(Circular) (Six-base) MAPSORT of: pFN21AB8175.seq Check: 4701 from: 1 to: 6208 pFN21AB8175 6208 bp With 182 enzymes: SgfI * February 27, 2009 14:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2067 4018 278 Size: 53 83 186 936 531 1951 2468 Fragments arranged by size: 2468 1951 936 531 186 83 53 AccI GT'mk_AC Cuts at: 1937 3551 1937 Size: 1614 4594 Acc65I G'GTAC_C Cuts at: 3529 3529 Size: 6208 AclI AA'CG_TT Cuts at: 1563 4335 4708 1563 Size: 2772 373 3063 Fragments arranged by size: 3063 2772 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2366 2480 2567 3390 4271 5655 5923 1632 Size: 734 114 87 823 881 1384 268 1917 Fragments arranged by size: 1917 1384 881 823 734 268 114 87 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6011 AflIII A'CryG_T Cuts at: 1203 5107 6084 1203 Size: 3904 977 1327 Fragments arranged by size: 3904 1327 977 AgeI A'CCGG_T Cuts at: 5039 5039 Size: 6208 AhdI GACnn_n'nnGTC Cuts at: 4937 4937 Size: 6208 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6208 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6208 AlwNI CAG_nnn'CTG Cuts at: 3951 5523 3951 Size: 1572 4636 ApaI G_GGCC'C Cuts at: 3261 3261 Size: 6208 ApaLI G'TGCA_C Cuts at: 4265 5421 4265 Size: 1156 5052 ApoI r'AATT_y Cuts at: 1493 2252 2282 2330 3517 3743 3895 6004 1493 Size: 759 30 48 1187 226 152 2109 1697 Fragments arranged by size: 2109 1697 1187 759 226 152 48 30 AseI AT'TA_AT Cuts at: 160 1032 4762 160 Size: 872 3730 1606 Fragments arranged by size: 3730 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6208 AvaI C'yCGr_G Cuts at: 1870 1943 2133 2352 3005 3533 1870 Size: 73 190 219 653 528 4545 Fragments arranged by size: 4545 653 528 219 190 73 BamHI G'GATC_C Cuts at: 3538 3905 3538 Size: 367 5841 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2930 3529 4984 618 Size: 325 217 627 1143 599 1455 1842 Fragments arranged by size: 1842 1455 1143 627 599 325 217 BanII G_rGCy'C Cuts at: 1391 2220 2731 2851 3261 3527 1391 Size: 829 511 120 410 266 4072 Fragments arranged by size: 4072 829 511 410 266 120 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3450 3479 928 Size: 971 1551 29 3657 Fragments arranged by size: 3657 1551 971 29 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4399 4433 1903 Size: 34 2462 34 3678 Fragments arranged by size: 3678 2462 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4100 5310 4100 Size: 1210 4998 BclI T'GATC_A Cuts at: 1548 2122 1548 Size: 574 5634 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4819 136 Size: 107 122 71 631 605 3147 1525 Fragments arranged by size: 3147 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2307 2982 6203 2307 Size: 675 3221 2312 Fragments arranged by size: 3221 2312 675 BlpI GC'TnA_GC Cuts at: 3625 3625 Size: 6208 Bme1580I G_kGCm'C Cuts at: 1790 2713 2933 3261 4269 5425 1790 Size: 923 220 328 1008 1156 2573 Fragments arranged by size: 2573 1156 1008 923 328 220 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6208 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3939 4897 442 Size: 416 53 83 399 24 2522 958 1753 Fragments arranged by size: 2522 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6208 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2039 2745 2903 4868 1239 Size: 800 706 158 1965 2579 Fragments arranged by size: 2579 1965 800 706 158 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2252 2429 2594 3433 4333 5198 5496 2252 Size: 177 165 839 900 865 298 2964 Fragments arranged by size: 2964 900 865 839 298 177 165 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4871 882 Size: 617 3372 2219 Fragments arranged by size: 3372 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3999 493 Size: 711 2795 2702 Fragments arranged by size: 2795 2702 711 BsaBI GATnn'nnATC Cuts at: 2794 6202 2794 Size: 3408 2800 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2064 3292 4015 4397 Size: 53 83 186 936 531 1228 723 382 Cuts at: 4397 5032 275 Size: 635 1451 Fragments arranged by size: 1451 1228 936 723 635 531 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2289 4641 5039 5313 5460 5871 2289 Size: 2352 398 274 147 411 2626 Fragments arranged by size: 2626 2352 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5983 Fragments arranged by size: 5983 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2057 2228 2743 2777 1186 Size: 871 171 515 34 4617 Fragments arranged by size: 4617 871 515 171 34 BseYI C'CCAG_C Cuts at: 3162 5411 5907 3162 Size: 2249 496 3463 Fragments arranged by size: 3463 2249 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5635 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3658 4419 5447 1540 Size: 275 186 1657 761 1028 2301 Fragments arranged by size: 2301 1657 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2220 4269 4354 5425 2220 Size: 2049 85 1071 3003 Fragments arranged by size: 3003 2049 1071 85 BsmI GAATG_Cn' Cuts at: 2545 2747 3719 3812 2545 Size: 202 972 93 4941 Fragments arranged by size: 4941 972 202 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2220 2713 2731 2851 2933 3261 3527 Size: 399 430 493 18 120 82 328 266 Cuts at: 3527 4269 4354 5425 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 493 430 399 328 266 120 85 82 18 BspEI T'CCGG_A Cuts at: 5871 5871 Size: 6208 BspHI T'CATG_A Cuts at: 2038 3028 4097 5827 2038 Size: 990 1069 1730 2419 Fragments arranged by size: 2419 1730 1069 990 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3159 3563 844 Size: 2315 404 3489 Fragments arranged by size: 3489 2315 404 BsrBI CCG'CTC Cuts at: 1394 1823 4095 1394 Size: 429 2272 3507 Fragments arranged by size: 3507 2272 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2961 4703 4877 65 Size: 1183 1713 1742 174 1396 Fragments arranged by size: 1742 1713 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4852 5039 1700 Size: 110 3042 187 2869 Fragments arranged by size: 3042 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3037 96 Size: 2941 3267 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6208 BssSI C'ACGA_G Cuts at: 4268 5280 4268 Size: 1012 5196 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6208 BstBI TT'CG_AA Cuts at: 5012 5012 Size: 6208 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6208 BstXI CCAn_nnnn'nTGG Cuts at: 3162 3162 Size: 6208 BstYI r'GATC_y Cuts at: 1972 2307 2982 3484 3538 3905 4290 4307 5759 Size: 335 675 502 54 367 385 17 1452 Cuts at: 5759 5845 5857 6203 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 675 502 385 367 346 335 86 54 17 12 Bsu36I CC'TnA_GG Cuts at: 2174 2174 Size: 6208 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4717 Fragments arranged by size: 4717 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5469 Fragments arranged by size: 5469 683 56 BtsI GCAGTG_nn' Cuts at: 2713 3330 3724 4518 4538 2713 Size: 617 394 794 20 4383 Fragments arranged by size: 4383 794 617 394 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6208 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2307 2342 529 Size: 35 1743 35 4395 Fragments arranged by size: 4395 1743 35 35 DraI TTT'AAA Cuts at: 3512 3512 Size: 6208 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5215 809 Size: 507 3899 1802 Fragments arranged by size: 3899 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2690 3655 4544 Size: 54 1425 50 275 18 860 965 889 Cuts at: 4544 6173 8 Size: 1629 43 Fragments arranged by size: 1629 1425 965 889 860 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3655 1537 Size: 275 1843 4090 Fragments arranged by size: 4090 1843 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2364 2397 2835 3060 3072 3081 3114 4138 Size: 1011 33 438 225 12 9 33 1024 Cuts at: 4138 1353 Size: 3423 Fragments arranged by size: 3423 1024 1011 438 225 33 33 12 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4795 5169 5315 387 Size: 1064 138 3206 374 146 1280 Fragments arranged by size: 3206 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2218 2218 Size: 6208 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2039 2366 2480 2567 2745 2903 3390 Size: 393 407 327 114 87 178 158 487 Cuts at: 3390 4271 4868 5655 5923 1239 Size: 881 597 787 268 1524 Fragments arranged by size: 1524 881 787 597 487 407 393 327 268 178 158 114 87 EcoO109I rG'GnC_Cy Cuts at: 2234 2676 2832 3257 2234 Size: 442 156 425 5185 Fragments arranged by size: 5185 442 425 156 EcoRI G'AATT_C Cuts at: 2252 2282 3517 3895 2252 Size: 30 1235 378 4565 Fragments arranged by size: 4565 1235 378 30 FspI TGC'GCA Cuts at: 4714 4714 Size: 6208 HaeII r_GCGC'y Cuts at: 1678 2035 5355 1678 Size: 357 3320 2531 Fragments arranged by size: 3320 2531 357 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3356 3388 4849 4881 4923 Size: 32 135 32 1784 32 1461 32 42 Cuts at: 4923 4955 1373 Size: 32 2626 Fragments arranged by size: 2626 1784 1461 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3552 3798 669 Size: 988 281 1614 246 3079 Fragments arranged by size: 3079 1614 988 281 246 HindIII A'AGCT_T Cuts at: 748 3197 748 Size: 2449 3759 HpaI GTT'AAC Cuts at: 3798 3798 Size: 6208 KpnI G_GTAC'C Cuts at: 3533 3533 Size: 6208 MfeI C'AATT_G Cuts at: 3807 3807 Size: 6208 MluI A'CGCG_T Cuts at: 6084 6084 Size: 6208 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5322 5506 1305 Size: 4017 184 2007 Fragments arranged by size: 4017 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4386 Fragments arranged by size: 4386 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2301 3033 3300 4166 4525 4684 518 Size: 648 1135 732 267 866 359 159 2042 Fragments arranged by size: 2042 1135 866 732 648 359 267 159 MspA1I CmG'CkG Cuts at: 1606 2029 3624 4301 5449 5694 1606 Size: 423 1595 677 1148 245 2120 Fragments arranged by size: 2120 1595 1148 677 423 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4717 Fragments arranged by size: 4717 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6208 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6208 NotI GC'GGCC_GC Cuts at: 3655 3655 Size: 6208 NspI r_CATG'y Cuts at: 1132 1229 3566 5111 1132 Size: 97 2337 1545 2229 Fragments arranged by size: 2337 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6208 PciI A'CATG_T Cuts at: 5107 5107 Size: 6208 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6208 PfoI T'CCnGG_A Cuts at: 2103 2683 2103 Size: 580 5628 PmeI GTTT'AAAC Cuts at: 3512 3512 Size: 6208 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4277 4309 5790 5822 4277 Size: 32 1481 32 4663 Fragments arranged by size: 4663 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2234 2676 2832 2234 Size: 442 156 5610 Fragments arranged by size: 5610 442 156 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6208 PsiI TTA'TAA Cuts at: 3778 3778 Size: 6208 PspOMI G'GGCC_C Cuts at: 3257 3257 Size: 6208 PstI C_TGCA'G Cuts at: 830 3560 830 Size: 2730 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6208 SacI G_AGCT'C Cuts at: 2220 2220 Size: 6208 SalI G'TCGA_C Cuts at: 1936 3550 1936 Size: 1614 4594 SanDI GG'GwC_CC Cuts at: 2832 2832 Size: 6208 SapI GCTCTTCn'nnn_ Cuts at: 2835 3060 3081 2835 Size: 225 21 5962 Fragments arranged by size: 5962 225 21 SbfI CC_TGCA'GG Cuts at: 3560 3560 Size: 6208 ScaI AGT'ACT Cuts at: 4456 6168 4456 Size: 1712 4496 SfcI C'TryA_G Cuts at: 826 1045 3556 4691 5372 5563 826 Size: 219 2511 1135 681 191 1471 Fragments arranged by size: 2511 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6208 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6208 SmaI CCC'GGG Cuts at: 1872 3535 1872 Size: 1663 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2267 2352 2444 2573 3448 4312 Size: 197 926 324 85 92 129 875 864 Cuts at: 4312 5213 5475 820 Size: 901 262 1553 Fragments arranged by size: 1553 926 901 875 864 324 262 197 129 92 85 SnaBI TAC'GTA Cuts at: 493 3999 493 Size: 3506 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6208 SphI G_CATG'C Cuts at: 1132 3566 1132 Size: 2434 3774 SspI AAT'ATT Cuts at: 5 52 4132 5 Size: 47 4080 2081 Fragments arranged by size: 4080 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6208 StyI C'CwwG_G Cuts at: 513 1065 2004 3647 513 Size: 552 939 1643 3074 Fragments arranged by size: 3074 1643 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2313 4235 4252 4405 4590 5035 325 Size: 1255 733 1922 17 153 185 445 1498 Fragments arranged by size: 1922 1498 1255 733 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2448 3037 4454 Size: 274 80 33 51 1443 471 589 1417 Cuts at: 4454 6166 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1417 589 471 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6168 6200 6168 Size: 32 6176 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6208 XbaI T'CTAG_A Cuts at: 2971 3544 2971 Size: 573 5635 XhoI C'TCGA_G Cuts at: 1943 2352 1943 Size: 409 5799 XmaI C'CCGG_G Cuts at: 1870 3533 1870 Size: 1663 4545 XmnI GAAnn'nnTTC Cuts at: 4337 4337 Size: 6208 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2065 4016 276 Size: 53 83 186 936 531 1951 2468 Fragments arranged by size: 2468 1951 936 531 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacII SexAI SfiI SfoI SrfI SwaI XcmI