(Circular) (Six-base) MAPSORT of: pFN21AB8248.seq Check: 7490 from: 1 to: 5410 pFN21AB8248 5410 bp With 182 enzymes: SgfI * February 27, 2009 14:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2426 2426 Size: 5410 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3220 278 Size: 53 83 186 936 1684 2468 Fragments arranged by size: 2468 1684 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2753 1937 Size: 816 4594 Acc65I G'GTAC_C Cuts at: 2731 2731 Size: 5410 AclI AA'CG_TT Cuts at: 1563 3537 3910 1563 Size: 1974 373 3063 Fragments arranged by size: 3063 1974 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3473 4857 5125 1632 Size: 1841 1384 268 1917 Fragments arranged by size: 1917 1841 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5213 AflIII A'CryG_T Cuts at: 1203 2304 2659 4309 5286 1203 Size: 1101 355 1650 977 1327 Fragments arranged by size: 1650 1327 1101 977 355 AgeI A'CCGG_T Cuts at: 4241 4241 Size: 5410 AhdI GACnn_n'nnGTC Cuts at: 4139 4139 Size: 5410 AleI CACnn'nnGTG Cuts at: 1166 2357 1166 Size: 1191 4219 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5410 AlwNI CAG_nnn'CTG Cuts at: 2113 2615 2632 3153 4725 2113 Size: 502 17 521 1572 2798 Fragments arranged by size: 2798 1572 521 502 17 ApaLI G'TGCA_C Cuts at: 3467 4623 3467 Size: 1156 4254 ApoI r'AATT_y Cuts at: 1493 2719 2945 3097 5206 1493 Size: 1226 226 152 2109 1697 Fragments arranged by size: 2109 1697 1226 226 152 AseI AT'TA_AT Cuts at: 160 1032 3964 160 Size: 872 2932 1606 Fragments arranged by size: 2932 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5410 AvaI C'yCGr_G Cuts at: 1870 1943 2067 2535 2735 1870 Size: 73 124 468 200 4545 Fragments arranged by size: 4545 468 200 124 73 BamHI G'GATC_C Cuts at: 2506 2740 3107 2506 Size: 234 367 4809 Fragments arranged by size: 4809 367 234 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2731 4186 618 Size: 325 217 627 944 1455 1842 Fragments arranged by size: 1842 1455 944 627 325 217 BanII G_rGCy'C Cuts at: 1391 2455 2604 2622 2729 1391 Size: 1064 149 18 107 4072 Fragments arranged by size: 4072 1064 149 107 18 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2656 928 Size: 971 757 3682 Fragments arranged by size: 3682 971 757 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3601 3635 1903 Size: 34 1664 34 3678 Fragments arranged by size: 3678 1664 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3302 4512 3302 Size: 1210 4200 BclI T'GATC_A Cuts at: 1548 2295 1548 Size: 747 4663 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2420 4021 136 Size: 107 122 71 631 605 748 1601 1525 Fragments arranged by size: 1601 1525 748 631 605 122 107 71 BglII A'GATC_T Cuts at: 5405 5405 Size: 5410 BlpI GC'TnA_GC Cuts at: 2827 2827 Size: 5410 Bme1580I G_kGCm'C Cuts at: 1790 2330 2445 3471 4627 1790 Size: 540 115 1026 1156 2573 Fragments arranged by size: 2573 1156 1026 540 115 BmgBI CAC'GTC Cuts at: 1318 2662 1318 Size: 1344 4066 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3141 4099 442 Size: 416 53 83 399 24 1724 958 1753 Fragments arranged by size: 1753 1724 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5410 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2606 4070 1239 Size: 1367 1464 2579 Fragments arranged by size: 2579 1464 1367 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3535 4400 4698 3535 Size: 865 298 4247 Fragments arranged by size: 4247 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4073 882 Size: 617 2574 2219 Fragments arranged by size: 2574 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2307 3201 493 Size: 711 1103 894 2702 Fragments arranged by size: 2702 1103 894 711 BsaBI GATnn'nnATC Cuts at: 5404 5404 Size: 5410 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2028 3217 3599 4234 Size: 53 83 186 936 495 1189 382 635 Cuts at: 4234 275 Size: 1451 Fragments arranged by size: 1451 1189 936 635 495 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3843 4241 4515 4662 5073 3843 Size: 398 274 147 411 4180 Fragments arranged by size: 4180 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5185 Fragments arranged by size: 5185 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2024 2027 1186 Size: 838 3 4569 Fragments arranged by size: 4569 838 3 BseYI C'CCAG_C Cuts at: 2323 4613 5109 2323 Size: 2290 496 2624 Fragments arranged by size: 2624 2290 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4837 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2190 2860 3621 4649 1540 Size: 275 186 189 670 761 1028 2301 Fragments arranged by size: 2301 1028 761 670 275 189 186 BsiHKAI G_wGCw'C Cuts at: 2622 3471 3556 4627 2622 Size: 849 85 1071 3405 Fragments arranged by size: 3405 1071 849 85 BsmI GAATG_Cn' Cuts at: 2413 2921 3014 2413 Size: 508 93 4809 Fragments arranged by size: 4809 508 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2009 2009 Size: 5410 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2330 2445 2455 2604 2622 2729 3471 Size: 399 540 115 10 149 18 107 742 Cuts at: 3471 3556 4627 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 540 399 149 115 107 85 18 10 BspEI T'CCGG_A Cuts at: 5073 5073 Size: 5410 BspHI T'CATG_A Cuts at: 3299 5029 3299 Size: 1730 3680 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2121 2356 2426 2765 844 Size: 1277 235 70 339 3489 Fragments arranged by size: 3489 1277 339 235 70 BsrBI CCG'CTC Cuts at: 1394 1823 2290 3297 1394 Size: 429 467 1007 3507 Fragments arranged by size: 3507 1007 467 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3905 4079 65 Size: 1183 2657 174 1396 Fragments arranged by size: 2657 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2185 2381 4054 4241 1700 Size: 110 375 196 1673 187 2869 Fragments arranged by size: 2869 1673 375 196 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5410 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5410 BssSI C'ACGA_G Cuts at: 2498 3470 4482 2498 Size: 972 1012 3426 Fragments arranged by size: 3426 1012 972 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5410 BstBI TT'CG_AA Cuts at: 4214 4214 Size: 5410 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5410 BstXI CCAn_nnnn'nTGG Cuts at: 2356 2356 Size: 5410 BstYI r'GATC_y Cuts at: 1972 2506 2740 3107 3492 3509 4961 5047 5059 Size: 534 234 367 385 17 1452 86 12 Cuts at: 5059 5405 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 534 385 367 346 234 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2243 2243 Size: 5410 BtgI C'CryG_G Cuts at: 513 1065 2004 2355 513 Size: 552 939 351 3568 Fragments arranged by size: 3568 939 552 351 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2391 487 Size: 683 56 1165 3506 Fragments arranged by size: 3506 1165 683 56 BtsI GCAGTG_nn' Cuts at: 2633 2926 3720 3740 2633 Size: 293 794 20 4303 Fragments arranged by size: 4303 794 293 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5410 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5375 DraI TTT'AAA Cuts at: 2714 2714 Size: 5410 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2162 4417 809 Size: 507 846 2255 1802 Fragments arranged by size: 2255 1802 846 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2123 2187 2857 Size: 54 1425 50 275 18 293 64 670 Cuts at: 2857 3746 5375 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 670 293 275 64 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2187 2857 1537 Size: 275 375 670 4090 Fragments arranged by size: 4090 670 375 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2273 2462 3340 1353 Size: 920 189 878 3423 Fragments arranged by size: 3423 920 878 189 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3997 4371 4517 387 Size: 1064 138 2408 374 146 1280 Fragments arranged by size: 2408 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2620 2620 Size: 5410 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2606 3473 4070 4857 5125 1239 Size: 393 974 867 597 787 268 1524 Fragments arranged by size: 1524 974 867 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2072 2493 2072 Size: 421 4989 EcoRI G'AATT_C Cuts at: 2719 3097 2719 Size: 378 5032 FseI GG_CCGG'CC Cuts at: 2189 2189 Size: 5410 FspI TGC'GCA Cuts at: 3916 3916 Size: 5410 HaeII r_GCGC'y Cuts at: 1678 4557 1678 Size: 2879 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2369 2401 2498 2530 4051 Size: 32 135 32 797 32 97 32 1521 Cuts at: 4051 4083 4125 4157 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1521 797 135 97 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2754 3000 669 Size: 988 281 816 246 3079 Fragments arranged by size: 3079 988 816 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5410 HpaI GTT'AAC Cuts at: 3000 3000 Size: 5410 KpnI G_GTAC'C Cuts at: 2735 2735 Size: 5410 MfeI C'AATT_G Cuts at: 3009 3009 Size: 5410 MluI A'CGCG_T Cuts at: 5286 5286 Size: 5410 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4524 4708 1305 Size: 3219 184 2007 Fragments arranged by size: 3219 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3588 Fragments arranged by size: 3588 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2354 2357 3368 3727 3886 518 Size: 648 1188 3 1011 359 159 2042 Fragments arranged by size: 2042 1188 1011 648 359 159 3 MspA1I CmG'CkG Cuts at: 1606 2826 3503 4651 4896 1606 Size: 1220 677 1148 245 2120 Fragments arranged by size: 2120 1220 1148 677 245 NaeI GCC'GGC Cuts at: 2187 2383 2187 Size: 196 5214 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3919 Fragments arranged by size: 3919 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5410 NgoMIV G'CCGG_C Cuts at: 2185 2381 2185 Size: 196 5214 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5410 NotI GC'GGCC_GC Cuts at: 2857 2857 Size: 5410 NspI r_CATG'y Cuts at: 1132 1229 2768 4313 1132 Size: 97 1539 1545 2229 Fragments arranged by size: 2229 1545 1539 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5410 PciI A'CATG_T Cuts at: 4309 4309 Size: 5410 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5410 PmeI GTTT'AAAC Cuts at: 2714 2714 Size: 5410 PmlI CAC'GTG Cuts at: 2307 2307 Size: 5410 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3479 3511 4992 5024 3479 Size: 32 1481 32 3865 Fragments arranged by size: 3865 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2072 2493 2072 Size: 421 4989 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5410 PsiI TTA'TAA Cuts at: 2980 2980 Size: 5410 PstI C_TGCA'G Cuts at: 830 2084 2367 2691 2762 830 Size: 1254 283 324 71 3478 Fragments arranged by size: 3478 1254 324 283 71 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5410 SacI G_AGCT'C Cuts at: 2622 2622 Size: 5410 SalI G'TCGA_C Cuts at: 1936 2752 1936 Size: 816 4594 SanDI GG'GwC_CC Cuts at: 2493 2493 Size: 5410 SapI GCTCTTCn'nnn_ Cuts at: 2273 2273 Size: 5410 SbfI CC_TGCA'GG Cuts at: 2762 2762 Size: 5410 ScaI AGT'ACT Cuts at: 3658 5370 3658 Size: 1712 3698 SfcI C'TryA_G Cuts at: 826 1045 2080 2363 2687 2758 3893 4574 4765 Size: 219 1035 283 324 71 1135 681 191 Cuts at: 4765 826 Size: 1471 Fragments arranged by size: 1471 1135 1035 681 324 283 219 191 71 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5410 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5410 SmaI CCC'GGG Cuts at: 1872 2537 2737 1872 Size: 665 200 4545 Fragments arranged by size: 4545 665 200 SmlI C'TyrA_G Cuts at: 820 1017 1943 3514 4415 4677 820 Size: 197 926 1571 901 262 1553 Fragments arranged by size: 1571 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3201 493 Size: 2708 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5410 SphI G_CATG'C Cuts at: 1132 2768 1132 Size: 1636 3774 SrfI GCCC'GGGC Cuts at: 2537 2537 Size: 5410 SspI AAT'ATT Cuts at: 5 52 3334 5 Size: 47 3282 2081 Fragments arranged by size: 3282 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5410 StyI C'CwwG_G Cuts at: 513 1065 2004 2595 2849 513 Size: 552 939 591 254 3074 Fragments arranged by size: 3074 939 591 552 254 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3437 3454 3607 3792 4237 325 Size: 1255 1857 17 153 185 445 1498 Fragments arranged by size: 1857 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3656 5368 96 Size: 274 80 33 51 1443 1679 1712 138 Fragments arranged by size: 1712 1679 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5370 5402 5370 Size: 32 5378 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5410 XbaI T'CTAG_A Cuts at: 2746 2746 Size: 5410 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5410 XmaI C'CCGG_G Cuts at: 1870 2535 2735 1870 Size: 665 200 4545 Fragments arranged by size: 4545 665 200 XmnI GAAnn'nnTTC Cuts at: 3539 3539 Size: 5410 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3218 276 Size: 53 83 186 936 1684 2468 Fragments arranged by size: 2468 1684 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I DraIII EcoNI EcoRV FalI FspAI KasI NarI NruI NsiI PacI PfoI PspOMI PspXI PsrI PvuII RsrII SacII SexAI SfiI SfoI SwaI XcmI