(Circular) (Six-base) MAPSORT of: pFN21AB8205.seq Check: 7325 from: 1 to: 5758 pFN21AB8205 5758 bp With 182 enzymes: SgfI * February 27, 2009 14:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3568 278 Size: 53 83 186 936 2032 2468 Fragments arranged by size: 2468 2032 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3101 1937 Size: 1164 4594 Acc65I G'GTAC_C Cuts at: 2499 2865 3079 2499 Size: 366 214 5178 Fragments arranged by size: 5178 366 214 AclI AA'CG_TT Cuts at: 1563 3885 4258 1563 Size: 2322 373 3063 Fragments arranged by size: 3063 2322 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3821 5205 5473 1632 Size: 2189 1384 268 1917 Fragments arranged by size: 2189 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5561 AflIII A'CryG_T Cuts at: 1203 4657 5634 1203 Size: 3454 977 1327 Fragments arranged by size: 3454 1327 977 AgeI A'CCGG_T Cuts at: 4589 4589 Size: 5758 AhdI GACnn_n'nnGTC Cuts at: 4487 4487 Size: 5758 AleI CACnn'nnGTG Cuts at: 1166 2447 1166 Size: 1281 4477 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5758 AlwNI CAG_nnn'CTG Cuts at: 2845 3005 3501 5073 2845 Size: 160 496 1572 3530 Fragments arranged by size: 3530 1572 496 160 ApaI G_GGCC'C Cuts at: 2309 2755 2309 Size: 446 5312 ApaLI G'TGCA_C Cuts at: 3815 4971 3815 Size: 1156 4602 ApoI r'AATT_y Cuts at: 1493 3067 3293 3445 5554 1493 Size: 1574 226 152 2109 1697 Fragments arranged by size: 2109 1697 1574 226 152 AseI AT'TA_AT Cuts at: 160 1032 4312 160 Size: 872 3280 1606 Fragments arranged by size: 3280 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5758 AvaI C'yCGr_G Cuts at: 1870 1943 2360 3083 1870 Size: 73 417 723 4545 Fragments arranged by size: 4545 723 417 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2483 2516 2483 Size: 33 5725 BamHI G'GATC_C Cuts at: 2569 2598 3088 3455 2569 Size: 29 490 367 4872 Fragments arranged by size: 4872 490 367 29 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2008 2124 2145 2153 2449 Size: 325 217 627 221 116 21 8 296 Cuts at: 2449 2499 2514 2760 2865 3079 4534 618 Size: 50 15 246 105 214 1455 1842 Fragments arranged by size: 1842 1455 627 325 296 246 221 217 214 116 105 50 21 15 8 BanII G_rGCy'C Cuts at: 1391 2067 2309 2346 2755 3077 1391 Size: 676 242 37 409 322 4072 Fragments arranged by size: 4072 676 409 322 242 37 BbeI G_GCGC'C Cuts at: 2012 2518 2012 Size: 506 5252 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4787 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3949 3983 1903 Size: 34 2012 34 3678 Fragments arranged by size: 3678 2012 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3650 4860 3650 Size: 1210 4548 BclI T'GATC_A Cuts at: 1548 1548 Size: 5758 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2258 4369 136 Size: 107 122 71 631 605 586 2111 1525 Fragments arranged by size: 2111 1525 631 605 586 122 107 71 BglII A'GATC_T Cuts at: 5753 5753 Size: 5758 BlpI GC'TnA_GC Cuts at: 3175 3175 Size: 5758 Bme1580I G_kGCm'C Cuts at: 1790 2127 2231 2309 2460 2755 3819 4975 1790 Size: 337 104 78 151 295 1064 1156 2573 Fragments arranged by size: 2573 1156 1064 337 295 151 104 78 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5758 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2816 2934 3489 Size: 416 53 83 399 24 1399 118 555 Cuts at: 3489 4447 442 Size: 958 1753 Fragments arranged by size: 1753 1399 958 555 416 399 118 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2369 1056 Size: 1313 4445 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2423 2798 2903 4418 1239 Size: 1184 375 105 1515 2579 Fragments arranged by size: 2579 1515 1184 375 105 Bpu10I CC'TnA_GC Cuts at: 2141 2141 Size: 5758 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3883 4748 5046 3883 Size: 865 298 4595 Fragments arranged by size: 4595 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2912 4421 882 Size: 617 1413 1509 2219 Fragments arranged by size: 2219 1509 1413 617 BsaAI yAC'GTr Cuts at: 493 1204 3549 493 Size: 711 2345 2702 Fragments arranged by size: 2702 2345 711 BsaBI GATnn'nnATC Cuts at: 5752 5752 Size: 5758 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2009 2103 2515 3565 Size: 53 83 186 936 476 94 412 1050 Cuts at: 3565 3947 4582 275 Size: 382 635 1451 Fragments arranged by size: 1451 1050 936 635 476 412 382 186 94 83 53 BsaWI w'CCGG_w Cuts at: 2595 4191 4589 4863 5010 5421 2595 Size: 1596 398 274 147 411 2932 Fragments arranged by size: 2932 1596 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5533 Fragments arranged by size: 5533 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2063 2102 2420 2928 3023 1186 Size: 877 39 318 508 95 3921 Fragments arranged by size: 3921 877 508 318 95 39 BseYI C'CCAG_C Cuts at: 4961 5457 4961 Size: 496 5262 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5185 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3208 3969 4997 1540 Size: 275 186 1207 761 1028 2301 Fragments arranged by size: 2301 1207 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2784 2853 3819 3904 4975 2784 Size: 69 966 85 1071 3567 Fragments arranged by size: 3567 1071 966 85 69 BsmI GAATG_Cn' Cuts at: 2275 3269 3362 2275 Size: 994 93 4671 Fragments arranged by size: 4671 994 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2067 2127 2231 2309 2346 2460 2755 Size: 399 277 60 104 78 37 114 295 Cuts at: 2755 2784 2853 3077 3819 3904 4975 1391 Size: 29 69 224 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 295 277 224 114 104 85 78 69 60 37 29 BspEI T'CCGG_A Cuts at: 5421 5421 Size: 5758 BspHI T'CATG_A Cuts at: 3647 5377 3647 Size: 1730 4028 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2877 3113 844 Size: 2033 236 3489 Fragments arranged by size: 3489 2033 236 BsrBI CCG'CTC Cuts at: 1394 1823 3645 1394 Size: 429 1822 3507 Fragments arranged by size: 3507 1822 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2614 4253 4427 65 Size: 1183 1366 1639 174 1396 Fragments arranged by size: 1639 1396 1366 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2011 4402 4589 1700 Size: 110 201 2391 187 2869 Fragments arranged by size: 2869 2391 201 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5758 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5758 BssSI C'ACGA_G Cuts at: 3818 4830 3818 Size: 1012 4746 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5758 BstBI TT'CG_AA Cuts at: 4562 4562 Size: 5758 BstEII G'GTnAC_C Cuts at: 1184 2576 1184 Size: 1392 4366 BstXI CCAn_nnnn'nTGG Cuts at: 2476 2476 Size: 5758 BstYI r'GATC_y Cuts at: 1972 2569 2598 3043 3088 3455 3840 3857 5309 Size: 597 29 445 45 367 385 17 1452 Cuts at: 5309 5395 5407 5753 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 597 445 385 367 346 86 45 29 17 12 Bsu36I CC'TnA_GG Cuts at: 3006 3006 Size: 5758 BtgI C'CryG_G Cuts at: 513 1065 2004 2518 513 Size: 552 939 514 3753 Fragments arranged by size: 3753 939 552 514 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5019 Fragments arranged by size: 5019 683 56 BtsI GCAGTG_nn' Cuts at: 2503 3274 4068 4088 2503 Size: 771 794 20 4173 Fragments arranged by size: 4173 794 771 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5758 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5723 DraI TTT'AAA Cuts at: 3062 3062 Size: 5758 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4765 809 Size: 507 3449 1802 Fragments arranged by size: 3449 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3205 4094 5723 Size: 54 1425 50 275 18 1375 889 1629 Cuts at: 5723 8 Size: 43 Fragments arranged by size: 1629 1425 1375 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3205 1537 Size: 275 1393 4090 Fragments arranged by size: 4090 1393 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3688 1353 Size: 2335 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2039 4345 4719 4865 387 Size: 1064 138 450 2306 374 146 1280 Fragments arranged by size: 2306 1280 1064 450 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2423 2798 2903 3821 4418 5205 5473 Size: 393 791 375 105 918 597 787 268 Cuts at: 5473 1239 Size: 1524 Fragments arranged by size: 1524 918 791 787 597 393 375 268 105 EcoNI CCTnn'n_nnAGG Cuts at: 2192 2837 2192 Size: 645 5113 EcoO109I rG'GnC_Cy Cuts at: 2299 2410 2638 3013 2299 Size: 111 228 375 5044 Fragments arranged by size: 5044 375 228 111 EcoRI G'AATT_C Cuts at: 3067 3445 3067 Size: 378 5380 FspI TGC'GCA Cuts at: 4264 4264 Size: 5758 HaeII r_GCGC'y Cuts at: 1678 2012 2093 2402 2518 4905 1678 Size: 334 81 309 116 2387 2531 Fragments arranged by size: 2531 2387 334 309 116 81 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3032 3064 4399 4431 4473 Size: 32 135 32 1460 32 1335 32 42 Cuts at: 4473 4505 1373 Size: 32 2626 Fragments arranged by size: 2626 1460 1335 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3102 3348 669 Size: 988 281 1164 246 3079 Fragments arranged by size: 3079 1164 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5758 HpaI GTT'AAC Cuts at: 3348 3348 Size: 5758 KasI G'GCGC_C Cuts at: 2008 2514 2008 Size: 506 5252 KpnI G_GTAC'C Cuts at: 2503 2869 3083 2503 Size: 366 214 5178 Fragments arranged by size: 5178 366 214 MfeI C'AATT_G Cuts at: 3357 3357 Size: 5758 MluI A'CGCG_T Cuts at: 5634 5634 Size: 5758 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4872 5056 1305 Size: 3567 184 2007 Fragments arranged by size: 3567 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3936 Fragments arranged by size: 3936 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2447 3716 4075 4234 518 Size: 648 1281 1269 359 159 2042 Fragments arranged by size: 2042 1281 1269 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2210 2311 3174 3851 4999 5244 1606 Size: 604 101 863 677 1148 245 2120 Fragments arranged by size: 2120 1148 863 677 604 245 101 NarI GG'CG_CC Cuts at: 2009 2515 2009 Size: 506 5252 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4267 Fragments arranged by size: 4267 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5758 NheI G'CTAG_C Cuts at: 1052 2365 1052 Size: 1313 4445 NotI GC'GGCC_GC Cuts at: 3205 3205 Size: 5758 NspI r_CATG'y Cuts at: 1132 1229 2818 3116 4661 1132 Size: 97 1589 298 1545 2229 Fragments arranged by size: 2229 1589 1545 298 97 PasI CC'CwG_GG Cuts at: 1348 2923 1348 Size: 1575 4183 PciI A'CATG_T Cuts at: 4657 4657 Size: 5758 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5758 PmeI GTTT'AAAC Cuts at: 3062 3062 Size: 5758 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3827 3859 5340 5372 3827 Size: 32 1481 32 4213 Fragments arranged by size: 4213 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2299 2638 2299 Size: 339 5419 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5758 PsiI TTA'TAA Cuts at: 3328 3328 Size: 5758 PspOMI G'GGCC_C Cuts at: 2305 2751 2305 Size: 446 5312 PstI C_TGCA'G Cuts at: 830 2040 2841 2996 3110 830 Size: 1210 801 155 114 3478 Fragments arranged by size: 3478 1210 801 155 114 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5758 SalI G'TCGA_C Cuts at: 1936 3100 1936 Size: 1164 4594 SanDI GG'GwC_CC Cuts at: 2638 2638 Size: 5758 SbfI CC_TGCA'GG Cuts at: 2996 3110 2996 Size: 114 5644 ScaI AGT'ACT Cuts at: 4006 5718 4006 Size: 1712 4046 SfcI C'TryA_G Cuts at: 826 1045 2036 2116 2837 2975 2992 3106 4241 Size: 219 991 80 721 138 17 114 1135 Cuts at: 4241 4922 5113 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 991 721 681 219 191 138 114 80 17 SfoI GGC'GCC Cuts at: 2010 2516 2010 Size: 506 5252 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5758 SgrAI Cr'CCGG_yG Cuts at: 1700 2011 1700 Size: 311 5447 SmaI CCC'GGG Cuts at: 1872 3085 1872 Size: 1213 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3862 4763 5025 820 Size: 197 926 1919 901 262 1553 Fragments arranged by size: 1919 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3549 493 Size: 3056 2702 SpeI A'CTAG_T Cuts at: 152 2236 152 Size: 2084 3674 SphI G_CATG'C Cuts at: 1132 2818 3116 1132 Size: 1686 298 3774 Fragments arranged by size: 3774 1686 298 SspI AAT'ATT Cuts at: 5 52 3682 5 Size: 47 3630 2081 Fragments arranged by size: 3630 2081 47 StuI AGG'CCT Cuts at: 1517 2619 1517 Size: 1102 4656 StyI C'CwwG_G Cuts at: 513 1065 2004 2149 2424 2755 2854 3036 3197 Size: 552 939 145 275 331 99 182 161 Cuts at: 3197 513 Size: 3074 Fragments arranged by size: 3074 939 552 331 275 182 161 145 99 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2231 3785 3802 3955 4140 4585 325 Size: 1255 651 1554 17 153 185 445 1498 Fragments arranged by size: 1554 1498 1255 651 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4004 5716 96 Size: 274 80 33 51 1443 2027 1712 138 Fragments arranged by size: 2027 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5718 5750 5718 Size: 32 5726 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5758 XbaI T'CTAG_A Cuts at: 3094 3094 Size: 5758 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2476 2476 Size: 5758 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5758 XmaI C'CCGG_G Cuts at: 1870 3083 1870 Size: 1213 4545 XmnI GAAnn'nnTTC Cuts at: 3887 3887 Size: 5758 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3566 276 Size: 53 83 186 936 2032 2468 Fragments arranged by size: 2468 2032 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI SacII SapI SexAI SfiI SrfI SwaI