(Circular) (Six-base) MAPSORT of: pFN21AB8170.seq Check: 4754 from: 1 to: 5812 pFN21AB8170 5812 bp With 182 enzymes: SgfI * February 27, 2009 14:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3622 278 Size: 53 83 186 936 2086 2468 Fragments arranged by size: 2468 2086 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3155 1937 Size: 1218 4594 Acc65I G'GTAC_C Cuts at: 3133 3133 Size: 5812 AclI AA'CG_TT Cuts at: 1563 3939 4312 1563 Size: 2376 373 3063 Fragments arranged by size: 3063 2376 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2084 2276 2366 2457 2555 2747 2905 3083 Size: 452 192 90 91 98 192 158 178 Cuts at: 3083 3875 5259 5527 1632 Size: 792 1384 268 1917 Fragments arranged by size: 1917 1384 792 452 268 192 192 178 158 98 91 90 AfeI AGC'GCT Cuts at: 2600 2600 Size: 5812 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5615 AflIII A'CryG_T Cuts at: 1203 2856 4711 5688 1203 Size: 1653 1855 977 1327 Fragments arranged by size: 1855 1653 1327 977 AgeI A'CCGG_T Cuts at: 4643 4643 Size: 5812 AhdI GACnn_n'nnGTC Cuts at: 2047 4541 2047 Size: 2494 3318 AleI CACnn'nnGTG Cuts at: 1166 2087 1166 Size: 921 4891 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5812 AlwNI CAG_nnn'CTG Cuts at: 2320 2466 2654 2806 2879 3555 5127 2320 Size: 146 188 152 73 676 1572 3005 Fragments arranged by size: 3005 1572 676 188 152 146 73 ApaI G_GGCC'C Cuts at: 2351 2608 2644 2351 Size: 257 36 5519 Fragments arranged by size: 5519 257 36 ApaLI G'TGCA_C Cuts at: 3869 5025 3869 Size: 1156 4656 ApoI r'AATT_y Cuts at: 1493 2405 3121 3347 3499 5608 1493 Size: 912 716 226 152 2109 1697 Fragments arranged by size: 2109 1697 912 716 226 152 AseI AT'TA_AT Cuts at: 160 1032 4366 160 Size: 872 3334 1606 Fragments arranged by size: 3334 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5812 AvaI C'yCGr_G Cuts at: 1870 1943 2643 3137 1870 Size: 73 700 494 4545 Fragments arranged by size: 4545 700 494 73 BamHI G'GATC_C Cuts at: 2436 3142 3509 2436 Size: 706 367 4739 Fragments arranged by size: 4739 706 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2181 2777 3133 4588 618 Size: 325 217 627 394 596 356 1455 1842 Fragments arranged by size: 1842 1455 627 596 394 356 325 217 BanII G_rGCy'C Cuts at: 1391 2287 2351 2484 2608 2644 3131 1391 Size: 896 64 133 124 36 487 4072 Fragments arranged by size: 4072 896 487 133 124 64 36 BbeI G_GCGC'C Cuts at: 2185 2185 Size: 5812 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2160 928 Size: 971 261 4580 Fragments arranged by size: 4580 971 261 BbvCI CC'TCA_GC Cuts at: 3107 3107 Size: 5812 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2104 2138 4003 4037 1903 Size: 34 167 34 1865 34 3678 Fragments arranged by size: 3678 1865 167 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3704 4914 3704 Size: 1210 4602 BclI T'GATC_A Cuts at: 1548 1548 Size: 5812 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4423 136 Size: 107 122 71 631 605 2751 1525 Fragments arranged by size: 2751 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5807 5807 Size: 5812 BlpI GC'TnA_GC Cuts at: 2556 3036 3057 3229 2556 Size: 480 21 172 5139 Fragments arranged by size: 5139 480 172 21 Bme1580I G_kGCm'C Cuts at: 1790 2351 2608 2644 2782 3079 3873 5029 1790 Size: 561 257 36 138 297 794 1156 2573 Fragments arranged by size: 2573 1156 794 561 297 257 138 36 BmgBI CAC'GTC Cuts at: 1318 2428 1318 Size: 1110 4702 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3543 4501 442 Size: 416 53 83 399 24 2126 958 1753 Fragments arranged by size: 2126 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5812 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2168 2174 2642 2966 4472 1239 Size: 929 6 468 324 1506 2579 Fragments arranged by size: 2579 1506 929 468 324 6 Bpu10I CC'TnA_GC Cuts at: 3107 3107 Size: 5812 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2216 2543 3937 4802 5100 2216 Size: 327 1394 865 298 2928 Fragments arranged by size: 2928 1394 865 327 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2150 2262 4475 882 Size: 617 651 112 2213 2219 Fragments arranged by size: 2219 2213 651 617 112 BsaAI yAC'GTr Cuts at: 493 1204 3603 493 Size: 711 2399 2702 Fragments arranged by size: 2702 2399 711 BsaBI GATnn'nnATC Cuts at: 5806 5806 Size: 5812 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2182 3619 4001 4636 Size: 53 83 186 936 649 1437 382 635 Cuts at: 4636 275 Size: 1451 Fragments arranged by size: 1451 1437 936 649 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4245 4643 4917 5064 5475 4245 Size: 398 274 147 411 4582 Fragments arranged by size: 4582 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5587 Fragments arranged by size: 5587 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2141 2522 2540 2639 2978 1186 Size: 955 381 18 99 339 4020 Fragments arranged by size: 4020 955 381 339 99 18 BseYI C'CCAG_C Cuts at: 2260 2595 2601 2742 5015 5511 2260 Size: 335 6 141 2273 496 2561 Fragments arranged by size: 2561 2273 496 335 141 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5239 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3262 4023 5051 1540 Size: 275 186 1261 761 1028 2301 Fragments arranged by size: 2301 1261 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2287 3873 3958 5029 2287 Size: 1586 85 1071 3070 Fragments arranged by size: 3070 1586 1071 85 BsmI GAATG_Cn' Cuts at: 3323 3416 3323 Size: 93 5719 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2287 2351 2484 2608 2644 2782 3079 Size: 399 497 64 133 124 36 138 297 Cuts at: 3079 3131 3873 3958 5029 1391 Size: 52 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 497 399 297 138 133 124 85 64 52 36 BspEI T'CCGG_A Cuts at: 5475 5475 Size: 5812 BspHI T'CATG_A Cuts at: 3701 5431 3701 Size: 1730 4082 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2625 3111 3167 844 Size: 1781 486 56 3489 Fragments arranged by size: 3489 1781 486 56 BsrBI CCG'CTC Cuts at: 1394 1823 3699 1394 Size: 429 1876 3507 Fragments arranged by size: 3507 1876 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4307 4481 65 Size: 1183 3059 174 1396 Fragments arranged by size: 3059 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2178 4456 4643 1700 Size: 110 368 2278 187 2869 Fragments arranged by size: 2869 2278 368 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5812 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5812 BssSI C'ACGA_G Cuts at: 3872 4884 3872 Size: 1012 4800 BstAPI GCAn_nnn'nTGC Cuts at: 794 3074 3103 794 Size: 2280 29 3503 Fragments arranged by size: 3503 2280 29 BstBI TT'CG_AA Cuts at: 4616 4616 Size: 5812 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5812 BstXI CCAn_nnnn'nTGG Cuts at: 2279 2279 Size: 5812 BstYI r'GATC_y Cuts at: 1972 2211 2391 2436 2568 3142 3509 3894 3911 Size: 239 180 45 132 574 367 385 17 Cuts at: 3911 5363 5449 5461 5807 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 574 385 367 346 239 180 132 86 45 17 12 BtgI C'CryG_G Cuts at: 513 1065 2432 513 Size: 552 1367 3893 Fragments arranged by size: 3893 1367 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5073 Fragments arranged by size: 5073 683 56 BtsI GCAGTG_nn' Cuts at: 2094 3328 4122 4142 2094 Size: 1234 794 20 3764 Fragments arranged by size: 3764 1234 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5812 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5777 DraI TTT'AAA Cuts at: 3116 3116 Size: 5812 DraIII CAC_nnn'GTG Cuts at: 3074 3074 Size: 5812 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2164 4819 809 Size: 507 848 2655 1802 Fragments arranged by size: 2655 1802 848 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3259 4148 5777 Size: 54 1425 50 275 18 1429 889 1629 Cuts at: 5777 8 Size: 43 Fragments arranged by size: 1629 1429 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3259 1537 Size: 275 1447 4090 Fragments arranged by size: 4090 1447 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3742 1353 Size: 2389 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4399 4773 4919 387 Size: 1064 138 2810 374 146 1280 Fragments arranged by size: 2810 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2285 2285 Size: 5812 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2084 2168 2174 2276 2366 2457 2555 Size: 393 452 84 6 102 90 91 98 Cuts at: 2555 2642 2747 2905 2966 3083 3875 4472 5259 Size: 87 105 158 61 117 792 597 787 Cuts at: 5259 5527 1239 Size: 268 1524 Fragments arranged by size: 1524 792 787 597 452 393 268 158 117 105 102 98 91 90 87 84 61 6 EcoNI CCTnn'n_nnAGG Cuts at: 2355 2621 2798 2810 2355 Size: 266 177 12 5357 Fragments arranged by size: 5357 266 177 12 EcoO109I rG'GnC_Cy Cuts at: 2348 2614 2640 2803 2980 3028 2348 Size: 266 26 163 177 48 5132 Fragments arranged by size: 5132 266 177 163 48 26 EcoRI G'AATT_C Cuts at: 2405 3121 3499 2405 Size: 716 378 4718 Fragments arranged by size: 4718 716 378 FspI TGC'GCA Cuts at: 4318 4318 Size: 5812 HaeII r_GCGC'y Cuts at: 1678 2185 2602 2794 4959 1678 Size: 507 417 192 2165 2531 Fragments arranged by size: 2531 2165 507 417 192 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2033 2065 4453 4485 4527 Size: 32 135 32 461 32 2388 32 42 Cuts at: 4527 4559 1373 Size: 32 2626 Fragments arranged by size: 2626 2388 461 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3156 3402 669 Size: 988 281 1218 246 3079 Fragments arranged by size: 3079 1218 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5812 HpaI GTT'AAC Cuts at: 3402 3402 Size: 5812 KasI G'GCGC_C Cuts at: 2181 2181 Size: 5812 KpnI G_GTAC'C Cuts at: 3137 3137 Size: 5812 MfeI C'AATT_G Cuts at: 3411 3411 Size: 5812 MluI A'CGCG_T Cuts at: 5688 5688 Size: 5812 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4926 5110 1305 Size: 3621 184 2007 Fragments arranged by size: 3621 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3990 Fragments arranged by size: 3990 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2087 3770 4129 4288 518 Size: 648 921 1683 359 159 2042 Fragments arranged by size: 2042 1683 921 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2146 2651 3228 3905 5053 5298 1606 Size: 540 505 577 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 577 540 505 245 NaeI GCC'GGC Cuts at: 2180 2180 Size: 5812 NarI GG'CG_CC Cuts at: 2182 2182 Size: 5812 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5260 NdeI CA'TA_TG Cuts at: 387 387 Size: 5812 NgoMIV G'CCGG_C Cuts at: 2178 2178 Size: 5812 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5812 NotI GC'GGCC_GC Cuts at: 3259 3259 Size: 5812 NspI r_CATG'y Cuts at: 1132 1229 2860 3170 4715 1132 Size: 97 1631 310 1545 2229 Fragments arranged by size: 2229 1631 1545 310 97 PasI CC'CwG_GG Cuts at: 1348 2578 2977 1348 Size: 1230 399 4183 Fragments arranged by size: 4183 1230 399 PciI A'CATG_T Cuts at: 2856 4711 2856 Size: 1855 3957 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5812 PfoI T'CCnGG_A Cuts at: 2394 2394 Size: 5812 PmeI GTTT'AAAC Cuts at: 3116 3116 Size: 5812 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3881 3913 5394 5426 3881 Size: 32 1481 32 4267 Fragments arranged by size: 4267 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2614 3028 2614 Size: 414 5398 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5812 PsiI TTA'TAA Cuts at: 3382 3382 Size: 5812 PspOMI G'GGCC_C Cuts at: 2347 2604 2640 2347 Size: 257 36 5519 Fragments arranged by size: 5519 257 36 PstI C_TGCA'G Cuts at: 830 2145 2802 3164 830 Size: 1315 657 362 3478 Fragments arranged by size: 3478 1315 657 362 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5812 PvuII CAG'CTG Cuts at: 2146 2146 Size: 5812 SacI G_AGCT'C Cuts at: 2287 2287 Size: 5812 SalI G'TCGA_C Cuts at: 1936 3154 1936 Size: 1218 4594 SbfI CC_TGCA'GG Cuts at: 2802 3164 2802 Size: 362 5450 ScaI AGT'ACT Cuts at: 4060 5772 4060 Size: 1712 4100 SfcI C'TryA_G Cuts at: 826 1045 2141 2798 3160 4295 4976 5167 826 Size: 219 1096 657 362 1135 681 191 1471 Fragments arranged by size: 1471 1135 1096 681 657 362 219 191 SfoI GGC'GCC Cuts at: 2183 2183 Size: 5812 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5812 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5812 SmaI CCC'GGG Cuts at: 1872 2645 3139 1872 Size: 773 494 4545 Fragments arranged by size: 4545 773 494 SmlI C'TyrA_G Cuts at: 820 1017 1943 2195 2522 3916 4817 5079 820 Size: 197 926 252 327 1394 901 262 1553 Fragments arranged by size: 1553 1394 926 901 327 262 252 197 SnaBI TAC'GTA Cuts at: 493 3603 493 Size: 3110 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5812 SphI G_CATG'C Cuts at: 1132 3170 1132 Size: 2038 3774 SrfI GCCC'GGGC Cuts at: 2645 2645 Size: 5812 SspI AAT'ATT Cuts at: 5 52 2821 3736 5 Size: 47 2769 915 2081 Fragments arranged by size: 2769 2081 915 47 StuI AGG'CCT Cuts at: 1517 2676 1517 Size: 1159 4653 StyI C'CwwG_G Cuts at: 513 1065 3251 513 Size: 552 2186 3074 Fragments arranged by size: 3074 2186 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2708 3839 3856 4009 4194 4639 325 Size: 1255 1128 1131 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1131 1128 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4058 5770 96 Size: 274 80 33 51 1443 2081 1712 138 Fragments arranged by size: 2081 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5772 5804 5772 Size: 32 5780 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5812 XbaI T'CTAG_A Cuts at: 3148 3148 Size: 5812 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5812 XmaI C'CCGG_G Cuts at: 1870 2643 3137 1870 Size: 773 494 4545 Fragments arranged by size: 4545 773 494 XmnI GAAnn'nnTTC Cuts at: 3941 3941 Size: 5812 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3620 276 Size: 53 83 186 936 2086 2468 Fragments arranged by size: 2468 2086 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SwaI XcmI