Restriction map for
pFN21AB8246
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# NO CUT ENZYMES ( 146 enzymes) #
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AatII AccI AccIII AclI AcyI
AflII AgeI AhdI AlwI AocI
Aor51HI ApaI AscI AseI AsnI
AspEI AspI AvaI AviII AvrII
BalI BamHI BbeI BbrPI BbvCI
BciVI BclI BcnI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BsaBI BsaHI BsaWI BseAI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstYI BstZ17I Bsu36I CelII
ClaI DraI DraII DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII Hin1I HincII HindII HindIII
HpaI KasI KspI MamI MflI
MluNI MroI MscI MseI MspA1I
NarI NciI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuII RcaI
SacII SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI Tru9I Tth111I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 60 enzymes) #
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Acc65I 213 GGTACC G^GTACC
AccII 150 CGCG CG^CG
AcsI 380 AAATTC [GA]^AATT[CT]
AfaI 214 GTAC GT^AC
AflIII 149 ACGCGT A^C[GA][CT]GT
Alw44I 145 GTGCAC G^TGCAC
AlwNI 235 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 145 GTGCAC G^TGCAC
ApoI 380 AAATTC [GA]^AATT[CT]
Asp700I 376 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 213 GGTACC G^GTACC
BanI 213 GGTACC G^G[CT][GA]CC
BanII 121 GAGCTC G[GA]GC[CT]^C
BcgI 243 GCAACACATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 337 TACGTG [CT]AC^GT[GA]
BsaI 205 GGTCTC GGTCTC(1/5)
BseRI 292 GAGGAG GAGGAG(10/8)
BsgI 274 GTGCAG GTGCAG(16/14)
BsiEI 80 CGATCG CG[GA][CT]^CG
BspMI 325 ACCTGC ACCTGC(4/8)
BsrBI 295 GAGCGG CCGCTC(-3/-3)
BsrFI 160 GCCGGC [GA]^CCGG[CT]
BsrI 602 ACTGG ACTGG(1/-1)
BstUI 150 CGCG CG^CG
BstXI 134 CCATCCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 180 GCGC GCG^C
Cfr10I 160 GCCGGC [GA]^CCGG[CT]
CpoI 5 CGGTCCG CG^G[AT]CCG
DsaI 140 CCGTGG C^C[GA][CT]GG
EaeI 425 TGGCCG [CT]^GGCC[GA]
EarI 524 CTCTTC CTCTTC(1/4)
HapII 161 CCGG C^CGG
HgaI 151 GCGTC GACGC(5/10)
HhaI 180 GCGC GCG^C
HinP1I 180 GCGC G^CGC
HinfI 712 GATTC G^A[ACGT]TC
HpaII 161 CCGG C^CGG
KpnI 213 GGTACC GGTAC^C
Ksp632I 524 CTCTTC CTCTTC(1/4)
MaeII 338 ACGT A^CGT
MaeIII 319 GTCAC ^GT[ACGT]AC
MfeI 497 CAATTG C^AATTG
MluI 149 ACGCGT A^CGCGT
MspI 161 CCGG C^CGG
MunI 497 CAATTG C^AATTG
MvnI 150 CGCG CG^CG
NaeI 160 GCCGGC GCC^GGC
NgoMIV 160 GCCGGC G^CCGGC
NlaIV 213 GGTACC GG[ACGT]^[ACGT]CC
PvuI 80 CGATCG CGAT^CG
RsaI 214 GTAC GT^AC
RsrII 5 CGGTCCG CG^G[AT]CCG
SacI 121 GAGCTC GAGCT^C
SexAI 431 ACCAGGT A^CC[AT]GGT
SfcI 102 CTACAG C^T[GA][CT]AG
SmlI 397 CTCAAG C^T[CT][GA]AG
SphI 704 GCATGC GCATG^C
TfiI 712 GATTC G^A[AT]TC
Tsp45I 319 GTCAC ^GT[GC]AC
XmnI 376 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
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AvaII 6 GGTCC G^G[AT]CC
348 GGTCC
BfaI 439 CTAG C^TAG
686 CTAG
BsmAI 370 GAGAC GTCTC(1/5)
206 GTCTC
BsmFI 550 GGGAC GGGAC(10/14)
535 GTCCC
Cfr13I 6 GGTCC G^G[ACGT]CC
348 GGTCC
DdeI 90 CTCAG C^T[ACGT]AG
303 CTCAG
DpnII 36 GATC ^GATC
81 GATC
EcoT14I 308 CCTTGG C^C[AT][AT]GG
344 CCTTGG
MaeI 439 CTAG C^TAG
686 CTAG
MboI 36 GATC ^GATC
81 GATC
NdeII 36 GATC ^GATC
81 GATC
NlaIII 183 CATG CATG^
705 CATG
NspI 182 GCATGT [GA]CATG^[CT]
704 GCATGC
Sau3AI 36 GATC ^GATC
81 GATC
Sau96I 6 GGTCC G^G[ACGT]CC
348 GGTCC
SfaNI 170 GCATC GCATC(5/9)
483 GCATC
StyI 308 CCTTGG C^C[AT][AT]GG
344 CCTTGG
TaqI 20 TCGA T^CGA
113 TCGA
Tsp509I 381 AATT ^AATT
498 AATT
TthHB8I 20 TCGA T^CGA
113 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AciI AluI AspHI BbsI BbvI
BmyI BpuAI BsaJI BsiHKAI BsiYI
BslI Bsp1286I BsrDI BssKI BstF5I
BstNI Cac8I EcoRII FauI Fnu4HI
FokI HaeIII HphI ItaI MboII
MnlI MslI MvaI MwoI ScrFI
TseI TspRI