(Circular) (Six-base) MAPSORT of: pFN21AB8162.seq Check: 535 from: 1 to: 5782 pFN21AB8162 5782 bp With 182 enzymes: SgfI * February 27, 2009 14:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3592 278 Size: 53 83 186 936 2056 2468 Fragments arranged by size: 2468 2056 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3125 1937 Size: 1188 4594 Acc65I G'GTAC_C Cuts at: 3103 3103 Size: 5782 AclI AA'CG_TT Cuts at: 1563 3909 4282 1563 Size: 2346 373 3063 Fragments arranged by size: 3063 2346 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2841 3845 5229 5497 1632 Size: 1209 1004 1384 268 1917 Fragments arranged by size: 1917 1384 1209 1004 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5585 AflIII A'CryG_T Cuts at: 1203 2245 4681 5658 1203 Size: 1042 2436 977 1327 Fragments arranged by size: 2436 1327 1042 977 AgeI A'CCGG_T Cuts at: 4613 4613 Size: 5782 AhdI GACnn_n'nnGTC Cuts at: 4511 4511 Size: 5782 AleI CACnn'nnGTG Cuts at: 1166 2777 1166 Size: 1611 4171 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2441 1184 Size: 1257 4525 AlwNI CAG_nnn'CTG Cuts at: 3525 5097 3525 Size: 1572 4210 ApaLI G'TGCA_C Cuts at: 2123 3839 4995 2123 Size: 1716 1156 2910 Fragments arranged by size: 2910 1716 1156 ApoI r'AATT_y Cuts at: 1493 3013 3091 3317 3469 5578 1493 Size: 1520 78 226 152 2109 1697 Fragments arranged by size: 2109 1697 1520 226 152 78 AseI AT'TA_AT Cuts at: 160 1032 4336 160 Size: 872 3304 1606 Fragments arranged by size: 3304 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5782 AvaI C'yCGr_G Cuts at: 1870 1943 2034 3107 1870 Size: 73 91 1073 4545 Fragments arranged by size: 4545 1073 91 73 BamHI G'GATC_C Cuts at: 3112 3479 3112 Size: 367 5415 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3103 4558 618 Size: 325 217 627 1316 1455 1842 Fragments arranged by size: 1842 1455 1316 627 325 217 BanII G_rGCy'C Cuts at: 1391 2918 3067 3101 1391 Size: 1527 149 34 4072 Fragments arranged by size: 4072 1527 149 34 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2139 928 Size: 971 240 4571 Fragments arranged by size: 4571 971 240 BbvCI CC'TCA_GC Cuts at: 2148 2148 Size: 5782 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2039 2073 3973 4007 1903 Size: 34 102 34 1900 34 3678 Fragments arranged by size: 3678 1900 102 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3674 4884 3674 Size: 1210 4572 BclI T'GATC_A Cuts at: 1548 1548 Size: 5782 BfrBI ATG'CAT Cuts at: 2428 2645 2428 Size: 217 5565 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4393 136 Size: 107 122 71 631 605 2721 1525 Fragments arranged by size: 2721 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5777 5777 Size: 5782 BlpI GC'TnA_GC Cuts at: 3199 3199 Size: 5782 Bme1580I G_kGCm'C Cuts at: 1790 2127 2514 2531 3843 4999 1790 Size: 337 387 17 1312 1156 2573 Fragments arranged by size: 2573 1312 1156 387 337 17 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5782 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2525 3513 4471 Size: 416 53 83 399 24 1108 988 958 Cuts at: 4471 442 Size: 1753 Fragments arranged by size: 1753 1108 988 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5782 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2077 2077 Size: 5782 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4442 1239 Size: 3203 2579 Bpu10I CC'TnA_GC Cuts at: 2148 2148 Size: 5782 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2745 3907 4772 5070 2745 Size: 1162 865 298 3457 Fragments arranged by size: 3457 1162 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2469 4445 882 Size: 617 970 1976 2219 Fragments arranged by size: 2219 1976 970 617 BsaAI yAC'GTr Cuts at: 493 1204 2248 3573 493 Size: 711 1044 1325 2702 Fragments arranged by size: 2702 1325 1044 711 BsaBI GATnn'nnATC Cuts at: 5776 5776 Size: 5782 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3589 3971 4606 275 Size: 53 83 186 936 2056 382 635 1451 Fragments arranged by size: 2056 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2311 4215 4613 4887 5034 5445 2311 Size: 1904 398 274 147 411 2648 Fragments arranged by size: 2648 1904 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5557 Fragments arranged by size: 5557 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2315 1186 Size: 1129 4653 BseYI C'CCAG_C Cuts at: 4985 5481 4985 Size: 496 5286 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2153 2453 1163 Size: 573 417 300 4492 Fragments arranged by size: 4492 573 417 300 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3232 3993 5021 1540 Size: 275 186 1231 761 1028 2301 Fragments arranged by size: 2301 1231 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2127 2656 3843 3928 4999 2127 Size: 529 1187 85 1071 2910 Fragments arranged by size: 2910 1187 1071 529 85 BsiWI C'GTAC_G Cuts at: 2540 2540 Size: 5782 BsmI GAATG_Cn' Cuts at: 2430 3293 3386 2430 Size: 863 93 4826 Fragments arranged by size: 4826 863 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2127 2514 2531 2656 2918 3067 3101 Size: 399 337 387 17 125 262 149 34 Cuts at: 3101 3843 3928 4999 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 387 337 262 149 125 85 34 17 BspEI T'CCGG_A Cuts at: 5445 5445 Size: 5782 BspHI T'CATG_A Cuts at: 3671 5401 3671 Size: 1730 4052 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2175 3137 844 Size: 1331 962 3489 Fragments arranged by size: 3489 1331 962 BsrBI CCG'CTC Cuts at: 1394 1823 2362 2685 3669 1394 Size: 429 539 323 984 3507 Fragments arranged by size: 3507 984 539 429 323 BsrDI GCAATG_nn' Cuts at: 65 1248 4277 4451 65 Size: 1183 3029 174 1396 Fragments arranged by size: 3029 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2448 4426 4613 1700 Size: 110 638 1978 187 2869 Fragments arranged by size: 2869 1978 638 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5782 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5782 BssSI C'ACGA_G Cuts at: 3842 4854 3842 Size: 1012 4770 BstAPI GCAn_nnn'nTGC Cuts at: 794 2372 794 Size: 1578 4204 BstBI TT'CG_AA Cuts at: 2596 4586 2596 Size: 1990 3792 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5782 BstYI r'GATC_y Cuts at: 1972 3112 3479 3864 3881 5333 5419 5431 5777 Size: 1140 367 385 17 1452 86 12 346 Cuts at: 5777 1972 Size: 1977 Fragments arranged by size: 1977 1452 1140 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2610 513 Size: 552 939 606 3685 Fragments arranged by size: 3685 939 606 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5043 Fragments arranged by size: 5043 683 56 BtsI GCAGTG_nn' Cuts at: 2382 2918 3298 4092 4112 2382 Size: 536 380 794 20 4052 Fragments arranged by size: 4052 794 536 380 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5782 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5747 DraI TTT'AAA Cuts at: 3086 3086 Size: 5782 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4789 809 Size: 507 3473 1802 Fragments arranged by size: 3473 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3229 4118 5747 Size: 54 1425 50 275 18 1399 889 1629 Cuts at: 5747 8 Size: 43 Fragments arranged by size: 1629 1425 1399 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3229 1537 Size: 275 1417 4090 Fragments arranged by size: 4090 1417 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2072 3712 1353 Size: 719 1640 3423 Fragments arranged by size: 3423 1640 719 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4369 4743 4889 387 Size: 1064 138 2780 374 146 1280 Fragments arranged by size: 2780 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2841 3845 4442 5229 5497 1239 Size: 393 1209 1004 597 787 268 1524 Fragments arranged by size: 1524 1209 1004 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2392 2392 Size: 5782 EcoRI G'AATT_C Cuts at: 3013 3091 3469 3013 Size: 78 378 5326 Fragments arranged by size: 5326 378 78 FspI TGC'GCA Cuts at: 4288 4288 Size: 5782 HaeII r_GCGC'y Cuts at: 1678 2847 4929 1678 Size: 1169 2082 2531 Fragments arranged by size: 2531 2082 1169 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2664 2696 4423 4455 4497 Size: 32 135 32 1092 32 1727 32 42 Cuts at: 4497 4529 1373 Size: 32 2626 Fragments arranged by size: 2626 1727 1092 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3126 3372 669 Size: 988 281 1188 246 3079 Fragments arranged by size: 3079 1188 988 281 246 HindIII A'AGCT_T Cuts at: 748 2180 2229 748 Size: 1432 49 4301 Fragments arranged by size: 4301 1432 49 HpaI GTT'AAC Cuts at: 3372 3372 Size: 5782 KpnI G_GTAC'C Cuts at: 3107 3107 Size: 5782 MfeI C'AATT_G Cuts at: 3381 3381 Size: 5782 MluI A'CGCG_T Cuts at: 5658 5658 Size: 5782 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2932 4896 5080 1305 Size: 1627 1964 184 2007 Fragments arranged by size: 2007 1964 1627 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3960 Fragments arranged by size: 3960 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2201 2777 3740 4099 4258 518 Size: 648 1035 576 963 359 159 2042 Fragments arranged by size: 2042 1035 963 648 576 359 159 MspA1I CmG'CkG Cuts at: 1606 2147 2705 2714 2861 3198 3875 5023 5268 Size: 541 558 9 147 337 677 1148 245 Cuts at: 5268 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 558 541 337 245 147 9 NaeI GCC'GGC Cuts at: 2450 2450 Size: 5782 NcoI C'CATG_G Cuts at: 513 1065 2004 2610 513 Size: 552 939 606 3685 Fragments arranged by size: 3685 939 606 552 NdeI CA'TA_TG Cuts at: 387 2647 387 Size: 2260 3522 NgoMIV G'CCGG_C Cuts at: 2448 2448 Size: 5782 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5782 NotI GC'GGCC_GC Cuts at: 3229 3229 Size: 5782 NsiI A_TGCA'T Cuts at: 2430 2647 2430 Size: 217 5565 NspI r_CATG'y Cuts at: 1132 1229 2432 3140 4685 1132 Size: 97 1203 708 1545 2229 Fragments arranged by size: 2229 1545 1203 708 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5782 PciI A'CATG_T Cuts at: 4681 4681 Size: 5782 PflMI CCAn_nnn'nTGG Cuts at: 1108 2840 1108 Size: 1732 4050 PmeI GTTT'AAAC Cuts at: 3086 3086 Size: 5782 PmlI CAC'GTG Cuts at: 2248 2248 Size: 5782 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3851 3883 5364 5396 3851 Size: 32 1481 32 4237 Fragments arranged by size: 4237 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5782 PsiI TTA'TAA Cuts at: 3352 3352 Size: 5782 PstI C_TGCA'G Cuts at: 830 2881 2935 3134 830 Size: 2051 54 199 3478 Fragments arranged by size: 3478 2051 199 54 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5782 PvuII CAG'CTG Cuts at: 2147 2861 2147 Size: 714 5068 SalI G'TCGA_C Cuts at: 1936 3124 1936 Size: 1188 4594 SbfI CC_TGCA'GG Cuts at: 3134 3134 Size: 5782 ScaI AGT'ACT Cuts at: 4030 5742 4030 Size: 1712 4070 SfcI C'TryA_G Cuts at: 826 1045 2043 2877 2931 3130 4265 4946 5137 Size: 219 998 834 54 199 1135 681 191 Cuts at: 5137 826 Size: 1471 Fragments arranged by size: 1471 1135 998 834 681 219 199 191 54 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5782 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5782 SmaI CCC'GGG Cuts at: 1872 2036 3109 1872 Size: 164 1073 4545 Fragments arranged by size: 4545 1073 164 SmlI C'TyrA_G Cuts at: 820 1017 1943 2760 3886 4787 5049 820 Size: 197 926 817 1126 901 262 1553 Fragments arranged by size: 1553 1126 926 901 817 262 197 SnaBI TAC'GTA Cuts at: 493 3573 493 Size: 3080 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5782 SphI G_CATG'C Cuts at: 1132 3140 1132 Size: 2008 3774 SspI AAT'ATT Cuts at: 5 52 3706 5 Size: 47 3654 2081 Fragments arranged by size: 3654 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5782 StyI C'CwwG_G Cuts at: 513 1065 2004 2610 3221 513 Size: 552 939 606 611 3074 Fragments arranged by size: 3074 939 611 606 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3075 3809 3826 3979 4164 4609 325 Size: 1255 1495 734 17 153 185 445 1498 Fragments arranged by size: 1498 1495 1255 734 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4028 5740 96 Size: 274 80 33 51 1443 2051 1712 138 Fragments arranged by size: 2051 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5742 5774 5742 Size: 32 5750 Tth111I GACn'n_nGTC Cuts at: 1316 2746 1316 Size: 1430 4352 XbaI T'CTAG_A Cuts at: 3118 3118 Size: 5782 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2245 2245 Size: 5782 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5782 XmaI C'CCGG_G Cuts at: 1870 2034 3107 1870 Size: 164 1073 4545 Fragments arranged by size: 4545 1073 164 XmnI GAAnn'nnTTC Cuts at: 2015 3911 2015 Size: 1896 3886 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3590 276 Size: 53 83 186 936 2056 2468 Fragments arranged by size: 2468 2056 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI PacI PfoI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI