(Circular) (Six-base) MAPSORT of: pFN21AB8252.seq Check: 2052 from: 1 to: 5710 pFN21AB8252 5710 bp With 182 enzymes: SgfI * February 27, 2009 14:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3520 278 Size: 53 83 186 936 1984 2468 Fragments arranged by size: 2468 1984 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3053 1937 Size: 1116 4594 Acc65I G'GTAC_C Cuts at: 3031 3031 Size: 5710 AclI AA'CG_TT Cuts at: 1563 3837 4210 1563 Size: 2274 373 3063 Fragments arranged by size: 3063 2274 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3773 5157 5425 1632 Size: 2141 1384 268 1917 Fragments arranged by size: 2141 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5513 AflIII A'CryG_T Cuts at: 1203 2139 2721 4609 5586 1203 Size: 936 582 1888 977 1327 Fragments arranged by size: 1888 1327 977 936 582 AgeI A'CCGG_T Cuts at: 4541 4541 Size: 5710 AhdI GACnn_n'nnGTC Cuts at: 2745 4439 2745 Size: 1694 4016 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5710 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5710 AlwNI CAG_nnn'CTG Cuts at: 3453 5025 3453 Size: 1572 4138 ApaI G_GGCC'C Cuts at: 2810 2810 Size: 5710 ApaLI G'TGCA_C Cuts at: 3767 4923 3767 Size: 1156 4554 ApoI r'AATT_y Cuts at: 1493 2168 2954 3019 3245 3397 5506 1493 Size: 675 786 65 226 152 2109 1697 Fragments arranged by size: 2109 1697 786 675 226 152 65 AseI AT'TA_AT Cuts at: 160 1032 4264 160 Size: 872 3232 1606 Fragments arranged by size: 3232 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5710 AvaI C'yCGr_G Cuts at: 1870 1943 3035 1870 Size: 73 1092 4545 Fragments arranged by size: 4545 1092 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2494 2527 2494 Size: 33 5677 BamHI G'GATC_C Cuts at: 3040 3407 3040 Size: 367 5343 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2049 2177 3031 4486 618 Size: 325 217 627 262 128 854 1455 1842 Fragments arranged by size: 1842 1455 854 627 325 262 217 128 BanII G_rGCy'C Cuts at: 1391 2335 2611 2810 3029 1391 Size: 944 276 199 219 4072 Fragments arranged by size: 4072 944 276 219 199 BbeI G_GCGC'C Cuts at: 2053 2053 Size: 5710 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2299 2567 928 Size: 971 400 268 4071 Fragments arranged by size: 4071 971 400 268 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3901 3935 1903 Size: 34 1964 34 3678 Fragments arranged by size: 3678 1964 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3602 4812 3602 Size: 1210 4500 BclI T'GATC_A Cuts at: 1548 1548 Size: 5710 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2996 4321 136 Size: 107 122 71 631 605 1324 1325 1525 Fragments arranged by size: 1525 1325 1324 631 605 122 107 71 BglII A'GATC_T Cuts at: 5705 5705 Size: 5710 BlpI GC'TnA_GC Cuts at: 2473 3127 2473 Size: 654 5056 Bme1580I G_kGCm'C Cuts at: 1790 2810 3771 4927 1790 Size: 1020 961 1156 2573 Fragments arranged by size: 2573 1156 1020 961 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5710 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3441 4399 442 Size: 416 53 83 399 24 2024 958 1753 Fragments arranged by size: 2024 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5710 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2597 4370 1239 Size: 1358 1773 2579 Fragments arranged by size: 2579 1773 1358 Bpu10I CC'TnA_GC Cuts at: 2670 2670 Size: 5710 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2420 2798 3835 4700 4998 2420 Size: 378 1037 865 298 3132 Fragments arranged by size: 3132 1037 865 378 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2854 4373 882 Size: 617 1355 1519 2219 Fragments arranged by size: 2219 1519 1355 617 BsaAI yAC'GTr Cuts at: 493 1204 3501 493 Size: 711 2297 2702 Fragments arranged by size: 2702 2297 711 BsaBI GATnn'nnATC Cuts at: 5704 5704 Size: 5710 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2050 2304 2798 3517 Size: 53 83 186 936 517 254 494 719 Cuts at: 3517 3899 4534 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 719 635 517 494 382 254 186 83 53 BsaWI w'CCGG_w Cuts at: 4143 4541 4815 4962 5373 4143 Size: 398 274 147 411 4480 Fragments arranged by size: 4480 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2861 2891 2984 3014 1178 Size: 30 165 30 1458 30 93 30 3874 Fragments arranged by size: 3874 1458 165 93 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2842 2869 1186 Size: 1656 27 4027 Fragments arranged by size: 4027 1656 27 BseYI C'CCAG_C Cuts at: 2327 2894 4913 5409 2327 Size: 567 2019 496 2628 Fragments arranged by size: 2628 2019 567 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2443 1163 Size: 573 707 4430 Fragments arranged by size: 4430 707 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3160 3921 4949 1540 Size: 275 186 1159 761 1028 2301 Fragments arranged by size: 2301 1159 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2841 3771 3856 4927 2841 Size: 930 85 1071 3624 Fragments arranged by size: 3624 1071 930 85 BsmI GAATG_Cn' Cuts at: 3221 3314 3221 Size: 93 5617 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2335 2611 2810 2841 3029 3771 3856 Size: 399 545 276 199 31 188 742 85 Cuts at: 3856 4927 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 545 399 276 199 188 85 31 BspEI T'CCGG_A Cuts at: 5373 5373 Size: 5710 BspHI T'CATG_A Cuts at: 2526 3599 5329 2526 Size: 1073 1730 2907 Fragments arranged by size: 2907 1730 1073 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2750 2767 3065 844 Size: 1906 17 298 3489 Fragments arranged by size: 3489 1906 298 17 BsrBI CCG'CTC Cuts at: 1394 1823 3597 1394 Size: 429 1774 3507 Fragments arranged by size: 3507 1774 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2455 2904 4205 4379 65 Size: 1183 1207 449 1301 174 1396 Fragments arranged by size: 1396 1301 1207 1183 449 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4354 4541 1700 Size: 110 2544 187 2869 Fragments arranged by size: 2869 2544 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5710 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5710 BssSI C'ACGA_G Cuts at: 3770 4782 3770 Size: 1012 4698 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5710 BstBI TT'CG_AA Cuts at: 4514 4514 Size: 5710 BstEII G'GTnAC_C Cuts at: 1184 2056 1184 Size: 872 4838 BstXI CCAn_nnnn'nTGG Cuts at: 2653 2653 Size: 5710 BstYI r'GATC_y Cuts at: 1972 2245 3040 3407 3792 3809 5261 5347 5359 Size: 273 795 367 385 17 1452 86 12 Cuts at: 5359 5705 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 795 385 367 346 273 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2161 2173 2316 2602 513 Size: 552 939 157 12 143 286 3621 Fragments arranged by size: 3621 939 552 286 157 143 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2283 487 Size: 683 56 1057 3914 Fragments arranged by size: 3914 1057 683 56 BtsI GCAGTG_nn' Cuts at: 2547 2712 3226 4020 4040 2547 Size: 165 514 794 20 4217 Fragments arranged by size: 4217 794 514 165 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5710 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5675 DraI TTT'AAA Cuts at: 3014 3014 Size: 5710 DraIII CAC_nnn'GTG Cuts at: 2845 2845 Size: 5710 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2924 4717 809 Size: 507 1608 1793 1802 Fragments arranged by size: 1802 1793 1608 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2484 2599 3157 Size: 54 1425 50 275 18 654 115 558 Cuts at: 3157 4046 5675 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 654 558 275 115 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3157 1537 Size: 275 1345 4090 Fragments arranged by size: 4090 1345 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3640 1353 Size: 2287 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4297 4671 4817 387 Size: 1064 138 2708 374 146 1280 Fragments arranged by size: 2708 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2597 3773 4370 5157 5425 1239 Size: 393 965 1176 597 787 268 1524 Fragments arranged by size: 1524 1176 965 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2486 2486 Size: 5710 EcoO109I rG'GnC_Cy Cuts at: 2806 2807 2806 Size: 1 5709 EcoRI G'AATT_C Cuts at: 3019 3397 3019 Size: 378 5332 FspI TGC'GCA Cuts at: 4216 4216 Size: 5710 HaeII r_GCGC'y Cuts at: 1678 2053 2280 2643 4857 1678 Size: 375 227 363 2214 2531 Fragments arranged by size: 2531 2214 375 363 227 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2561 2593 2731 2763 2861 Size: 32 135 32 989 32 138 32 98 Cuts at: 2861 2893 4351 4383 4425 4457 1373 Size: 32 1458 32 42 32 2626 Fragments arranged by size: 2626 1458 989 138 135 98 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2029 3054 3300 669 Size: 988 281 91 1025 246 3079 Fragments arranged by size: 3079 1025 988 281 246 91 HindIII A'AGCT_T Cuts at: 748 2201 748 Size: 1453 4257 HpaI GTT'AAC Cuts at: 3300 3300 Size: 5710 KasI G'GCGC_C Cuts at: 2049 2049 Size: 5710 KpnI G_GTAC'C Cuts at: 3035 3035 Size: 5710 MfeI C'AATT_G Cuts at: 3309 3309 Size: 5710 MluI A'CGCG_T Cuts at: 5586 5586 Size: 5710 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2002 2781 4824 5008 1305 Size: 697 779 2043 184 2007 Fragments arranged by size: 2043 2007 779 697 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2486 10 Size: 54 1425 343 654 3234 Fragments arranged by size: 3234 1425 654 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2315 2405 2693 3668 4027 4186 518 Size: 648 1149 90 288 975 359 159 2042 Fragments arranged by size: 2042 1149 975 648 359 288 159 90 MspA1I CmG'CkG Cuts at: 1606 2604 3126 3803 4951 5196 1606 Size: 998 522 677 1148 245 2120 Fragments arranged by size: 2120 1148 998 677 522 245 NarI GG'CG_CC Cuts at: 2050 2050 Size: 5710 NcoI C'CATG_G Cuts at: 513 1065 2004 2161 2173 2316 513 Size: 552 939 157 12 143 3907 Fragments arranged by size: 3907 939 552 157 143 12 NdeI CA'TA_TG Cuts at: 387 387 Size: 5710 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5710 NotI GC'GGCC_GC Cuts at: 3157 3157 Size: 5710 NspI r_CATG'y Cuts at: 1132 1229 2143 3068 4613 1132 Size: 97 914 925 1545 2229 Fragments arranged by size: 2229 1545 925 914 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5710 PciI A'CATG_T Cuts at: 2139 4609 2139 Size: 2470 3240 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5710 PfoI T'CCnGG_A Cuts at: 2235 2579 2235 Size: 344 5366 PmeI GTTT'AAAC Cuts at: 3014 3014 Size: 5710 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3779 3811 5292 5324 3779 Size: 32 1481 32 4165 Fragments arranged by size: 4165 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2534 1613 Size: 921 4789 PsiI TTA'TAA Cuts at: 3280 3280 Size: 5710 PspOMI G'GGCC_C Cuts at: 2806 2806 Size: 5710 PstI C_TGCA'G Cuts at: 830 3062 830 Size: 2232 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5710 SacII CC_GC'GG Cuts at: 2605 2605 Size: 5710 SalI G'TCGA_C Cuts at: 1936 3052 1936 Size: 1116 4594 SbfI CC_TGCA'GG Cuts at: 3062 3062 Size: 5710 ScaI AGT'ACT Cuts at: 3958 5670 3958 Size: 1712 3998 SexAI A'CCwGG_T Cuts at: 2841 2841 Size: 5710 SfcI C'TryA_G Cuts at: 826 1045 2962 3058 4193 4874 5065 826 Size: 219 1917 96 1135 681 191 1471 Fragments arranged by size: 1917 1471 1135 681 219 191 96 SfiI GGCCn_nnn'nGGCC Cuts at: 2996 2996 Size: 5710 SfoI GGC'GCC Cuts at: 2051 2051 Size: 5710 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5710 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5710 SmaI CCC'GGG Cuts at: 1872 3037 1872 Size: 1165 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2435 2813 3814 4715 4977 820 Size: 197 926 492 378 1001 901 262 1553 Fragments arranged by size: 1553 1001 926 901 492 378 262 197 SnaBI TAC'GTA Cuts at: 493 3501 493 Size: 3008 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5710 SphI G_CATG'C Cuts at: 1132 3068 1132 Size: 1936 3774 SspI AAT'ATT Cuts at: 5 52 3634 5 Size: 47 3582 2081 Fragments arranged by size: 3582 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5710 StyI C'CwwG_G Cuts at: 513 1065 2004 2161 2173 2316 2487 2646 3003 Size: 552 939 157 12 143 171 159 357 Cuts at: 3003 3149 513 Size: 146 3074 Fragments arranged by size: 3074 939 552 357 171 159 157 146 143 12 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2174 2393 2570 2888 3737 3754 3907 Size: 1255 594 219 177 318 849 17 153 Cuts at: 3907 4092 4537 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 849 594 445 318 219 185 177 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3956 5668 96 Size: 274 80 33 51 1443 1979 1712 138 Fragments arranged by size: 1979 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2984 3016 5670 5702 2984 Size: 32 2654 32 2992 Fragments arranged by size: 2992 2654 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5710 XbaI T'CTAG_A Cuts at: 2749 3046 2749 Size: 297 5413 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2398 2504 2725 2398 Size: 106 221 5383 Fragments arranged by size: 5383 221 106 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5710 XmaI C'CCGG_G Cuts at: 1870 3035 1870 Size: 1165 4545 XmnI GAAnn'nnTTC Cuts at: 3839 3839 Size: 5710 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3518 276 Size: 53 83 186 936 1984 2468 Fragments arranged by size: 2468 1984 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacII SalI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SanDI SapI SrfI SwaI