(Circular) (Six-base) MAPSORT of: pFN21AB8155.seq Check: 9422 from: 1 to: 6085 pFN21AB8155 6085 bp With 182 enzymes: SgfI * February 27, 2009 14:40 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3028 3028 Size: 6085 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3378 3895 278 Size: 53 83 186 936 1842 517 2468 Fragments arranged by size: 2468 1842 936 517 186 83 53 AccI GT'mk_AC Cuts at: 1937 3428 1937 Size: 1491 4594 Acc65I G'GTAC_C Cuts at: 3406 3406 Size: 6085 AclI AA'CG_TT Cuts at: 1563 4212 4585 1563 Size: 2649 373 3063 Fragments arranged by size: 3063 2649 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2508 2933 4148 5532 5800 1632 Size: 876 425 1215 1384 268 1917 Fragments arranged by size: 1917 1384 1215 876 425 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5888 AflIII A'CryG_T Cuts at: 1203 4984 5961 1203 Size: 3781 977 1327 Fragments arranged by size: 3781 1327 977 AgeI A'CCGG_T Cuts at: 4916 4916 Size: 6085 AhdI GACnn_n'nnGTC Cuts at: 2776 4814 2776 Size: 2038 4047 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6085 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2820 1184 Size: 1636 4449 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2667 2699 2667 Size: 32 6053 AlwNI CAG_nnn'CTG Cuts at: 2150 2588 3828 5400 2150 Size: 438 1240 1572 2835 Fragments arranged by size: 2835 1572 1240 438 ApaI G_GGCC'C Cuts at: 2234 2234 Size: 6085 ApaLI G'TGCA_C Cuts at: 3302 4142 5298 3302 Size: 840 1156 4089 Fragments arranged by size: 4089 1156 840 ApoI r'AATT_y Cuts at: 1493 3394 3620 3772 5881 1493 Size: 1901 226 152 2109 1697 Fragments arranged by size: 2109 1901 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4639 160 Size: 872 3607 1606 Fragments arranged by size: 3607 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6085 AvaI C'yCGr_G Cuts at: 1870 1943 2120 2781 3410 1870 Size: 73 177 661 629 4545 Fragments arranged by size: 4545 661 629 177 73 BamHI G'GATC_C Cuts at: 3415 3782 3415 Size: 367 5718 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2069 3406 4861 618 Size: 325 217 627 282 1337 1455 1842 Fragments arranged by size: 1842 1455 1337 627 325 282 217 BanII G_rGCy'C Cuts at: 1391 2234 2636 3299 3404 1391 Size: 843 402 663 105 4072 Fragments arranged by size: 4072 843 663 402 105 BbeI G_GCGC'C Cuts at: 2073 2073 Size: 6085 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3310 928 Size: 971 1411 3703 Fragments arranged by size: 3703 1411 971 BbvCI CC'TCA_GC Cuts at: 2061 2061 Size: 6085 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4276 4310 1903 Size: 34 2339 34 3678 Fragments arranged by size: 3678 2339 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2026 3977 5187 2026 Size: 1951 1210 2924 Fragments arranged by size: 2924 1951 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6085 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2385 4696 136 Size: 107 122 71 631 605 713 2311 1525 Fragments arranged by size: 2311 1525 713 631 605 122 107 71 BglII A'GATC_T Cuts at: 3366 6080 3366 Size: 2714 3371 BlpI GC'TnA_GC Cuts at: 2164 2309 2581 2859 3293 3502 2164 Size: 145 272 278 434 209 4747 Fragments arranged by size: 4747 434 278 272 209 145 Bme1580I G_kGCm'C Cuts at: 1790 2234 3306 4146 5302 1790 Size: 444 1072 840 1156 2573 Fragments arranged by size: 2573 1156 1072 840 444 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6085 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2191 2770 3816 Size: 416 53 83 399 24 774 579 1046 Cuts at: 3816 4774 442 Size: 958 1753 Fragments arranged by size: 1753 1046 958 774 579 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6085 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2179 2179 Size: 6085 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3317 4745 1239 Size: 2078 1428 2579 Fragments arranged by size: 2579 2078 1428 Bpu10I CC'TnA_GC Cuts at: 2061 2061 Size: 6085 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2655 4210 5075 5373 2655 Size: 1555 865 298 3367 Fragments arranged by size: 3367 1555 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4748 882 Size: 617 3249 2219 Fragments arranged by size: 3249 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3199 3876 493 Size: 711 1995 677 2702 Fragments arranged by size: 2702 1995 711 677 BsaBI GATnn'nnATC Cuts at: 6079 6079 Size: 6085 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2070 3375 3892 4274 Size: 53 83 186 936 537 1305 517 382 Cuts at: 4274 4909 275 Size: 635 1451 Fragments arranged by size: 1451 1305 936 635 537 517 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2107 2128 2499 4518 4916 5190 5337 5748 2107 Size: 21 371 2019 398 274 147 411 2444 Fragments arranged by size: 2444 2019 411 398 371 274 147 21 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2270 2300 2522 2552 2708 Size: 30 165 30 867 30 222 30 156 Cuts at: 2708 2738 1178 Size: 30 4525 Fragments arranged by size: 4525 867 222 165 156 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2114 1186 Size: 928 5157 BseYI C'CCAG_C Cuts at: 2233 2248 3088 5288 5784 2233 Size: 15 840 2200 496 2534 Fragments arranged by size: 2534 2200 840 496 15 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3048 3179 1163 Size: 573 1312 131 4069 Fragments arranged by size: 4069 1312 573 131 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3144 3148 3535 4296 5324 1540 Size: 275 186 1143 4 387 761 1028 2301 Fragments arranged by size: 2301 1143 1028 761 387 275 186 4 BsiHKAI G_wGCw'C Cuts at: 2310 3299 3306 4146 4231 5302 2310 Size: 989 7 840 85 1071 3093 Fragments arranged by size: 3093 1071 989 840 85 7 BsmI GAATG_Cn' Cuts at: 3596 3689 3596 Size: 93 5992 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2234 2310 2636 3299 3306 3404 4146 Size: 399 444 76 326 663 7 98 742 Cuts at: 4146 4231 5302 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 663 444 399 326 98 85 76 7 BspEI T'CCGG_A Cuts at: 2107 2128 2499 5748 2107 Size: 21 371 3249 2444 Fragments arranged by size: 3249 2444 371 21 BspHI T'CATG_A Cuts at: 3267 3974 5704 3267 Size: 707 1730 3648 Fragments arranged by size: 3648 1730 707 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2402 3028 3304 3440 844 Size: 1558 626 276 136 3489 Fragments arranged by size: 3489 1558 626 276 136 BsrBI CCG'CTC Cuts at: 1394 1823 3972 1394 Size: 429 2149 3507 Fragments arranged by size: 3507 2149 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4580 4754 65 Size: 1183 3332 174 1396 Fragments arranged by size: 3332 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2436 3147 4729 4916 1700 Size: 110 626 711 1582 187 2869 Fragments arranged by size: 2869 1582 711 626 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6085 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6085 BssSI C'ACGA_G Cuts at: 2020 2255 2966 3380 4145 5157 2020 Size: 235 711 414 765 1012 2948 Fragments arranged by size: 2948 1012 765 711 414 235 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6085 BstBI TT'CG_AA Cuts at: 4889 4889 Size: 6085 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6085 BstYI r'GATC_y Cuts at: 1972 2194 2496 2612 3366 3415 3782 4167 4184 Size: 222 302 116 754 49 367 385 17 Cuts at: 4184 5636 5722 5734 6080 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 754 385 367 346 302 222 116 86 49 17 12 BtgI C'CryG_G Cuts at: 513 1065 2114 2353 513 Size: 552 1049 239 4245 Fragments arranged by size: 4245 1049 552 239 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3106 487 Size: 683 56 1880 3466 Fragments arranged by size: 3466 1880 683 56 BtsI GCAGTG_nn' Cuts at: 2350 2438 2480 2717 3601 4395 4415 2350 Size: 88 42 237 884 794 20 4020 Fragments arranged by size: 4020 884 794 237 88 42 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6085 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2243 2278 529 Size: 35 1679 35 4336 Fragments arranged by size: 4336 1679 35 35 DraI TTT'AAA Cuts at: 3389 3389 Size: 6085 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2138 5092 809 Size: 507 822 2954 1802 Fragments arranged by size: 2954 1802 822 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2823 3141 3532 Size: 54 1425 50 275 18 993 318 391 Cuts at: 3532 4421 6050 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 993 889 391 318 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3141 3532 1537 Size: 275 1329 391 4090 Fragments arranged by size: 4090 1329 391 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2196 4015 1353 Size: 843 1819 3423 Fragments arranged by size: 3423 1819 843 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2403 4672 5046 5192 387 Size: 1064 138 814 2269 374 146 1280 Fragments arranged by size: 2269 1280 1064 814 374 146 138 EcoICRI GAG'CTC Cuts at: 3297 3297 Size: 6085 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2508 2933 3317 4148 4745 5532 5800 Size: 393 876 425 384 831 597 787 268 Cuts at: 5800 1239 Size: 1524 Fragments arranged by size: 1524 876 831 787 597 425 393 384 268 EcoNI CCTnn'n_nnAGG Cuts at: 2912 2912 Size: 6085 EcoO109I rG'GnC_Cy Cuts at: 2230 2230 Size: 6085 EcoRI G'AATT_C Cuts at: 3394 3772 3394 Size: 378 5707 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2350 3034 2350 Size: 684 5401 FspI TGC'GCA Cuts at: 2444 3287 4591 2444 Size: 843 1304 3938 Fragments arranged by size: 3938 1304 843 HaeII r_GCGC'y Cuts at: 1678 2073 2371 2959 3209 5232 1678 Size: 395 298 588 250 2023 2531 Fragments arranged by size: 2531 2023 588 395 298 250 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2270 2302 2522 2554 2762 Size: 32 135 32 698 32 220 32 208 Cuts at: 2762 2794 4726 4758 4800 4832 1373 Size: 32 1932 32 42 32 2626 Fragments arranged by size: 2626 1932 698 220 208 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3429 3675 669 Size: 988 281 1491 246 3079 Fragments arranged by size: 3079 1491 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6085 HpaI GTT'AAC Cuts at: 3675 3675 Size: 6085 KasI G'GCGC_C Cuts at: 2069 2069 Size: 6085 KpnI G_GTAC'C Cuts at: 3410 3410 Size: 6085 MfeI C'AATT_G Cuts at: 3684 3684 Size: 6085 MluI A'CGCG_T Cuts at: 5961 5961 Size: 6085 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2757 3342 5199 5383 1305 Size: 1452 585 1857 184 2007 Fragments arranged by size: 2007 1857 1452 585 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2825 10 Size: 54 1425 343 993 3270 Fragments arranged by size: 3270 1425 993 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3266 4043 4402 4561 518 Size: 648 2100 777 359 159 2042 Fragments arranged by size: 2100 2042 777 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2313 2666 3247 3501 4178 5326 5571 1606 Size: 707 353 581 254 677 1148 245 2120 Fragments arranged by size: 2120 1148 707 677 581 353 254 245 NaeI GCC'GGC Cuts at: 2438 2438 Size: 6085 NarI GG'CG_CC Cuts at: 2070 2070 Size: 6085 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5533 NdeI CA'TA_TG Cuts at: 387 387 Size: 6085 NgoMIV G'CCGG_C Cuts at: 2436 2436 Size: 6085 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6085 NotI GC'GGCC_GC Cuts at: 3532 3532 Size: 6085 NspI r_CATG'y Cuts at: 1132 1229 3265 3443 4988 1132 Size: 97 2036 178 1545 2229 Fragments arranged by size: 2229 2036 1545 178 97 PasI CC'CwG_GG Cuts at: 1348 2761 1348 Size: 1413 4672 PciI A'CATG_T Cuts at: 4984 4984 Size: 6085 PflMI CCAn_nnn'nTGG Cuts at: 1108 2692 1108 Size: 1584 4501 PfoI T'CCnGG_A Cuts at: 2838 3333 2838 Size: 495 5590 PmeI GTTT'AAAC Cuts at: 3389 3389 Size: 6085 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4154 4186 5667 5699 4154 Size: 32 1481 32 4540 Fragments arranged by size: 4540 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6085 PsiI TTA'TAA Cuts at: 3655 3655 Size: 6085 PspOMI G'GGCC_C Cuts at: 2230 2230 Size: 6085 PstI C_TGCA'G Cuts at: 830 2174 2712 2883 3039 3437 830 Size: 1344 538 171 156 398 3478 Fragments arranged by size: 3478 1344 538 398 171 156 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6085 PvuII CAG'CTG Cuts at: 2313 3247 2313 Size: 934 5151 SacI G_AGCT'C Cuts at: 3299 3299 Size: 6085 SalI G'TCGA_C Cuts at: 1936 3427 1936 Size: 1491 4594 SbfI CC_TGCA'GG Cuts at: 3039 3437 3039 Size: 398 5687 ScaI AGT'ACT Cuts at: 4333 6045 4333 Size: 1712 4373 SfcI C'TryA_G Cuts at: 826 1045 2170 2708 2879 3004 3035 3433 4568 Size: 219 1125 538 171 125 31 398 1135 Cuts at: 4568 5249 5440 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1125 681 538 398 219 191 171 125 31 SfoI GGC'GCC Cuts at: 2071 2071 Size: 6085 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6085 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6085 SmaI CCC'GGG Cuts at: 1872 2122 3412 1872 Size: 250 1290 4545 Fragments arranged by size: 4545 1290 250 SmlI C'TyrA_G Cuts at: 820 1017 1943 2670 4189 5090 5352 820 Size: 197 926 727 1519 901 262 1553 Fragments arranged by size: 1553 1519 926 901 727 262 197 SnaBI TAC'GTA Cuts at: 493 3876 493 Size: 3383 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6085 SphI G_CATG'C Cuts at: 1132 3265 3443 1132 Size: 2133 178 3774 Fragments arranged by size: 3774 2133 178 SspI AAT'ATT Cuts at: 5 52 4009 5 Size: 47 3957 2081 Fragments arranged by size: 3957 2081 47 StuI AGG'CCT Cuts at: 1517 2285 3033 1517 Size: 768 748 4569 Fragments arranged by size: 4569 768 748 StyI C'CwwG_G Cuts at: 513 1065 3524 513 Size: 552 2459 3074 Fragments arranged by size: 3074 2459 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2101 4112 4129 4282 4467 4912 325 Size: 1255 521 2011 17 153 185 445 1498 Fragments arranged by size: 2011 1498 1255 521 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4331 6043 96 Size: 274 80 33 51 1443 2354 1712 138 Fragments arranged by size: 2354 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6045 6077 6045 Size: 32 6053 Tth111I GACn'n_nGTC Cuts at: 1316 2183 1316 Size: 867 5218 XbaI T'CTAG_A Cuts at: 3421 3421 Size: 6085 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2026 3085 2026 Size: 1059 5026 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6085 XmaI C'CCGG_G Cuts at: 1870 2120 3410 1870 Size: 250 1290 4545 Fragments arranged by size: 4545 1290 250 XmnI GAAnn'nnTTC Cuts at: 2996 4214 2996 Size: 1218 4867 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3376 3893 276 Size: 53 83 186 936 1842 517 2468 Fragments arranged by size: 2468 1842 936 517 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FseI FspAI NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI