(Circular) (Six-base) MAPSORT of: pFN21AB8228.seq Check: 6993 from: 1 to: 5932 pFN21AB8228 5932 bp With 182 enzymes: SgfI * February 27, 2009 14:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3742 278 Size: 53 83 186 936 2206 2468 Fragments arranged by size: 2468 2206 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3275 1937 Size: 1338 4594 Acc65I G'GTAC_C Cuts at: 3253 3253 Size: 5932 AclI AA'CG_TT Cuts at: 1563 4059 4432 1563 Size: 2496 373 3063 Fragments arranged by size: 3063 2496 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3995 5379 5647 1632 Size: 2363 1384 268 1917 Fragments arranged by size: 2363 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5735 AflIII A'CryG_T Cuts at: 1203 4831 5808 1203 Size: 3628 977 1327 Fragments arranged by size: 3628 1327 977 AgeI A'CCGG_T Cuts at: 4763 4763 Size: 5932 AhdI GACnn_n'nnGTC Cuts at: 4661 4661 Size: 5932 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5932 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5932 AlwNI CAG_nnn'CTG Cuts at: 2935 2989 3058 3675 5247 2935 Size: 54 69 617 1572 3620 Fragments arranged by size: 3620 1572 617 69 54 ApaLI G'TGCA_C Cuts at: 3989 5145 3989 Size: 1156 4776 ApoI r'AATT_y Cuts at: 1493 2093 3241 3467 3619 5728 1493 Size: 600 1148 226 152 2109 1697 Fragments arranged by size: 2109 1697 1148 600 226 152 AseI AT'TA_AT Cuts at: 160 1032 4486 160 Size: 872 3454 1606 Fragments arranged by size: 3454 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5932 AvaI C'yCGr_G Cuts at: 1870 1943 2069 2400 2466 2908 3257 1870 Size: 73 126 331 66 442 349 4545 Fragments arranged by size: 4545 442 349 331 126 73 66 BamHI G'GATC_C Cuts at: 2925 3262 3629 2925 Size: 337 367 5228 Fragments arranged by size: 5228 367 337 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2323 2773 3253 4708 618 Size: 325 217 627 536 450 480 1455 1842 Fragments arranged by size: 1842 1455 627 536 480 450 325 217 BanII G_rGCy'C Cuts at: 1391 2247 3251 1391 Size: 856 1004 4072 Fragments arranged by size: 4072 1004 856 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2589 928 Size: 971 690 4271 Fragments arranged by size: 4271 971 690 BbvCI CC'TCA_GC Cuts at: 2877 2877 Size: 5932 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4123 4157 1903 Size: 34 2186 34 3678 Fragments arranged by size: 3678 2186 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3824 5034 3824 Size: 1210 4722 BclI T'GATC_A Cuts at: 1548 1548 Size: 5932 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2657 4543 136 Size: 107 122 71 631 605 985 1886 1525 Fragments arranged by size: 1886 1525 985 631 605 122 107 71 BglII A'GATC_T Cuts at: 5927 5927 Size: 5932 BlpI GC'TnA_GC Cuts at: 3349 3349 Size: 5932 Bme1580I G_kGCm'C Cuts at: 1790 3993 5149 1790 Size: 2203 1156 2573 Fragments arranged by size: 2573 2203 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5932 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2999 3663 4621 Size: 416 53 83 399 24 1582 664 958 Cuts at: 4621 442 Size: 1753 Fragments arranged by size: 1753 1582 958 664 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5932 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2840 2849 2972 3083 4592 1239 Size: 1601 9 123 111 1509 2579 Fragments arranged by size: 2579 1601 1509 123 111 9 Bpu10I CC'TnA_GC Cuts at: 2877 2877 Size: 5932 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4057 4922 5220 4057 Size: 865 298 4769 Fragments arranged by size: 4769 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3033 3138 4595 882 Size: 617 1534 105 1457 2219 Fragments arranged by size: 2219 1534 1457 617 105 BsaAI yAC'GTr Cuts at: 493 1204 3723 493 Size: 711 2519 2702 Fragments arranged by size: 2702 2519 711 BsaBI GATnn'nnATC Cuts at: 5926 5926 Size: 5932 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2789 3739 4121 4756 Size: 53 83 186 936 1256 950 382 635 Cuts at: 4756 275 Size: 1451 Fragments arranged by size: 1451 1256 950 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2088 2247 3216 4365 4763 5037 5184 5595 2088 Size: 159 969 1149 398 274 147 411 2425 Fragments arranged by size: 2425 1149 969 411 398 274 159 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5707 Fragments arranged by size: 5707 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2453 2837 2969 1186 Size: 1267 384 132 4149 Fragments arranged by size: 4149 1267 384 132 BseYI C'CCAG_C Cuts at: 2082 2652 3079 5135 5631 2082 Size: 570 427 2056 496 2383 Fragments arranged by size: 2383 2056 570 496 427 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2046 1163 Size: 573 310 5049 Fragments arranged by size: 5049 573 310 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3382 4143 5171 1540 Size: 275 186 1381 761 1028 2301 Fragments arranged by size: 2301 1381 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2247 3993 4078 5149 2247 Size: 1746 85 1071 3030 Fragments arranged by size: 3030 1746 1071 85 BsmI GAATG_Cn' Cuts at: 3443 3536 3443 Size: 93 5839 BsmBI CGTCTCn'nnnn_ Cuts at: 3059 3059 Size: 5932 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2247 3251 3993 4078 5149 1391 Size: 399 457 1004 742 85 1071 2174 Fragments arranged by size: 2174 1071 1004 742 457 399 85 BspEI T'CCGG_A Cuts at: 2088 2247 5595 2088 Size: 159 3348 2425 Fragments arranged by size: 3348 2425 159 BspHI T'CATG_A Cuts at: 3821 5551 3821 Size: 1730 4202 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3174 3287 844 Size: 2330 113 3489 Fragments arranged by size: 3489 2330 113 BsrBI CCG'CTC Cuts at: 1394 1823 2678 3819 1394 Size: 429 855 1141 3507 Fragments arranged by size: 3507 1141 855 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4427 4601 65 Size: 1183 3179 174 1396 Fragments arranged by size: 3179 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2667 4576 4763 1700 Size: 110 857 1909 187 2869 Fragments arranged by size: 2869 1909 857 187 110 BsrGI T'GTAC_A Cuts at: 96 3150 96 Size: 3054 2878 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5932 BssSI C'ACGA_G Cuts at: 3992 5004 3992 Size: 1012 4920 BstAPI GCAn_nnn'nTGC Cuts at: 794 3058 794 Size: 2264 3668 BstBI TT'CG_AA Cuts at: 4736 4736 Size: 5932 BstEII G'GTnAC_C Cuts at: 1184 2428 1184 Size: 1244 4688 BstYI r'GATC_y Cuts at: 1972 2826 2925 3262 3629 4014 4031 5483 5569 Size: 854 99 337 367 385 17 1452 86 Cuts at: 5569 5581 5927 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 854 385 367 346 337 99 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2040 2595 2040 Size: 555 5377 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5380 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5193 Fragments arranged by size: 5193 683 56 BtsI GCAGTG_nn' Cuts at: 2352 3448 4242 4262 2352 Size: 1096 794 20 4022 Fragments arranged by size: 4022 1096 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5932 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5897 DraI TTT'AAA Cuts at: 3236 3236 Size: 5932 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4939 809 Size: 507 3623 1802 Fragments arranged by size: 3623 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3379 4268 5897 Size: 54 1425 50 275 18 1549 889 1629 Cuts at: 5897 8 Size: 43 Fragments arranged by size: 1629 1549 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3379 1537 Size: 275 1567 4090 Fragments arranged by size: 4090 1567 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2235 2352 3215 3862 1353 Size: 882 117 863 647 3423 Fragments arranged by size: 3423 882 863 647 117 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2014 2686 3021 4519 4893 5039 Size: 1064 138 425 672 335 1498 374 146 Cuts at: 5039 387 Size: 1280 Fragments arranged by size: 1498 1280 1064 672 425 374 335 146 138 EcoICRI GAG'CTC Cuts at: 2245 2245 Size: 5932 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2840 2849 2972 3083 3995 4592 5379 Size: 393 1208 9 123 111 912 597 787 Cuts at: 5379 5647 1239 Size: 268 1524 Fragments arranged by size: 1524 1208 912 787 597 393 268 123 111 9 EcoO109I rG'GnC_Cy Cuts at: 2036 2932 3016 2036 Size: 896 84 4952 Fragments arranged by size: 4952 896 84 EcoRI G'AATT_C Cuts at: 3241 3619 3241 Size: 378 5554 FspI TGC'GCA Cuts at: 4438 4438 Size: 5932 HaeII r_GCGC'y Cuts at: 1678 5079 1678 Size: 3401 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2351 2383 2816 2818 2834 Size: 32 135 32 779 32 433 2 16 Cuts at: 2834 2848 2850 2866 3029 3061 3077 3109 4573 Size: 14 2 16 163 32 16 32 1464 Cuts at: 4573 4605 4647 4679 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1464 779 433 163 135 42 32 32 32 32 32 32 32 16 16 16 14 2 2 HincII GTy'rAC Cuts at: 669 1657 1938 2632 2787 3276 3522 669 Size: 988 281 694 155 489 246 3079 Fragments arranged by size: 3079 988 694 489 281 246 155 HindIII A'AGCT_T Cuts at: 748 748 Size: 5932 HpaI GTT'AAC Cuts at: 3522 3522 Size: 5932 KpnI G_GTAC'C Cuts at: 3257 3257 Size: 5932 MfeI C'AATT_G Cuts at: 3531 3531 Size: 5932 MluI A'CGCG_T Cuts at: 5808 5808 Size: 5932 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5046 5230 1305 Size: 3741 184 2007 Fragments arranged by size: 3741 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4110 Fragments arranged by size: 4110 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3890 4249 4408 518 Size: 648 2724 359 159 2042 Fragments arranged by size: 2724 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2656 2808 2839 2869 2917 3055 3348 4025 Size: 1050 152 31 30 48 138 293 677 Cuts at: 4025 5173 5418 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 1050 677 293 245 152 138 48 31 30 NaeI GCC'GGC Cuts at: 2669 2669 Size: 5932 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5380 NdeI CA'TA_TG Cuts at: 387 387 Size: 5932 NgoMIV G'CCGG_C Cuts at: 2667 2667 Size: 5932 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5932 NotI GC'GGCC_GC Cuts at: 3379 3379 Size: 5932 NspI r_CATG'y Cuts at: 1132 1229 3290 4835 1132 Size: 97 2061 1545 2229 Fragments arranged by size: 2229 2061 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5932 PciI A'CATG_T Cuts at: 4831 4831 Size: 5932 PflMI CCAn_nnn'nTGG Cuts at: 1108 2935 1108 Size: 1827 4105 PfoI T'CCnGG_A Cuts at: 2921 2928 2921 Size: 7 5925 PmeI GTTT'AAAC Cuts at: 3236 3236 Size: 5932 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4001 4033 5514 5546 4001 Size: 32 1481 32 4387 Fragments arranged by size: 4387 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2036 2932 3016 2036 Size: 896 84 4952 Fragments arranged by size: 4952 896 84 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5932 PsiI TTA'TAA Cuts at: 3502 3502 Size: 5932 PstI C_TGCA'G Cuts at: 830 3126 3171 3284 830 Size: 2296 45 113 3478 Fragments arranged by size: 3478 2296 113 45 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5932 PvuII CAG'CTG Cuts at: 2656 2869 2917 3055 2656 Size: 213 48 138 5533 Fragments arranged by size: 5533 213 138 48 SacI G_AGCT'C Cuts at: 2247 2247 Size: 5932 SalI G'TCGA_C Cuts at: 1936 3274 1936 Size: 1338 4594 SapI GCTCTTCn'nnn_ Cuts at: 2235 3215 2235 Size: 980 4952 SbfI CC_TGCA'GG Cuts at: 3284 3284 Size: 5932 ScaI AGT'ACT Cuts at: 4180 5892 4180 Size: 1712 4220 SexAI A'CCwGG_T Cuts at: 2934 2934 Size: 5932 SfcI C'TryA_G Cuts at: 826 1045 3122 3167 3280 4415 5096 5287 826 Size: 219 2077 45 113 1135 681 191 1471 Fragments arranged by size: 2077 1471 1135 681 219 191 113 45 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5932 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5932 SmaI CCC'GGG Cuts at: 1872 2910 3259 1872 Size: 1038 349 4545 Fragments arranged by size: 4545 1038 349 SmlI C'TyrA_G Cuts at: 820 1017 1943 2466 4036 4937 5199 820 Size: 197 926 523 1570 901 262 1553 Fragments arranged by size: 1570 1553 926 901 523 262 197 SnaBI TAC'GTA Cuts at: 493 3723 493 Size: 3230 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5932 SphI G_CATG'C Cuts at: 1132 3290 1132 Size: 2158 3774 SspI AAT'ATT Cuts at: 5 52 2200 3856 5 Size: 47 2148 1656 2081 Fragments arranged by size: 2148 2081 1656 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5932 StyI C'CwwG_G Cuts at: 513 1065 2562 2732 3012 3371 513 Size: 552 1497 170 280 359 3074 Fragments arranged by size: 3074 1497 552 359 280 170 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2341 2791 3015 3959 3976 4129 4314 Size: 1255 761 450 224 944 17 153 185 Cuts at: 4314 4759 325 Size: 445 1498 Fragments arranged by size: 1498 1255 944 761 450 445 224 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3150 4178 5890 Size: 274 80 33 51 1443 1173 1028 1712 Cuts at: 5890 96 Size: 138 Fragments arranged by size: 1712 1443 1173 1028 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5892 5924 5892 Size: 32 5900 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5932 XbaI T'CTAG_A Cuts at: 3268 3268 Size: 5932 XhoI C'TCGA_G Cuts at: 1943 2466 1943 Size: 523 5409 XmaI C'CCGG_G Cuts at: 1870 2908 3257 1870 Size: 1038 349 4545 Fragments arranged by size: 4545 1038 349 XmnI GAAnn'nnTTC Cuts at: 4061 4061 Size: 5932 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3740 276 Size: 53 83 186 936 2206 2468 Fragments arranged by size: 2468 2206 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI XcmI