(Circular) (Six-base) MAPSORT of: pFN21AB8138.seq Check: 6939 from: 1 to: 6484 pFN21AB8138 6484 bp With 182 enzymes: SgfI * February 27, 2009 14:40 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2726 2742 3473 2726 Size: 16 731 5737 Fragments arranged by size: 5737 731 16 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4294 278 Size: 53 83 186 936 2758 2468 Fragments arranged by size: 2758 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2899 3827 1937 Size: 962 928 4594 Fragments arranged by size: 4594 962 928 Acc65I G'GTAC_C Cuts at: 3002 3710 3805 3002 Size: 708 95 5681 Fragments arranged by size: 5681 708 95 AclI AA'CG_TT Cuts at: 1563 4611 4984 1563 Size: 3048 373 3063 Fragments arranged by size: 3063 3048 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4547 5931 6199 1632 Size: 2915 1384 268 1917 Fragments arranged by size: 2915 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6287 AflIII A'CryG_T Cuts at: 1203 2984 3228 3230 5383 6360 1203 Size: 1781 244 2 2153 977 1327 Fragments arranged by size: 2153 1781 1327 977 244 2 AgeI A'CCGG_T Cuts at: 5315 5315 Size: 6484 AhdI GACnn_n'nnGTC Cuts at: 2638 5213 2638 Size: 2575 3909 AleI CACnn'nnGTG Cuts at: 1166 2319 3546 1166 Size: 1153 1227 4104 Fragments arranged by size: 4104 1227 1153 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6484 AlwNI CAG_nnn'CTG Cuts at: 2127 2253 3117 3185 3781 4227 5799 2127 Size: 126 864 68 596 446 1572 2812 Fragments arranged by size: 2812 1572 864 596 446 126 68 ApaI G_GGCC'C Cuts at: 3021 3021 Size: 6484 ApaLI G'TGCA_C Cuts at: 2489 4541 5697 2489 Size: 2052 1156 3276 Fragments arranged by size: 3276 2052 1156 ApoI r'AATT_y Cuts at: 1493 3793 4019 4171 6280 1493 Size: 2300 226 152 2109 1697 Fragments arranged by size: 2300 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5038 160 Size: 872 4006 1606 Fragments arranged by size: 4006 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6484 AvaI C'yCGr_G Cuts at: 1870 1943 3376 3809 1870 Size: 73 1433 433 4545 Fragments arranged by size: 4545 1433 433 73 BamHI G'GATC_C Cuts at: 3814 4181 3814 Size: 367 6117 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2061 2369 2507 2552 2646 Size: 325 217 627 274 308 138 45 94 Cuts at: 2646 3002 3028 3669 3710 3805 5260 618 Size: 356 26 641 41 95 1455 1842 Fragments arranged by size: 1842 1455 641 627 356 325 308 274 217 138 95 94 45 41 26 BanII G_rGCy'C Cuts at: 1391 3021 3803 1391 Size: 1630 782 4072 Fragments arranged by size: 4072 1630 782 BbeI G_GCGC'C Cuts at: 3673 3673 Size: 6484 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2487 928 Size: 971 588 4925 Fragments arranged by size: 4925 971 588 BbvCI CC'TCA_GC Cuts at: 3059 3240 3059 Size: 181 6303 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4675 4709 1903 Size: 34 2738 34 3678 Fragments arranged by size: 3678 2738 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4376 5586 4376 Size: 1210 5274 BclI T'GATC_A Cuts at: 1548 1548 Size: 6484 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2307 2880 3222 Size: 107 122 71 631 605 635 573 342 Cuts at: 3222 3302 3456 5095 136 Size: 80 154 1639 1525 Fragments arranged by size: 1639 1525 635 631 605 573 342 154 122 107 80 71 BglII A'GATC_T Cuts at: 6479 6479 Size: 6484 BlpI GC'TnA_GC Cuts at: 3901 3901 Size: 6484 Bme1580I G_kGCm'C Cuts at: 1790 2066 2384 2493 2555 2651 3021 3033 4545 Size: 276 318 109 62 96 370 12 1512 Cuts at: 4545 5701 1790 Size: 1156 2573 Fragments arranged by size: 2573 1512 1156 370 318 276 109 96 62 12 BmgBI CAC'GTC Cuts at: 1318 3150 3559 1318 Size: 1832 409 4243 Fragments arranged by size: 4243 1832 409 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2673 4215 5173 Size: 416 53 83 399 24 1256 1542 958 Cuts at: 5173 442 Size: 1753 Fragments arranged by size: 1753 1542 1256 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6484 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3302 5144 1239 Size: 2063 1842 2579 Fragments arranged by size: 2579 2063 1842 Bpu10I CC'TnA_GC Cuts at: 3059 3240 3059 Size: 181 6303 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3790 4609 5474 5772 3790 Size: 819 865 298 4502 Fragments arranged by size: 4502 865 819 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3508 5147 882 Size: 617 2009 1639 2219 Fragments arranged by size: 2219 2009 1639 617 BsaAI yAC'GTr Cuts at: 493 1204 2418 2985 3231 3544 4275 493 Size: 711 1214 567 246 313 731 2702 Fragments arranged by size: 2702 1214 731 711 567 313 246 BsaBI GATnn'nnATC Cuts at: 2289 6478 2289 Size: 4189 2295 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3651 3670 3747 4291 Size: 53 83 186 936 2118 19 77 544 Cuts at: 4291 4673 5308 275 Size: 382 635 1451 Fragments arranged by size: 2118 1451 936 635 544 382 186 83 77 53 19 BsaWI w'CCGG_w Cuts at: 2629 4917 5315 5589 5736 6147 2629 Size: 2288 398 274 147 411 2966 Fragments arranged by size: 2966 2288 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6259 Fragments arranged by size: 6259 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2124 2804 3115 1186 Size: 938 680 311 4555 Fragments arranged by size: 4555 938 680 311 BseYI C'CCAG_C Cuts at: 2022 3049 3393 3510 3624 3741 3763 5687 6183 Size: 1027 344 117 114 117 22 1924 496 Cuts at: 6183 2022 Size: 2323 Fragments arranged by size: 2323 1924 1027 496 344 117 117 114 22 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2343 2720 1163 Size: 573 607 377 4927 Fragments arranged by size: 4927 607 573 377 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3934 4695 5723 1540 Size: 275 186 1933 761 1028 2301 Fragments arranged by size: 2301 1933 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2493 4545 4630 5701 2493 Size: 2052 85 1071 3276 Fragments arranged by size: 3276 2052 1071 85 BsmI GAATG_Cn' Cuts at: 2405 3995 4088 2405 Size: 1590 93 4801 Fragments arranged by size: 4801 1590 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2239 2239 Size: 6484 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2066 2384 2493 2555 2651 3021 3033 Size: 399 276 318 109 62 96 370 12 Cuts at: 3033 3803 4545 4630 5701 1391 Size: 770 742 85 1071 2174 Fragments arranged by size: 2174 1071 770 742 399 370 318 276 109 96 85 62 12 BspEI T'CCGG_A Cuts at: 2629 6147 2629 Size: 3518 2966 BspHI T'CATG_A Cuts at: 4373 6103 4373 Size: 1730 4754 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2672 2726 2742 2897 3143 3377 3473 3608 Size: 1828 54 16 155 246 234 96 135 Cuts at: 3608 3839 844 Size: 231 3489 Fragments arranged by size: 3489 1828 246 234 231 155 135 96 54 16 BsrBI CCG'CTC Cuts at: 1394 1823 4371 1394 Size: 429 2548 3507 Fragments arranged by size: 3507 2548 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4979 5153 65 Size: 1183 3731 174 1396 Fragments arranged by size: 3731 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2210 5128 5315 1700 Size: 110 400 2918 187 2869 Fragments arranged by size: 2918 2869 400 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6484 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6484 BssSI C'ACGA_G Cuts at: 4544 5556 4544 Size: 1012 5472 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6484 BstBI TT'CG_AA Cuts at: 2397 5288 2397 Size: 2891 3593 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6484 BstXI CCAn_nnnn'nTGG Cuts at: 3526 3526 Size: 6484 BstYI r'GATC_y Cuts at: 1972 3814 4181 4566 4583 6035 6121 6133 6479 Size: 1842 367 385 17 1452 86 12 346 Cuts at: 6479 1972 Size: 1977 Fragments arranged by size: 1977 1842 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2271 2303 2364 2547 2805 2973 513 Size: 552 1206 32 61 183 258 168 4024 Fragments arranged by size: 4024 1206 552 258 183 168 61 32 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5745 Fragments arranged by size: 5745 683 56 BtsI GCAGTG_nn' Cuts at: 2200 2293 2314 2326 2491 2607 2737 2962 3208 Size: 93 21 12 165 116 130 225 246 Cuts at: 3208 3254 3442 3673 4000 4794 4814 2200 Size: 46 188 231 327 794 20 3870 Fragments arranged by size: 3870 794 327 246 231 225 188 165 130 116 93 46 21 20 12 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6484 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3063 3098 529 Size: 35 2499 35 3915 Fragments arranged by size: 3915 2499 35 35 DraI TTT'AAA Cuts at: 3788 3788 Size: 6484 DraIII CAC_nnn'GTG Cuts at: 2760 2839 3028 2760 Size: 79 189 6216 Fragments arranged by size: 6216 189 79 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5491 809 Size: 507 4175 1802 Fragments arranged by size: 4175 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2212 2287 3931 Size: 54 1425 50 275 18 382 75 1644 Cuts at: 3931 4820 6449 8 Size: 889 1629 43 Fragments arranged by size: 1644 1629 1425 889 382 275 75 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3931 1537 Size: 275 2119 4090 Fragments arranged by size: 4090 2119 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3080 4414 1353 Size: 1727 1334 3423 Fragments arranged by size: 3423 1727 1334 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5071 5445 5591 387 Size: 1064 138 3482 374 146 1280 Fragments arranged by size: 3482 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3302 4547 5144 5931 6199 1239 Size: 393 1670 1245 597 787 268 1524 Fragments arranged by size: 1670 1524 1245 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2789 2789 Size: 6484 EcoO109I rG'GnC_Cy Cuts at: 2910 3017 3018 3046 3733 3760 2910 Size: 107 1 28 687 27 5634 Fragments arranged by size: 5634 687 107 28 27 1 EcoRI G'AATT_C Cuts at: 3793 4171 3793 Size: 378 6106 FspI TGC'GCA Cuts at: 2832 3700 3725 4990 2832 Size: 868 25 1265 4326 Fragments arranged by size: 4326 1265 868 25 HaeII r_GCGC'y Cuts at: 1678 3673 5631 1678 Size: 1995 1958 2531 Fragments arranged by size: 2531 1995 1958 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2155 2187 2318 2350 2624 Size: 32 135 32 583 32 131 32 274 Cuts at: 2624 2656 5125 5157 5199 5231 1373 Size: 32 2469 32 42 32 2626 Fragments arranged by size: 2626 2469 583 274 135 131 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3828 4074 669 Size: 988 281 1890 246 3079 Fragments arranged by size: 3079 1890 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6484 HpaI GTT'AAC Cuts at: 4074 4074 Size: 6484 KasI G'GCGC_C Cuts at: 3669 3669 Size: 6484 KpnI G_GTAC'C Cuts at: 3006 3714 3809 3006 Size: 708 95 5681 Fragments arranged by size: 5681 708 95 MfeI C'AATT_G Cuts at: 4083 4083 Size: 6484 MluI A'CGCG_T Cuts at: 6360 6360 Size: 6484 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5598 5782 1305 Size: 4293 184 2007 Fragments arranged by size: 4293 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2289 10 Size: 54 1425 343 457 4205 Fragments arranged by size: 4205 1425 457 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2175 2319 2864 3546 4442 4801 4960 Size: 648 1009 144 545 682 896 359 159 Cuts at: 4960 518 Size: 2042 Fragments arranged by size: 2042 1009 896 682 648 545 359 159 144 MspA1I CmG'CkG Cuts at: 1606 2124 2535 2975 3117 3351 3696 3778 3900 Size: 518 411 440 142 234 345 82 122 Cuts at: 3900 4577 5725 5970 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 518 440 411 345 245 234 142 122 82 NaeI GCC'GGC Cuts at: 2212 2212 Size: 6484 NarI GG'CG_CC Cuts at: 3670 3670 Size: 6484 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5932 NdeI CA'TA_TG Cuts at: 387 387 Size: 6484 NgoMIV G'CCGG_C Cuts at: 2210 2210 Size: 6484 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6484 NotI GC'GGCC_GC Cuts at: 3931 3931 Size: 6484 NspI r_CATG'y Cuts at: 1132 1229 2280 3842 5387 1132 Size: 97 1051 1562 1545 2229 Fragments arranged by size: 2229 1562 1545 1051 97 PasI CC'CwG_GG Cuts at: 1348 3220 1348 Size: 1872 4612 PciI A'CATG_T Cuts at: 5383 5383 Size: 6484 PflMI CCAn_nnn'nTGG Cuts at: 1108 2563 1108 Size: 1455 5029 PmeI GTTT'AAAC Cuts at: 3788 3788 Size: 6484 PmlI CAC'GTG Cuts at: 2418 3231 3544 2418 Size: 813 313 5358 Fragments arranged by size: 5358 813 313 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4553 4585 6066 6098 4553 Size: 32 1481 32 4939 Fragments arranged by size: 4939 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3760 3760 Size: 6484 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6484 PsiI TTA'TAA Cuts at: 4054 4054 Size: 6484 PspOMI G'GGCC_C Cuts at: 3017 3017 Size: 6484 PstI C_TGCA'G Cuts at: 830 2321 3836 830 Size: 1491 1515 3478 Fragments arranged by size: 3478 1515 1491 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6484 PvuII CAG'CTG Cuts at: 2124 3117 3351 3778 2124 Size: 993 234 427 4830 Fragments arranged by size: 4830 993 427 234 SacII CC_GC'GG Cuts at: 2976 2976 Size: 6484 SalI G'TCGA_C Cuts at: 1936 3826 1936 Size: 1890 4594 SanDI GG'GwC_CC Cuts at: 3760 3760 Size: 6484 SbfI CC_TGCA'GG Cuts at: 3836 3836 Size: 6484 ScaI AGT'ACT Cuts at: 4732 6444 4732 Size: 1712 4772 SexAI A'CCwGG_T Cuts at: 2520 3024 2520 Size: 504 5980 SfcI C'TryA_G Cuts at: 826 1045 2119 2317 2900 3832 4967 5648 5839 Size: 219 1074 198 583 932 1135 681 191 Cuts at: 5839 826 Size: 1471 Fragments arranged by size: 1471 1135 1074 932 681 583 219 198 191 SfoI GGC'GCC Cuts at: 3671 3671 Size: 6484 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6484 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6484 SmaI CCC'GGG Cuts at: 1872 3811 1872 Size: 1939 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3769 4588 5489 5751 820 Size: 197 926 1826 819 901 262 1553 Fragments arranged by size: 1826 1553 926 901 819 262 197 SnaBI TAC'GTA Cuts at: 493 4275 493 Size: 3782 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6484 SphI G_CATG'C Cuts at: 1132 2280 3842 1132 Size: 1148 1562 3774 Fragments arranged by size: 3774 1562 1148 SspI AAT'ATT Cuts at: 5 52 4408 5 Size: 47 4356 2081 Fragments arranged by size: 4356 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6484 StyI C'CwwG_G Cuts at: 513 1065 2790 3923 513 Size: 552 1725 1133 3074 Fragments arranged by size: 3074 1725 1133 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2014 2165 2357 2560 2570 2608 3374 Size: 1255 434 151 192 203 10 38 766 Cuts at: 3374 3536 4511 4528 4681 4866 5311 325 Size: 162 975 17 153 185 445 1498 Fragments arranged by size: 1498 1255 975 766 445 434 203 192 185 162 153 151 38 17 10 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4730 6442 96 Size: 274 80 33 51 1443 2753 1712 138 Fragments arranged by size: 2753 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2295 2327 3548 3580 6444 6476 2295 Size: 32 1221 32 2864 32 2303 Fragments arranged by size: 2864 2303 1221 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6484 XbaI T'CTAG_A Cuts at: 3820 3820 Size: 6484 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 3149 3355 2011 Size: 1138 206 5140 Fragments arranged by size: 5140 1138 206 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6484 XmaI C'CCGG_G Cuts at: 1870 3809 1870 Size: 1939 4545 XmnI GAAnn'nnTTC Cuts at: 4613 4613 Size: 6484 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4292 276 Size: 53 83 186 936 2758 2468 Fragments arranged by size: 2758 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PspXI PsrI RsrII SacI SapI SfiI SrfI SwaI