(Circular) (Six-base) MAPSORT of: pFN21AB8200.seq Check: 8876 from: 1 to: 5821 pFN21AB8200 5821 bp With 182 enzymes: SgfI * February 27, 2009 14:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2155 2155 Size: 5821 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3631 278 Size: 53 83 186 936 2095 2468 Fragments arranged by size: 2468 2095 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3164 1937 Size: 1227 4594 Acc65I G'GTAC_C Cuts at: 3142 3142 Size: 5821 AclI AA'CG_TT Cuts at: 1563 3948 4321 1563 Size: 2385 373 3063 Fragments arranged by size: 3063 2385 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2267 3884 5268 5536 1632 Size: 635 1617 1384 268 1917 Fragments arranged by size: 1917 1617 1384 635 268 AfeI AGC'GCT Cuts at: 2906 2906 Size: 5821 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5624 AflIII A'CryG_T Cuts at: 1203 2235 4720 5697 1203 Size: 1032 2485 977 1327 Fragments arranged by size: 2485 1327 1032 977 AgeI A'CCGG_T Cuts at: 4652 4652 Size: 5821 AhdI GACnn_n'nnGTC Cuts at: 4550 4550 Size: 5821 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5821 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5821 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2573 2605 2573 Size: 32 5789 AlwNI CAG_nnn'CTG Cuts at: 2607 2917 3564 5136 2607 Size: 310 647 1572 3292 Fragments arranged by size: 3292 1572 647 310 ApaI G_GGCC'C Cuts at: 2188 2320 2188 Size: 132 5689 ApaLI G'TGCA_C Cuts at: 2138 3878 5034 2138 Size: 1740 1156 2925 Fragments arranged by size: 2925 1740 1156 ApoI r'AATT_y Cuts at: 1493 3130 3356 3508 5617 1493 Size: 1637 226 152 2109 1697 Fragments arranged by size: 2109 1697 1637 226 152 AseI AT'TA_AT Cuts at: 160 1032 4375 160 Size: 872 3343 1606 Fragments arranged by size: 3343 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5821 AvaI C'yCGr_G Cuts at: 1870 1943 2189 3146 1870 Size: 73 246 957 4545 Fragments arranged by size: 4545 957 246 73 BamHI G'GATC_C Cuts at: 3151 3518 3151 Size: 367 5454 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2120 2454 2545 3142 4597 Size: 325 217 627 333 334 91 597 1455 Cuts at: 4597 618 Size: 1842 Fragments arranged by size: 1842 1455 627 597 334 333 325 217 91 BanII G_rGCy'C Cuts at: 1391 2034 2148 2188 2320 2564 2879 2936 3140 Size: 643 114 40 132 244 315 57 204 Cuts at: 3140 1391 Size: 4072 Fragments arranged by size: 4072 643 315 244 204 132 114 57 40 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4850 BbvCI CC'TCA_GC Cuts at: 2441 2441 Size: 5821 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4012 4046 1903 Size: 34 2075 34 3678 Fragments arranged by size: 3678 2075 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3713 4923 3713 Size: 1210 4611 BclI T'GATC_A Cuts at: 1548 1548 Size: 5821 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4432 136 Size: 107 122 71 631 605 2760 1525 Fragments arranged by size: 2760 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2997 5816 2997 Size: 2819 3002 BlpI GC'TnA_GC Cuts at: 2840 3008 3238 2840 Size: 168 230 5423 Fragments arranged by size: 5423 230 168 Bme1580I G_kGCm'C Cuts at: 1790 2142 2188 2320 3882 5038 1790 Size: 352 46 132 1562 1156 2573 Fragments arranged by size: 2573 1562 1156 352 132 46 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5821 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2191 3552 4510 Size: 416 53 83 399 24 774 1361 958 Cuts at: 4510 442 Size: 1753 Fragments arranged by size: 1753 1361 958 774 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5821 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4481 1239 Size: 3242 2579 Bpu10I CC'TnA_GC Cuts at: 2441 2868 2441 Size: 427 5394 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2132 3095 3946 4811 5109 2132 Size: 963 851 865 298 2844 Fragments arranged by size: 2844 963 865 851 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4484 882 Size: 617 2985 2219 Fragments arranged by size: 2985 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2236 3612 493 Size: 711 1032 1376 2702 Fragments arranged by size: 2702 1376 1032 711 BsaBI GATnn'nnATC Cuts at: 2075 2366 5815 2075 Size: 291 3449 2081 Fragments arranged by size: 3449 2081 291 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2625 2823 3628 4010 Size: 53 83 186 936 1092 198 805 382 Cuts at: 4010 4645 275 Size: 635 1451 Fragments arranged by size: 1451 1092 936 805 635 382 198 186 83 53 BsaWI w'CCGG_w Cuts at: 4254 4652 4926 5073 5484 4254 Size: 398 274 147 411 4591 Fragments arranged by size: 4591 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5596 Fragments arranged by size: 5596 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5821 BseYI C'CCAG_C Cuts at: 2313 2613 5024 5520 2313 Size: 300 2411 496 2614 Fragments arranged by size: 2614 2411 496 300 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2960 3025 1163 Size: 573 1224 65 3959 Fragments arranged by size: 3959 1224 573 65 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2543 3271 4032 5060 1540 Size: 275 186 542 728 761 1028 2301 Fragments arranged by size: 2301 1028 761 728 542 275 186 BsiHKAI G_wGCw'C Cuts at: 2142 2148 2157 2879 2936 3009 3032 3882 3967 Size: 6 9 722 57 73 23 850 85 Cuts at: 3967 5038 2142 Size: 1071 2925 Fragments arranged by size: 2925 1071 850 722 85 73 57 23 9 6 BsmI GAATG_Cn' Cuts at: 3332 3425 3332 Size: 93 5728 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2034 2142 2148 2157 2188 2320 2564 Size: 399 244 108 6 9 31 132 244 Cuts at: 2564 2879 2936 3009 3032 3140 3882 3967 5038 Size: 315 57 73 23 108 742 85 1071 Cuts at: 5038 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 315 244 244 132 108 108 85 73 57 31 23 9 6 BspEI T'CCGG_A Cuts at: 5484 5484 Size: 5821 BspHI T'CATG_A Cuts at: 3710 5440 3710 Size: 1730 4091 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2155 2219 3176 844 Size: 1311 64 957 3489 Fragments arranged by size: 3489 1311 957 64 BsrBI CCG'CTC Cuts at: 1394 1823 2118 2430 3708 1394 Size: 429 295 312 1278 3507 Fragments arranged by size: 3507 1278 429 312 295 BsrDI GCAATG_nn' Cuts at: 65 1248 4316 4490 65 Size: 1183 3068 174 1396 Fragments arranged by size: 3068 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2480 2542 4465 4652 1700 Size: 110 670 62 1923 187 2869 Fragments arranged by size: 2869 1923 670 187 110 62 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5821 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5821 BssSI C'ACGA_G Cuts at: 2141 2355 3881 4893 2141 Size: 214 1526 1012 3069 Fragments arranged by size: 3069 1526 1012 214 BstAPI GCAn_nnn'nTGC Cuts at: 794 2607 2682 794 Size: 1813 75 3933 Fragments arranged by size: 3933 1813 75 BstBI TT'CG_AA Cuts at: 2592 4625 2592 Size: 2033 3788 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5821 BstXI CCAn_nnnn'nTGG Cuts at: 3052 3052 Size: 5821 BstYI r'GATC_y Cuts at: 1972 2505 2997 3151 3518 3903 3920 5372 5458 Size: 533 492 154 367 385 17 1452 86 Cuts at: 5458 5470 5816 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 533 492 385 367 346 154 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2555 2829 513 Size: 552 939 551 274 3505 Fragments arranged by size: 3505 939 552 551 274 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5082 Fragments arranged by size: 5082 683 56 BtsI GCAGTG_nn' Cuts at: 2741 3337 4131 4151 2741 Size: 596 794 20 4411 Fragments arranged by size: 4411 794 596 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5821 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5786 DraI TTT'AAA Cuts at: 3125 3125 Size: 5821 DraIII CAC_nnn'GTG Cuts at: 2556 2556 Size: 5821 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2678 4828 809 Size: 507 1362 2150 1802 Fragments arranged by size: 2150 1802 1362 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2540 2709 3268 Size: 54 1425 50 275 18 710 169 559 Cuts at: 3268 4157 5786 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 710 559 275 169 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2540 3268 1537 Size: 275 728 728 4090 Fragments arranged by size: 4090 728 728 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3077 3751 1353 Size: 1724 674 3423 Fragments arranged by size: 3423 1724 674 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4408 4782 4928 387 Size: 1064 138 2819 374 146 1280 Fragments arranged by size: 2819 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2146 2877 2934 2146 Size: 731 57 5033 Fragments arranged by size: 5033 731 57 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2267 3884 4481 5268 5536 1239 Size: 393 635 1617 597 787 268 1524 Fragments arranged by size: 1617 1524 787 635 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2185 2317 3106 2185 Size: 132 789 4900 Fragments arranged by size: 4900 789 132 EcoRI G'AATT_C Cuts at: 3130 3508 3130 Size: 378 5443 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2590 2590 Size: 5821 FspI TGC'GCA Cuts at: 4327 4327 Size: 5821 HaeII r_GCGC'y Cuts at: 1678 2753 2908 4968 1678 Size: 1075 155 2060 2531 Fragments arranged by size: 2531 2060 1075 155 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4462 4494 4536 4568 1373 Size: 32 135 32 2890 32 42 32 2626 Fragments arranged by size: 2890 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3165 3411 669 Size: 988 281 1227 246 3079 Fragments arranged by size: 3079 1227 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5821 HpaI GTT'AAC Cuts at: 3411 3411 Size: 5821 KpnI G_GTAC'C Cuts at: 3146 3146 Size: 5821 MfeI C'AATT_G Cuts at: 3420 3420 Size: 5821 MluI A'CGCG_T Cuts at: 5697 5697 Size: 5821 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2483 4935 5119 1305 Size: 1178 2452 184 2007 Fragments arranged by size: 2452 2007 1178 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3999 Fragments arranged by size: 3999 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2106 3050 3779 4138 4297 518 Size: 648 940 944 729 359 159 2042 Fragments arranged by size: 2042 944 940 729 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2040 2304 2349 2643 2786 2831 3012 3237 Size: 434 264 45 294 143 45 181 225 Cuts at: 3237 3914 5062 5307 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 434 294 264 245 225 181 143 45 45 NaeI GCC'GGC Cuts at: 2482 2544 2482 Size: 62 5759 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4330 Fragments arranged by size: 4330 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5821 NgoMIV G'CCGG_C Cuts at: 2480 2542 2480 Size: 62 5759 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5821 NotI GC'GGCC_GC Cuts at: 3268 3268 Size: 5821 NruI TCG'CGA Cuts at: 2717 2717 Size: 5821 NspI r_CATG'y Cuts at: 1132 1229 3179 4724 1132 Size: 97 1950 1545 2229 Fragments arranged by size: 2229 1950 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5821 PciI A'CATG_T Cuts at: 4720 4720 Size: 5821 PflMI CCAn_nnn'nTGG Cuts at: 1108 2556 2917 1108 Size: 1448 361 4012 Fragments arranged by size: 4012 1448 361 PmeI GTTT'AAAC Cuts at: 3125 3125 Size: 5821 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3890 3922 5403 5435 3890 Size: 32 1481 32 4276 Fragments arranged by size: 4276 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3106 3106 Size: 5821 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5821 PsiI TTA'TAA Cuts at: 3391 3391 Size: 5821 PspOMI G'GGCC_C Cuts at: 2184 2316 2184 Size: 132 5689 PstI C_TGCA'G Cuts at: 830 3173 830 Size: 2343 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5821 PvuII CAG'CTG Cuts at: 2040 2040 Size: 5821 SacI G_AGCT'C Cuts at: 2148 2879 2936 2148 Size: 731 57 5033 Fragments arranged by size: 5033 731 57 SacII CC_GC'GG Cuts at: 2832 2832 Size: 5821 SalI G'TCGA_C Cuts at: 1936 3163 1936 Size: 1227 4594 SapI GCTCTTCn'nnn_ Cuts at: 3077 3077 Size: 5821 SbfI CC_TGCA'GG Cuts at: 3173 3173 Size: 5821 ScaI AGT'ACT Cuts at: 4069 5781 4069 Size: 1712 4109 SfcI C'TryA_G Cuts at: 826 1045 3169 4304 4985 5176 826 Size: 219 2124 1135 681 191 1471 Fragments arranged by size: 2124 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5821 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5821 SmaI CCC'GGG Cuts at: 1872 3148 1872 Size: 1276 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2147 3110 3925 4826 5088 820 Size: 197 926 204 963 815 901 262 1553 Fragments arranged by size: 1553 963 926 901 815 262 204 197 SnaBI TAC'GTA Cuts at: 493 3612 493 Size: 3119 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5821 SphI G_CATG'C Cuts at: 1132 3179 1132 Size: 2047 3774 SspI AAT'ATT Cuts at: 5 52 3745 5 Size: 47 3693 2081 Fragments arranged by size: 3693 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5821 StyI C'CwwG_G Cuts at: 513 1065 2004 2133 3260 513 Size: 552 939 129 1127 3074 Fragments arranged by size: 3074 1127 939 552 129 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2081 2563 3848 3865 4018 4203 4648 Size: 1255 501 482 1285 17 153 185 445 Cuts at: 4648 325 Size: 1498 Fragments arranged by size: 1498 1285 1255 501 482 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4067 5779 96 Size: 274 80 33 51 1443 2090 1712 138 Fragments arranged by size: 2090 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5781 5813 5781 Size: 32 5789 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5821 XbaI T'CTAG_A Cuts at: 3157 3157 Size: 5821 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5821 XmaI C'CCGG_G Cuts at: 1870 3146 1870 Size: 1276 4545 XmnI GAAnn'nnTTC Cuts at: 3950 3950 Size: 5821 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3629 276 Size: 53 83 186 936 2095 2468 Fragments arranged by size: 2468 2095 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI KasI NarI NsiI PacI PfoI PmlI PspXI PsrI RsrII SanDI SexAI SfiI SfoI SrfI SwaI XcmI