(Circular) (Six-base) MAPSORT of: pFN21AB8150.seq Check: 3802 from: 1 to: 6142 pFN21AB8150 6142 bp With 182 enzymes: SgfI * February 27, 2009 14:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3952 278 Size: 53 83 186 936 2416 2468 Fragments arranged by size: 2468 2416 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3109 3485 1937 Size: 1172 376 4594 Fragments arranged by size: 4594 1172 376 Acc65I G'GTAC_C Cuts at: 3463 3463 Size: 6142 AclI AA'CG_TT Cuts at: 1563 4269 4642 1563 Size: 2706 373 3063 Fragments arranged by size: 3063 2706 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4205 5589 5857 1632 Size: 2573 1384 268 1917 Fragments arranged by size: 2573 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5945 AflIII A'CryG_T Cuts at: 1203 2575 5041 6018 1203 Size: 1372 2466 977 1327 Fragments arranged by size: 2466 1372 1327 977 AgeI A'CCGG_T Cuts at: 4973 4973 Size: 6142 AhdI GACnn_n'nnGTC Cuts at: 4871 4871 Size: 6142 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6142 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2230 1184 Size: 1046 5096 AlwNI CAG_nnn'CTG Cuts at: 2282 2675 3885 5457 2282 Size: 393 1210 1572 2967 Fragments arranged by size: 2967 1572 1210 393 ApaI G_GGCC'C Cuts at: 2648 2648 Size: 6142 ApaLI G'TGCA_C Cuts at: 4199 5355 4199 Size: 1156 4986 ApoI r'AATT_y Cuts at: 1493 2688 2711 3049 3451 3677 3829 5938 1493 Size: 1195 23 338 402 226 152 2109 1697 Fragments arranged by size: 2109 1697 1195 402 338 226 152 23 AseI AT'TA_AT Cuts at: 160 1032 3207 4696 160 Size: 872 2175 1489 1606 Fragments arranged by size: 2175 1606 1489 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6142 AvaI C'yCGr_G Cuts at: 1870 1943 2199 3467 1870 Size: 73 256 1268 4545 Fragments arranged by size: 4545 1268 256 73 BamHI G'GATC_C Cuts at: 2195 3472 3839 2195 Size: 1277 367 4498 Fragments arranged by size: 4498 1277 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3463 4918 618 Size: 325 217 627 1676 1455 1842 Fragments arranged by size: 1842 1676 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2648 2766 3461 1391 Size: 1257 118 695 4072 Fragments arranged by size: 4072 1257 695 118 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2163 2259 928 Size: 971 264 96 4811 Fragments arranged by size: 4811 971 264 96 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4333 4367 1903 Size: 34 2396 34 3678 Fragments arranged by size: 3678 2396 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4034 5244 4034 Size: 1210 4932 BclI T'GATC_A Cuts at: 1548 1548 Size: 6142 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4753 136 Size: 107 122 71 631 605 3081 1525 Fragments arranged by size: 3081 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6137 6137 Size: 6142 BlpI GC'TnA_GC Cuts at: 3559 3559 Size: 6142 Bme1580I G_kGCm'C Cuts at: 1790 2648 4203 5359 1790 Size: 858 1555 1156 2573 Fragments arranged by size: 2573 1555 1156 858 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6142 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3335 3873 4831 Size: 416 53 83 399 24 1918 538 958 Cuts at: 4831 442 Size: 1753 Fragments arranged by size: 1918 1753 958 538 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3266 1056 Size: 2210 3932 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2652 3257 4802 1239 Size: 1413 605 1545 2579 Fragments arranged by size: 2579 1545 1413 605 Bpu10I CC'TnA_GC Cuts at: 2081 2427 2081 Size: 346 5796 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2309 2810 4267 5132 5430 2309 Size: 501 1457 865 298 3021 Fragments arranged by size: 3021 1457 865 501 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2911 4805 882 Size: 617 1412 1894 2219 Fragments arranged by size: 2219 1894 1412 617 BsaAI yAC'GTr Cuts at: 493 1204 2462 3933 493 Size: 711 1258 1471 2702 Fragments arranged by size: 2702 1471 1258 711 BsaBI GATnn'nnATC Cuts at: 6136 6136 Size: 6142 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3949 4331 4966 275 Size: 53 83 186 936 2416 382 635 1451 Fragments arranged by size: 2416 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4575 4973 5247 5394 5805 4575 Size: 398 274 147 411 4912 Fragments arranged by size: 4912 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2547 2577 1178 Size: 30 165 30 1144 30 4743 Fragments arranged by size: 4743 1144 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2774 3254 1186 Size: 1588 480 4074 Fragments arranged by size: 4074 1588 480 BseYI C'CCAG_C Cuts at: 2057 5345 5841 2057 Size: 3288 496 2358 Fragments arranged by size: 3288 2358 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5569 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3592 4353 5381 1540 Size: 275 186 1591 761 1028 2301 Fragments arranged by size: 2301 1591 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2608 2766 4203 4288 5359 2608 Size: 158 1437 85 1071 3391 Fragments arranged by size: 3391 1437 1071 158 85 BsmI GAATG_Cn' Cuts at: 3003 3653 3746 3003 Size: 650 93 5399 Fragments arranged by size: 5399 650 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2608 2648 2766 3461 4203 4288 5359 Size: 399 818 40 118 695 742 85 1071 Cuts at: 5359 1391 Size: 2174 Fragments arranged by size: 2174 1071 818 742 695 399 118 85 40 BspEI T'CCGG_A Cuts at: 5805 5805 Size: 6142 BspHI T'CATG_A Cuts at: 4031 5761 4031 Size: 1730 4412 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3497 844 Size: 2653 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4029 1394 Size: 429 2206 3507 Fragments arranged by size: 3507 2206 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4637 4811 65 Size: 1183 3389 174 1396 Fragments arranged by size: 3389 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4786 4973 1700 Size: 110 2976 187 2869 Fragments arranged by size: 2976 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6142 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6142 BssSI C'ACGA_G Cuts at: 2601 4202 5214 2601 Size: 1601 1012 3529 Fragments arranged by size: 3529 1601 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6142 BstBI TT'CG_AA Cuts at: 3320 4946 3320 Size: 1626 4516 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6142 BstYI r'GATC_y Cuts at: 1972 2195 2279 2736 3472 3839 4224 4241 5693 Size: 223 84 457 736 367 385 17 1452 Cuts at: 5693 5779 5791 6137 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 736 457 385 367 346 223 86 84 17 12 Bsu36I CC'TnA_GG Cuts at: 2676 2676 Size: 6142 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4651 Fragments arranged by size: 4651 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5403 Fragments arranged by size: 5403 683 56 BtsI GCAGTG_nn' Cuts at: 2412 3658 4452 4472 2412 Size: 1246 794 20 4082 Fragments arranged by size: 4082 1246 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6142 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2388 2423 529 Size: 35 1824 35 4248 Fragments arranged by size: 4248 1824 35 35 DraI TTT'AAA Cuts at: 3446 3446 Size: 6142 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5149 809 Size: 507 3833 1802 Fragments arranged by size: 3833 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3589 4478 6107 Size: 54 1425 50 275 18 1759 889 1629 Cuts at: 6107 8 Size: 43 Fragments arranged by size: 1759 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3589 1537 Size: 275 1777 4090 Fragments arranged by size: 4090 1777 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2697 2961 4072 1353 Size: 1344 264 1111 3423 Fragments arranged by size: 3423 1344 1111 264 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4729 5103 5249 387 Size: 1064 138 3140 374 146 1280 Fragments arranged by size: 3140 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2764 2764 Size: 6142 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2652 3257 4205 4802 5589 5857 1239 Size: 393 1020 605 948 597 787 268 1524 Fragments arranged by size: 1524 1020 948 787 605 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 3063 3063 Size: 6142 EcoO109I rG'GnC_Cy Cuts at: 2644 3138 2644 Size: 494 5648 EcoRI G'AATT_C Cuts at: 3451 3829 3451 Size: 378 5764 FspI TGC'GCA Cuts at: 3023 4648 3023 Size: 1625 4517 FspAI rTGC'GCAy Cuts at: 3023 3023 Size: 6142 HaeII r_GCGC'y Cuts at: 1678 5289 1678 Size: 3611 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3125 3157 3193 3225 4783 Size: 32 135 32 1553 32 36 32 1558 Cuts at: 4783 4815 4857 4889 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1558 1553 135 42 36 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3486 3732 669 Size: 988 281 1548 246 3079 Fragments arranged by size: 3079 1548 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6142 HpaI GTT'AAC Cuts at: 3732 3732 Size: 6142 KpnI G_GTAC'C Cuts at: 3467 3467 Size: 6142 MfeI C'AATT_G Cuts at: 2821 3741 2821 Size: 920 5222 MluI A'CGCG_T Cuts at: 6018 6018 Size: 6142 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2919 5256 5440 1305 Size: 1614 2337 184 2007 Fragments arranged by size: 2337 2007 1614 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4320 Fragments arranged by size: 4320 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4100 4459 4618 518 Size: 648 2934 359 159 2042 Fragments arranged by size: 2934 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2129 2267 3302 3558 4235 5383 5628 1606 Size: 523 138 1035 256 677 1148 245 2120 Fragments arranged by size: 2120 1148 1035 677 523 256 245 138 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4651 Fragments arranged by size: 4651 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6142 NheI G'CTAG_C Cuts at: 1052 3262 1052 Size: 2210 3932 NotI GC'GGCC_GC Cuts at: 3589 3589 Size: 6142 NspI r_CATG'y Cuts at: 1132 1229 2092 2356 3500 5045 1132 Size: 97 863 264 1144 1545 2229 Fragments arranged by size: 2229 1545 1144 863 264 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6142 PciI A'CATG_T Cuts at: 5041 5041 Size: 6142 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6142 PmeI GTTT'AAAC Cuts at: 3446 3446 Size: 6142 PmlI CAC'GTG Cuts at: 2462 2462 Size: 6142 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4211 4243 5724 5756 4211 Size: 32 1481 32 4597 Fragments arranged by size: 4597 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6142 PsiI TTA'TAA Cuts at: 3712 3712 Size: 6142 PspOMI G'GGCC_C Cuts at: 2644 2644 Size: 6142 PstI C_TGCA'G Cuts at: 830 2407 3037 3494 830 Size: 1577 630 457 3478 Fragments arranged by size: 3478 1577 630 457 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6142 PvuII CAG'CTG Cuts at: 2129 2267 3302 2129 Size: 138 1035 4969 Fragments arranged by size: 4969 1035 138 SacI G_AGCT'C Cuts at: 2766 2766 Size: 6142 SalI G'TCGA_C Cuts at: 1936 3484 1936 Size: 1548 4594 SbfI CC_TGCA'GG Cuts at: 3494 3494 Size: 6142 ScaI AGT'ACT Cuts at: 4390 6102 4390 Size: 1712 4430 SfcI C'TryA_G Cuts at: 826 1045 2403 3033 3490 4625 5306 5497 826 Size: 219 1358 630 457 1135 681 191 1471 Fragments arranged by size: 1471 1358 1135 681 630 457 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6142 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6142 SmaI CCC'GGG Cuts at: 1872 3469 1872 Size: 1597 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2288 2789 4246 5147 5409 820 Size: 197 926 345 501 1457 901 262 1553 Fragments arranged by size: 1553 1457 926 901 501 345 262 197 SnaBI TAC'GTA Cuts at: 493 3933 493 Size: 3440 2702 SpeI A'CTAG_T Cuts at: 152 2878 3251 152 Size: 2726 373 3043 Fragments arranged by size: 3043 2726 373 SphI G_CATG'C Cuts at: 1132 2092 3500 1132 Size: 960 1408 3774 Fragments arranged by size: 3774 1408 960 SspI AAT'ATT Cuts at: 5 52 4066 5 Size: 47 4014 2081 Fragments arranged by size: 4014 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6142 StyI C'CwwG_G Cuts at: 513 1065 2004 2525 2901 3581 513 Size: 552 939 521 376 680 3074 Fragments arranged by size: 3074 939 680 552 521 376 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 1997 4169 4186 4339 4524 4969 325 Size: 1255 417 2172 17 153 185 445 1498 Fragments arranged by size: 2172 1498 1255 445 417 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2875 4388 6100 Size: 274 80 33 51 1443 898 1513 1712 Cuts at: 6100 96 Size: 138 Fragments arranged by size: 1712 1513 1443 898 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2088 2120 2187 2219 2547 2579 2801 2833 3165 Size: 32 67 32 328 32 222 32 332 Cuts at: 3165 3197 3327 3359 6102 6134 2088 Size: 32 130 32 2743 32 2096 Fragments arranged by size: 2743 2096 332 328 222 130 67 32 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6142 XbaI T'CTAG_A Cuts at: 3478 3478 Size: 6142 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6142 XmaI C'CCGG_G Cuts at: 1870 3467 1870 Size: 1597 4545 XmnI GAAnn'nnTTC Cuts at: 4271 4271 Size: 6142 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3950 276 Size: 53 83 186 936 2416 2468 Fragments arranged by size: 2468 2416 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FalI FseI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI