Restriction map for
pFN21AB8169
##################################
# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI Alw44I AocI Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspI AviII AvrII BbeI BbvCI
BcgI BciVI BclI BcnI BfrI
BglII BlnI BsaBI BsaWI BseAI
BsiEI BsiWI BsmI BspDI BspEI
BspLU11I BsrBI BsrDI BsrFI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I CfoI Cfr10I ClaI
CpoI DraI EagI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII HapII HhaI HinP1I HincII
HindII HindIII HpaI HpaII KasI
KpnI KspI MamI MfeI MluI
MroI MspI MunI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmeI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacII SalI ScaI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI
StuI SwaI Tth111I Van91I XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AccI 853 GTCTAC GT^[AC][GT]AC
AccII 764 CGCG CG^CG
AcsI 25 AAATTT [GA]^AATT[CT]
AcyI 361 GACGCC G[GA]^CG[CT]C
AhdI 583 GACATCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 112 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 25 AAATTT [GA]^AATT[CT]
AspEI 583 GACATCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 486 CCCGAG C^[CT]CG[GA]G
BalI 879 TGGCCA TGG^CCA
BanI 941 GGTGCC G^G[CT][GA]CC
BbrPI 787 CACGTG CAC^GTG
BbsI 285 GAAGAC GAAGAC(2/6)
BlpI 637 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 637 GCTCAGC GC^T[ACGT]AGC
BpuAI 285 GAAGAC GAAGAC(2/6)
BsaAI 787 CACGTG [CT]AC^GT[GA]
BsaHI 361 GACGCC G[GA]^CG[CT]C
BsaI 39 GGTCTC GGTCTC(1/5)
BseRI 970 GAGGAG GAGGAG(10/8)
BsmBI 359 GAGACG CGTCTC(1/5)
BsoBI 486 CCCGAG C^[CT]CG[GA]G
Bsp1407I 713 TGTACA T^GTACA
BspHI 890 TCATGA T^CATGA
BsrGI 713 TGTACA T^GTACA
BstAPI 370 GCAGGAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 764 CGCG CG^CG
BstXI 135 CCAGGTCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 637 GCTCAGC GC^T[ACGT]AGC
DrdI 130 GACACCCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 141 CCATGG C^C[GA][CT]GG
EaeI 879 TGGCCA [CT]^GGCC[GA]
Eam1105I 583 GACATCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 555 CTCTTC CTCTTC(1/4)
Hin1I 361 GACGCC G[GA]^CG[CT]C
HinfI 340 GATTC G^A[ACGT]TC
Ksp632I 555 CTCTTC CTCTTC(1/4)
MaeII 788 ACGT A^CGT
MluNI 879 TGGCCA TGG^CCA
MscI 879 TGGCCA TGG^CCA
MseI 698 TTAA T^TAA
MslI 502 CACTTTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 276 CAGCGG C[AC]G^C[GT]G
MvnI 764 CGCG CG^CG
NcoI 141 CCATGG C^CATGG
PmaCI 787 CACGTG CAC^GTG
PmlI 787 CACGTG CAC^GTG
RcaI 890 TCATGA T^CATGA
SapI 554 GCTCTTC GCTCTTC(1/4)
SbfI 367 CCTGCAGG CCTGCA^GG
SfaNI 376 GATGC GCATC(5/9)
Sse8387I 367 CCTGCAGG CCTGCA^GG
SspBI 713 TGTACA T^GTACA
TaqI 606 TCGA T^CGA
TfiI 340 GATTC G^A[AT]TC
Tru9I 698 TTAA T^TAA
TthHB8I 606 TCGA T^CGA
XbaI 171 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AfaI 714 GTAC GT^AC
803 GTAC
ApaI 187 GGGCCC GGGCC^C
907 GGGCCC
Asp700I 150 GAAATTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
429 GAACGATTTC
AspHI 316 GAGCTC G[AT]GC[AT]^C
552 GAGCTC
BamHI 298 GGATCC G^GATCC
725 GGATCC
BsgI 499 CTGCAC GTGCAG(16/14)
232 GTGCAG
BsiHKAI 316 GAGCTC G[AT]GC[AT]^C
552 GAGCTC
BspMI 60 GCAGGT ACCTGC(4/8)
261 GCAGGT
BstYI 298 GGATCC [GA]^GATC[CT]
725 GGATCC
DraIII 787 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
933 CACCTGGTG
EcoT14I 141 CCATGG C^C[AT][AT]GG
828 CCAAGG
HgaI 361 GACGC GACGC(5/10)
765 GCGTC
MflI 298 GGATCC [GA]^GATC[CT]
725 GGATCC
NlaIII 142 CATG CATG^
891 CATG
PpuMI 476 GGGACCC [GA]G^G[AT]CC[CT]
773 AGGTCCT
RsaI 714 GTAC GT^AC
803 GTAC
SacI 316 GAGCTC GAGCT^C
552 GAGCTC
SexAI 884 ACCAGGT A^CC[AT]GGT
934 ACCTGGT
StyI 141 CCATGG C^C[AT][AT]GG
828 CCAAGG
Tsp45I 568 GTCAC ^GT[GC]AC
581 GTGAC
XhoII 298 GGATCC [GA]^GATC[CT]
725 GGATCC
XmnI 150 GAAATTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
429 GAACGATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AluI AlwI AvaII BanII
BbvI BfaI BglI BmyI BpmI
BsaJI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII DraII
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HphI ItaI MaeI MaeIII
MboI MboII MnlI MvaI MwoI
NdeII NlaIV PstI Sau3AI Sau96I
ScrFI SfcI TseI Tsp509I TspRI