Restriction map for
pFN21AB8169
(Linear) (Six-base) MAPSORT of: KIBB8169.seq Check: 3027 from: 1 to: 996
KIBB8169.orf
With 182 enzymes: SgfI *
March 3, 2008 11:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 51 996
Size: 51 945
AccI GT'mk_AC
Cuts at: 0 854 996
Size: 854 142
AhdI GACnn_n'nnGTC
Cuts at: 0 588 996
Size: 588 408
AleI CACnn'nnGTG
Cuts at: 0 506 996
Size: 506 490
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 421 453 996
Size: 421 32 543
Fragments arranged by size:
543 421 32
AlwNI CAG_nnn'CTG
Cuts at: 0 117 996
Size: 117 879
ApaI G_GGCC'C
Cuts at: 0 191 911 996
Size: 191 720 85
Fragments arranged by size:
720 191 85
ApoI r'AATT_y
Cuts at: 0 25 996
Size: 25 971
AvaI C'yCGr_G
Cuts at: 0 486 996
Size: 486 510
BamHI G'GATC_C
Cuts at: 0 298 725 996
Size: 298 427 271
Fragments arranged by size:
427 298 271
BanI G'GyrC_C
Cuts at: 0 941 996
Size: 941 55
BanII G_rGCy'C
Cuts at: 0 191 275 320 556 911 996
Size: 191 84 45 236 355 85
Fragments arranged by size:
355 236 191 85 84 45
BbsI GAAGACnn'nnnn_
Cuts at: 0 292 996
Size: 292 704
BglI GCCn_nnn'nGGC
Cuts at: 0 9 279 878 996
Size: 9 270 599 118
Fragments arranged by size:
599 270 118 9
BlpI GC'TnA_GC
Cuts at: 0 638 996
Size: 638 358
Bme1580I G_kGCm'C
Cuts at: 0 191 874 911 946 996
Size: 191 683 37 35 50
Fragments arranged by size:
683 191 50 37 35
BmrI ACTGGGnnnn_n'
Cuts at: 0 556 954 996
Size: 556 398 42
Fragments arranged by size:
556 398 42
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 423 492 996
Size: 109 314 69 504
Fragments arranged by size:
504 314 109 69
BsaI GGTCTCn'nnnn_
Cuts at: 0 45 996
Size: 45 951
BsaAI yAC'GTr
Cuts at: 0 789 996
Size: 789 207
BsaHI Gr'CG_yC
Cuts at: 0 362 996
Size: 362 634
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 421 451 996
Size: 421 30 545
Fragments arranged by size:
545 421 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 985 996
Size: 985 11
BseYI C'CCAG_C
Cuts at: 0 274 379 721 996
Size: 274 105 342 275
Fragments arranged by size:
342 275 274 105
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 484 996
Size: 253 231 512
Fragments arranged by size:
512 253 231
BsiHKAI G_wGCw'C
Cuts at: 0 320 556 996
Size: 320 236 440
Fragments arranged by size:
440 320 236
BsmBI CGTCTCn'nnnn_
Cuts at: 0 353 996
Size: 353 643
Bsp1286I G_dGCh'C
Cuts at: 0 191 275 320 556 874 911 946 996
Size: 191 84 45 236 318 37 35 50
Fragments arranged by size:
318 236 191 84 50 45 37 35
BspHI T'CATG_A
Cuts at: 0 890 996
Size: 890 106
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 51 252 996
Size: 51 201 744
Fragments arranged by size:
744 201 51
BsrGI T'GTAC_A
Cuts at: 0 713 996
Size: 713 283
BstAPI GCAn_nnn'nTGC
Cuts at: 0 376 996
Size: 376 620
BstXI CCAn_nnnn'nTGG
Cuts at: 0 142 996
Size: 142 854
BstYI r'GATC_y
Cuts at: 0 298 725 996
Size: 298 427 271
Fragments arranged by size:
427 298 271
BtgI C'CryG_G
Cuts at: 0 141 996
Size: 141 855
BtsI GCAGTG_nn'
Cuts at: 0 655 955 988 996
Size: 655 300 33 8
Fragments arranged by size:
655 300 33 8
DraIII CAC_nnn'GTG
Cuts at: 0 792 938 996
Size: 792 146 58
Fragments arranged by size:
792 146 58
DrdI GACnn_nn'nnGTC
Cuts at: 0 136 996
Size: 136 860
EaeI y'GGCC_r
Cuts at: 0 879 996
Size: 879 117
EarI CTCTTCn'nnn_
Cuts at: 0 561 996
Size: 561 435
EcoICRI GAG'CTC
Cuts at: 0 318 554 996
Size: 318 236 442
Fragments arranged by size:
442 318 236
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 423 492 996
Size: 109 314 69 504
Fragments arranged by size:
504 314 109 69
EcoO109I rG'GnC_Cy
Cuts at: 0 187 188 477 774 996
Size: 187 1 289 297 222
Fragments arranged by size:
297 289 222 187 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 286 318 574 606 710 742 996
Size: 286 32 256 32 104 32 254
Fragments arranged by size:
286 256 254 104 32 32 32
MscI TGG'CCA
Cuts at: 0 881 996
Size: 881 115
MslI CAynn'nnrTG
Cuts at: 0 506 996
Size: 506 490
MspA1I CmG'CkG
Cuts at: 0 278 996
Size: 278 718
NcoI C'CATG_G
Cuts at: 0 141 996
Size: 141 855
PasI CC'CwG_GG
Cuts at: 0 327 996
Size: 327 669
PfoI T'CCnGG_A
Cuts at: 0 125 996
Size: 125 871
PmlI CAC'GTG
Cuts at: 0 789 996
Size: 789 207
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 421 453 996
Size: 421 32 543
Fragments arranged by size:
543 421 32
PpuMI rG'GwC_Cy
Cuts at: 0 477 774 996
Size: 477 297 222
Fragments arranged by size:
477 297 222
PsiI TTA'TAA
Cuts at: 0 533 996
Size: 533 463
PspOMI G'GGCC_C
Cuts at: 0 187 907 996
Size: 187 720 89
Fragments arranged by size:
720 187 89
PstI C_TGCA'G
Cuts at: 0 113 315 372 818 996
Size: 113 202 57 446 178
Fragments arranged by size:
446 202 178 113 57
SacI G_AGCT'C
Cuts at: 0 320 556 996
Size: 320 236 440
Fragments arranged by size:
440 320 236
SanDI GG'GwC_CC
Cuts at: 0 477 996
Size: 477 519
SapI GCTCTTCn'nnn_
Cuts at: 0 561 996
Size: 561 435
SbfI CC_TGCA'GG
Cuts at: 0 372 996
Size: 372 624
SexAI A'CCwGG_T
Cuts at: 0 884 934 996
Size: 884 50 62
Fragments arranged by size:
884 62 50
SfcI C'TryA_G
Cuts at: 0 94 109 311 368 814 996
Size: 94 15 202 57 446 182
Fragments arranged by size:
446 202 182 94 57 15
StyI C'CwwG_G
Cuts at: 0 141 828 996
Size: 141 687 168
Fragments arranged by size:
687 168 141
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 148 414 588 996
Size: 148 266 174 408
Fragments arranged by size:
408 266 174 148
TatI w'GTAC_w
Cuts at: 0 713 802 996
Size: 713 89 194
Fragments arranged by size:
713 194 89
XbaI T'CTAG_A
Cuts at: 0 171 996
Size: 171 825
XmnI GAAnn'nnTTC
Cuts at: 0 154 433 996
Size: 154 279 563
Fragments arranged by size:
563 279 154
Enzymes that do cut:
AarI AccI AhdI AleI AloI AlwNI ApaI ApoI
AvaI BamHI BanI BanII BbsI BglI BlpI Bme1580I
BmrI BpmI BsaI BsaAI BsaHI BsaXI BseRI BseYI
BsgI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrGI BstAPI
BstXI BstYI BtgI BtsI DraIII DrdI EaeI EarI
EcoICRI Eco57MI EcoO109I Hin4I MscI MslI MspA1I NcoI
PasI PfoI PmlI PpiI PpuMI PsiI PspOMI PstI
SacI SanDI SapI SbfI SexAI SfcI StyI TaqII
TatI XbaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AflIII AgeI
AlfI ApaLI AscI AseI AsiSI AvrII BaeI BbeI
BbvCI BcgI BciVI BclI BfrBI BglII BmgBI BmtI
BplI Bpu10I BpuEI BsaBI BsaWI BsiEI BsiWI BsmI
BspEI BsrBI BsrDI BsrFI BssHII BssSI BstBI BstEII
BstZ17I Bsu36I BtgZI ClaI CspCI DraI EagI EciI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HaeII
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PciI PflMI PmeI PshAI PspXI PsrI
PvuI PvuII RsrII SacII SalI ScaI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI TstI Tth111I XcmI XhoI XmaI
ZraI