(Circular) (Six-base) MAPSORT of: pFN21AB8158.seq Check: 6444 from: 1 to: 5836 pFN21AB8158 5836 bp With 182 enzymes: SgfI * February 27, 2009 14:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2932 3646 278 Size: 53 83 186 936 1396 714 2468 Fragments arranged by size: 2468 1396 936 714 186 83 53 AccI GT'mk_AC Cuts at: 1937 2208 2220 2640 2808 3179 1937 Size: 271 12 420 168 371 4594 Fragments arranged by size: 4594 420 371 271 168 12 Acc65I G'GTAC_C Cuts at: 2430 3157 2430 Size: 727 5109 AclI AA'CG_TT Cuts at: 1563 3963 4336 1563 Size: 2400 373 3063 Fragments arranged by size: 3063 2400 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2561 3899 5283 5551 1632 Size: 929 1338 1384 268 1917 Fragments arranged by size: 1917 1384 1338 929 268 AfeI AGC'GCT Cuts at: 2117 2117 Size: 5836 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5639 AflIII A'CryG_T Cuts at: 1203 4735 5712 1203 Size: 3532 977 1327 Fragments arranged by size: 3532 1327 977 AgeI A'CCGG_T Cuts at: 4667 4667 Size: 5836 AhdI GACnn_n'nnGTC Cuts at: 4565 4565 Size: 5836 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5836 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5836 AlwNI CAG_nnn'CTG Cuts at: 2523 3122 3579 5151 2523 Size: 599 457 1572 3208 Fragments arranged by size: 3208 1572 599 457 ApaI G_GGCC'C Cuts at: 2036 2036 Size: 5836 ApaLI G'TGCA_C Cuts at: 3893 5049 3893 Size: 1156 4680 ApoI r'AATT_y Cuts at: 1493 3145 3371 3523 5632 1493 Size: 1652 226 152 2109 1697 Fragments arranged by size: 2109 1697 1652 226 152 AseI AT'TA_AT Cuts at: 160 1032 4390 160 Size: 872 3358 1606 Fragments arranged by size: 3358 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5836 AvaI C'yCGr_G Cuts at: 1870 1943 3036 3161 1870 Size: 73 1093 125 4545 Fragments arranged by size: 4545 1093 125 73 BamHI G'GATC_C Cuts at: 3166 3533 3166 Size: 367 5469 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2079 2430 2849 3157 4612 Size: 325 217 627 292 351 419 308 1455 Cuts at: 4612 618 Size: 1842 Fragments arranged by size: 1842 1455 627 419 351 325 308 292 217 BanII G_rGCy'C Cuts at: 1391 2036 3093 3155 1391 Size: 645 1057 62 4072 Fragments arranged by size: 4072 1057 645 62 BbeI G_GCGC'C Cuts at: 2083 2083 Size: 5836 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2386 928 Size: 971 487 4378 Fragments arranged by size: 4378 971 487 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4027 4061 1903 Size: 34 50 34 2006 34 3678 Fragments arranged by size: 3678 2006 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3007 3728 4938 3007 Size: 721 1210 3905 Fragments arranged by size: 3905 1210 721 BclI T'GATC_A Cuts at: 1548 1548 Size: 5836 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4447 136 Size: 107 122 71 631 605 2775 1525 Fragments arranged by size: 2775 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5831 5831 Size: 5836 BlpI GC'TnA_GC Cuts at: 3253 3253 Size: 5836 Bme1580I G_kGCm'C Cuts at: 1790 2036 2854 3897 5053 1790 Size: 246 818 1043 1156 2573 Fragments arranged by size: 2573 1156 1043 818 246 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5836 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3567 4525 442 Size: 416 53 83 399 24 2150 958 1753 Fragments arranged by size: 2150 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5836 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4496 1239 Size: 3257 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3961 4826 5124 3961 Size: 865 298 4673 Fragments arranged by size: 4673 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4499 882 Size: 617 3000 2219 Fragments arranged by size: 3000 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3627 493 Size: 711 2423 2702 Fragments arranged by size: 2702 2423 711 BsaBI GATnn'nnATC Cuts at: 5830 5830 Size: 5836 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2080 2929 3643 4025 Size: 53 83 186 936 547 849 714 382 Cuts at: 4025 4660 275 Size: 635 1451 Fragments arranged by size: 1451 936 849 714 635 547 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4269 4667 4941 5088 5499 4269 Size: 398 274 147 411 4606 Fragments arranged by size: 4606 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2082 2112 1178 Size: 30 165 30 679 30 4902 Fragments arranged by size: 4902 679 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5836 BseYI C'CCAG_C Cuts at: 2029 2068 3084 5039 5535 2029 Size: 39 1016 1955 496 2330 Fragments arranged by size: 2330 1955 1016 496 39 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2845 1163 Size: 573 1109 4154 Fragments arranged by size: 4154 1109 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3286 4047 5075 1540 Size: 275 186 1285 761 1028 2301 Fragments arranged by size: 2301 1285 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3045 3897 3982 5053 3045 Size: 852 85 1071 3828 Fragments arranged by size: 3828 1071 852 85 BsmI GAATG_Cn' Cuts at: 3347 3440 3347 Size: 93 5743 BsmBI CGTCTCn'nnnn_ Cuts at: 2470 2920 2470 Size: 450 5386 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2036 2854 3045 3093 3155 3897 3982 Size: 399 246 818 191 48 62 742 85 Cuts at: 3982 5053 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 818 742 399 246 191 85 62 48 BspEI T'CCGG_A Cuts at: 5499 5499 Size: 5836 BspHI T'CATG_A Cuts at: 2622 3725 5455 2622 Size: 1103 1730 3003 Fragments arranged by size: 3003 1730 1103 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3191 844 Size: 2347 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3723 1394 Size: 429 1900 3507 Fragments arranged by size: 3507 1900 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4331 4505 65 Size: 1183 3083 174 1396 Fragments arranged by size: 3083 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4480 4667 1700 Size: 110 2670 187 2869 Fragments arranged by size: 2869 2670 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5836 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5836 BssSI C'ACGA_G Cuts at: 3896 4908 3896 Size: 1012 4824 BstAPI GCAn_nnn'nTGC Cuts at: 794 2136 2523 794 Size: 1342 387 4107 Fragments arranged by size: 4107 1342 387 BstBI TT'CG_AA Cuts at: 4640 4640 Size: 5836 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5836 BstXI CCAn_nnnn'nTGG Cuts at: 3074 3074 Size: 5836 BstYI r'GATC_y Cuts at: 1972 3166 3533 3918 3935 5387 5473 5485 5831 Size: 1194 367 385 17 1452 86 12 346 Cuts at: 5831 1972 Size: 1977 Fragments arranged by size: 1977 1452 1194 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3067 513 Size: 552 2002 3282 Fragments arranged by size: 3282 2002 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5097 Fragments arranged by size: 5097 683 56 BtsI GCAGTG_nn' Cuts at: 2179 2512 2554 3138 3352 4146 4166 2179 Size: 333 42 584 214 794 20 3849 Fragments arranged by size: 3849 794 584 333 214 42 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5836 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5801 DraI TTT'AAA Cuts at: 3140 3140 Size: 5836 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4843 809 Size: 507 3527 1802 Fragments arranged by size: 3527 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3283 4172 5801 Size: 54 1425 50 275 18 1453 889 1629 Cuts at: 5801 8 Size: 43 Fragments arranged by size: 1629 1453 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3283 1537 Size: 275 1471 4090 Fragments arranged by size: 4090 1471 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3766 1353 Size: 2413 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2042 2412 4423 4797 4943 387 Size: 1064 138 453 370 2011 374 146 1280 Fragments arranged by size: 2011 1280 1064 453 374 370 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2561 3899 4496 5283 5551 1239 Size: 393 929 1338 597 787 268 1524 Fragments arranged by size: 1524 1338 929 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2662 2662 Size: 5836 EcoRI G'AATT_C Cuts at: 3145 3523 3145 Size: 378 5458 EcoRV GAT'ATC Cuts at: 2455 2455 Size: 5836 FspI TGC'GCA Cuts at: 4342 4342 Size: 5836 HaeII r_GCGC'y Cuts at: 1678 2083 2119 2125 4983 1678 Size: 405 36 6 2858 2531 Fragments arranged by size: 2858 2531 405 36 6 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4477 4509 4551 4583 1373 Size: 32 135 32 2905 32 42 32 2626 Fragments arranged by size: 2905 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3180 3426 669 Size: 988 281 1242 246 3079 Fragments arranged by size: 3079 1242 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5836 HpaI GTT'AAC Cuts at: 3426 3426 Size: 5836 KasI G'GCGC_C Cuts at: 2079 2079 Size: 5836 KpnI G_GTAC'C Cuts at: 2434 3161 2434 Size: 727 5109 MfeI C'AATT_G Cuts at: 2572 3435 2572 Size: 863 4973 MluI A'CGCG_T Cuts at: 5712 5712 Size: 5836 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4950 5134 1305 Size: 3645 184 2007 Fragments arranged by size: 3645 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4014 Fragments arranged by size: 4014 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3072 3794 4153 4312 518 Size: 648 1906 722 359 159 2042 Fragments arranged by size: 2042 1906 722 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2017 2085 2183 2235 3252 3929 5077 5322 Size: 411 68 98 52 1017 677 1148 245 Cuts at: 5322 1606 Size: 2120 Fragments arranged by size: 2120 1148 1017 677 411 245 98 68 52 NarI GG'CG_CC Cuts at: 2080 2080 Size: 5836 NcoI C'CATG_G Cuts at: 513 1065 3067 513 Size: 552 2002 3282 Fragments arranged by size: 3282 2002 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5836 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5836 NotI GC'GGCC_GC Cuts at: 3283 3283 Size: 5836 NspI r_CATG'y Cuts at: 1132 1229 2870 3194 4739 1132 Size: 97 1641 324 1545 2229 Fragments arranged by size: 2229 1641 1545 324 97 PasI CC'CwG_GG Cuts at: 1348 2440 2845 3099 1348 Size: 1092 405 254 4085 Fragments arranged by size: 4085 1092 405 254 PciI A'CATG_T Cuts at: 4735 4735 Size: 5836 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5836 PfoI T'CCnGG_A Cuts at: 2670 2670 Size: 5836 PmeI GTTT'AAAC Cuts at: 3140 3140 Size: 5836 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3905 3937 5418 5450 3905 Size: 32 1481 32 4291 Fragments arranged by size: 4291 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2662 2662 Size: 5836 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5836 PsiI TTA'TAA Cuts at: 3406 3406 Size: 5836 PspOMI G'GGCC_C Cuts at: 2032 2032 Size: 5836 PstI C_TGCA'G Cuts at: 830 2519 2648 3188 830 Size: 1689 129 540 3478 Fragments arranged by size: 3478 1689 540 129 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5836 SalI G'TCGA_C Cuts at: 1936 3178 1936 Size: 1242 4594 SbfI CC_TGCA'GG Cuts at: 3188 3188 Size: 5836 ScaI AGT'ACT Cuts at: 4084 5796 4084 Size: 1712 4124 SfcI C'TryA_G Cuts at: 826 1045 2365 2515 2644 2723 2936 3184 4319 Size: 219 1320 150 129 79 213 248 1135 Cuts at: 4319 5000 5191 826 Size: 681 191 1471 Fragments arranged by size: 1471 1320 1135 681 248 219 213 191 150 129 79 SfoI GGC'GCC Cuts at: 2081 2081 Size: 5836 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5836 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5836 SmaI CCC'GGG Cuts at: 1872 3163 1872 Size: 1291 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3940 4841 5103 820 Size: 197 926 1997 901 262 1553 Fragments arranged by size: 1997 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3627 493 Size: 3134 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5836 SphI G_CATG'C Cuts at: 1132 2870 3194 1132 Size: 1738 324 3774 Fragments arranged by size: 3774 1738 324 SspI AAT'ATT Cuts at: 5 52 3760 5 Size: 47 3708 2081 Fragments arranged by size: 3708 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5836 StyI C'CwwG_G Cuts at: 513 1065 2943 3067 3275 513 Size: 552 1878 124 208 3074 Fragments arranged by size: 3074 1878 552 208 124 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2779 2837 2996 3091 3863 3880 4033 Size: 1255 1199 58 159 95 772 17 153 Cuts at: 4033 4218 4663 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1199 772 445 185 159 153 95 58 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2110 4082 5794 Size: 274 80 33 51 1443 133 1972 1712 Cuts at: 5794 96 Size: 138 Fragments arranged by size: 1972 1712 1443 274 138 133 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2082 2114 5796 5828 2082 Size: 32 3682 32 2090 Fragments arranged by size: 3682 2090 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5836 XbaI T'CTAG_A Cuts at: 3172 3172 Size: 5836 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2349 2349 Size: 5836 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5836 XmaI C'CCGG_G Cuts at: 1870 3161 1870 Size: 1291 4545 XmnI GAAnn'nnTTC Cuts at: 2999 3965 2999 Size: 966 4870 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2930 3644 276 Size: 53 83 186 936 1396 714 2468 Fragments arranged by size: 2468 1396 936 714 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI