(Circular) (Six-base) MAPSORT of: pFN21AB8223.seq Check: 8387 from: 1 to: 5521 pFN21AB8223 5521 bp With 182 enzymes: SgfI * February 27, 2009 14:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2440 2440 Size: 5521 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3331 278 Size: 53 83 186 936 1795 2468 Fragments arranged by size: 2468 1795 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2864 1937 Size: 927 4594 Acc65I G'GTAC_C Cuts at: 2842 2842 Size: 5521 AclI AA'CG_TT Cuts at: 1563 3648 4021 1563 Size: 2085 373 3063 Fragments arranged by size: 3063 2085 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3584 4968 5236 1632 Size: 1952 1384 268 1917 Fragments arranged by size: 1952 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5324 AflIII A'CryG_T Cuts at: 1203 2505 4420 5397 1203 Size: 1302 1915 977 1327 Fragments arranged by size: 1915 1327 1302 977 AgeI A'CCGG_T Cuts at: 4352 4352 Size: 5521 AhdI GACnn_n'nnGTC Cuts at: 4250 4250 Size: 5521 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5521 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5521 AlwNI CAG_nnn'CTG Cuts at: 3264 4836 3264 Size: 1572 3949 ApaLI G'TGCA_C Cuts at: 3578 4734 3578 Size: 1156 4365 ApoI r'AATT_y Cuts at: 1493 2830 3056 3208 5317 1493 Size: 1337 226 152 2109 1697 Fragments arranged by size: 2109 1697 1337 226 152 AseI AT'TA_AT Cuts at: 160 1032 4075 160 Size: 872 3043 1606 Fragments arranged by size: 3043 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5521 AvaI C'yCGr_G Cuts at: 1870 1943 2846 1870 Size: 73 903 4545 Fragments arranged by size: 4545 903 73 BamHI G'GATC_C Cuts at: 2851 3218 2851 Size: 367 5154 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2842 4297 618 Size: 325 217 627 1055 1455 1842 Fragments arranged by size: 1842 1455 1055 627 325 217 BanII G_rGCy'C Cuts at: 1391 2493 2840 1391 Size: 1102 347 4072 Fragments arranged by size: 4072 1102 347 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4550 BbvCI CC'TCA_GC Cuts at: 2646 2646 Size: 5521 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3712 3746 1903 Size: 34 1775 34 3678 Fragments arranged by size: 3678 1775 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3413 4623 3413 Size: 1210 4311 BclI T'GATC_A Cuts at: 1548 1548 Size: 5521 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2791 4132 136 Size: 107 122 71 631 605 1119 1341 1525 Fragments arranged by size: 1525 1341 1119 631 605 122 107 71 BglII A'GATC_T Cuts at: 5516 5516 Size: 5521 BlpI GC'TnA_GC Cuts at: 2571 2613 2667 2938 2571 Size: 42 54 271 5154 Fragments arranged by size: 5154 271 54 42 Bme1580I G_kGCm'C Cuts at: 1790 2106 3582 4738 1790 Size: 316 1476 1156 2573 Fragments arranged by size: 2573 1476 1156 316 BmgBI CAC'GTC Cuts at: 1318 2506 1318 Size: 1188 4333 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3252 4210 442 Size: 416 53 83 399 24 1835 958 1753 Fragments arranged by size: 1835 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5521 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2458 2671 2458 Size: 213 5308 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2428 2433 2792 4181 1239 Size: 1189 5 359 1389 2579 Fragments arranged by size: 2579 1389 1189 359 5 Bpu10I CC'TnA_GC Cuts at: 2050 2646 2050 Size: 596 4925 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2369 3646 4511 4809 2369 Size: 1277 865 298 3081 Fragments arranged by size: 3081 1277 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2446 4184 882 Size: 617 947 1738 2219 Fragments arranged by size: 2219 1738 947 617 BsaAI yAC'GTr Cuts at: 493 1204 2225 3312 493 Size: 711 1021 1087 2702 Fragments arranged by size: 2702 1087 1021 711 BsaBI GATnn'nnATC Cuts at: 5515 5515 Size: 5521 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3328 3710 4345 275 Size: 53 83 186 936 1795 382 635 1451 Fragments arranged by size: 1795 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3954 4352 4626 4773 5184 3954 Size: 398 274 147 411 4291 Fragments arranged by size: 4291 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2055 2085 1178 Size: 30 165 30 652 30 4614 Fragments arranged by size: 4614 652 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2795 1186 Size: 1609 3912 BseYI C'CCAG_C Cuts at: 2099 4724 5220 2099 Size: 2625 496 2400 Fragments arranged by size: 2625 2400 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2159 1163 Size: 573 423 4525 Fragments arranged by size: 4525 573 423 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2971 3732 4760 1540 Size: 275 186 970 761 1028 2301 Fragments arranged by size: 2301 1028 970 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2805 3582 3667 4738 2805 Size: 777 85 1071 3588 Fragments arranged by size: 3588 1071 777 85 BsmI GAATG_Cn' Cuts at: 3032 3125 3032 Size: 93 5428 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2106 2493 2805 2840 3582 3667 4738 Size: 399 316 387 312 35 742 85 1071 Cuts at: 4738 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 387 316 312 85 35 BspEI T'CCGG_A Cuts at: 5184 5184 Size: 5521 BspHI T'CATG_A Cuts at: 3410 5140 3410 Size: 1730 3791 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2440 2790 2876 844 Size: 1596 350 86 3489 Fragments arranged by size: 3489 1596 350 86 BsrBI CCG'CTC Cuts at: 1394 1823 3408 1394 Size: 429 1585 3507 Fragments arranged by size: 3507 1585 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4016 4190 65 Size: 1183 2768 174 1396 Fragments arranged by size: 2768 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4165 4352 1700 Size: 110 2355 187 2869 Fragments arranged by size: 2869 2355 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5521 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5521 BssSI C'ACGA_G Cuts at: 3581 4593 3581 Size: 1012 4509 BstAPI GCAn_nnn'nTGC Cuts at: 794 2800 794 Size: 2006 3515 BstBI TT'CG_AA Cuts at: 4325 4325 Size: 5521 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5521 BstXI CCAn_nnnn'nTGG Cuts at: 2566 2599 2686 2566 Size: 33 87 5401 Fragments arranged by size: 5401 87 33 BstYI r'GATC_y Cuts at: 1972 2379 2851 3218 3603 3620 5072 5158 5170 Size: 407 472 367 385 17 1452 86 12 Cuts at: 5170 5516 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 472 407 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2131 2180 513 Size: 552 939 127 49 3854 Fragments arranged by size: 3854 939 552 127 49 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2728 487 Size: 683 56 1502 3280 Fragments arranged by size: 3280 1502 683 56 BtsI GCAGTG_nn' Cuts at: 2276 2691 3037 3831 3851 2276 Size: 415 346 794 20 3946 Fragments arranged by size: 3946 794 415 346 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5521 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5486 DraI TTT'AAA Cuts at: 2825 2825 Size: 5521 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4528 809 Size: 507 3212 1802 Fragments arranged by size: 3212 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2631 2688 2968 Size: 54 1425 50 275 18 801 57 280 Cuts at: 2968 3857 5486 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 801 280 275 57 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2968 1537 Size: 275 1156 4090 Fragments arranged by size: 4090 1156 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2294 3451 1353 Size: 941 1157 3423 Fragments arranged by size: 3423 1157 941 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4108 4482 4628 387 Size: 1064 138 2519 374 146 1280 Fragments arranged by size: 2519 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2428 2433 2792 3584 4181 4968 5236 Size: 393 796 5 359 792 597 787 268 Cuts at: 5236 1239 Size: 1524 Fragments arranged by size: 1524 796 792 787 597 393 359 268 5 EcoRI G'AATT_C Cuts at: 2830 3208 2830 Size: 378 5143 FspI TGC'GCA Cuts at: 2282 2720 4027 2282 Size: 438 1307 3776 Fragments arranged by size: 3776 1307 438 HaeII r_GCGC'y Cuts at: 1678 4668 1678 Size: 2990 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2100 2132 2406 2424 2438 Size: 32 135 32 528 32 274 18 14 Cuts at: 2438 2456 2492 2524 4162 4194 4236 4268 1373 Size: 18 36 32 1638 32 42 32 2626 Fragments arranged by size: 2626 1638 528 274 135 42 36 32 32 32 32 32 32 18 18 14 HincII GTy'rAC Cuts at: 669 1657 1938 2269 2865 3111 669 Size: 988 281 331 596 246 3079 Fragments arranged by size: 3079 988 596 331 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5521 HpaI GTT'AAC Cuts at: 3111 3111 Size: 5521 KpnI G_GTAC'C Cuts at: 2846 2846 Size: 5521 MfeI C'AATT_G Cuts at: 3120 3120 Size: 5521 MluI A'CGCG_T Cuts at: 5397 5397 Size: 5521 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4635 4819 1305 Size: 3330 184 2007 Fragments arranged by size: 3330 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2690 10 Size: 54 1425 343 858 2841 Fragments arranged by size: 2841 1425 858 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3479 3838 3997 518 Size: 648 2313 359 159 2042 Fragments arranged by size: 2313 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2666 2789 2812 2937 3614 4762 5007 1606 Size: 1060 123 23 125 677 1148 245 2120 Fragments arranged by size: 2120 1148 1060 677 245 125 123 23 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4030 Fragments arranged by size: 4030 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5521 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5521 NotI GC'GGCC_GC Cuts at: 2968 2968 Size: 5521 NspI r_CATG'y Cuts at: 1132 1229 2879 4424 1132 Size: 97 1650 1545 2229 Fragments arranged by size: 2229 1650 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5521 PciI A'CATG_T Cuts at: 4420 4420 Size: 5521 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5521 PfoI T'CCnGG_A Cuts at: 2496 2496 Size: 5521 PmeI GTTT'AAAC Cuts at: 2825 2825 Size: 5521 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3590 3622 5103 5135 3590 Size: 32 1481 32 3976 Fragments arranged by size: 3976 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5521 PsiI TTA'TAA Cuts at: 3091 3091 Size: 5521 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2762 2794 2762 Size: 32 5489 PstI C_TGCA'G Cuts at: 830 2698 2737 2873 830 Size: 1868 39 136 3478 Fragments arranged by size: 3478 1868 136 39 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5521 PvuII CAG'CTG Cuts at: 2666 2812 2666 Size: 146 5375 SalI G'TCGA_C Cuts at: 1936 2863 1936 Size: 927 4594 SbfI CC_TGCA'GG Cuts at: 2873 2873 Size: 5521 ScaI AGT'ACT Cuts at: 3769 5481 3769 Size: 1712 3809 SfcI C'TryA_G Cuts at: 826 1045 2694 2733 2869 4004 4685 4876 826 Size: 219 1649 39 136 1135 681 191 1471 Fragments arranged by size: 1649 1471 1135 681 219 191 136 39 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5521 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5521 SmaI CCC'GGG Cuts at: 1872 2848 1872 Size: 976 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2384 3625 4526 4788 820 Size: 197 926 441 1241 901 262 1553 Fragments arranged by size: 1553 1241 926 901 441 262 197 SnaBI TAC'GTA Cuts at: 493 3312 493 Size: 2819 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5521 SphI G_CATG'C Cuts at: 1132 2879 1132 Size: 1747 3774 SspI AAT'ATT Cuts at: 5 52 3445 5 Size: 47 3393 2081 Fragments arranged by size: 3393 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5521 StyI C'CwwG_G Cuts at: 513 1065 2004 2658 2960 513 Size: 552 939 654 302 3074 Fragments arranged by size: 3074 939 654 552 302 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2139 2441 3548 3565 3718 3903 4348 Size: 1255 559 302 1107 17 153 185 445 Cuts at: 4348 325 Size: 1498 Fragments arranged by size: 1498 1255 1107 559 445 302 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3767 5479 96 Size: 274 80 33 51 1443 1790 1712 138 Fragments arranged by size: 1790 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2055 2087 2268 2300 5481 5513 2055 Size: 32 181 32 3181 32 2063 Fragments arranged by size: 3181 2063 181 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5521 XbaI T'CTAG_A Cuts at: 2857 2857 Size: 5521 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2599 2599 Size: 5521 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5521 XmaI C'CCGG_G Cuts at: 1870 2846 1870 Size: 976 4545 XmnI GAAnn'nnTTC Cuts at: 2537 3650 2537 Size: 1113 4408 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3329 276 Size: 53 83 186 936 1795 2468 Fragments arranged by size: 2468 1795 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI