Restriction map for
pFN21AB8187
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI AspEI AvaI AviII AvrII
BalI BbeI BbrPI BbvCI BciVI
BfrI BlnI BsaAI BsaHI BsaI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI BsoBI BspDI BspEI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
CfoI Cfr10I ClaI CpoI DraI
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HaeII
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI KasI KspI MaeII
MfeI MluI MluNI MroI MscI
MunI MvnI NaeI NarI NcoI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflMI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RsrII
SacI SacII SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
SwaI Van91I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 57 enzymes) #
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Acc65I 1167 GGTACC G^GTACC
AcsI 1312 AAATTT [GA]^AATT[CT]
AflIII 1121 ACATGT A^C[GA][CT]GT
AlwNI 378 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 368 GGGCCC GGGCC^C
ApoI 1312 AAATTT [GA]^AATT[CT]
Asp700I 1219 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1167 GGTACC G^GTACC
AspHI 748 GTGCTC G[AT]GC[AT]^C
AspI 646 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 823 GGATCC G^GATCC
BbvI 220 GCAGC GCAGC(8/12)
BclI 158 TGATCA T^GATCA
BglII 1277 AGATCT A^GATCT
BlpI 1112 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1112 GCTCAGC GC^T[ACGT]AGC
BsaBI 153 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 785 CTCCTC GAGGAG(10/8)
BsgI 310 CTGCAC GTGCAG(16/14)
BsiHKAI 748 GTGCTC G[AT]GC[AT]^C
Bsp1407I 246 TGTACA T^GTACA
BspHI 1271 TCATGA T^CATGA
BspLU11I 1121 ACATGT A^CATGT
BsrDI 641 GCAATG GCAATG(2/0)
BsrGI 246 TGTACA T^GTACA
BstXI 1032 CCAATGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1112 GCTCAGC GC^T[ACGT]AGC
DraIII 15 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 192 CCGTGG C^C[GA][CT]GG
EcoNI 78 CCTCAACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 419 CCAAGG C^C[AT][AT]GG
FbaI 158 TGATCA T^GATCA
HgaI 1109 GACGC GACGC(5/10)
HphI 1155 GGTGA GGTGA(8/7)
KpnI 1167 GGTACC GGTAC^C
MamI 153 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 578 TTAA T^TAA
MspA1I 630 CCGCTG C[AC]G^C[GT]G
NdeI 818 CATATG CA^TATG
PflFI 646 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 350 AGGACCT [GA]G^G[AT]CC[CT]
PstI 1081 CTGCAG CTGCA^G
RcaI 1271 TCATGA T^CATGA
SapI 690 GAAGAGC GCTCTTC(1/4)
SfcI 1081 CTGCAG C^T[GA][CT]AG
SmlI 1336 CTTGAG C^T[CT][GA]AG
SspBI 246 TGTACA T^GTACA
StuI 1221 AGGCCT AGG^CCT
StyI 419 CCAAGG C^C[AT][AT]GG
TaqI 672 TCGA T^CGA
Tru9I 578 TTAA T^TAA
TseI 220 GCAGC G^C[AT]GC
Tsp45I 1156 GTGAC ^GT[GC]AC
Tth111I 646 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 672 TCGA T^CGA
XcmI 854 CCAATTTCGTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1219 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
BanII 368 GGGCCC G[GA]GC[CT]^C
934 GAGCCC
BbsI 1096 GAAGAC GAAGAC(2/6)
1291 GAAGAC
BcgI 32 GCAACGCAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
851 GCACCAATTTCG
BcnI 24 CCGGG CC^[GC]GG
719 CCCGG
BfaI 760 CTAG C^TAG
931 CTAG
BglI 23 GCCGGGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
125 GCCGAGAGGGC
BpmI 611 CTGGAG CTGGAG(16/14)
678 CTGGAG
BpuAI 1096 GAAGAC GAAGAC(2/6)
1291 GAAGAC
BsmAI 674 GAGAC GTCTC(1/5)
774 GTCTC
BspMI 780 ACCTGC ACCTGC(4/8)
1056 ACCTGC
BsrBI 135 CCGCTC CCGCTC(-3/-3)
448 GAGCGG
BstYI 823 GGATCC [GA]^GATC[CT]
1277 AGATCT
EarI 690 GAAGAG CTCTTC(1/4)
709 GAAGAG
HapII 24 CCGG C^CGG
720 CCGG
HinfI 471 GAATC G^A[ACGT]TC
603 GAATC
HpaII 24 CCGG C^CGG
720 CCGG
Ksp632I 690 GAAGAG CTCTTC(1/4)
709 GAAGAG
MaeI 760 CTAG C^TAG
931 CTAG
MaeIII 386 GTAAC ^GT[ACGT]AC
1156 GTGAC
MflI 823 GGATCC [GA]^GATC[CT]
1277 AGATCT
MspI 24 CCGG C^CGG
720 CCGG
NciI 24 CCGGG CC^[GC]GG
719 CCCGG
SexAI 353 ACCTGGT A^CC[AT]GGT
1242 ACCAGGT
TfiI 471 GAATC G^A[AT]TC
603 GAATC
XhoII 823 GGATCC [GA]^GATC[CT]
1277 AGATCT
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# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BanI BmyI BsaJI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DraII EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII ItaI MboI MboII
MnlI MslI MvaI MwoI NdeII
NlaIII NlaIV NspI RsaI Sau3AI
Sau96I ScrFI SfaNI Tsp509I TspRI