(Circular) (Six-base) MAPSORT of: pFN21AB8220.seq Check: 6356 from: 1 to: 6001 pFN21AB8220 6001 bp With 182 enzymes: SgfI * February 27, 2009 14:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2349 3811 278 Size: 53 83 186 936 813 1462 2468 Fragments arranged by size: 2468 1462 936 813 186 83 53 AccI GT'mk_AC Cuts at: 1937 2349 3344 1937 Size: 412 995 4594 Fragments arranged by size: 4594 995 412 Acc65I G'GTAC_C Cuts at: 2772 3322 2772 Size: 550 5451 AclI AA'CG_TT Cuts at: 1563 4128 4501 1563 Size: 2565 373 3063 Fragments arranged by size: 3063 2565 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4064 5448 5716 1632 Size: 2432 1384 268 1917 Fragments arranged by size: 2432 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5804 AflIII A'CryG_T Cuts at: 1203 4900 5877 1203 Size: 3697 977 1327 Fragments arranged by size: 3697 1327 977 AgeI A'CCGG_T Cuts at: 4832 4832 Size: 6001 AhdI GACnn_n'nnGTC Cuts at: 2660 2893 3193 4730 2660 Size: 233 300 1537 3931 Fragments arranged by size: 3931 1537 300 233 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6001 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6001 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2582 2614 2582 Size: 32 5969 AlwNI CAG_nnn'CTG Cuts at: 2446 2804 3058 3744 5316 2446 Size: 358 254 686 1572 3131 Fragments arranged by size: 3131 1572 686 358 254 ApaLI G'TGCA_C Cuts at: 4058 5214 4058 Size: 1156 4845 ApoI r'AATT_y Cuts at: 1493 3310 3536 3688 5797 1493 Size: 1817 226 152 2109 1697 Fragments arranged by size: 2109 1817 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4555 160 Size: 872 3523 1606 Fragments arranged by size: 3523 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6001 AvaI C'yCGr_G Cuts at: 1870 1943 2148 2199 3326 1870 Size: 73 205 51 1127 4545 Fragments arranged by size: 4545 1127 205 73 51 BamHI G'GATC_C Cuts at: 3331 3698 3331 Size: 367 5634 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2105 2772 2862 3322 4777 Size: 325 217 627 318 667 90 460 1455 Cuts at: 4777 618 Size: 1842 Fragments arranged by size: 1842 1455 667 627 460 325 318 217 90 BanII G_rGCy'C Cuts at: 1391 2232 2331 3320 1391 Size: 841 99 989 4072 Fragments arranged by size: 4072 989 841 99 BbeI G_GCGC'C Cuts at: 2866 2866 Size: 6001 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3242 928 Size: 971 1343 3687 Fragments arranged by size: 3687 1343 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4192 4226 1903 Size: 34 2255 34 3678 Fragments arranged by size: 3678 2255 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3893 5103 3893 Size: 1210 4791 BclI T'GATC_A Cuts at: 1548 1548 Size: 6001 BfrBI ATG'CAT Cuts at: 2755 2755 Size: 6001 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2546 4612 136 Size: 107 122 71 631 605 874 2066 1525 Fragments arranged by size: 2066 1525 874 631 605 122 107 71 BglII A'GATC_T Cuts at: 5996 5996 Size: 6001 BlpI GC'TnA_GC Cuts at: 2301 2358 3418 2301 Size: 57 1060 4884 Fragments arranged by size: 4884 1060 57 Bme1580I G_kGCm'C Cuts at: 1790 2176 4062 5218 1790 Size: 386 1886 1156 2573 Fragments arranged by size: 2573 1886 1156 386 BmgBI CAC'GTC Cuts at: 1318 2680 1318 Size: 1362 4639 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3732 4690 442 Size: 416 53 83 399 24 2315 958 1753 Fragments arranged by size: 2315 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6001 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2435 2453 2582 2603 2621 2912 2942 3017 Size: 1196 18 129 21 18 291 30 75 Cuts at: 3017 4661 1239 Size: 1644 2579 Fragments arranged by size: 2579 1644 1196 291 129 75 30 21 18 18 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2623 3002 3200 4126 4991 5289 2623 Size: 379 198 926 865 298 3335 Fragments arranged by size: 3335 926 865 379 298 198 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2189 4664 882 Size: 617 690 2475 2219 Fragments arranged by size: 2475 2219 690 617 BsaAI yAC'GTr Cuts at: 493 1204 2956 3792 493 Size: 711 1752 836 2702 Fragments arranged by size: 2702 1752 836 711 BsaBI GATnn'nnATC Cuts at: 5995 5995 Size: 6001 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2346 2741 2863 3808 Size: 53 83 186 936 813 395 122 945 Cuts at: 3808 4190 4825 275 Size: 382 635 1451 Fragments arranged by size: 1451 945 936 813 635 395 382 186 122 83 53 BsaWI w'CCGG_w Cuts at: 2595 3129 4434 4832 5106 5253 5664 2595 Size: 534 1305 398 274 147 411 2932 Fragments arranged by size: 2932 1305 534 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2582 2612 1178 Size: 30 165 30 1179 30 4567 Fragments arranged by size: 4567 1179 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2026 2041 2600 2603 2633 2636 2960 3137 Size: 840 15 559 3 30 3 324 177 Cuts at: 3137 1186 Size: 4050 Fragments arranged by size: 4050 840 559 324 177 30 15 3 3 BseYI C'CCAG_C Cuts at: 2168 5204 5700 2168 Size: 3036 496 2469 Fragments arranged by size: 3036 2469 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3167 1163 Size: 573 1431 3997 Fragments arranged by size: 3997 1431 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3451 4212 5240 1540 Size: 275 186 1450 761 1028 2301 Fragments arranged by size: 2301 1450 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2232 3288 4062 4147 5218 2232 Size: 1056 774 85 1071 3015 Fragments arranged by size: 3015 1071 1056 774 85 BsmI GAATG_Cn' Cuts at: 2133 3512 3605 2133 Size: 1379 93 4529 Fragments arranged by size: 4529 1379 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2008 2053 2008 Size: 45 5956 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2176 2232 2331 3288 3320 4062 4147 Size: 399 386 56 99 957 32 742 85 Cuts at: 4147 5218 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 957 742 399 386 99 85 56 32 BspEI T'CCGG_A Cuts at: 5664 5664 Size: 6001 BspHI T'CATG_A Cuts at: 3890 5620 3890 Size: 1730 4271 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2650 2891 3066 3171 3356 844 Size: 1806 241 175 105 185 3489 Fragments arranged by size: 3489 1806 241 185 175 105 BsrBI CCG'CTC Cuts at: 1394 1823 2040 2212 2778 2950 3888 1394 Size: 429 217 172 566 172 938 3507 Fragments arranged by size: 3507 938 566 429 217 172 172 BsrDI GCAATG_nn' Cuts at: 65 1248 2742 4496 4670 65 Size: 1183 1494 1754 174 1396 Fragments arranged by size: 1754 1494 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2859 4645 4832 1700 Size: 110 1049 1786 187 2869 Fragments arranged by size: 2869 1786 1049 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6001 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6001 BssSI C'ACGA_G Cuts at: 2615 4061 5073 2615 Size: 1446 1012 3543 Fragments arranged by size: 3543 1446 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2180 794 Size: 1386 4615 BstBI TT'CG_AA Cuts at: 4805 4805 Size: 6001 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6001 BstXI CCAn_nnnn'nTGG Cuts at: 2338 2338 Size: 6001 BstYI r'GATC_y Cuts at: 1972 2557 2592 2610 2715 3331 3698 4083 4100 Size: 585 35 18 105 616 367 385 17 Cuts at: 4100 5552 5638 5650 5996 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 616 585 385 367 346 105 86 35 18 17 12 Bsu36I CC'TnA_GG Cuts at: 2053 2486 2053 Size: 433 5568 BtgI C'CryG_G Cuts at: 513 1065 2004 3240 513 Size: 552 939 1236 3274 Fragments arranged by size: 3274 1236 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5262 Fragments arranged by size: 5262 683 56 BtsI GCAGTG_nn' Cuts at: 3517 4311 4331 3517 Size: 794 20 5187 Fragments arranged by size: 5187 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6001 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5966 DraI TTT'AAA Cuts at: 3305 3305 Size: 6001 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5008 809 Size: 507 3692 1802 Fragments arranged by size: 3692 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2086 2448 2682 Size: 54 1425 50 275 18 256 362 234 Cuts at: 2682 2967 3448 4337 5966 8 Size: 285 481 889 1629 43 Fragments arranged by size: 1629 1425 889 481 362 285 275 256 234 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3448 1537 Size: 275 1636 4090 Fragments arranged by size: 4090 1636 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2760 2997 3931 1353 Size: 1407 237 934 3423 Fragments arranged by size: 3423 1407 934 237 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2819 4588 4962 5108 387 Size: 1064 138 1230 1769 374 146 1280 Fragments arranged by size: 1769 1280 1230 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2230 2230 Size: 6001 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2435 2453 2582 2603 2621 2912 2942 Size: 393 803 18 129 21 18 291 30 Cuts at: 2942 3017 4064 4661 5448 5716 1239 Size: 75 1047 597 787 268 1524 Fragments arranged by size: 1524 1047 803 787 597 393 291 268 129 75 30 21 18 18 EcoO109I rG'GnC_Cy Cuts at: 2074 2099 2443 3055 2074 Size: 25 344 612 5020 Fragments arranged by size: 5020 612 344 25 EcoRI G'AATT_C Cuts at: 3310 3688 3310 Size: 378 5623 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2284 2284 Size: 6001 FspI TGC'GCA Cuts at: 4507 4507 Size: 6001 HaeII r_GCGC'y Cuts at: 1678 2294 2409 2866 2987 5148 1678 Size: 616 115 457 121 2161 2531 Fragments arranged by size: 2531 2161 616 457 121 115 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2416 2448 2646 2678 2879 Size: 32 135 32 844 32 198 32 201 Cuts at: 2879 2911 3179 3211 4642 4674 4716 4748 1373 Size: 32 268 32 1431 32 42 32 2626 Fragments arranged by size: 2626 1431 844 268 201 198 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3345 3591 669 Size: 988 281 1407 246 3079 Fragments arranged by size: 3079 1407 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6001 HpaI GTT'AAC Cuts at: 3591 3591 Size: 6001 KasI G'GCGC_C Cuts at: 2862 2862 Size: 6001 KpnI G_GTAC'C Cuts at: 2776 3326 2776 Size: 550 5451 MfeI C'AATT_G Cuts at: 3600 3600 Size: 6001 MluI A'CGCG_T Cuts at: 5877 5877 Size: 6001 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3183 5115 5299 1305 Size: 1878 1932 184 2007 Fragments arranged by size: 2007 1932 1878 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2450 2684 10 Size: 54 1425 343 618 234 3327 Fragments arranged by size: 3327 1425 618 343 234 54 MslI CAynn'nnrTG Cuts at: 518 1166 3143 3230 3291 3959 4318 4477 518 Size: 648 1977 87 61 668 359 159 2042 Fragments arranged by size: 2042 1977 668 648 359 159 87 61 MspA1I CmG'CkG Cuts at: 1606 2030 2314 2647 3417 4094 5242 5487 1606 Size: 424 284 333 770 677 1148 245 2120 Fragments arranged by size: 2120 1148 770 677 424 333 284 245 NarI GG'CG_CC Cuts at: 2863 2863 Size: 6001 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4510 Fragments arranged by size: 4510 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6001 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6001 NotI GC'GGCC_GC Cuts at: 3448 3448 Size: 6001 NsiI A_TGCA'T Cuts at: 2757 2757 Size: 6001 NspI r_CATG'y Cuts at: 1132 1229 2755 3359 4904 1132 Size: 97 1526 604 1545 2229 Fragments arranged by size: 2229 1545 1526 604 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6001 PciI A'CATG_T Cuts at: 4900 4900 Size: 6001 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6001 PfoI T'CCnGG_A Cuts at: 2271 2469 2637 2271 Size: 198 168 5635 Fragments arranged by size: 5635 198 168 PmeI GTTT'AAAC Cuts at: 3305 3305 Size: 6001 PmlI CAC'GTG Cuts at: 2956 2956 Size: 6001 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2582 2614 4070 4102 5583 5615 2582 Size: 32 1456 32 1481 32 2968 Fragments arranged by size: 2968 1481 1456 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2099 2443 3055 2099 Size: 344 612 5045 Fragments arranged by size: 5045 612 344 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6001 PsiI TTA'TAA Cuts at: 3571 3571 Size: 6001 PstI C_TGCA'G Cuts at: 830 3168 3353 830 Size: 2338 185 3478 Fragments arranged by size: 3478 2338 185 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6001 PvuII CAG'CTG Cuts at: 2314 2647 2314 Size: 333 5668 SacI G_AGCT'C Cuts at: 2232 2232 Size: 6001 SalI G'TCGA_C Cuts at: 1936 3343 1936 Size: 1407 4594 SbfI CC_TGCA'GG Cuts at: 3353 3353 Size: 6001 ScaI AGT'ACT Cuts at: 4249 5961 4249 Size: 1712 4289 SfcI C'TryA_G Cuts at: 826 1045 2518 3100 3164 3349 4484 5165 5356 Size: 219 1473 582 64 185 1135 681 191 Cuts at: 5356 826 Size: 1471 Fragments arranged by size: 1473 1471 1135 681 582 219 191 185 64 SfoI GGC'GCC Cuts at: 2864 2864 Size: 6001 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6001 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6001 SmaI CCC'GGG Cuts at: 1872 2150 2201 3328 1872 Size: 278 51 1127 4545 Fragments arranged by size: 4545 1127 278 51 SmlI C'TyrA_G Cuts at: 820 1017 1943 2602 3017 3215 4105 5006 5268 Size: 197 926 659 415 198 890 901 262 Cuts at: 5268 820 Size: 1553 Fragments arranged by size: 1553 926 901 890 659 415 262 198 197 SnaBI TAC'GTA Cuts at: 493 3792 493 Size: 3299 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6001 SphI G_CATG'C Cuts at: 1132 3359 1132 Size: 2227 3774 SspI AAT'ATT Cuts at: 5 52 3925 5 Size: 47 3873 2081 Fragments arranged by size: 3873 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6001 StyI C'CwwG_G Cuts at: 513 1065 2004 2322 3440 513 Size: 552 939 318 1118 3074 Fragments arranged by size: 3074 1118 939 552 318 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4028 4045 4198 4383 4828 325 Size: 1255 2448 17 153 185 445 1498 Fragments arranged by size: 2448 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4247 5959 96 Size: 274 80 33 51 1443 2270 1712 138 Fragments arranged by size: 2270 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5961 5993 5961 Size: 32 5969 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6001 XbaI T'CTAG_A Cuts at: 3337 3337 Size: 6001 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2488 2488 Size: 6001 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6001 XmaI C'CCGG_G Cuts at: 1870 2148 2199 3326 1870 Size: 278 51 1127 4545 Fragments arranged by size: 4545 1127 278 51 XmnI GAAnn'nnTTC Cuts at: 4130 4130 Size: 6001 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2347 3809 276 Size: 53 83 186 936 813 1462 2468 Fragments arranged by size: 2468 1462 936 813 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbvCI BplI Bpu10I BsiWI BstZ17I DraIII EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI PacI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI