(Circular) (Six-base) MAPSORT of: pFN21AB8174.seq Check: 5201 from: 1 to: 6394 pFN21AB8174 6394 bp With 182 enzymes: SgfI * February 27, 2009 14:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4204 278 Size: 53 83 186 936 2668 2468 Fragments arranged by size: 2668 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3737 1937 Size: 1800 4594 Acc65I G'GTAC_C Cuts at: 2050 3715 2050 Size: 1665 4729 AclI AA'CG_TT Cuts at: 1563 4521 4894 1563 Size: 2958 373 3063 Fragments arranged by size: 3063 2958 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2079 2103 2420 3591 4457 5841 6109 1632 Size: 447 24 317 1171 866 1384 268 1917 Fragments arranged by size: 1917 1384 1171 866 447 317 268 24 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6197 AflIII A'CryG_T Cuts at: 1203 5293 6270 1203 Size: 4090 977 1327 Fragments arranged by size: 4090 1327 977 AgeI A'CCGG_T Cuts at: 5225 5225 Size: 6394 AhdI GACnn_n'nnGTC Cuts at: 5123 5123 Size: 6394 AleI CACnn'nnGTG Cuts at: 1166 3209 1166 Size: 2043 4351 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6394 AlwNI CAG_nnn'CTG Cuts at: 2876 3569 4137 5709 2876 Size: 693 568 1572 3561 Fragments arranged by size: 3561 1572 693 568 ApaLI G'TGCA_C Cuts at: 4451 5607 4451 Size: 1156 5238 ApoI r'AATT_y Cuts at: 1493 2519 2727 3703 3929 4081 6190 1493 Size: 1026 208 976 226 152 2109 1697 Fragments arranged by size: 2109 1697 1026 976 226 208 152 AseI AT'TA_AT Cuts at: 160 1032 4948 160 Size: 872 3916 1606 Fragments arranged by size: 3916 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6394 AvaI C'yCGr_G Cuts at: 1870 1943 3719 1870 Size: 73 1776 4545 Fragments arranged by size: 4545 1776 73 BamHI G'GATC_C Cuts at: 3724 4091 3724 Size: 367 6027 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2050 3715 5170 618 Size: 325 217 627 263 1665 1455 1842 Fragments arranged by size: 1842 1665 1455 627 325 263 217 BanII G_rGCy'C Cuts at: 1391 2551 3152 3266 3401 3713 1391 Size: 1160 601 114 135 312 4072 Fragments arranged by size: 4072 1160 601 312 135 114 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2091 928 Size: 971 192 5231 Fragments arranged by size: 5231 971 192 BbvCI CC'TCA_GC Cuts at: 3254 3303 3611 3254 Size: 49 308 6037 Fragments arranged by size: 6037 308 49 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4585 4619 1903 Size: 34 2648 34 3678 Fragments arranged by size: 3678 2648 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4286 5496 4286 Size: 1210 5184 BclI T'GATC_A Cuts at: 1548 2347 2355 2532 1548 Size: 799 8 177 5410 Fragments arranged by size: 5410 799 177 8 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5005 136 Size: 107 122 71 631 605 3333 1525 Fragments arranged by size: 3333 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6389 6389 Size: 6394 BlpI GC'TnA_GC Cuts at: 3395 3811 3395 Size: 416 5978 Bme1580I G_kGCm'C Cuts at: 1790 4455 5611 1790 Size: 2665 1156 2573 Fragments arranged by size: 2665 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6394 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2024 3203 3437 Size: 416 53 83 399 24 607 1179 234 Cuts at: 3437 4125 5083 442 Size: 688 958 1753 Fragments arranged by size: 1753 1179 958 688 607 416 399 234 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6394 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3171 3171 Size: 6394 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2535 3104 3250 5054 1239 Size: 1296 569 146 1804 2579 Fragments arranged by size: 2579 1804 1296 569 146 Bpu10I CC'TnA_GC Cuts at: 2441 3096 3254 3303 3611 2441 Size: 655 158 49 308 5224 Fragments arranged by size: 5224 655 308 158 49 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3166 4519 5384 5682 3166 Size: 1353 865 298 3878 Fragments arranged by size: 3878 1353 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3334 5057 882 Size: 617 1835 1723 2219 Fragments arranged by size: 2219 1835 1723 617 BsaAI yAC'GTr Cuts at: 493 1204 4185 493 Size: 711 2981 2702 Fragments arranged by size: 2981 2702 711 BsaBI GATnn'nnATC Cuts at: 2621 6388 2621 Size: 3767 2627 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4201 4583 5218 275 Size: 53 83 186 936 2668 382 635 1451 Fragments arranged by size: 2668 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2459 4827 5225 5499 5646 6057 2459 Size: 2368 398 274 147 411 2796 Fragments arranged by size: 2796 2368 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6169 Fragments arranged by size: 6169 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2282 2714 2831 3029 3147 3322 3635 1186 Size: 1096 432 117 198 118 175 313 3945 Fragments arranged by size: 3945 1096 432 313 198 175 118 117 BseYI C'CCAG_C Cuts at: 2144 5597 6093 2144 Size: 3453 496 2445 Fragments arranged by size: 3453 2445 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2600 1163 Size: 573 864 4957 Fragments arranged by size: 4957 864 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3844 4605 5633 1540 Size: 275 186 1843 761 1028 2301 Fragments arranged by size: 2301 1843 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2551 3092 3401 4455 4540 5611 2551 Size: 541 309 1054 85 1071 3334 Fragments arranged by size: 3334 1071 1054 541 309 85 BsmI GAATG_Cn' Cuts at: 3905 3998 3905 Size: 93 6301 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2551 3092 3152 3266 3401 3713 4455 Size: 399 761 541 60 114 135 312 742 Cuts at: 4455 4540 5611 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 761 742 541 399 312 135 114 85 60 BspEI T'CCGG_A Cuts at: 2459 6057 2459 Size: 3598 2796 BspHI T'CATG_A Cuts at: 4283 6013 4283 Size: 1730 4664 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2513 3749 844 Size: 1669 1236 3489 Fragments arranged by size: 3489 1669 1236 BsrBI CCG'CTC Cuts at: 1394 1823 4281 1394 Size: 429 2458 3507 Fragments arranged by size: 3507 2458 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4889 5063 65 Size: 1183 3641 174 1396 Fragments arranged by size: 3641 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5038 5225 1700 Size: 110 3228 187 2869 Fragments arranged by size: 3228 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6394 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6394 BssSI C'ACGA_G Cuts at: 4454 5466 4454 Size: 1012 5382 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6394 BstBI TT'CG_AA Cuts at: 5198 5198 Size: 6394 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6394 BstYI r'GATC_y Cuts at: 1972 3724 4091 4476 4493 5945 6031 6043 6389 Size: 1752 367 385 17 1452 86 12 346 Cuts at: 6389 1972 Size: 1977 Fragments arranged by size: 1977 1752 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2688 2694 3228 3444 3471 513 Size: 552 939 684 6 534 216 27 3436 Fragments arranged by size: 3436 939 684 552 534 216 27 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5655 Fragments arranged by size: 5655 683 56 BtsI GCAGTG_nn' Cuts at: 2545 3287 3910 4704 4724 2545 Size: 742 623 794 20 4215 Fragments arranged by size: 4215 794 742 623 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6394 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6359 DraI TTT'AAA Cuts at: 3698 3698 Size: 6394 DraIII CAC_nnn'GTG Cuts at: 3670 3670 Size: 6394 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5401 809 Size: 507 4085 1802 Fragments arranged by size: 4085 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2691 3125 3246 Size: 54 1425 50 275 18 861 434 121 Cuts at: 3246 3468 3841 4730 6359 8 Size: 222 373 889 1629 43 Fragments arranged by size: 1629 1425 889 861 434 373 275 222 121 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3841 1537 Size: 275 2029 4090 Fragments arranged by size: 4090 2029 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2405 2625 2637 2778 3314 4324 1353 Size: 1052 220 12 141 536 1010 3423 Fragments arranged by size: 3423 1052 1010 536 220 141 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4981 5355 5501 387 Size: 1064 138 3392 374 146 1280 Fragments arranged by size: 3392 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2549 3399 2549 Size: 850 5544 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2079 2103 2420 2535 3104 3250 3591 Size: 393 447 24 317 115 569 146 341 Cuts at: 3591 4457 5054 5841 6109 1239 Size: 866 597 787 268 1524 Fragments arranged by size: 1524 866 787 597 569 447 393 341 317 268 146 115 24 EcoNI CCTnn'n_nnAGG Cuts at: 2058 3257 2058 Size: 1199 5195 EcoO109I rG'GnC_Cy Cuts at: 3111 3111 Size: 6394 EcoRI G'AATT_C Cuts at: 3703 4081 3703 Size: 378 6016 FspI TGC'GCA Cuts at: 4900 4900 Size: 6394 HaeII r_GCGC'y Cuts at: 1678 3392 5541 1678 Size: 1714 2149 2531 Fragments arranged by size: 2531 2149 1714 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5035 5067 5109 5141 1373 Size: 32 135 32 3463 32 42 32 2626 Fragments arranged by size: 3463 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3738 3984 669 Size: 988 281 1800 246 3079 Fragments arranged by size: 3079 1800 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6394 HpaI GTT'AAC Cuts at: 3984 3984 Size: 6394 KpnI G_GTAC'C Cuts at: 2054 3719 2054 Size: 1665 4729 MfeI C'AATT_G Cuts at: 3993 3993 Size: 6394 MluI A'CGCG_T Cuts at: 6270 6270 Size: 6394 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2130 2991 5508 5692 1305 Size: 825 861 2517 184 2007 Fragments arranged by size: 2517 2007 861 825 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2693 3248 3470 10 Size: 54 1425 343 861 555 222 2934 Fragments arranged by size: 2934 1425 861 555 343 222 54 MslI CAynn'nnrTG Cuts at: 518 1166 2744 3209 4352 4711 4870 518 Size: 648 1578 465 1143 359 159 2042 Fragments arranged by size: 2042 1578 1143 648 465 359 159 MspA1I CmG'CkG Cuts at: 1606 2678 2842 3124 3810 4487 5635 5880 1606 Size: 1072 164 282 686 677 1148 245 2120 Fragments arranged by size: 2120 1148 1072 686 677 282 245 164 NcoI C'CATG_G Cuts at: 513 1065 2004 2688 3228 3444 3471 513 Size: 552 939 684 540 216 27 3436 Fragments arranged by size: 3436 939 684 552 540 216 27 NdeI CA'TA_TG Cuts at: 387 387 Size: 6394 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6394 NotI GC'GGCC_GC Cuts at: 3841 3841 Size: 6394 NspI r_CATG'y Cuts at: 1132 1229 3752 5297 1132 Size: 97 2523 1545 2229 Fragments arranged by size: 2523 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6394 PciI A'CATG_T Cuts at: 5293 5293 Size: 6394 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6394 PfoI T'CCnGG_A Cuts at: 2314 2314 Size: 6394 PmeI GTTT'AAAC Cuts at: 3698 3698 Size: 6394 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2964 2996 4463 4495 5976 6008 2964 Size: 32 1467 32 1481 32 3350 Fragments arranged by size: 3350 1481 1467 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6394 PsiI TTA'TAA Cuts at: 3964 3964 Size: 6394 PstI C_TGCA'G Cuts at: 830 2510 3746 830 Size: 1680 1236 3478 Fragments arranged by size: 3478 1680 1236 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6394 SacI G_AGCT'C Cuts at: 2551 3401 2551 Size: 850 5544 SalI G'TCGA_C Cuts at: 1936 3736 1936 Size: 1800 4594 SapI GCTCTTCn'nnn_ Cuts at: 3314 3314 Size: 6394 SbfI CC_TGCA'GG Cuts at: 3746 3746 Size: 6394 ScaI AGT'ACT Cuts at: 4642 6354 4642 Size: 1712 4682 SfcI C'TryA_G Cuts at: 826 1045 2226 2506 3742 4877 5558 5749 826 Size: 219 1181 280 1236 1135 681 191 1471 Fragments arranged by size: 1471 1236 1181 1135 681 280 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6394 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6394 SmaI CCC'GGG Cuts at: 1872 3721 1872 Size: 1849 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3145 4498 5399 5661 820 Size: 197 926 1202 1353 901 262 1553 Fragments arranged by size: 1553 1353 1202 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4185 493 Size: 3692 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6394 SphI G_CATG'C Cuts at: 1132 3752 1132 Size: 2620 3774 SspI AAT'ATT Cuts at: 5 52 3238 4318 5 Size: 47 3186 1080 2081 Fragments arranged by size: 3186 2081 1080 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6394 StyI C'CwwG_G Cuts at: 513 1065 2004 2688 3228 3444 3471 3833 513 Size: 552 939 684 540 216 27 362 3074 Fragments arranged by size: 3074 939 684 552 540 362 216 27 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2417 2602 3208 4421 4438 4591 4776 Size: 1255 837 185 606 1213 17 153 185 Cuts at: 4776 5221 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1213 837 606 445 185 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3422 4640 6352 Size: 274 80 33 51 1443 1445 1218 1712 Cuts at: 6352 96 Size: 138 Fragments arranged by size: 1712 1445 1443 1218 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2310 2342 6354 6386 2310 Size: 32 4012 32 2318 Fragments arranged by size: 4012 2318 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2921 1316 Size: 1605 4789 XbaI T'CTAG_A Cuts at: 3730 3730 Size: 6394 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3208 3208 Size: 6394 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6394 XmaI C'CCGG_G Cuts at: 1870 3719 1870 Size: 1849 4545 XmnI GAAnn'nnTTC Cuts at: 2976 4523 2976 Size: 1547 4847 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4202 276 Size: 53 83 186 936 2668 2468 Fragments arranged by size: 2668 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI