Restriction map for
pFN21AB8140
(Linear) (Six-base) MAPSORT of: KIBB8140.seq Check: 1025 from: 1 to: 813
KIBB8140.orf
With 182 enzymes: SgfI *
March 3, 2008 11:03 ..
AatII G_ACGT'C
Cuts at: 0 283 813
Size: 283 530
AccI GT'mk_AC
Cuts at: 0 305 813
Size: 305 508
AclI AA'CG_TT
Cuts at: 0 232 813
Size: 232 581
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 361 386 526 813
Size: 361 25 140 287
Fragments arranged by size:
361 287 140 25
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 566 813
Size: 566 247
AlwNI CAG_nnn'CTG
Cuts at: 0 87 588 813
Size: 87 501 225
Fragments arranged by size:
501 225 87
ApoI r'AATT_y
Cuts at: 0 697 813
Size: 697 116
BanI G'GyrC_C
Cuts at: 0 40 49 813
Size: 40 9 764
Fragments arranged by size:
764 40 9
BanII G_rGCy'C
Cuts at: 0 769 813
Size: 769 44
BbsI GAAGACnn'nnnn_
Cuts at: 0 147 392 813
Size: 147 245 421
Fragments arranged by size:
421 245 147
BlpI GC'TnA_GC
Cuts at: 0 668 813
Size: 668 145
Bme1580I G_kGCm'C
Cuts at: 0 52 813
Size: 52 761
BmgBI CAC'GTC
Cuts at: 0 762 813
Size: 762 51
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 610 751 813
Size: 610 141 62
Fragments arranged by size:
610 141 62
BsaHI Gr'CG_yC
Cuts at: 0 280 813
Size: 280 533
BsaWI w'CCGG_w
Cuts at: 0 170 813
Size: 170 643
BseYI C'CCAG_C
Cuts at: 0 634 672 813
Size: 634 38 141
Fragments arranged by size:
634 141 38
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 455 756 813
Size: 455 301 57
Fragments arranged by size:
455 301 57
BsmI GAATG_Cn'
Cuts at: 0 439 813
Size: 439 374
Bsp1286I G_dGCh'C
Cuts at: 0 52 769 813
Size: 52 717 44
Fragments arranged by size:
717 52 44
BspEI T'CCGG_A
Cuts at: 0 170 813
Size: 170 643
BsrBI CCG'CTC
Cuts at: 0 390 813
Size: 390 423
BsrDI GCAATG_nn'
Cuts at: 0 560 813
Size: 560 253
BstAPI GCAn_nnn'nTGC
Cuts at: 0 87 813
Size: 87 726
BstXI CCAn_nnnn'nTGG
Cuts at: 0 642 813
Size: 642 171
BstZ17I GTA'TAC
Cuts at: 0 306 813
Size: 306 507
BtgI C'CryG_G
Cuts at: 0 8 813
Size: 8 805
BtsI GCAGTG_nn'
Cuts at: 0 656 744 813
Size: 656 88 69
Fragments arranged by size:
656 88 69
EaeI y'GGCC_r
Cuts at: 0 357 813
Size: 357 456
EarI CTCTTCn'nnn_
Cuts at: 0 747 813
Size: 747 66
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 361 386 526 610 751 813
Size: 361 25 140 84 141 62
Fragments arranged by size:
361 141 140 84 62 25
EcoRI G'AATT_C
Cuts at: 0 697 813
Size: 697 116
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 751 783 813
Size: 751 32 30
Fragments arranged by size:
751 32 30
HincII GTy'rAC
Cuts at: 0 254 315 813
Size: 254 61 498
Fragments arranged by size:
498 254 61
HpaI GTT'AAC
Cuts at: 0 254 813
Size: 254 559
MspA1I CmG'CkG
Cuts at: 0 10 296 588 672 813
Size: 10 286 292 84 141
Fragments arranged by size:
292 286 141 84 10
PflMI CCAn_nnn'nTGG
Cuts at: 0 786 813
Size: 786 27
PfoI T'CCnGG_A
Cuts at: 0 223 728 779 813
Size: 223 505 51 34
Fragments arranged by size:
505 223 51 34
PshAI GACnn'nnGTC
Cuts at: 0 151 813
Size: 151 662
PstI C_TGCA'G
Cuts at: 0 92 813
Size: 92 721
PvuII CAG'CTG
Cuts at: 0 296 588 672 813
Size: 296 292 84 141
Fragments arranged by size:
296 292 141 84
SacII CC_GC'GG
Cuts at: 0 11 813
Size: 11 802
SfcI C'TryA_G
Cuts at: 0 88 813
Size: 88 725
StuI AGG'CCT
Cuts at: 0 260 813
Size: 260 553
StyI C'CwwG_G
Cuts at: 0 743 813
Size: 743 70
ZraI GAC'GTC
Cuts at: 0 281 813
Size: 281 532
Enzymes that do cut:
AatII AccI AclI AcuI AlfI AlwNI ApoI BanI
BanII BbsI BlpI Bme1580I BmgBI BpmI BsaHI BsaWI
BseYI BsgI BsmI Bsp1286I BspEI BsrBI BsrDI BstAPI
BstXI BstZ17I BtgI BtsI EaeI EarI Eco57MI EcoRI
Hin4I HincII HpaI MspA1I PflMI PfoI PshAI PstI
PvuII SacII SfcI StuI StyI ZraI
Enzymes that do not cut:
AarI Acc65I AfeI AflII AflIII AgeI AhdI AleI
AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbeI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BmrI BmtI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaXI BseRI BsiEI BsiHKAI BsiWI BsmBI
BspHI BspMI BsrFI BsrGI BssHII BssSI BstBI BstEII
BstYI Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI
EagI EciI EcoICRI EcoNI EcoO109I EcoRV FalI FseI
FspI FspAI HaeII HindIII KasI KpnI MfeI MluI
MmeI MscI MslI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PmeI PmlI PpiI PpuMI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI SwaI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI