(Circular) (Six-base) MAPSORT of: pFN21AB8169.seq Check: 9734 from: 1 to: 5701 pFN21AB8169 5701 bp With 182 enzymes: SgfI * February 27, 2009 14:40 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2056 2056 Size: 5701 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3511 278 Size: 53 83 186 936 1975 2468 Fragments arranged by size: 2468 1975 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2859 3044 1937 Size: 922 185 4594 Fragments arranged by size: 4594 922 185 Acc65I G'GTAC_C Cuts at: 3022 3022 Size: 5701 AclI AA'CG_TT Cuts at: 1563 3828 4201 1563 Size: 2265 373 3063 Fragments arranged by size: 3063 2265 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3764 5148 5416 1632 Size: 2132 1384 268 1917 Fragments arranged by size: 2132 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5504 AflIII A'CryG_T Cuts at: 1203 4600 5577 1203 Size: 3397 977 1327 Fragments arranged by size: 3397 1327 977 AgeI A'CCGG_T Cuts at: 4532 4532 Size: 5701 AhdI GACnn_n'nnGTC Cuts at: 2593 4430 2593 Size: 1837 3864 AleI CACnn'nnGTG Cuts at: 1166 2511 1166 Size: 1345 4356 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5701 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2426 2458 2426 Size: 32 5669 AlwNI CAG_nnn'CTG Cuts at: 2122 3444 5016 2122 Size: 1322 1572 2807 Fragments arranged by size: 2807 1572 1322 ApaI G_GGCC'C Cuts at: 2196 2916 2196 Size: 720 4981 ApaLI G'TGCA_C Cuts at: 3758 4914 3758 Size: 1156 4545 ApoI r'AATT_y Cuts at: 1493 2030 3010 3236 3388 5497 1493 Size: 537 980 226 152 2109 1697 Fragments arranged by size: 2109 1697 980 537 226 152 AseI AT'TA_AT Cuts at: 160 1032 4255 160 Size: 872 3223 1606 Fragments arranged by size: 3223 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5701 AvaI C'yCGr_G Cuts at: 1870 1943 2491 3026 1870 Size: 73 548 535 4545 Fragments arranged by size: 4545 548 535 73 BamHI G'GATC_C Cuts at: 2303 2730 3031 3398 2303 Size: 427 301 367 4606 Fragments arranged by size: 4606 427 367 301 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2946 3022 4477 618 Size: 325 217 627 1159 76 1455 1842 Fragments arranged by size: 1842 1455 1159 627 325 217 76 BanII G_rGCy'C Cuts at: 1391 2196 2280 2325 2561 2916 3020 1391 Size: 805 84 45 236 355 104 4072 Fragments arranged by size: 4072 805 355 236 104 84 45 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2297 928 Size: 971 398 4332 Fragments arranged by size: 4332 971 398 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3892 3926 1903 Size: 34 50 34 1871 34 3678 Fragments arranged by size: 3678 1871 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3593 4803 3593 Size: 1210 4491 BclI T'GATC_A Cuts at: 1548 1548 Size: 5701 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2014 2284 2883 Size: 107 122 71 631 605 342 270 599 Cuts at: 2883 4312 136 Size: 1429 1525 Fragments arranged by size: 1525 1429 631 605 599 342 270 122 107 71 BglII A'GATC_T Cuts at: 5696 5696 Size: 5701 BlpI GC'TnA_GC Cuts at: 2643 3118 2643 Size: 475 5226 Bme1580I G_kGCm'C Cuts at: 1790 2196 2879 2916 2951 3762 4918 1790 Size: 406 683 37 35 811 1156 2573 Fragments arranged by size: 2573 1156 811 683 406 37 35 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5701 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2561 2959 3432 Size: 416 53 83 399 24 1144 398 473 Cuts at: 3432 4390 442 Size: 958 1753 Fragments arranged by size: 1753 1144 958 473 416 399 398 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5701 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2114 2428 2497 4361 1239 Size: 875 314 69 1864 2579 Fragments arranged by size: 2579 1864 875 314 69 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3826 4691 4989 3826 Size: 865 298 4538 Fragments arranged by size: 4538 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2050 4364 882 Size: 617 551 2314 2219 Fragments arranged by size: 2314 2219 617 551 BsaAI yAC'GTr Cuts at: 493 1204 2794 3492 493 Size: 711 1590 698 2702 Fragments arranged by size: 2702 1590 711 698 BsaBI GATnn'nnATC Cuts at: 5695 5695 Size: 5701 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2367 3508 3890 4525 Size: 53 83 186 936 834 1141 382 635 Cuts at: 4525 275 Size: 1451 Fragments arranged by size: 1451 1141 936 834 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4134 4532 4806 4953 5364 4134 Size: 398 274 147 411 4471 Fragments arranged by size: 4471 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2426 2456 1178 Size: 30 165 30 1023 30 4423 Fragments arranged by size: 4423 1023 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2990 1186 Size: 1804 3897 BseYI C'CCAG_C Cuts at: 2279 2384 2726 4904 5400 2279 Size: 105 342 2178 496 2580 Fragments arranged by size: 2580 2178 496 342 105 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2258 2489 1163 Size: 573 522 231 4375 Fragments arranged by size: 4375 573 522 231 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3151 3912 4940 1540 Size: 275 186 1150 761 1028 2301 Fragments arranged by size: 2301 1150 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2325 2561 3762 3847 4918 2325 Size: 236 1201 85 1071 3108 Fragments arranged by size: 3108 1201 1071 236 85 BsmI GAATG_Cn' Cuts at: 3212 3305 3212 Size: 93 5608 BsmBI CGTCTCn'nnnn_ Cuts at: 2358 2358 Size: 5701 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2196 2280 2325 2561 2879 2916 2951 Size: 399 406 84 45 236 318 37 35 Cuts at: 2951 3020 3762 3847 4918 1391 Size: 69 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 406 399 318 236 85 84 69 45 37 35 BspEI T'CCGG_A Cuts at: 5364 5364 Size: 5701 BspHI T'CATG_A Cuts at: 2895 3590 5320 2895 Size: 695 1730 3276 Fragments arranged by size: 3276 1730 695 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2056 2257 3056 844 Size: 1212 201 799 3489 Fragments arranged by size: 3489 1212 799 201 BsrBI CCG'CTC Cuts at: 1394 1823 3588 1394 Size: 429 1765 3507 Fragments arranged by size: 3507 1765 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4196 4370 65 Size: 1183 2948 174 1396 Fragments arranged by size: 2948 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4345 4532 1700 Size: 110 2535 187 2869 Fragments arranged by size: 2869 2535 187 110 BsrGI T'GTAC_A Cuts at: 96 2718 96 Size: 2622 3079 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5701 BssSI C'ACGA_G Cuts at: 3761 4773 3761 Size: 1012 4689 BstAPI GCAn_nnn'nTGC Cuts at: 794 2381 794 Size: 1587 4114 BstBI TT'CG_AA Cuts at: 4505 4505 Size: 5701 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5701 BstXI CCAn_nnnn'nTGG Cuts at: 2147 2147 Size: 5701 BstYI r'GATC_y Cuts at: 1972 2303 2730 3031 3398 3783 3800 5252 5338 Size: 331 427 301 367 385 17 1452 86 Cuts at: 5338 5350 5696 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 427 385 367 346 331 301 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2146 513 Size: 552 1081 4068 Fragments arranged by size: 4068 1081 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4962 Fragments arranged by size: 4962 683 56 BtsI GCAGTG_nn' Cuts at: 2660 2960 2993 3217 4011 4031 2660 Size: 300 33 224 794 20 4330 Fragments arranged by size: 4330 794 300 224 33 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5701 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5666 DraI TTT'AAA Cuts at: 3005 3005 Size: 5701 DraIII CAC_nnn'GTG Cuts at: 2797 2943 2797 Size: 146 5555 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2141 4708 809 Size: 507 825 2567 1802 Fragments arranged by size: 2567 1802 825 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2884 3148 4037 Size: 54 1425 50 275 18 1054 264 889 Cuts at: 4037 5666 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1054 889 275 264 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3148 1537 Size: 275 1336 4090 Fragments arranged by size: 4090 1336 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2566 3631 1353 Size: 1213 1065 3423 Fragments arranged by size: 3423 1213 1065 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4288 4662 4808 387 Size: 1064 138 2699 374 146 1280 Fragments arranged by size: 2699 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2323 2559 2323 Size: 236 5465 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2114 2428 2497 3764 4361 5148 5416 Size: 393 482 314 69 1267 597 787 268 Cuts at: 5416 1239 Size: 1524 Fragments arranged by size: 1524 1267 787 597 482 393 314 268 69 EcoO109I rG'GnC_Cy Cuts at: 2192 2193 2482 2779 2192 Size: 1 289 297 5114 Fragments arranged by size: 5114 297 289 1 EcoRI G'AATT_C Cuts at: 3010 3388 3010 Size: 378 5323 FspI TGC'GCA Cuts at: 4207 4207 Size: 5701 HaeII r_GCGC'y Cuts at: 1678 4848 1678 Size: 3170 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2291 2323 2579 2611 2715 Size: 32 135 32 719 32 256 32 104 Cuts at: 2715 2747 4342 4374 4416 4448 1373 Size: 32 1595 32 42 32 2626 Fragments arranged by size: 2626 1595 719 256 135 104 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3045 3291 669 Size: 988 281 1107 246 3079 Fragments arranged by size: 3079 1107 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5701 HpaI GTT'AAC Cuts at: 3291 3291 Size: 5701 KpnI G_GTAC'C Cuts at: 3026 3026 Size: 5701 MfeI C'AATT_G Cuts at: 3300 3300 Size: 5701 MluI A'CGCG_T Cuts at: 5577 5577 Size: 5701 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4815 4999 1305 Size: 3510 184 2007 Fragments arranged by size: 3510 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2886 10 Size: 54 1425 343 1054 2825 Fragments arranged by size: 2825 1425 1054 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2511 3659 4018 4177 518 Size: 648 1345 1148 359 159 2042 Fragments arranged by size: 2042 1345 1148 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2283 3117 3794 4942 5187 1606 Size: 677 834 677 1148 245 2120 Fragments arranged by size: 2120 1148 834 677 677 245 NcoI C'CATG_G Cuts at: 513 1065 2146 513 Size: 552 1081 4068 Fragments arranged by size: 4068 1081 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5701 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5701 NotI GC'GGCC_GC Cuts at: 3148 3148 Size: 5701 NspI r_CATG'y Cuts at: 1132 1229 3059 4604 1132 Size: 97 1830 1545 2229 Fragments arranged by size: 2229 1830 1545 97 PasI CC'CwG_GG Cuts at: 1348 2332 1348 Size: 984 4717 PciI A'CATG_T Cuts at: 4600 4600 Size: 5701 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5701 PfoI T'CCnGG_A Cuts at: 2130 2130 Size: 5701 PmeI GTTT'AAAC Cuts at: 3005 3005 Size: 5701 PmlI CAC'GTG Cuts at: 2794 2794 Size: 5701 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2426 2458 3770 3802 5283 5315 2426 Size: 32 1312 32 1481 32 2812 Fragments arranged by size: 2812 1481 1312 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2482 2779 2482 Size: 297 5404 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5701 PsiI TTA'TAA Cuts at: 2538 3271 2538 Size: 733 4968 PspOMI G'GGCC_C Cuts at: 2192 2912 2192 Size: 720 4981 PstI C_TGCA'G Cuts at: 830 2118 2320 2377 2823 3053 830 Size: 1288 202 57 446 230 3478 Fragments arranged by size: 3478 1288 446 230 202 57 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5701 SacI G_AGCT'C Cuts at: 2325 2561 2325 Size: 236 5465 SalI G'TCGA_C Cuts at: 1936 3043 1936 Size: 1107 4594 SanDI GG'GwC_CC Cuts at: 2482 2482 Size: 5701 SapI GCTCTTCn'nnn_ Cuts at: 2566 2566 Size: 5701 SbfI CC_TGCA'GG Cuts at: 2377 3053 2377 Size: 676 5025 ScaI AGT'ACT Cuts at: 3949 5661 3949 Size: 1712 3989 SexAI A'CCwGG_T Cuts at: 2889 2939 2889 Size: 50 5651 SfcI C'TryA_G Cuts at: 826 1045 2099 2114 2316 2373 2819 3049 4184 Size: 219 1054 15 202 57 446 230 1135 Cuts at: 4184 4865 5056 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1054 681 446 230 219 202 191 57 15 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5701 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5701 SmaI CCC'GGG Cuts at: 1872 3028 1872 Size: 1156 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3805 4706 4968 820 Size: 197 926 1862 901 262 1553 Fragments arranged by size: 1862 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3492 493 Size: 2999 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5701 SphI G_CATG'C Cuts at: 1132 3059 1132 Size: 1927 3774 SspI AAT'ATT Cuts at: 5 52 3625 5 Size: 47 3573 2081 Fragments arranged by size: 3573 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5701 StyI C'CwwG_G Cuts at: 513 1065 2146 2833 3140 513 Size: 552 1081 687 307 3074 Fragments arranged by size: 3074 1081 687 552 307 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2153 2419 2593 3728 3745 3898 4083 Size: 1255 573 266 174 1135 17 153 185 Cuts at: 4083 4528 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1135 573 445 266 185 174 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2718 2807 3947 Size: 274 80 33 51 1443 741 89 1140 Cuts at: 3947 5659 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1140 741 274 138 89 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5661 5693 5661 Size: 32 5669 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5701 XbaI T'CTAG_A Cuts at: 2176 3037 2176 Size: 861 4840 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5701 XmaI C'CCGG_G Cuts at: 1870 3026 1870 Size: 1156 4545 XmnI GAAnn'nnTTC Cuts at: 2159 2438 3830 2159 Size: 279 1392 4030 Fragments arranged by size: 4030 1392 279 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3509 276 Size: 53 83 186 936 1975 2468 Fragments arranged by size: 2468 1975 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI PsrI PvuII RsrII SacII SfiI SfoI SrfI SwaI XcmI