Restriction map for
pFN21AB8202
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# NO CUT ENZYMES ( 171 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BalI BanI BbeI BbrPI
BbsI BbvCI BcgI BciVI BfaI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspLU11I BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT14I
FseI FspI HaeII HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI Ksp632I MaeI MaeII MamI
MfeI MluI MluNI MroI MscI
MseI MslI MunI NarI NcoI
NdeI NheI NotI NruI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TaqI
Tru9I Tsp45I Tth111I TthHB8I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AfaI 548 GTAC GT^AC
AlwNI 173 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
BamHI 555 GGATCC G^GATCC
BanII 26 GGGCTC G[GA]GC[CT]^C
BclI 248 TGATCA T^GATCA
BcnI 118 CCCGG CC^[GC]GG
BmyI 26 GGGCTC G[AGT]GC[ACT]^C
BseRI 152 CTCCTC GAGGAG(10/8)
Bsp1286I 26 GGGCTC G[AGT]GC[ACT]^C
BspHI 200 TCATGA T^CATGA
BspMI 332 ACCTGC ACCTGC(4/8)
BsrBI 381 CCGCTC CCGCTC(-3/-3)
BsrFI 448 GCCGGC [GA]^CCGG[CT]
BstF5I 341 GGATG GGATG(2/0)
BstYI 555 GGATCC [GA]^GATC[CT]
CfoI 161 GCGC GCG^C
Cfr10I 448 GCCGGC [GA]^CCGG[CT]
DraII 114 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoNI 427 CCTATCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 114 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoT22I 496 ATGCAT ATGCA^T
FbaI 248 TGATCA T^GATCA
FokI 341 GGATG GGATG(9/13)
HhaI 161 GCGC GCG^C
HinP1I 161 GCGC G^CGC
HinfI 243 GAATC G^A[ACGT]TC
HphI 300 GGTGA GGTGA(8/7)
KspI 140 CCGCGG CCGC^GG
MaeIII 317 GTAAC ^GT[ACGT]AC
MflI 555 GGATCC [GA]^GATC[CT]
MspA1I 140 CCGCGG C[AC]G^C[GT]G
NaeI 448 GCCGGC GCC^GGC
NciI 118 CCCGG CC^[GC]GG
NgoMIV 448 GCCGGC G^CCGGC
NsiI 496 ATGCAT ATGCA^T
PpuMI 114 GGGACCC [GA]G^G[AT]CC[CT]
RcaI 200 TCATGA T^CATGA
RsaI 548 GTAC GT^AC
SacII 140 CCGCGG CCGC^GG
SfaNI 495 GATGC GCATC(5/9)
TfiI 243 GAATC G^A[AT]TC
XhoII 555 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AccII 141 CGCG CG^CG
162 CGCG
AcsI 6 GAATTT [GA]^AATT[CT]
283 AAATTC
AlwI 556 GATCC GGATC(4/5)
555 GGATC
ApoI 6 GAATTT [GA]^AATT[CT]
283 AAATTC
BbvI 236 GCAGC GCAGC(8/12)
374 GCAGC
BsrI 466 ACTGG ACTGG(1/-1)
578 CCAGT
BstUI 141 CGCG CG^CG
162 CGCG
DsaI 140 CCGCGG C^C[GA][CT]GG
146 CCACGG
EaeI 143 CGGCCA [CT]^GGCC[GA]
446 TGGCCG
MvnI 141 CGCG CG^CG
162 CGCG
TseI 236 GCAGC G^C[AT]GC
374 GCAGC
Tsp509I 7 AATT ^AATT
284 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AciI AluI AvaII BsaJI BsiYI
BslI BsmFI BssKI BstNI Cac8I
Cfr13I DdeI DpnII EcoRII FauI
Fnu4HI HaeIII HapII HpaII ItaI
MboI MboII MnlI MspI MvaI
MwoI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI TspRI