(Circular) (Six-base) MAPSORT of: pFN21AB8221.seq Check: 2136 from: 1 to: 5449 pFN21AB8221 5449 bp With 182 enzymes: SgfI * February 27, 2009 14:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3259 278 Size: 53 83 186 936 1723 2468 Fragments arranged by size: 2468 1723 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2792 1937 Size: 855 4594 Acc65I G'GTAC_C Cuts at: 2770 2770 Size: 5449 AclI AA'CG_TT Cuts at: 1563 3576 3949 1563 Size: 2013 373 3063 Fragments arranged by size: 3063 2013 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3512 4896 5164 1632 Size: 1880 1384 268 1917 Fragments arranged by size: 1917 1880 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5252 AflIII A'CryG_T Cuts at: 1203 4348 5325 1203 Size: 3145 977 1327 Fragments arranged by size: 3145 1327 977 AgeI A'CCGG_T Cuts at: 4280 4280 Size: 5449 AhdI GACnn_n'nnGTC Cuts at: 2617 4178 2617 Size: 1561 3888 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5449 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5449 AlwNI CAG_nnn'CTG Cuts at: 2324 2630 3192 4764 2324 Size: 306 562 1572 3009 Fragments arranged by size: 3009 1572 562 306 ApaI G_GGCC'C Cuts at: 2190 2190 Size: 5449 ApaLI G'TGCA_C Cuts at: 2744 3506 4662 2744 Size: 762 1156 3531 Fragments arranged by size: 3531 1156 762 ApoI r'AATT_y Cuts at: 1493 2758 2984 3136 5245 1493 Size: 1265 226 152 2109 1697 Fragments arranged by size: 2109 1697 1265 226 152 AseI AT'TA_AT Cuts at: 160 1032 4003 160 Size: 872 2971 1606 Fragments arranged by size: 2971 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5449 AvaI C'yCGr_G Cuts at: 1870 1943 2183 2774 1870 Size: 73 240 591 4545 Fragments arranged by size: 4545 591 240 73 BamHI G'GATC_C Cuts at: 2017 2779 3146 2017 Size: 762 367 4320 Fragments arranged by size: 4320 762 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2027 2039 2078 2177 2770 Size: 325 217 627 240 12 39 99 593 Cuts at: 2770 4225 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 593 325 240 217 99 39 12 BanII G_rGCy'C Cuts at: 1391 2190 2768 1391 Size: 799 578 4072 Fragments arranged by size: 4072 799 578 BbeI G_GCGC'C Cuts at: 2031 2043 2082 2181 2031 Size: 12 39 99 5299 Fragments arranged by size: 5299 99 39 12 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2097 928 Size: 971 198 4280 Fragments arranged by size: 4280 971 198 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3640 3674 1903 Size: 34 1703 34 3678 Fragments arranged by size: 3678 1703 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2342 3341 4551 2342 Size: 999 1210 3240 Fragments arranged by size: 3240 1210 999 BclI T'GATC_A Cuts at: 1548 1548 Size: 5449 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2111 4060 136 Size: 107 122 71 631 605 439 1949 1525 Fragments arranged by size: 1949 1525 631 605 439 122 107 71 BglII A'GATC_T Cuts at: 5444 5444 Size: 5449 BlpI GC'TnA_GC Cuts at: 2010 2866 2010 Size: 856 4593 Bme1580I G_kGCm'C Cuts at: 1790 2190 2748 3510 4666 1790 Size: 400 558 762 1156 2573 Fragments arranged by size: 2573 1156 762 558 400 BmgBI CAC'GTC Cuts at: 1318 2530 1318 Size: 1212 4237 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3180 4138 442 Size: 416 53 83 399 24 1763 958 1753 Fragments arranged by size: 1763 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5449 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4109 1239 Size: 2870 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2711 3574 4439 4737 2711 Size: 863 865 298 3423 Fragments arranged by size: 3423 865 863 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4112 882 Size: 617 2613 2219 Fragments arranged by size: 2613 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3240 493 Size: 711 2036 2702 Fragments arranged by size: 2702 2036 711 BsaBI GATnn'nnATC Cuts at: 5443 5443 Size: 5449 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2028 2040 2079 2178 Size: 53 83 186 936 495 12 39 99 Cuts at: 2178 3256 3638 4273 275 Size: 1078 382 635 1451 Fragments arranged by size: 1451 1078 936 635 495 382 186 99 83 53 39 12 BsaWI w'CCGG_w Cuts at: 2218 3882 4280 4554 4701 5112 2218 Size: 1664 398 274 147 411 2555 Fragments arranged by size: 2555 1664 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5224 Fragments arranged by size: 5224 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5449 BseYI C'CCAG_C Cuts at: 2059 2190 4652 5148 2059 Size: 131 2462 496 2360 Fragments arranged by size: 2462 2360 496 131 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4876 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2899 3660 4688 1540 Size: 275 186 898 761 1028 2301 Fragments arranged by size: 2301 1028 898 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2748 3510 3595 4666 2748 Size: 762 85 1071 3531 Fragments arranged by size: 3531 1071 762 85 BsmI GAATG_Cn' Cuts at: 2960 3053 2960 Size: 93 5356 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2190 2748 2768 3510 3595 4666 1391 Size: 399 400 558 20 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 558 400 399 85 20 BspEI T'CCGG_A Cuts at: 2218 5112 2218 Size: 2894 2555 BspHI T'CATG_A Cuts at: 3338 5068 3338 Size: 1730 3719 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2804 844 Size: 1960 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3336 1394 Size: 429 1513 3507 Fragments arranged by size: 3507 1513 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3944 4118 65 Size: 1183 2696 174 1396 Fragments arranged by size: 2696 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2036 2075 2081 4093 4280 1700 Size: 110 226 39 6 2012 187 2869 Fragments arranged by size: 2869 2012 226 187 110 39 6 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5449 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5449 BssSI C'ACGA_G Cuts at: 3509 4521 3509 Size: 1012 4437 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5449 BstBI TT'CG_AA Cuts at: 4253 4253 Size: 5449 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5449 BstYI r'GATC_y Cuts at: 1972 2017 2779 3146 3531 3548 5000 5086 5098 Size: 45 762 367 385 17 1452 86 12 Cuts at: 5098 5444 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 762 385 367 346 86 45 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3958 Fragments arranged by size: 3958 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4710 Fragments arranged by size: 4710 683 56 BtsI GCAGTG_nn' Cuts at: 2965 3759 3779 2965 Size: 794 20 4635 Fragments arranged by size: 4635 794 20 ClaI AT'CG_AT Cuts at: 1554 2308 1554 Size: 754 4695 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5414 DraI TTT'AAA Cuts at: 2753 2753 Size: 5449 DraIII CAC_nnn'GTG Cuts at: 2634 2634 Size: 5449 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4456 809 Size: 507 3140 1802 Fragments arranged by size: 3140 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2073 2896 3785 Size: 54 1425 50 275 18 243 823 889 Cuts at: 3785 5414 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 823 275 243 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2896 1537 Size: 275 1084 4090 Fragments arranged by size: 4090 1084 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3379 1353 Size: 2026 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4036 4410 4556 387 Size: 1064 138 2447 374 146 1280 Fragments arranged by size: 2447 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3512 4109 4896 5164 1239 Size: 393 1880 597 787 268 1524 Fragments arranged by size: 1880 1524 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2576 2729 2576 Size: 153 5296 EcoO109I rG'GnC_Cy Cuts at: 2187 2734 2187 Size: 547 4902 EcoRI G'AATT_C Cuts at: 2758 3136 2758 Size: 378 5071 FspI TGC'GCA Cuts at: 3955 3955 Size: 5449 HaeII r_GCGC'y Cuts at: 1678 2031 2043 2082 2181 4596 1678 Size: 353 12 39 99 2415 2531 Fragments arranged by size: 2531 2415 353 99 39 12 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2603 2635 4090 4122 4164 Size: 32 135 32 1031 32 1455 32 42 Cuts at: 4164 4196 1373 Size: 32 2626 Fragments arranged by size: 2626 1455 1031 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2793 3039 669 Size: 988 281 855 246 3079 Fragments arranged by size: 3079 988 855 281 246 HindIII A'AGCT_T Cuts at: 748 2502 748 Size: 1754 3695 HpaI GTT'AAC Cuts at: 3039 3039 Size: 5449 KasI G'GCGC_C Cuts at: 2027 2039 2078 2177 2027 Size: 12 39 99 5299 Fragments arranged by size: 5299 99 39 12 KpnI G_GTAC'C Cuts at: 2774 2774 Size: 5449 MfeI C'AATT_G Cuts at: 3048 3048 Size: 5449 MluI A'CGCG_T Cuts at: 5325 5325 Size: 5449 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2297 4563 4747 1305 Size: 992 2266 184 2007 Fragments arranged by size: 2266 2007 992 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3627 Fragments arranged by size: 3627 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3407 3766 3925 518 Size: 648 2241 359 159 2042 Fragments arranged by size: 2241 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2740 2865 3542 4690 4935 1606 Size: 1134 125 677 1148 245 2120 Fragments arranged by size: 2120 1148 1134 677 245 125 NaeI GCC'GGC Cuts at: 2038 2077 2083 2038 Size: 39 6 5404 Fragments arranged by size: 5404 39 6 NarI GG'CG_CC Cuts at: 2028 2040 2079 2178 2028 Size: 12 39 99 5299 Fragments arranged by size: 5299 99 39 12 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3958 Fragments arranged by size: 3958 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5449 NgoMIV G'CCGG_C Cuts at: 2036 2075 2081 2036 Size: 39 6 5404 Fragments arranged by size: 5404 39 6 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5449 NotI GC'GGCC_GC Cuts at: 2896 2896 Size: 5449 NspI r_CATG'y Cuts at: 1132 1229 2707 2807 4352 1132 Size: 97 1478 100 1545 2229 Fragments arranged by size: 2229 1545 1478 100 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5449 PciI A'CATG_T Cuts at: 4348 4348 Size: 5449 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5449 PfoI T'CCnGG_A Cuts at: 2166 2166 Size: 5449 PmeI GTTT'AAAC Cuts at: 2753 2753 Size: 5449 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3518 3550 5031 5063 3518 Size: 32 1481 32 3904 Fragments arranged by size: 3904 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5449 PsiI TTA'TAA Cuts at: 3019 3019 Size: 5449 PspOMI G'GGCC_C Cuts at: 2186 2186 Size: 5449 PstI C_TGCA'G Cuts at: 830 2801 830 Size: 1971 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5449 SalI G'TCGA_C Cuts at: 1936 2791 1936 Size: 855 4594 SbfI CC_TGCA'GG Cuts at: 2801 2801 Size: 5449 ScaI AGT'ACT Cuts at: 3697 5409 3697 Size: 1712 3737 SfcI C'TryA_G Cuts at: 826 1045 2465 2566 2797 3932 4613 4804 826 Size: 219 1420 101 231 1135 681 191 1471 Fragments arranged by size: 1471 1420 1135 681 231 219 191 101 SfoI GGC'GCC Cuts at: 2029 2041 2080 2179 2029 Size: 12 39 99 5299 Fragments arranged by size: 5299 99 39 12 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5449 SgrAI Cr'CCGG_yG Cuts at: 1700 2081 1700 Size: 381 5068 SmaI CCC'GGG Cuts at: 1872 2185 2776 1872 Size: 313 591 4545 Fragments arranged by size: 4545 591 313 SmlI C'TyrA_G Cuts at: 820 1017 1943 2726 3553 4454 4716 820 Size: 197 926 783 827 901 262 1553 Fragments arranged by size: 1553 926 901 827 783 262 197 SnaBI TAC'GTA Cuts at: 493 3240 493 Size: 2747 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5449 SphI G_CATG'C Cuts at: 1132 2707 2807 1132 Size: 1575 100 3774 Fragments arranged by size: 3774 1575 100 SspI AAT'ATT Cuts at: 5 52 3373 5 Size: 47 3321 2081 Fragments arranged by size: 3321 2081 47 StuI AGG'CCT Cuts at: 1517 2156 1517 Size: 639 4810 StyI C'CwwG_G Cuts at: 513 1065 2004 2888 513 Size: 552 939 884 3074 Fragments arranged by size: 3074 939 884 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3476 3493 3646 3831 4276 325 Size: 1255 1896 17 153 185 445 1498 Fragments arranged by size: 1896 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3695 5407 96 Size: 274 80 33 51 1443 1718 1712 138 Fragments arranged by size: 1718 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5409 5441 5409 Size: 32 5417 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5449 XbaI T'CTAG_A Cuts at: 2785 2785 Size: 5449 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2264 2631 2011 Size: 253 367 4829 Fragments arranged by size: 4829 367 253 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5449 XmaI C'CCGG_G Cuts at: 1870 2183 2774 1870 Size: 313 591 4545 Fragments arranged by size: 4545 591 313 XmnI GAAnn'nnTTC Cuts at: 3578 3578 Size: 5449 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3257 276 Size: 53 83 186 936 1723 2468 Fragments arranged by size: 2468 1723 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI