(Circular) (Six-base) MAPSORT of: pFN21AB8140.seq Check: 1312 from: 1 to: 5518 pFN21AB8140 5518 bp With 182 enzymes: SgfI * February 27, 2009 14:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2288 3328 278 Size: 53 83 186 936 752 1040 2468 Fragments arranged by size: 2468 1040 936 752 186 83 53 AccI GT'mk_AC Cuts at: 1937 2310 2861 1937 Size: 373 551 4594 Fragments arranged by size: 4594 551 373 Acc65I G'GTAC_C Cuts at: 2839 2839 Size: 5518 AclI AA'CG_TT Cuts at: 1563 2237 3645 4018 1563 Size: 674 1408 373 3063 Fragments arranged by size: 3063 1408 674 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2366 2391 2531 3581 4965 5233 1632 Size: 734 25 140 1050 1384 268 1917 Fragments arranged by size: 1917 1384 1050 734 268 140 25 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5321 AflIII A'CryG_T Cuts at: 1203 4417 5394 1203 Size: 3214 977 1327 Fragments arranged by size: 3214 1327 977 AgeI A'CCGG_T Cuts at: 4349 4349 Size: 5518 AhdI GACnn_n'nnGTC Cuts at: 4247 4247 Size: 5518 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5518 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2571 1184 Size: 1387 4131 AlwNI CAG_nnn'CTG Cuts at: 2092 2593 3261 4833 2092 Size: 501 668 1572 2777 Fragments arranged by size: 2777 1572 668 501 ApaLI G'TGCA_C Cuts at: 3575 4731 3575 Size: 1156 4362 ApoI r'AATT_y Cuts at: 1493 2702 2827 3053 3205 5314 1493 Size: 1209 125 226 152 2109 1697 Fragments arranged by size: 2109 1697 1209 226 152 125 AseI AT'TA_AT Cuts at: 160 1032 4072 160 Size: 872 3040 1606 Fragments arranged by size: 3040 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5518 AvaI C'yCGr_G Cuts at: 1870 1943 2843 1870 Size: 73 900 4545 Fragments arranged by size: 4545 900 73 BamHI G'GATC_C Cuts at: 2848 3215 2848 Size: 367 5151 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2045 2054 2839 4294 618 Size: 325 217 627 258 9 785 1455 1842 Fragments arranged by size: 1842 1455 785 627 325 258 217 9 BanII G_rGCy'C Cuts at: 1391 2774 2837 1391 Size: 1383 63 4072 Fragments arranged by size: 4072 1383 63 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2152 2397 928 Size: 971 253 245 4049 Fragments arranged by size: 4049 971 253 245 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3709 3743 1903 Size: 34 1772 34 3678 Fragments arranged by size: 3678 1772 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3410 4620 3410 Size: 1210 4308 BclI T'GATC_A Cuts at: 1548 1548 Size: 5518 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4129 136 Size: 107 122 71 631 605 2457 1525 Fragments arranged by size: 2457 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5513 5513 Size: 5518 BlpI GC'TnA_GC Cuts at: 2673 2935 2673 Size: 262 5256 Bme1580I G_kGCm'C Cuts at: 1790 2057 3579 4735 1790 Size: 267 1522 1156 2573 Fragments arranged by size: 2573 1522 1156 267 BmgBI CAC'GTC Cuts at: 1318 2767 1318 Size: 1449 4069 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3249 4207 442 Size: 416 53 83 399 24 1832 958 1753 Fragments arranged by size: 1832 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5518 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2615 2756 4178 1239 Size: 1376 141 1422 2579 Fragments arranged by size: 2579 1422 1376 141 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3643 4508 4806 3643 Size: 865 298 4355 Fragments arranged by size: 4355 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4181 882 Size: 617 2682 2219 Fragments arranged by size: 2682 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3309 493 Size: 711 2105 2702 Fragments arranged by size: 2702 2105 711 BsaBI GATnn'nnATC Cuts at: 5512 5512 Size: 5518 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2285 3325 3707 4342 Size: 53 83 186 936 752 1040 382 635 Cuts at: 4342 275 Size: 1451 Fragments arranged by size: 1451 1040 936 752 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2175 3951 4349 4623 4770 5181 2175 Size: 1776 398 274 147 411 2512 Fragments arranged by size: 2512 1776 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5293 Fragments arranged by size: 5293 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5518 BseYI C'CCAG_C Cuts at: 2639 2677 4721 5217 2639 Size: 38 2044 496 2940 Fragments arranged by size: 2940 2044 496 38 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2460 2761 1163 Size: 573 724 301 3920 Fragments arranged by size: 3920 724 573 301 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2968 3729 4757 1540 Size: 275 186 967 761 1028 2301 Fragments arranged by size: 2301 1028 967 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3579 3664 4735 3579 Size: 85 1071 4362 Fragments arranged by size: 4362 1071 85 BsmI GAATG_Cn' Cuts at: 2444 3029 3122 2444 Size: 585 93 4840 Fragments arranged by size: 4840 585 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2057 2774 2837 3579 3664 4735 1391 Size: 399 267 717 63 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 717 399 267 85 63 BspEI T'CCGG_A Cuts at: 2175 5181 2175 Size: 3006 2512 BspHI T'CATG_A Cuts at: 3407 5137 3407 Size: 1730 3788 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2873 844 Size: 2029 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2395 3405 1394 Size: 429 572 1010 3507 Fragments arranged by size: 3507 1010 572 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2565 4013 4187 65 Size: 1183 1317 1448 174 1396 Fragments arranged by size: 1448 1396 1317 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4162 4349 1700 Size: 110 2352 187 2869 Fragments arranged by size: 2869 2352 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5518 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5518 BssSI C'ACGA_G Cuts at: 3578 4590 3578 Size: 1012 4506 BstAPI GCAn_nnn'nTGC Cuts at: 794 2092 794 Size: 1298 4220 BstBI TT'CG_AA Cuts at: 4322 4322 Size: 5518 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5518 BstXI CCAn_nnnn'nTGG Cuts at: 2647 2647 Size: 5518 BstYI r'GATC_y Cuts at: 1972 2848 3215 3600 3617 5069 5155 5167 5513 Size: 876 367 385 17 1452 86 12 346 Cuts at: 5513 1972 Size: 1977 Fragments arranged by size: 1977 1452 876 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2311 2311 Size: 5518 BtgI C'CryG_G Cuts at: 513 1065 2004 2013 513 Size: 552 939 9 4018 Fragments arranged by size: 4018 939 552 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4779 Fragments arranged by size: 4779 683 56 BtsI GCAGTG_nn' Cuts at: 2661 2749 3034 3828 3848 2661 Size: 88 285 794 20 4331 Fragments arranged by size: 4331 794 285 88 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5518 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5483 DraI TTT'AAA Cuts at: 2822 2822 Size: 5518 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4525 809 Size: 507 3209 1802 Fragments arranged by size: 3209 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2362 2965 3854 Size: 54 1425 50 275 18 532 603 889 Cuts at: 3854 5483 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 603 532 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2965 1537 Size: 275 1153 4090 Fragments arranged by size: 4090 1153 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2752 3448 1353 Size: 1399 696 3423 Fragments arranged by size: 3423 1399 696 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4105 4479 4625 387 Size: 1064 138 2516 374 146 1280 Fragments arranged by size: 2516 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2366 2391 2531 2615 2756 3581 4178 Size: 393 734 25 140 84 141 825 597 Cuts at: 4178 4965 5233 1239 Size: 787 268 1524 Fragments arranged by size: 1524 825 787 734 597 393 268 141 140 84 25 EcoRI G'AATT_C Cuts at: 2702 2827 3205 2702 Size: 125 378 5015 Fragments arranged by size: 5015 378 125 FspI TGC'GCA Cuts at: 4024 4024 Size: 5518 HaeII r_GCGC'y Cuts at: 1678 4665 1678 Size: 2987 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2756 2788 4159 4191 4233 Size: 32 135 32 1184 32 1371 32 42 Cuts at: 4233 4265 1373 Size: 32 2626 Fragments arranged by size: 2626 1371 1184 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2259 2320 2862 3108 669 Size: 988 281 321 61 542 246 3079 Fragments arranged by size: 3079 988 542 321 281 246 61 HindIII A'AGCT_T Cuts at: 748 748 Size: 5518 HpaI GTT'AAC Cuts at: 2259 3108 2259 Size: 849 4669 KpnI G_GTAC'C Cuts at: 2843 2843 Size: 5518 MfeI C'AATT_G Cuts at: 3117 3117 Size: 5518 MluI A'CGCG_T Cuts at: 5394 5394 Size: 5518 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4632 4816 1305 Size: 3327 184 2007 Fragments arranged by size: 3327 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3696 Fragments arranged by size: 3696 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3476 3835 3994 518 Size: 648 2310 359 159 2042 Fragments arranged by size: 2310 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2015 2301 2593 2677 2934 3611 4759 5004 Size: 409 286 292 84 257 677 1148 245 Cuts at: 5004 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 409 292 286 257 245 84 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4027 Fragments arranged by size: 4027 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5518 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5518 NotI GC'GGCC_GC Cuts at: 2965 2965 Size: 5518 NspI r_CATG'y Cuts at: 1132 1229 2876 4421 1132 Size: 97 1647 1545 2229 Fragments arranged by size: 2229 1647 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5518 PciI A'CATG_T Cuts at: 4417 4417 Size: 5518 PflMI CCAn_nnn'nTGG Cuts at: 1108 2791 1108 Size: 1683 3835 PfoI T'CCnGG_A Cuts at: 2228 2733 2784 2228 Size: 505 51 4962 Fragments arranged by size: 4962 505 51 PmeI GTTT'AAAC Cuts at: 2822 2822 Size: 5518 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3587 3619 5100 5132 3587 Size: 32 1481 32 3973 Fragments arranged by size: 3973 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2156 1613 Size: 543 4975 PsiI TTA'TAA Cuts at: 3088 3088 Size: 5518 PstI C_TGCA'G Cuts at: 830 2097 2870 830 Size: 1267 773 3478 Fragments arranged by size: 3478 1267 773 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5518 PvuII CAG'CTG Cuts at: 2301 2593 2677 2301 Size: 292 84 5142 Fragments arranged by size: 5142 292 84 SacII CC_GC'GG Cuts at: 2016 2016 Size: 5518 SalI G'TCGA_C Cuts at: 1936 2860 1936 Size: 924 4594 SbfI CC_TGCA'GG Cuts at: 2870 2870 Size: 5518 ScaI AGT'ACT Cuts at: 3766 5478 3766 Size: 1712 3806 SfcI C'TryA_G Cuts at: 826 1045 2093 2866 4001 4682 4873 826 Size: 219 1048 773 1135 681 191 1471 Fragments arranged by size: 1471 1135 1048 773 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5518 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5518 SmaI CCC'GGG Cuts at: 1872 2845 1872 Size: 973 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3622 4523 4785 820 Size: 197 926 1679 901 262 1553 Fragments arranged by size: 1679 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3309 493 Size: 2816 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5518 SphI G_CATG'C Cuts at: 1132 2876 1132 Size: 1744 3774 SspI AAT'ATT Cuts at: 5 52 3442 5 Size: 47 3390 2081 Fragments arranged by size: 3390 2081 47 StuI AGG'CCT Cuts at: 1517 2265 1517 Size: 748 4770 StyI C'CwwG_G Cuts at: 513 1065 2004 2748 2957 513 Size: 552 939 744 209 3074 Fragments arranged by size: 3074 939 744 552 209 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3545 3562 3715 3900 4345 325 Size: 1255 1965 17 153 185 445 1498 Fragments arranged by size: 1965 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3764 5476 96 Size: 274 80 33 51 1443 1787 1712 138 Fragments arranged by size: 1787 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5478 5510 5478 Size: 32 5486 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5518 XbaI T'CTAG_A Cuts at: 2854 2854 Size: 5518 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5518 XmaI C'CCGG_G Cuts at: 1870 2843 1870 Size: 973 4545 XmnI GAAnn'nnTTC Cuts at: 3647 3647 Size: 5518 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2286 3326 276 Size: 53 83 186 936 752 1040 2468 Fragments arranged by size: 2468 1040 936 752 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI