(Circular) (Six-base) MAPSORT of: pFN21AB8148.seq Check: 2065 from: 1 to: 6334 pFN21AB8148 6334 bp With 182 enzymes: SgfI * February 27, 2009 14:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4144 278 Size: 53 83 186 936 2608 2468 Fragments arranged by size: 2608 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3677 1937 Size: 1740 4594 Acc65I G'GTAC_C Cuts at: 3655 3655 Size: 6334 AclI AA'CG_TT Cuts at: 1563 4461 4834 1563 Size: 2898 373 3063 Fragments arranged by size: 3063 2898 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 1999 2693 3441 3501 3531 3594 4397 5781 Size: 367 694 748 60 30 63 803 1384 Cuts at: 5781 6049 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 803 748 694 367 268 63 60 30 AfeI AGC'GCT Cuts at: 2988 3040 2988 Size: 52 6282 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6137 AflIII A'CryG_T Cuts at: 1203 5233 6210 1203 Size: 4030 977 1327 Fragments arranged by size: 4030 1327 977 AgeI A'CCGG_T Cuts at: 5165 5165 Size: 6334 AhdI GACnn_n'nnGTC Cuts at: 2254 2917 5063 2254 Size: 663 2146 3525 Fragments arranged by size: 3525 2146 663 AleI CACnn'nnGTG Cuts at: 1166 3215 1166 Size: 2049 4285 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6334 AlwNI CAG_nnn'CTG Cuts at: 3010 4077 5649 3010 Size: 1067 1572 3695 Fragments arranged by size: 3695 1572 1067 ApaLI G'TGCA_C Cuts at: 2075 2327 2681 4391 5547 2075 Size: 252 354 1710 1156 2862 Fragments arranged by size: 2862 1710 1156 354 252 ApoI r'AATT_y Cuts at: 1493 3643 3869 4021 6130 1493 Size: 2150 226 152 2109 1697 Fragments arranged by size: 2150 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4888 160 Size: 872 3856 1606 Fragments arranged by size: 3856 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6334 AvaI C'yCGr_G Cuts at: 1870 1943 2070 2630 3659 1870 Size: 73 127 560 1029 4545 Fragments arranged by size: 4545 1029 560 127 73 BamHI G'GATC_C Cuts at: 3664 4031 3664 Size: 367 5967 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2137 2597 3655 5110 618 Size: 325 217 627 350 460 1058 1455 1842 Fragments arranged by size: 1842 1455 1058 627 460 350 325 217 BanII G_rGCy'C Cuts at: 1391 2631 3251 3414 3653 1391 Size: 1240 620 163 239 4072 Fragments arranged by size: 4072 1240 620 239 163 BbeI G_GCGC'C Cuts at: 2141 2601 2141 Size: 460 5874 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2139 928 Size: 971 240 5123 Fragments arranged by size: 5123 971 240 BbvCI CC'TCA_GC Cuts at: 2832 3393 2832 Size: 561 5773 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4525 4559 1903 Size: 34 2588 34 3678 Fragments arranged by size: 3678 2588 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4226 5436 4226 Size: 1210 5124 BclI T'GATC_A Cuts at: 1548 1548 Size: 6334 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2606 4945 136 Size: 107 122 71 631 605 934 2339 1525 Fragments arranged by size: 2339 1525 934 631 605 122 107 71 BglII A'GATC_T Cuts at: 2730 6329 2730 Size: 3599 2735 BlpI GC'TnA_GC Cuts at: 2116 2552 3751 2116 Size: 436 1199 4699 Fragments arranged by size: 4699 1199 436 Bme1580I G_kGCm'C Cuts at: 1790 2079 2331 2685 4395 5551 1790 Size: 289 252 354 1710 1156 2573 Fragments arranged by size: 2573 1710 1156 354 289 252 BmgBI CAC'GTC Cuts at: 1318 2749 1318 Size: 1431 4903 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2196 4065 5023 Size: 416 53 83 399 24 779 1869 958 Cuts at: 5023 442 Size: 1753 Fragments arranged by size: 1869 1753 958 779 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6334 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3433 3433 Size: 6334 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2068 2110 2164 2228 2270 2357 2468 2525 Size: 829 42 54 64 42 87 111 57 Cuts at: 2525 3011 3032 4994 1239 Size: 486 21 1962 2579 Fragments arranged by size: 2579 1962 829 486 111 87 64 57 54 42 42 21 Bpu10I CC'TnA_GC Cuts at: 2222 2832 3393 2222 Size: 610 561 5163 Fragments arranged by size: 5163 610 561 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2237 2762 2924 4459 5324 5622 2237 Size: 525 162 1535 865 298 2949 Fragments arranged by size: 2949 1535 865 525 298 162 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2504 3188 4997 882 Size: 617 1005 684 1809 2219 Fragments arranged by size: 2219 1809 1005 684 617 BsaAI yAC'GTr Cuts at: 493 1204 2059 4125 493 Size: 711 855 2066 2702 Fragments arranged by size: 2702 2066 855 711 BsaBI GATnn'nnATC Cuts at: 6328 6328 Size: 6334 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2066 2138 2565 2598 Size: 53 83 186 936 533 72 427 33 Cuts at: 2598 2892 4141 4523 5158 275 Size: 294 1249 382 635 1451 Fragments arranged by size: 1451 1249 936 635 533 427 382 294 186 83 72 53 33 BsaWI w'CCGG_w Cuts at: 4767 5165 5439 5586 5997 4767 Size: 398 274 147 411 5104 Fragments arranged by size: 5104 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2024 2054 1178 Size: 30 165 30 621 30 5458 Fragments arranged by size: 5458 621 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2167 2522 2558 2705 3119 3206 3365 3449 Size: 981 355 36 147 414 87 159 84 Cuts at: 3449 3452 3455 3485 3593 3596 1186 Size: 3 3 30 108 3 3924 Fragments arranged by size: 3924 981 414 355 159 147 108 87 84 36 30 3 3 3 BseYI C'CCAG_C Cuts at: 3602 5537 6033 3602 Size: 1935 496 3903 Fragments arranged by size: 3903 1935 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5761 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3784 4545 5573 1540 Size: 275 186 1783 761 1028 2301 Fragments arranged by size: 2301 1783 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2079 2331 2631 2685 3251 4395 4480 5551 2079 Size: 252 300 54 566 1144 85 1071 2862 Fragments arranged by size: 2862 1144 1071 566 300 252 85 54 BsmI GAATG_Cn' Cuts at: 3845 3938 3845 Size: 93 6241 BsmBI CGTCTCn'nnnn_ Cuts at: 2057 2057 Size: 6334 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2079 2331 2631 2685 3251 3414 3653 Size: 399 289 252 300 54 566 163 239 Cuts at: 3653 4395 4480 5551 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 566 399 300 289 252 239 163 85 54 BspEI T'CCGG_A Cuts at: 5997 5997 Size: 6334 BspHI T'CATG_A Cuts at: 4223 5953 4223 Size: 1730 4604 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2448 2532 3689 844 Size: 1604 84 1157 3489 Fragments arranged by size: 3489 1604 1157 84 BsrBI CCG'CTC Cuts at: 1394 1823 3315 4221 1394 Size: 429 1492 906 3507 Fragments arranged by size: 3507 1492 906 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4829 5003 65 Size: 1183 3581 174 1396 Fragments arranged by size: 3581 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2140 2574 4978 5165 1700 Size: 110 330 434 2404 187 2869 Fragments arranged by size: 2869 2404 434 330 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6334 BssHII G'CGCG_C Cuts at: 1925 2531 2588 2790 2899 1925 Size: 606 57 202 109 5360 Fragments arranged by size: 5360 606 202 109 57 BssSI C'ACGA_G Cuts at: 2330 4394 5406 2330 Size: 2064 1012 3258 Fragments arranged by size: 3258 2064 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6334 BstBI TT'CG_AA Cuts at: 5138 5138 Size: 6334 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6334 BstXI CCAn_nnnn'nTGG Cuts at: 2953 3260 2953 Size: 307 6027 BstYI r'GATC_y Cuts at: 1972 2430 2715 2730 3664 4031 4416 4433 5885 Size: 458 285 15 934 367 385 17 1452 Cuts at: 5885 5971 5983 6329 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 934 458 385 367 346 285 86 17 15 12 BtgI C'CryG_G Cuts at: 513 1065 2736 513 Size: 552 1671 4111 Fragments arranged by size: 4111 1671 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5595 Fragments arranged by size: 5595 683 56 BtsI GCAGTG_nn' Cuts at: 3850 4644 4664 3850 Size: 794 20 5520 Fragments arranged by size: 5520 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6334 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6299 DraI TTT'AAA Cuts at: 3638 3638 Size: 6334 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5341 809 Size: 507 4025 1802 Fragments arranged by size: 4025 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2808 3262 3781 Size: 54 1425 50 275 18 978 454 519 Cuts at: 3781 4670 6299 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 978 889 519 454 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3781 1537 Size: 275 1969 4090 Fragments arranged by size: 4090 1969 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2535 2682 3450 3462 3477 3498 3570 4264 Size: 1182 147 768 12 15 21 72 694 Cuts at: 4264 1353 Size: 3423 Fragments arranged by size: 3423 1182 768 694 147 72 21 15 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2468 2758 4921 5295 5441 387 Size: 1064 138 879 290 2163 374 146 1280 Fragments arranged by size: 2163 1280 1064 879 374 290 146 138 EcoICRI GAG'CTC Cuts at: 2629 3249 2629 Size: 620 5714 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 1999 2068 2110 2164 2228 2270 2357 Size: 393 367 69 42 54 64 42 87 Cuts at: 2357 2468 2525 2693 3011 3032 3441 3501 3531 Size: 111 57 168 318 21 409 60 30 Cuts at: 3531 3594 4397 4994 5781 6049 1239 Size: 63 803 597 787 268 1524 Fragments arranged by size: 1524 803 787 597 409 393 367 318 268 168 111 87 69 64 63 60 57 54 42 42 30 21 EcoNI CCTnn'n_nnAGG Cuts at: 2225 2225 Size: 6334 EcoO109I rG'GnC_Cy Cuts at: 2290 2353 2290 Size: 63 6271 EcoRI G'AATT_C Cuts at: 3643 4021 3643 Size: 378 5956 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2869 2869 Size: 6334 FspI TGC'GCA Cuts at: 2093 2519 4840 2093 Size: 426 2321 3587 Fragments arranged by size: 3587 2321 426 HaeII r_GCGC'y Cuts at: 1678 2056 2141 2417 2483 2601 2615 2877 2990 Size: 378 85 276 66 118 14 262 113 Cuts at: 2990 3042 5481 1678 Size: 52 2439 2531 Fragments arranged by size: 2531 2439 378 276 262 118 113 85 66 52 14 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2188 2220 2240 2272 2648 Size: 32 135 32 616 32 20 32 376 Cuts at: 2648 2680 2903 2917 2935 2949 4975 5007 5049 Size: 32 223 14 18 14 2026 32 42 Cuts at: 5049 5081 1373 Size: 32 2626 Fragments arranged by size: 2626 2026 616 376 223 135 42 32 32 32 32 32 32 32 20 18 14 14 HincII GTy'rAC Cuts at: 669 1657 1938 3678 3924 669 Size: 988 281 1740 246 3079 Fragments arranged by size: 3079 1740 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6334 HpaI GTT'AAC Cuts at: 3924 3924 Size: 6334 KasI G'GCGC_C Cuts at: 2137 2597 2137 Size: 460 5874 KpnI G_GTAC'C Cuts at: 3659 3659 Size: 6334 MfeI C'AATT_G Cuts at: 3933 3933 Size: 6334 MluI A'CGCG_T Cuts at: 6210 6210 Size: 6334 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5448 5632 1305 Size: 4143 184 2007 Fragments arranged by size: 4143 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4512 Fragments arranged by size: 4512 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3215 4292 4651 4810 518 Size: 648 2049 1077 359 159 2042 Fragments arranged by size: 2049 2042 1077 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2097 3001 3479 3750 4427 5575 5820 1606 Size: 491 904 478 271 677 1148 245 2120 Fragments arranged by size: 2120 1148 904 677 491 478 271 245 NaeI GCC'GGC Cuts at: 2576 2576 Size: 6334 NarI GG'CG_CC Cuts at: 2138 2598 2138 Size: 460 5874 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5782 NdeI CA'TA_TG Cuts at: 387 387 Size: 6334 NgoMIV G'CCGG_C Cuts at: 2574 2574 Size: 6334 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6334 NotI GC'GGCC_GC Cuts at: 3781 3781 Size: 6334 NspI r_CATG'y Cuts at: 1132 1229 2824 2970 3692 5237 1132 Size: 97 1595 146 722 1545 2229 Fragments arranged by size: 2229 1595 1545 722 146 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6334 PciI A'CATG_T Cuts at: 5233 5233 Size: 6334 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6334 PfoI T'CCnGG_A Cuts at: 2286 2355 2286 Size: 69 6265 PmeI GTTT'AAAC Cuts at: 3638 3638 Size: 6334 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2825 2857 4403 4435 5916 5948 2825 Size: 32 1546 32 1481 32 3211 Fragments arranged by size: 3211 1546 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2290 2353 2290 Size: 63 6271 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6334 PsiI TTA'TAA Cuts at: 3904 3904 Size: 6334 PstI C_TGCA'G Cuts at: 830 2529 2709 3006 3686 830 Size: 1699 180 297 680 3478 Fragments arranged by size: 3478 1699 680 297 180 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6334 PvuII CAG'CTG Cuts at: 3001 3479 3001 Size: 478 5856 SacI G_AGCT'C Cuts at: 2631 3251 2631 Size: 620 5714 SalI G'TCGA_C Cuts at: 1936 3676 1936 Size: 1740 4594 SbfI CC_TGCA'GG Cuts at: 2529 3686 2529 Size: 1157 5177 ScaI AGT'ACT Cuts at: 4582 6294 4582 Size: 1712 4622 SfcI C'TryA_G Cuts at: 826 1045 2101 2525 2705 3002 3682 4817 5498 Size: 219 1056 424 180 297 680 1135 681 Cuts at: 5498 5689 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1056 681 680 424 297 219 191 180 SfoI GGC'GCC Cuts at: 2139 2599 2139 Size: 460 5874 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6334 SgrAI Cr'CCGG_yG Cuts at: 1700 2140 1700 Size: 440 5894 SmaI CCC'GGG Cuts at: 1872 2072 3661 1872 Size: 200 1589 4545 Fragments arranged by size: 4545 1589 200 SmlI C'TyrA_G Cuts at: 820 1017 1943 2252 2630 2777 2939 4438 5339 Size: 197 926 309 378 147 162 1499 901 Cuts at: 5339 5601 820 Size: 262 1553 Fragments arranged by size: 1553 1499 926 901 378 309 262 197 162 147 SnaBI TAC'GTA Cuts at: 493 4125 493 Size: 3632 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6334 SphI G_CATG'C Cuts at: 1132 2970 3692 1132 Size: 1838 722 3774 Fragments arranged by size: 3774 1838 722 SspI AAT'ATT Cuts at: 5 52 4258 5 Size: 47 4206 2081 Fragments arranged by size: 4206 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6334 StyI C'CwwG_G Cuts at: 513 1065 2907 3387 3402 3498 3773 513 Size: 552 1842 480 15 96 275 3074 Fragments arranged by size: 3074 1842 552 480 275 96 15 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2882 4361 4378 4531 4716 5161 325 Size: 1255 1302 1479 17 153 185 445 1498 Fragments arranged by size: 1498 1479 1302 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4580 6292 96 Size: 274 80 33 51 1443 2603 1712 138 Fragments arranged by size: 2603 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6294 6326 6294 Size: 32 6302 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6334 XbaI T'CTAG_A Cuts at: 3670 3670 Size: 6334 XhoI C'TCGA_G Cuts at: 1943 2630 1943 Size: 687 5647 XmaI C'CCGG_G Cuts at: 1870 2070 3659 1870 Size: 200 1589 4545 Fragments arranged by size: 4545 1589 200 XmnI GAAnn'nnTTC Cuts at: 4463 4463 Size: 6334 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4142 276 Size: 53 83 186 936 2608 2468 Fragments arranged by size: 2608 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoRV FseI FspAI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI