 0 169 951
Size: 169 782
DrdI GACnn_nn'nnGTC
Cuts at: 0 517 951
Size: 517 434
EaeI y'GGCC_r
Cuts at: 0 222 567 639 882 951
Size: 222 345 72 243 69
Fragments arranged by size:
345 243 222 72 69
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 87 951
Size: 87 864
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 571 587 647 705 829 951
Size: 571 16 60 58 124 122
Fragments arranged by size:
571 124 122 60 58 16
EcoO109I rG'GnC_Cy
Cuts at: 0 757 951
Size: 757 194
EcoRI G'AATT_C
Cuts at: 0 158 951
Size: 158 793
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 436 468 585 617 951
Size: 436 32 117 32 334
Fragments arranged by size:
436 334 117 32 32
MscI TGG'CCA
Cuts at: 0 224 569 641 884 951
Size: 224 345 72 243 67
Fragments arranged by size:
345 243 224 72 67
MslI CAynn'nnrTG
Cuts at: 0 121 852 951
Size: 121 731 99
Fragments arranged by size:
731 121 99
PasI CC'CwG_GG
Cuts at: 0 402 951
Size: 402 549
PflMI CCAn_nnn'nTGG
Cuts at: 0 653 951
Size: 653 298
PfoI T'CCnGG_A
Cuts at: 0 80 425 951
Size: 80 345 526
Fragments arranged by size:
526 345 80
SexAI A'CCwGG_T
Cuts at: 0 914 951
Size: 914 37
SmlI C'TyrA_G
Cuts at: 0 31 104 371 951
Size: 31 73 267 580
Fragments arranged by size:
580 267 73 31
StyI C'CwwG_G
Cuts at: 0 314 951
Size: 314 637
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 394 438 951
Size: 394 44 513
Fragments arranged by size:
513 394 44
XhoI C'TCGA_G
Cuts at: 0 104 371 951
Size: 104 267 580
Fragments arranged by size:
580 267 104
Enzymes that do cut:
AarI AcuI AleI AlwNI ApaLI ApoI AvaI BamHI
BanI BbsI BglI BglII Bme1580I BmrI BplI BpmI
BpuEI BsaI BsaXI BseRI BseYI BsiHKAI BsmBI Bsp1286I
BspMI BsrFI BssSI BstEII BstYI DraIII DrdI EaeI
EciI Eco57MI EcoO109I EcoRI Hin4I MscI MslI PasI
PflMI PfoI SexAI SmlI StyI TaqII XhoI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaI AscI AseI AsiSI AvrII
BaeI BanII BbeI BbvCI BcgI BciVI BclI BfrBI
BlpI BmgBI BmtI Bpu10I BsaAI BsaBI BsaHI BsaWI
BsgI BsiEI BsiWI BsmI BspEI BspHI BsrBI BsrDI
BsrGI BssHII BstAPI BstBI BstXI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI EagI EarI EcoICRI
EcoNI EcoRV FalI FseI FspI FspAI HaeII HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PciI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI TatI TstI Tth111I
XbaI XcmI XmaI XmnI ZraI